sailfish: sailfish.xml annotate

annotate sailfish.xml @ 2:20eab032389f draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit b9ccc98180d52233931768511b909ccd6142f158

author	bgruening
date	Mon, 21 Dec 2015 14:00:53 -0500
parents	06646e81c543
children	03c74355227f

rev	line source
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	1 <tool id="sailfish" name="Sailfish" version="0.7.6.0">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	2 <description>transcript quantification from RNA-seq data</description>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	3 <requirements>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	4 <requirement type="package" version="0.7.6">sailfish</requirement>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	5 </requirements>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	6 <macros>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	7 <xml name="strandedness">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	8 <param name="strandedness" type="select" label="Specify the strandedness of the reads">
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	9 <option value="U" selected="True">Not stranded (U)</option>
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	10 <option value="SF">read 1 (or single-end read) comes from the forward strand (SF)</option>
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	11 <option value="SR">read 1 (or single-end read) comes from the reverse strand (SR)</option>
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	12 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	13 </xml>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	14 </macros>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	15 <stdio>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	16 <exit_code range="1:" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	17 <exit_code range=":-1" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	18 <regex match="Error:" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	19 <regex match="Exception:" />
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	20 <regex match="Exception :" />
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	21 </stdio>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	22 <version_command>sailfish -version</version_command>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	23 <command>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	24 <![CDATA[
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	25
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	26 #if $refTranscriptSource.TranscriptSource == "history":
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	27 sailfish index
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	28 --transcripts $refTranscriptSource.ownFile
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	29 --kmerSize $refTranscriptSource.kmerSize
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	30 --out ./index_dir
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	31 --threads "\${GALAXY_SLOTS:-4}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	32 #set $index_path = './index_dir'
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	33 #else:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	34 #set $index_path = $refTranscriptSource.index.fields.path
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	35 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	36
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	37 &&
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	38
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	39 #if $single_or_paired.single_or_paired_opts == 'single':
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	40
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	41 #if $single_or_paired.input_singles.ext == 'fasta':
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	42 #set $ext = 'fasta'
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	43 #else:
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	44 #set $ext = 'fastq'
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	45 #end if
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	46
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	47 ln -s $single_or_paired.input_singles ./single.$ext &&
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	48 #else:
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	49
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	50 #if $single_or_paired.input_mate1.ext == 'fasta':
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	51 #set $ext = 'fasta'
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	52 #else:
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	53 #set $ext = 'fastq'
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	54 #end if
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	55
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	56 ln -s $single_or_paired.input_mate1 ./mate1.$ext &&
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	57 ln -s $single_or_paired.input_mate2 ./mate2.$ext &&
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	58 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	59
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	60
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	61 #if $geneMap:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	62 ln -s "$geneMap" ./geneMap.$geneMap.ext &&
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	63 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	64
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	65 sailfish quant
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	66 --index $index_path
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	67 #if $single_or_paired.single_or_paired_opts == 'single':
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	68 --libType ${single_or_paired.strandedness}
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	69 --unmatedReads ./single.$ext
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	70 #else:
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	71 --mates1 ./mate1.$ext
06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	72 --mates2 ./mate2.$ext
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	73 --libType "${single_or_paired.orientation}${single_or_paired.strandedness}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	74 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	75 --output ./
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	76 $biasCorrect
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	77 --threads "\${GALAXY_SLOTS:-4}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	78
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	79 #if $fldMean:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	80 --fldMean $fldMean
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	81 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	82
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	83 #if $fldSD:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	84 --fldSD $fldSD
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	85 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	86
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	87 #if $maxReadOcc:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	88 --maxReadOcc $maxReadOcc
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	89 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	90
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	91 #if $geneMap:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	92 --geneMap ./geneMap.${geneMap.ext}
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	93 #end if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	94
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	95 $noEffectiveLengthCorrection
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	96 $useVBOpt
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	97 $allowOrphans
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	98
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	99 $unsmoothedFLD
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	100 --maxFragLen ${maxFragLen}
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	101 --txpAggregationKey "${txpAggregationKey}"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	102
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	103 ]]>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	104 </command>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	105 <inputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	106 <conditional name="refTranscriptSource">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	107 <param name="TranscriptSource" type="select" label="Select a reference transcriptome from your history or use a built-in index?" help="Built-ins were indexed using default options">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	108 <option value="indexed">Use a built-in index</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	109 <option value="history" selected="True">Use one from the history</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	110 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	111 <when value="indexed">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	112 <param name="index" type="select" label="Select a reference transcriptome" help="If your transcriptome of interest is not listed, contact your Galaxy admin">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	113 <options from_data_table="sailfish_indexes">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	114 <filter type="sort_by" column="2"/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	115 <validator type="no_options" message="No indexes are available for the selected input dataset"/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	116 </options>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	117 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	118 </when> <!-- build-in -->
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	119 <when value="history">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	120 <param name="ownFile" type="data" format="fasta" metadata_name="dbkey" label="Select the reference transcriptome" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	121 <param argument="kmerSize" type="integer" value="21" max="32" label="The size of the k-mer on which the index is built"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	122 help="There is a tradeoff here between the distinctiveness of the k-mers and their robustness to errors.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	123 The shorter the k-mers, the more robust they will be to errors in the reads, but the longer the k-mers,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	124 the more distinct they will be. We generally recommend using a k-mer size of at least 20."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	125 </when> <!-- history -->
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	126 </conditional> <!-- refTranscriptSource -->
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	127
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	128 <conditional name="single_or_paired">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	129 <param name="single_or_paired_opts" type="select" label="Is this library mate-paired?">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	130 <option value="single">Single-end</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	131 <option value="paired">Paired-end</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	132 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	133 <when value="single">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	134 <param name="input_singles" type="data" format="fastq,fasta" label="FASTQ/FASTA file" help="FASTQ file." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	135 <expand macro="strandedness" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	136 </when>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	137 <when value="paired">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	138 <param name="input_mate1" type="data" format="fastq,fasta" label="Mate pair 1" help="FASTQ file." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	139 <param name="input_mate2" type="data" format="fastq,fasta" label="Mate pair 2" help="FASTQ file." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	140 <param name="orientation" type="select" label="Relative orientation of reads within a pair">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	141 <option value="M">Mates are oriented in the same direction (M = matching)</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	142 <option value="O">Mates are oriented away from each other (O = outward)</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	143 <option value="I" selected="True">Mates are oriented toward each other (I = inward)</option>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	144 </param>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	145 <expand macro="strandedness" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	146 </when>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	147 </conditional>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	148
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	149 <param argument="--geneMap" type="data" format="tabular,gff,gtf" optional="True" label="File containing a mapping of transcripts to genes"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	150 help="Calculates the aggregated gene-level abundance estimations. This file should be eiher a GTF file or tab-delimited format
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	151 where each line contains the name of a transcript and the gene to which it belongs separated by a tab." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	152
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	153 <param argument="--biasCorrect" type="boolean" truevalue="--biasCorrect" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	154 label="Perform bias correction" help=""/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	155
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	156 <param argument="--fldMean" type="integer" value="200" optional="True" label="Calculate effective lengths"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	157 help="If single end reads are being used for quantification, or there are an insufficient number of uniquely mapping reads when performing paired-end quantification
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	158 to estimate the empirical fragment length distribution, then use this value to calculate effective lengths."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	159
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	160 <param argument="--fldSD" type="integer" value="80" optional="True" label="Standard deviation"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	161 help="The standard deviation used in the fragment length distribution for single-end quantification or when an empirical distribution cannot be learned."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	162
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	163 <param argument="--maxReadOcc" type="integer" value="200" optional="True" label="Maximal read mapping occurence"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	164 help="Reads mapping to more than this many places won't be considered."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	165
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	166 <param argument="--noEffectiveLengthCorrection" type="boolean" truevalue="--noEffectiveLengthCorrection" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	167 label="Disable effective length correction" help="Disables effective length correction when computing the probability that a fragment was generated from a transcript.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	168 If this flag is passed in, the fragment length distribution is not taken into account when computing this probability."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	169
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	170 <param argument="--useVBOpt" type="boolean" truevalue="--useVBOpt" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	171 label="Use Variational Bayesian EM algorithm for optimization" help=""/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	172
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	173 <param argument="--allowOrphans" type="boolean" truevalue="--allowOrphans" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	174 label="Consider orphaned reads as valid hits when performing lightweight-alignment"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	175 help="This option will increase sensitivity (allow more reads to map and more transcripts to be detected), but may decrease specificity as orphaned alignments are more likely to be spurious."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	176
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	177 <param argument="--unsmoothedFLD" type="boolean" truevalue="--unsmoothedFLD" falsevalue="" checked="False"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	178 label="Use the un-smoothed approach to effective length correction" help="This traditional approach works by convolving the FLD with the characteristic function over each transcript."/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	179
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	180 <param argument="--maxFragLen" type="integer" value="1000" optional="True"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	181 label="The maximum length of a fragment to consider when building the empirical fragment length distribution"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	182 help=""/>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	183
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	184 <param argument="--txpAggregationKey" value="gene_id" type="text" label="The key for aggregating transcripts during gene-level estimates"
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	185 help="The default is the gene_id field, but other fields (e.g. gene_name) might be useful depending on the specifics of the annotation being used." />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	186
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	187 </inputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	188 <outputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	189 <data name="output_quant" format="tabular" from_work_dir="quant.sf" label="${tool.name} on ${on_string} (Quantification)" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	190 <data name="output_bias_corrected_quant" format="tabular" from_work_dir="quant_bias_corrected.sf" label="${tool.name} on ${on_string} (Bias corrected Quantification)">
1 06646e81c543 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit 326fbf6e8070a792e3bda3256e5465c2d5b1eb71 bgruening parents: 0 diff changeset	191 <filter>biasCorrect is True</filter>
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	192 </data>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	193 <data name="output_gene_quant" format="tabular" from_work_dir="quant.genes.sf" label="${tool.name} on ${on_string} (Gene Quantification)">
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	194 <filter>geneMap is True</filter>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	195 </data>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	196 </outputs>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	197 <tests>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	198 <test>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	199 <param name="single_or_paired_opts" value="paired" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	200 <param name="input_mate1" value="reads_1.fastq" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	201 <param name="input_mate2" value="reads_2.fastq" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	202 <param name="biasCorrect" value="True" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	203 <param name="TranscriptSource" value="history" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	204 <param name="ownFile" value="transcripts.fasta" ftype="fasta" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	205 <output file="sailfish_quant_result1.tab" ftype="tabular" name="output_quant" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	206 <output file="sailfish_bias_result1.tab" ftype="tabular" name="output_bias_corrected_quant" />
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	207 </test>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	208 </tests>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	209 <help>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	210 <![CDATA[
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	211 What it does
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	212
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	213 Sailfish is a tool for transcript quantification from RNA-seq data. It
2 20eab032389f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit b9ccc98180d52233931768511b909ccd6142f158 bgruening parents: 1 diff changeset	214 requires a set of target transcripts (either from a reference or de-novo
0 3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	215 assembly) to quantify. All you need to run Sailfish is a fasta file containing
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	216 your reference transcripts and a (set of) fasta/fastq file(s) containing your
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	217 reads. Sailfish runs in two phases; indexing and quantification. The indexing
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	218 step is independent of the reads, and only need to be run one for a particular
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	219 set of reference transcripts and choice of k (the k-mer size). The
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	220 quantification step, obviously, is specific to the set of RNA-seq reads and is
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	221 thus run more frequently.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	222
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	223 When the quantification output contains a number of columns:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	224 (1) Transcript ID,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	225 (2) Transcript Length,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	226 (3) Transcripts per Million (TPM) and
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	227 (4) Estimated number of reads (an estimate of the number of reads drawn from this transcript given the transcript’s relative abundance and length).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	228
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	229 The first two columns are self-explanatory, the next four are measures of transcript abundance and the final is a commonly used input for differential expression tools.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	230 The Transcripts per Million quantification number is computed as described in [1], and is meant as an estimate of the number of transcripts, per million observed transcripts,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	231 originating from each isoform. Its benefit over the F/RPKM measure is that it is independent of the mean expressed transcript length
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	232 (i.e. if the mean expressed transcript length varies between samples, for example, this alone can affect differential analysis based on the K/RPKM.).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	233
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	234
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	235
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	236 Fragment Library Types
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	237 ======================
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	238
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	239 There are numerous library preparation protocols for RNA-seq that result in
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	240 sequencing reads with different characteristics. For example, reads can be
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	241 single end (only one side of a fragment is recorded as a read) or paired-end
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	242 (reads are generated from both ends of a fragment). Further, the sequencing
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	243 reads themselves may be unstraned or strand-specific. Finally, paired-end
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	244 protocols will have a specified relative orientation. To characterize the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	245 various different typs of sequencing libraries, we've created a miniature
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	246 "language" that allows for the succinct description of the many different types
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	247 of possible fragment libraries. For paired-end reads, the possible
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	248 orientations, along with a graphical description of what they mean, are
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	249 illustrated below:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	250
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	251 .. image:: ReadLibraryIllustration.png
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	252
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	253 The library type string consists of three parts: the relative orientation of
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	254 the reads, the strandedness of the library, and the directionality of the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	255 reads.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	256
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	257 The first part of the library string (relative orientation) is only provided if
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	258 the library is paired-end. The possible options are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	259
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	260 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	261
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	262 I = inward
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	263 O = outward
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	264 M = matching
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	265
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	266 The second part of the read library string specifies whether the protocol is
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	267 stranded or unstranded; the options are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	268
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	269 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	270
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	271 S = stranded
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	272 U = unstranded
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	273
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	274 If the protocol is unstranded, then we're done. The final part of the library
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	275 string specifies the strand from which the read originates in a strand-specific
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	276 protocol — it is only provided if the library is stranded (i.e. if the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	277 library format string is of the form S). The possible values are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	278
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	279 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	280
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	281 F = read 1 (or single-end read) comes from the forward strand
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	282 R = read 1 (or single-end read) comes from the reverse strand
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	283
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	284 So, for example, if you wanted to specify a fragment library of strand-specific
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	285 paired-end reads, oriented toward each other, where read 1 comes from the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	286 forward strand and read 2 comes from the reverse strand, you would specify ``-l
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	287 ISF`` on the command line. This designates that the library being processed has
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	288 the type "ISF" meaning, I\ nward (the relative orientation), S\ tranted
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	289 (the protocol is strand-specific), F\ orward (read 1 comes from the forward
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	290 strand).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	291
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	292 The single end library strings are a bit simpler than their pair-end counter
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	293 parts, since there is no relative orientation of which to speak. Thus, the
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	294 only possible library format types for single-end reads are ``U`` (for
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	295 unstranded), ``SF`` (for strand-specific reads coming from the forward strand)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	296 and ``SR`` (for strand-specific reads coming from the reverse strand).
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	297
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	298 A few more examples of some library format strings and their interpretations are:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	299
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	300 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	301
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	302 IU (an unstranded paired-end library where the reads face each other)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	303
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	304 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	305
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	306 SF (a stranded single-end protocol where the reads come from the forward strand)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	307
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	308 ::
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	309
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	310 OSR (a stranded paired-end protocol where the reads face away from each other,
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	311 read1 comes from reverse strand and read2 comes from the forward strand)
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	312
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	313 .. note:: Correspondence to TopHat library types
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	314
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	315 The popular `TopHat <http://ccb.jhu.edu/software/tophat/index.shtml>`_ RNA-seq
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	316 read aligner has a different convention for specifying the format of the library.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	317 Below is a table that provides the corresponding sailfish/salmon library format
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	318 string for each of the potential TopHat library types:
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	319
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	320
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	321 +---------------------+-------------------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	322 \| TopHat \| Salmon (and Sailfish) \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	323 +=====================+============+============+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	324 \| \| Paired-end \| Single-end \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	325 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	326 \|``-fr-unstranded`` \|``-l IU`` \|``-l U`` \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	327 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	328 \|``-fr-firststrand`` \|``-l ISR`` \|``-l SR`` \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	329 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	330 \|``-fr-secondstrand`` \|``-l ISF`` \|``-l SF`` \|
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	331 +---------------------+------------+------------+
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	332
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	333 The remaining salmon library format strings are not directly expressible in terms
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	334 of the TopHat library types, and so there is no direct mapping for them.
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	335
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	336
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	337 ]]>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	338 </help>
3b4ed0e473dc planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/sailfish commit bd2dd2419ea52f30cd7de2f7109a12b49b5d0dba-dirty bgruening parents: diff changeset	339 </tool>

Mercurial > repos > bgruening > sailfish

annotate sailfish.xml @ 2:20eab032389f draft