Mercurial > repos > bgruening > trim_galore
changeset 16:cd7e644cae1d draft default tip
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/trim_galore commit 276a0ec327f5369c16563696047f0d31577c353f"
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Fri Oct 08 09:57:52 2021 +0000 @@ -0,0 +1,66 @@ +<macros> + <token name="@TOOL_VERSION@">0.6.7</token> + <token name="@VERSION_SUFFIX@">0</token> + <xml name="requirements"> + <requirements> + <requirement type="package" version="@TOOL_VERSION@">trim-galore</requirement> + </requirements> + </xml> + <xml name="xrefs"> + <xrefs> + <xref type="bio.tools">trim_galore</xref> + </xrefs> + </xml> + <xml name="adapter_trimming"> + <conditional name="trimming"> + <param name="trimming_select" type="select" label="Adapter sequence to be trimmed"> + <option value="">Automatic detection</option> + <option value="--illumina">Illumina universal</option> + <option value="--nextera">Nextera transposase</option> + <option value="--small_rna">Illumina small RNA adapters</option> + <option value="user">User defined adapter sequence</option> + </param> + <when value=""/> + <when value="--illumina"/> + <when value="--nextera"/> + <when value="--small_rna"/> + <when value="user"> + <param name="adapter" type="text" value="AGATCGGAAGAGC" label="Adapter sequence to be trimmed off"> + <validator type="regex" message="Adapter sequence must contain DNA characters only (A,C,T,G or N)">^[ACTGNactgn]*$</validator> + </param> + <yield/> + </when> + </conditional> + </xml> + <xml name="paired_adapter_trimming"> + <expand macro="adapter_trimming"> + <param name="adapter2" type="text" optional="True" value="" label="Adapter sequence to be trimmed off read 2"> + <validator type="regex" message="Adapter sequence must contain DNA characters only (A,C,T,G or N)">^[ACTGNactgn]*$</validator> + </param> + </expand> + <param name="trim1" type="boolean" truevalue="--trim1" falsevalue="" checked="False" label="Trims 1 bp off every read from its 3' end." help="" /> + <param name="three_prime_clip_R1" type="integer" value="" optional="True" label="Remove N bp from the 3' end of read 1"> + <help>Instructs Trim Galore! to remove N bp from the 3' end of read 1 after adapter/quality trimming has been performed. + This may remove some unwanted bias from the 3' end that is not directly related to adapter sequence or basecall quality. + (--three_prime_clip_R1)</help> + </param> + <param name="three_prime_clip_R2" type="integer" value="" optional="True" label="Remove N bp from the 3' end of read 2"> + <help>Instructs Trim Galore! to remove N bp from the 3' end of read 2 after + adapter/quality trimming has been performed. This may remove some unwanted bias from + the 3' end that is not directly related to adapter sequence or basecall quality.</help> + </param> + </xml> + <xml name="citations"> + <citations> + <citation type="bibtex"> + @misc{githubTrim_Galore, + author = {Krueger, Felix}, + year = {2021}, + title = {Trim Galore}, + publisher = {GitHub}, + journal = {GitHub repository}, + url = {https://https://github.com/FelixKrueger/TrimGalore.com/fenderglass/Flye}} + </citation> + </citations> + </xml> +</macros>
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/mec_reads_pair1.fastqsanger Fri Oct 08 09:57:52 2021 +0000 @@ -0,0 +1,396 @@ +@M01368:8:000000000-A3GHV:1:1101:6911:8255/1:R1:ATCTGGTT:R2:TCGCCTTA:F1:CCTA:F2:CCGC +TTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT ++ +GGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB +@M01368:8:000000000-A3GHV:1:1101:14518:9998/1:R1:GTTATTAT:R2:CATCACGA:F1:TATG:F2:TGGA +CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT ++ +GGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A// +@M01368:8:000000000-A3GHV:1:1101:18422:19051/1:R1:GTATCCGA:R2:CTACAAGC:F1:CATC:F2:ATTA +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF. +@M01368:8:000000000-A3GHV:1:1101:25545:21098/1:R1:ATTAATTA:R2:GCCCATAA:F1:ACAC:F2:CACT +CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG ++ +GGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/A<C@EGFDCGGGHFHHHEGFGHBFHG0:CEHFCHGGED.;0CEF.F99B0CFFEEFGGG0FBFBBF0F/FFBDE?/9//9B.FFBFFFFFFBF..A..;@B-- +@M01368:8:000000000-A3GHV:1:1101:5446:12248/1:R1:AATTAACA:R2:ATCCGACA:F1:CACT:F2:TCTG +TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTAT ++ +GGGGGGGFGHHHHHGGGGHHHHHHHHHHHHHHHHGBGHGGHGGHHHHHHHHHHGHGHGGGGGHHHHHHHHGHHHHHHHHHGGGGGHHHHFFGHHHGGGGGGHHHGFGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGGHHHHHHHHHHHHHFHGGGHHHHGGGGGG:FE;EGEGGGGG/;?FGGGGGGGFFFFGGFFFFFFFFFBFFFFFFFFFFBFFFFFFEFFFFFEFFF +@M01368:8:000000000-A3GHV:1:1101:5861:6452/1:R1:ATTATGTC:R2:TTATCCGA:F1:CTAC:F2:CATC +AGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTT ++ +GGGGGGGGGHHHHHHHHHHHGHHHHGHHHHHHHHHHHGGGGHHHHHHHHFHHHHHHGGHGHGGHGGHHHHHHHGGHFHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHHEGGHHGGGGGGHHHGGGGHGGGGGHHHHHHGGGDCGHHHHGGGGGGGHEFGGGGHGHHHGHGGGFGGGGGGGEGGGGGGG?E0CEFGGGGGFEE9EEFFFFFBFFFFFFFBFFBD.AFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1102:10403:6021/1:R1:CGCTCCGG:R2:GGATGAGG:F1:GCCC:F2:CAGG +TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTG ++ +FGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE<CG1<GF0/DD000=<DHGBDFDCECE/:AC?-;-;9B/ABBB-AD9BFB99AB?BDFBAD-.9..@;=;;..9..9/9;BEF;A:9/BFF +@M01368:8:000000000-A3GHV:1:1102:10677:23253/1:R1:CCTTAAAT:R2:AATTAACA:F1:AAGA:F2:CACT +ATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCTGTCTCTTATACACA ++ +GGGGGGCGGGHHHHHGHHHHHHHHHGHHHHHHHHHHHGHHHHHGGGGGHGHHHHHHHHHHHHHBGFHHHHGHGHGGHGGGCGGGHHHGGGGGGGHHHGHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFDFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFEDBDFFFFFFFEFFFE0F0FBFFFF0FFFFFFFFFFFFFFFFF: +@M01368:8:000000000-A3GHV:1:1102:13809:1733/1:R1:ATATTGAA:R2:NATAAAAC:F1:CGTA:F2:CTAA +GTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGAGGGCTATTTAGGTTTTATGCTGTCTCTTATACACATCTCCG ++ +GGGGGGGGGHHHHHHHHHHHGHHGGGGGH3EHHHGHGHHHHHHHHHGGCEGGGHHHGGGFHGGGGGGHHGEAEFHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGCFFGHHFGHHHGHHHHHHCHGFHHHHGGHGGG00EHGHHHGFHHGEHGHFFFHHGGFHHHGGGGGGGGGB;;CFFGGFGFFBFFDFED;E.:A?DFFFFFFFF:FFFFF0BFFBBBFFFFFBFF0BFBF9F. +@M01368:8:000000000-A3GHV:1:1102:17584:10050/1:R1:ATCAAAGA:R2:GTCATAAA:F1:CAGA:F2:ACCT +ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT ++ +GGGGGGGGGGHHHHHGGHHHGGGGGGHHHGFGDHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGEGGHHHHHHHGHGHHGHHHHGHHHHGGGGFDGHFHGEHHHHHHGFFHHHHHHHHHHHHHHHHHGGHHHHHHHHHHFHHHHHHGGGGGHHHHGGGHHGHHHGGGGGGGGGGGFGFFGGGGGGGCFGGGFGGGGGGGFFFFFFFFFFFFFFFFFFF;.BF9ADF/9;A;DFFF0 +@M01368:8:000000000-A3GHV:1:1102:18842:24844/1:R1:CACTTGGG:R2:CTACAAGC:F1:GGTA:F2:ATTA +CTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCT ++ +GGGGGGGGGHHGHGGHHHGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGGHHHHHHHHHHHHHHHHHHHHGGGHGGHHHHHHGHBFGHHHHHHHHBGHHHHHHGHGGGHFGCGGFHHFHFFHHBHHHHFFHFHHHHGGDHGGBC?;@DFBFFFFFFFFFBFFFFFFFFFFFFFF.B?FB.@;;DFFFFFFFFFFFEE-A./BBBFBFBFFF//BFB/BFF/ +@M01368:8:000000000-A3GHV:1:1102:21788:11027/1:R1:GCCCACAC:R2:AATTAACA:F1:GTTC:F2:CACT +CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCTG ++ +GGGGGGGGGGHGHHHHHHGGHGGHHHHHHHHGHHHHHHHHHHHHHHHHHHGHHHHHFGGGGHHHHHHHHHHHHHHHHHHHHHHGHGHGGFCGGCGGGHHHGGGGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFDFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEF9EFFFFFFFFFFFBFF0FFBBBBFBFBFFFF0 +@M01368:8:000000000-A3GHV:1:1102:5728:8777/1:R1:ATATTGAA:R2:CACTTGGG:F1:CGTA:F2:AGTA +GTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG ++ +G5GGGGGGGHHHGHHHHHHHHHHGGGGGHHHHHHGHHHHHHFGHHHGGGGGFHGHGGGHHHGGGCEGHHGG?F1BGGGGGHHHHHGGGGEGGHHHHGGGGGCGHGGGGCGHHHHHHHHHHHHHHHHGGHHHHGGCEEDFDEGGHHHHHHHCEHHFHHHHGHGFHHHFFGGGFFFGGFGBGFFFFFFFFGFDGGD9EFA.DAFA/;FEFF9FB?/BBF/FFFFBBFFFFF/FFEFFFBB +@M01368:8:000000000-A3GHV:1:1102:5983:21093/1:R1:GTATCCGA:R2:ATTGAACG:F1:CATC:F2:TAGG +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +GGGGGGGGGHHHHHHGFHHHHHHHHGGHHHGHGHHHGGGGFHHHHHHHHGHHHHHHHHHHHHHGGHGGHHHHHHFHGGHHHHFHGHHEHHHHHHHGHHHHHD@FGGHHHHHFFFHHGGHGH?DHHHHHGHGHEGG/@?ADGFGGFFFFAFFFFFFFFFFFFFFFFFFFFFFFAAFFFFFFDFFFFFFFF;D9BFFFFFFFFFFFFFFFFFEFFFBBFFF0BFFFBBFF0EFFFFFFFA +@M01368:8:000000000-A3GHV:1:1102:7765:18353/1:R1:CTCCGGGC:R2:ATTATGTC:F1:CCAT:F2:CTAC +ACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTC ++ +GGGGGGGGGGGGGGHHHHHHDHHHHHHHHHHHGGGGGHHGHGHHHGHHHHHHFHHHHGHHHHHHGHGHHHGHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHGFFHHHHHGHHHHHHHHHHHGHHHHHGCFGGHGHFHHGHGHGHHHHGGFHHHHGGGHGBFGCC=BFFFFFFFF-@DFFFFFFFFFFFFFFFFFFFFFFAFFAEF.::FBFBFFE-A9:BFFFF0 +@M01368:8:000000000-A3GHV:1:1103:10405:17879/1:R1:GTATCCGA:R2:CTACAAGC:F1:CATC:F2:ATTA +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +GGGGGGGGGHHHHHHHHHHHHGHHHGHHHHHHHHHHHGGGGGGHHHHHHHHHHHHHHHHHHHHGGHGGHHHGHHGHHGHHHHHHHHHCGGFHHHHGHHHHHGCFGGHHGHHHHHHGFHHHGHHHHHHHGHGHGFF/CC?ADAFGFFFFFFFFFFFFFFDFFFFFFFFAFFFFFFFFFFFEDFFFFFFFFFFAFFFFFFFFFFFFFFFFFFFFFFBBFBFBFFFFFFFFFFFFFFFFF? +@M01368:8:000000000-A3GHV:1:1103:13493:8205/1:R1:GTAGCTAA:R2:AACGTAGG:F1:AGTG:F2:TGCG +ACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT ++ +GGGGGGGGGGGGGGHFHHHHHHHHHHHHHHHHGHHHHHHHHGHHHIHHHHHHHGGGGFHHHHHHHHHHHHHHHHHHHGHGGHGGHHHHHHHHHGHHHHHHHHHHHHHHHHHGHHHH/FGGGGHHHHHHHGGEHHHHHGHHHHHHGHGHEFEFGGFFFFFFFFFFFFFFFFFFFFFAFDFFFFFAFFFF;;@FFEFFFFFFFFFFE=?AFFFFFFFFFFFFFFFFFFBFBBBFFFFEFF +@M01368:8:000000000-A3GHV:1:1103:14060:21273/1:R1:GTATCCGA:R2:TAATAGGA:F1:CATC:F2:TGAG +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTACTGTCTCTTATACAC ++ +GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHGHHHHGGGGGFHHHHHHHHHHHHHHHGHHHHGGHGGHHHHHHHHHHHHHHHHHH?GGHHHHHHGHHHHHFEGGGHHHHHHHHHHHHHHHGFHHHFHGHGHGFGCGG@@BCFGFFFFFFF?FFFFFFFFAFFFAD?@FFEEFFFFFFFFFFFFFFFFFFD9BFF/B/FFF/:/9FB9/BFA//;BFB:FFFBB/BFFFFFBFFFFB/ +@M01368:8:000000000-A3GHV:1:1103:14528:12993/1:R1:GCCCATAA:R2:CTTTAGTA:F1:CACT:F2:AGTA +GGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT ++ +FBCG2EGFFGHHCF5AFHHHFHFHHHGG?CEHBHHDGFHHGHHGHBGHABFFBGHGDGGGFHGHHGGHHHGGG@E1GEHHHGHHFHH3FEDHHHHGFGHGFHHGHFBGA?FGHHHHHGHHFFGHFDFGGHHH2??<ECFHHGHHHHHHHHHGHH<1DFGHGC<.FCB/<=@DCFFFFFEDA=-AAB9B/9B9B-@?.-;A-AFF.=-=BFEFEDEFFFBFEA-A.B//9F9F//;BBB +@M01368:8:000000000-A3GHV:1:1103:17657:20856/1:R1:AAAGCGAA:R2:CTTTAGTA:F1:CTGT:F2:AGTA +TCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTAT ++ +GGGGGGGGGHHHHHHHHGHHHHHHHHHHHHHHHHHGHHHHHHGHHHHGGGGGHHHHHHHGHHFHHHHHHHHHHGGGGGHHHHHHHH3BGGHHHHHHHEHHHHHHHGHHHHGEEGGGHHHHHHHHGHHFHGHGHGHGHHCHGGECCDFGB@AFFFFFFFFFFFFFFFFFDAFFFFFFFFFFFFFCAF;AFFFFEFFBBEFFF9FBBFFFFFFFFFFEFFFFFFFFFFFFF/FFFFBFF9 +@M01368:8:000000000-A3GHV:1:1103:23099:3794/1:R1:AAGACAGA:R2:CTTCAGGG:F1:TACT:F2:TCAT +CGACATAGGGTGTTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCTCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGTCT ++ +GEEGGFGGGGCFGEHCGGGGGGFHFFEDEHFGGDEHHHHHHGGFECGHHHHGFEFGCGHGGFGGGHBGFH3/BF3GGGHHEEEHHG22???BCFFFFFFHHFHFF@FGHH11GHHHHFGFHHHHGGHHHHEFFGFGHFG1DGFGGGGEDCCCAEGGFFHHFHHHHHHCFBBFGBFFF/0;B0B00;B0BBFGBB9BFGEFGGGFFDEEAB;BDDBFBFFFFF@;.9BF..;-./-A=F +@M01368:8:000000000-A3GHV:1:1103:25465:20010/1:R1:GACATAGG:R2:AACCTAAA:F1:GTGC:F2:TAGC +CCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATCGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAACAGAC ++ +GGFEFGGGGHHFEFFHFGGGGGHHHGHFGGGGGHHHGGGGGGGGHGEEEGGHHHGFHEHFHDGHGHEHGF?FGG?GGGGHHGHF4F?2?DDCDHGHHHHHFG2@FGHHHGH?CFGGHFHFHHHHGDDGGHGGGGGHGFH.<AFAGHHHFHGHHFCGFHHHHHGHHHHBGHHGGDGAGF.CEGCFDGGGGGEFFF=B.FFFEFFFFFFADF?/9//BFF.B/;:>---;--..;.../9 +@M01368:8:000000000-A3GHV:1:1103:25519:6301/1:R1:AAATAAGA:R2:CTGTAATA:F1:CATC:F2:TTGA +CGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT ++ +GG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/<A@DFBBEGC--:;GHHHGBECG@:9-9.-9C/ABB?B@?.EDAFFFFBAFBFBFBFFFFF//;BBFFFF/;/9BBF/BBFBFFFFFFFFFEFF??BF/.AF./;B/9FFFFFFBFBFFFBFFFBFFFFF/BF/A---9.9A-B?DFFF/B/B?---.//;. +@M01368:8:000000000-A3GHV:1:1103:6074:6549/1:R1:TAACACTT:R2:TTAGTAAG:F1:GGGG:F2:TATG +TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG ++ +GGGGGGGGG5GFHHHHHGHHHGGGGGHHHGHHHHHHHHHHHHGHHHHHHHHHGGHHHHGHHHHHHGGGGHHHHHHHHHHHHHHGHHHHHHHGGHGGHHHHHHHFGHHGHGHHHHHHHHHHHHHGHHHHHGGGGCHHHHEHGHHHEGHHHFGGHHHHGHGHGHF@CCBDFAFFFFFF-CDFFFFFFFFFFFFFAFFFFFEFF;BFF?FEDFFFFFFFF-A./FFFFFFFFFFFF/BFF/ +@M01368:8:000000000-A3GHV:1:1104:11572:8264/1:R1:GTATCCGA:R2:CATTCAAT:F1:CATC:F2:TGTT +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +GGGGGGGGGGHHGHHGHHHHGFGCHGHHGFFHEHHHHGGGGFGHHHHHHHHHHHHGHHHHHHHGGGGGHHHFFHHGHHHHHHHHHHHEHHHHHHHGHHGHGGEGEGHHHFHHHHGHHHHHHGGHHHHHGHGEGEHCGG-BGDFGFFFFAFFDFFFFEFFCFFDDFFFFFFFEC>;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9 +@M01368:8:000000000-A3GHV:1:1104:11928:24197/1:R1:ACGTAGGT:R2:CCTCGCTC:F1:GCGA:F2:CGGG +AAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATA ++ +GGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D9 +@M01368:8:000000000-A3GHV:1:1104:12288:20635/1:R1:CACTTGGG:R2:CCTACAAG:F1:GGTA:F2:CATT +CTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT ++ +GGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9 +@M01368:8:000000000-A3GHV:1:1104:13832:4398/1:R1:CACGATGG:R2:GACAGATA:F1:ATCA:F2:CTGC +AGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC ++ +GGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.- +@M01368:8:000000000-A3GHV:1:1104:23670:4161/1:R1:ATTCAATT:R2:CGACATCT:F1:GTTA:F2:GGTT +TATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC ++ +GGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB +@M01368:8:000000000-A3GHV:1:1104:24927:22253/1:R1:AGCTAAAG:R2:GTAATATT:F1:TGAA:F2:GAAC +TGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT ++ +GGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB +@M01368:8:000000000-A3GHV:1:1104:4705:7727/1:R1:CTACAAGC:R2:TCCTACTT:F1:ATTA:F2:CAGG +TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG ++ +GGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF +@M01368:8:000000000-A3GHV:1:1104:7756:4776/1:R1:GGCCCATA:R2:CTACAAGC:F1:ACAC:F2:ATTA +TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGA ++ +GGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF/ +@M01368:8:000000000-A3GHV:1:1105:11310:21761/1:R1:ATAAATAA:R2:ACTTGGGG:F1:TAGG:F2:GTAG +TGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT ++ +GGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0 +@M01368:8:000000000-A3GHV:1:1105:11578:14676/1:R1:ATTGAACG:R2:GCTCCGGG:F1:TAGG:F2:CCCA +GCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC ++ +GGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1105:13575:19255/1:R1:TTAGTAAG:R2:GGTTCCTA:F1:TATG:F2:CTTC +TCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +G3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11</?//<@?/F<<<A-<FBGH0.00=G0<<C//./0;;GC....<C.=.00;;BB./:.900////.C000;0090;..//000;C/;;/B/9BA9A9-AEEFF?B---;B99//9/;A//9 +@M01368:8:000000000-A3GHV:1:1105:15502:11453/1:R1:GTCCTACA:R2:GATCACAG:F1:AGCA:F2:GTCT +TAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCTGTCTCTTATACACATCTC ++ +GGGGGGGGGHHGHHHGHHHHHHHHHFGHHHHHGGGGHGHHHHHGFHHHHGHGGGHHGGHGGHHHHHHHHHHHHHHGGGGGFHGHHHGHHHHHGHHHHGGGGGGHGEGFECHEHGGGGGGHHHGADFHHGGGGHHHHH<ECGGGGHHHHGGGGGGGHGGGGGGHHHGHGHHGEHBFFFGFGEGGGGGEDEGF0C;;BFFFGGGBEFFE/FFFFFFFFFFFFFFEFFFFFFFFBFFFBFF +@M01368:8:000000000-A3GHV:1:1105:16787:9771/1:R1:GTTATTAT:R2:TCCTACTT:F1:TATG:F2:CAGG +CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT ++ +GGGGGGGGGHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIHHHHHDHHHHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGGHHHHHGGGGGGHHGHGGGGGGGEEGEGFGHHHGGGGFFGGGGGGGGGGGGGGGEG?G;:EFFFFFFFFF:AEFFFFFFFFBFFFFFFFFFEFF/ +@M01368:8:000000000-A3GHV:1:1105:17564:15723/1:R1:TATGCTCG:R2:ATCCGACA:F1:CCTG:F2:TCTG +AATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAA ++ +GGGGGGGGGHHHHHGFGHGGHGGHHHHHHHHGHHEHHGGGGGHHHHHHHHHGHHHGHHHHGGGGGHHHHFFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHFEHHHHHHHHHHGHHGHGGHGGGCGGGGHHH1FGGGFGGFGGGGGGGGGGEFGGGGGGGGGGGGGGGGGFFFFFFFFFFAEFFFFFFFFFFBBFBBFFFFFE1:BFFB0 +@M01368:8:000000000-A3GHV:1:1105:18108:12091/1:R1:TAATAGGA:R2:GTATCCGA:F1:TGAG:F2:CATC +CAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACAC ++ +GGGGGGGGGHHHHHHHHHHHHHGGGGGGFHHHBFGHHHGGGGGGHHGGGGGHHGFGGHHHHHHGGGGGGHGHFGGGGGGDGEGEFGCHHHHHHHHHHGHHHHGEDHHHGGGCDGGGGFHFHGHHHGHHGHFGHHHFHHGFHHHFHHGGHFHFHHGB0CGHGFHCBGGGGEDEE9ADFEFFFGFBFGFFGGFFFFFGGFGFFFFFFFF/A?FEFFFFFEF-A.B//;BFFEF//BB/// +@M01368:8:000000000-A3GHV:1:1105:18331:16683/1:R1:TCTGGTTC:R2:TTATTATG:F1:CTAC:F2:TCCT +TCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTA ++ +GGGGGGGGGHHHHHHGHGHHGGGHHGHGGGGFFHHGHHHGHHHHHHH3FGHHGGGHGGHHHHFHHHFHGHHHGHHHHHHHHHHHHGHHHHGGCGGGHHGHHHHHHHHHHHHHFHHGHHGHGHEFGGGGABFFFGBEGGAFFFFFFFFFFFFFDDFF?DFFFFFFFFAEFFFFFFFFFFFFB;9FFFFFEFFFFFFFFFFFF?FFFFFFF/BFFFFFFFB.DABFFFFFFB.//:FB// +@M01368:8:000000000-A3GHV:1:1105:20577:18421/1:R1:GGGGGTAG:R2:ATGTCCTA:F1:CTAA:F2:CAAG +GTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCC ++ +EAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/: +@M01368:8:000000000-A3GHV:1:1105:21099:2895/1:R1:TAGCTAAA:R2:TTATTATT:F1:GTGA:F2:ATGT +CTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTT ++ +GGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF0 +@M01368:8:000000000-A3GHV:1:1105:26469:19245/1:R1:TTATGATT:R2:GGGGTAGC:F1:ATGT:F2:TAAA +CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG ++ +GGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD<EHHGGGGGHHGHH?EDCCDHHHHDGFFGGGHGGFCGGHHHHFG.CFGGGGGGGGGGGBFGDEG.AFFG;AFFFFEB?/A9BBBFFFFFFF/9FFFFBDEFFFF +@M01368:8:000000000-A3GHV:1:1105:7169:17145/1:R1:AACCTAAA:R2:CATTCAAT:F1:TAGC:F2:TGTT +CACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGT ++ +GGGGGGGGGHHHHHHHHHHHBHHHHHHHGGHGGHHHHHHFHHHHHHHHHHHHHHHHHHHHGHHHHHGGGGCHHHHHHHHGHHHHHHHFHHHHHGHGHGHHGGGGGFGGHHGGGGGGGGGGGGEGGFGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFDFFFFFFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFF-9@DDBFFFFFFFFBBFFF0 +@M01368:8:000000000-A3GHV:1:1106:15481:16176/1:R1:CTTTAGTA:R2:AAAGTGAA:F1:AGTA:F2:CTGT +GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTT ++ +GGGGGGGGGHHGHGHHHGHHHFGHGFGHFEHGGHGGHHHGHHHHFGHGGFGFFHHFHHHHEGHFHHHHHFGGGGGHGGGHGFHGHGGGGGGHHHGGGGHHGGGGHHHGGHGGGGGGHHHHGGGGGCFHGGGCDGHGHHHFHEHHHHHHHHHEEFHHGG:C:?0<EFGBFGGGGGFE.BFGEFFFGFFGGGGGGFDEF/FFGFFFFFFF;BBFA;.:EFFE:AAFEF.BFFFFF;9FF. +@M01368:8:000000000-A3GHV:1:1106:17389:18671/1:R1:CCATAACA:R2:GTATGTTC:F1:CTTG:F2:GCCT +GGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGGCTTTGATTC ++ +GGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE</ECDFHHBF1DGBFBFGFH<1GHGDGGFDFE0/<--;-9./BBED;BFBD/BF9BFFFDBFDADD;FFF?->;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/ +@M01368:8:000000000-A3GHV:1:1106:22927:3863/1:R1:GTATCCGA:R2:AAGACAGA:F1:CATC:F2:TACT +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA ++ +GGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9/// +@M01368:8:000000000-A3GHV:1:1106:23625:4236/1:R1:TAGCTAAA:R2:CACTTTAG:F1:GTGA:F2:TAAG +CTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTA ++ +GGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHG<C???B=@ABBFEF@=-AFFFFFFBFAFFDFFFFA?AFFCFFABEFFFFFFFFF?FFFDFFBFF;FFEFFEFFFFFFFAF///9FF/BB/ +@M01368:8:000000000-A3GHV:1:1106:26031:14826/1:R1:GATCACAG:R2:CTGTAATA:F1:GTCT:F2:TTGA +TCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGTCTTCTGCTTGA ++ +GGGGGGGGGHHHHHHGHHHHHGGGGGHHHHHHHHGHGHHHHHHHHHHHGHGHFHFGE?CGDGGHHGGGGGGGHHHHGHGGGGGGFGGGHGHGGGGGGGGGGGGGGGGGGDGAFFGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFB/9FFBFFFFFFFFFFFFFD@D=BEFFF;FDDB////B=CF-.;BAFFFFFF;;BFE/BFFFFB: +@M01368:8:000000000-A3GHV:1:1106:27607:15990/1:R1:GACAGATA:R2:CCCCTTAA:F1:CTGC:F2:ATAA +ACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGACTTCTGCTTGAAAAAAAAAAACTACAACCCACGGACGCA ++ +GGGGGGGGGGGHHHGGGGGGHHHGGGGGFCEGGHHHGHHGGGEGGHHHAFGGGGGGAFG//EGHHHHCGEHHHHEEHHFHGHFHHGGEF/FGGC?FDFH2DHHFFGGFGHHHHGGHHFGHHGHGHHGFHDHBGBBGHHFHHGGCCDC<=D<DGHHC;CGFCCFBECDG?FGGA-;EB9FBGFFBBF>DFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9--- +@M01368:8:000000000-A3GHV:1:1106:6455:17124/1:R1:GTATCCGA:R2:GCATTAAT:F1:CATC:F2:TAAT +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +GGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9 +@M01368:8:000000000-A3GHV:1:1107:14451:26748/1:R1:GTATGTTC:R2:AGCTAAAG:F1:GCCT:F2:TGAA +TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGA ++ +GGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9. +@M01368:8:000000000-A3GHV:1:1107:18714:24788/1:R1:GTATGTTC:R2:GGCCCATA:F1:GCCT:F2:ACAC +TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGA ++ +GGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF/ +@M01368:8:000000000-A3GHV:1:1107:21355:8333/1:R1:ATATTGAA:R2:GTATCCGA:F1:CGTA:F2:CATC +GTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG ++ +GGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9 +@M01368:8:000000000-A3GHV:1:1107:22864:15712/1:R1:AATAAGAC:R2:GTAATATT:F1:ATCA:F2:GAAC +GATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG ++ +GGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A// +@M01368:8:000000000-A3GHV:1:1107:24817:14233/1:R1:CTTCAGGG:R2:CTTTAGTA:F1:TCAT:F2:AGTA +AAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT ++ +CFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/ +@M01368:8:000000000-A3GHV:1:1107:24830:14440/1:R1:AACTGTAT:R2:CTTTAGTA:F1:CCGA:F2:AGTA +ATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC ++ +GGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC- +@M01368:8:000000000-A3GHV:1:1107:26478:23602/1:R1:GCTAAAGT:R2:ATCAAAGA:F1:GAAC:F2:CAGA +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC ++ +EGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//</ECCC>1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9 +@M01368:8:000000000-A3GHV:1:1108:11059:23181/1:R1:GTTATTAT:R2:AACTGTAT:F1:TATG:F2:CCGA +CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT ++ +GGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/ +@M01368:8:000000000-A3GHV:1:1108:12233:13498/1:R1:TAGGTGCG:R2:GGTTCCTA:F1:ATAA:F2:CTTC +TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC ++ +GGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/<GHHFGBGFHFBGHHHGHHGEFHFHFGBF0BFGGGFGGGGGEFFF;A?EEG?CFFFFF0:BEFFFFFBFFFFFF0FFEFF?EFFFF0BFFFFBBB00 +@M01368:8:000000000-A3GHV:1:1108:14328:14751/1:R1:ATAATAGG:R2:GTATCCGA:F1:ATGA:F2:CATC +GCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACA ++ +GGGGGGGGGHHHHHHHGHGHHHHHGGGGGFHHHFFDGHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHGHGGGGGGGGGGGCFGHHHHHHHHHHHHHHHHHGHHHHGGGDGGFGGFGGHHHHHH>FHHHFHGHGHHGHHHGHHBA<CGFHHHHHHHHHHGFHHGDDEGCEGGGGEEFBFGGGGFGGGB0CFF:FFFFFBBFFFFBFEFA.BFFFEFFFBF/BF/FFEFFBFFF/ +@M01368:8:000000000-A3GHV:1:1108:16350:22621/1:R1:TTAGTAAG:R2:CATCTGGT:F1:TATG:F2:TCCT +TCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +GGFGGGGGGHHHHHHGBGGGFGGGHFGGGGGHHHHHGGHHHHFHGGGGGGHHGFHHHHHGEFHHFFHHGFCEFHHHGFF?GHHEE@ECFHHEGGGGBFFEGGGHFDHHGFG/BFEHHFCDGGGCDEGGGCGFFFHHHFEGHFHHHHGHHDGHHHGGHDED0CE;CFFFFBFF/;CBFFFGG0/FFF0B0:BB?.;BFF0FGFBFFFFFEFE;ADED.;BE.99FAFFFFFFF/;EFF/ +@M01368:8:000000000-A3GHV:1:1108:21791:23272/1:R1:CTTTAGTA:R2:CATAACAC:F1:AGTA:F2:TTGG +GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTA ++ +GGGGGGCGGGHHHHHHHHGHHACGDAGFEEHGGHHGHHHHHHHHHHHGGGGGGHHHHGFHFGHGFDDGFFFGGFGHHFF?FEGHHGGGGGGHHHGFFDHHFGG?HEFGHHGGGDDCGFFC?ECCFGDEFGGGGGGFHHHHHHHEFHFCGHHGEGGGGD.:.A;CECAFFFFBFF/;EFFBFFBFB;;BFFFFBBAA./BFFEBB//BFFFFFFFDFFFEEFEFFFBFBFBBFFFFF./ +@M01368:8:000000000-A3GHV:1:1108:23314:26089/1:R1:ATTGAACG:R2:CATAACAC:F1:TAGG:F2:TTGG +GCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTG ++ +FBFGGEGGGGHGHHFHHHGHHGFGGFHHHHHHHHFHHFHHGHHHGGGGGHHHHFFEHHHGGGGGGHFHGEEGF3FEFGHHHHHFFDEEEEHHHGEEGGGGGHFGGDADGFFFFFHHFGHHHFGBHGHHGBCEHDEDDGADCFFHHHGG/:GCCCGHFGHFFGG0CGFGF.FB/9;BFBB0FFBFG0A-..;C:E/.A9.FFFFFFFF9BFFFFFFBFBBB./:BFFF/F;AA?F//// +@M01368:8:000000000-A3GHV:1:1108:25084:18127/1:R1:TTATTATG:R2:CCCTTAAA:F1:TCCT:F2:TAAG +CAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGAGATAGACCTGTGATCCATCGTGATGTCT ++ +GGGGGGGGGHHHHHGHHHHGFGGFHHHHGHHGGFHEHHHCGGGHBHHHHHHFHHGHHHHGGHHGGHGGGHHHEFHHHHEHGHGGGCFHFHHHHHHHHHHHGHEFGDEGGGHFHHCGGHHGGGGGFGHHGGEGDFHEGG/?GFHGHDGGGGGHHHHADFGGGGGEGCGGGEFGHHFEBFFGGG?GGGGEGGFEFAA?.0:A0;BFFFBFF/;9BFFFFEFF9FBFFB/F/.;FFFF/BB +@M01368:8:000000000-A3GHV:1:1108:7626:10048/1:R1:ATCAAAGA:R2:AACCTAAA:F1:CAGA:F2:TAGC +ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAA ++ +GGGGGGGGGGHHHHEFEFHHGGGGGGHHHGEF?GGGGGGHHHHHHGGGEGEHHHHGGGGGGGGGGGGGGHHHHHHHHHHHHFFCGHGHHHHGG/BFGDGG2FFHFHGHH0FHFGFHHHHFFHHHHHHHGG<FHHHFGHHGGGGFHHHGGGGGGHGGHGGGHHHHHHFGGGGGGGFFFGGFGGBFGGGGGGGGGGGFGFFFCFFDFFFFFFFFF;./;AAAFFFFFFAFABBFFFB/;; +@M01368:8:000000000-A3GHV:1:1109:11270:12255/1:R1:AATCAAAG:R2:CTACCTCA:F1:ACAG:F2:GGGT +TACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAGGTAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTT ++ +GGGGGGGGGGGHHHHFFFHHHGGGFGGFFHGEGCHHGGGGHHHHHHGGGGGGHHGHGGGGGGGGGCEGFCHHHHHGFHFHHHHGFHHGGFHGGGFD/EFGEFFDGFHHGEFCEHFFGHHHGHHHHHHHHHGCGHHHGGFGHHHHGHEHHCFFADGHHHD?CG.CGHBCBFBFFGGFFFGFFFFF.CFBFBCFFFFGFFFFFGGGFFFFFDFAFFFFFF-@DBFFFFBFD---.;BAA. +@M01368:8:000000000-A3GHV:1:1109:19224:11655/1:R1:AAATAGCC:R2:CCTGTAAT:F1:CACA:F2:ATTG +GTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGCATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCTGTCTCTTATACACATCTCCGAGCC ++ +GGGGGGGGGHHHHHHHHFHHHHGFGHGGGFFAGFHHHFHHHGHHHHHHHHHHHFHGGHGHHGCFFGHHHGHHHGHHFHGGH3BFFGHHGHGEFFH/EG@-CFGGCC@-;BD?FGGGGGG??FGDAGDFFFFEFF@BAAFEFFBFFFFF?FD9BBFFFF/FFFFBFFFFFFF?FFBFFFF/FBBFFBFFFE?F;@B//AFFFFFFFFF//;BFFEF.BFFFB/;F/99/FBFFBDCF;B +@M01368:8:000000000-A3GHV:1:1109:21978:9490/1:R1:GGGTCATA:R2:GAATCAAA:F1:AAAC:F2:GACA +TAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCAGTT ++ +GGGGGGFFGHFGCEGGFHHHHHHHHHGHCBGFEGGHHDEGAFGHGHFGDGBCGHHHHHFHHGHHHHHHHGHHHH1E?EGGGHHHHFGFHFHHHHHEDHHGHH@GGHGHHCGG?CFGFGGGGGFGB.BFGGFFGGFFFBDDBFFFFFF9-;DEFFDFBFFBB.EFF;BFBFFFFFFFFFFFFFFFFFFFFFFFFF/B9/FFFFFFFAFFFF--99AFFFFFFFA;;;FF..9//;/;;/ +@M01368:8:000000000-A3GHV:1:1109:22910:4079/1:R1:TAATAGGA:R2:CTCCGGGC:F1:TGAG:F2:CCAT +CAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTCCACTTTAGCTA ++ +GGGGGGGGGHHHHHHHHHHHHHHGGGGGHHHHGGEHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGFHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGGHHHGGHGGGHHGHGHHHHHHHGFHHHHHHHHHHHHHHHHHHGGHHHHHHHHHHGHHHHHHGGGGGGGGGGGGGGGGGGGGFFGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFCCDFFFFFFF0BFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1109:24217:19077/1:R1:AAAGTGAA:R2:TAATAGGA:F1:CTGT:F2:TGAG +TCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGCCTTTGATTCCTGCCTCATCCTATTACTGTC ++ +BFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9////99/ +@M01368:8:000000000-A3GHV:1:1109:25237:11722/1:R1:CCTGTAAT:R2:CATCACGA:F1:ATTG:F2:TGGA +ACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT ++ +5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGEC<CDFHHHHHE.FD11FFAGBGD.DGHEA<.<<0<EHAHCFHHF./;CC0GFH0::0CFGG0FBFD;9/90BFF0CBBFGB09;B:0;CFF0.-;@AFEEF--;AFFBF/99D-;.//.../9;B-. +@M01368:8:000000000-A3GHV:1:1109:27103:7153/1:R1:GTAATATT:R2:NTACTTCA:F1:GAAC:F2:GGGT +TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGCTGTCTC ++ +CG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.<GGEHGF0CHHHF/CAGC000CCGFHFGFGFFG?:CB990;F09;FB0BBGGGGGG?;A9EBDEFA///;FF/:F?BFBBFFBFFFF///;/9B;/// +@M01368:8:000000000-A3GHV:1:1109:8763:7859/1:R1:TAGTAAGT:R2:GGTTCCTA:F1:ATGT:F2:CTTC +CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGCTTTATGC ++ +GGGGGGGFBGFGHHFBHHHHGGHHGGEHGDHHHFGHEHHHHGHHGGGGAGHHFBHHGHFHHHHHHHHGGGCEFGFFFFEHHH?FFG1FFHHFEGGGHGFEEHHHHHGGGFGGGHHHHGGGGG/C/FG-<A<GFHHFFCGHHFHGE0<<CGHHHGCHG.CCCCCE00BBFGFFG.BB0FFBFBCF0;CBFFFEEGBBBFFBF/FFF9BF/9BAFFD9AFEDAA.A/BFFFFFFFFFEB/ +@M01368:8:000000000-A3GHV:1:1110:12808:18367/1:R1:AATTAACA:R2:GCCCACAC:F1:CACT:F2:GTTC +TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCCTG ++ +GGGGGGGFGFGHHGGGGGHHHHHHHHGFHHGEGGGHHGFGHEEGGHHHHGHHGFFHHGGGGGHHHHGHGHGHHHFGGFHHGGGGGHHFFFGGHHHE?@EGGHFHEGGGHHGGGGHGEFHHGD?/>CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9 +@M01368:8:000000000-A3GHV:1:1110:14751:26540/1:R1:TAACACTT:R2:ATGTTCGC:F1:GGGG:F2:CTGT +TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG ++ +EEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;/// +@M01368:8:000000000-A3GHV:1:1110:15519:10008/1:R1:CTGTAATA:R2:GGTTCCTA:F1:TTGA:F2:CTTC +CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC ++ +GGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA; +@M01368:8:000000000-A3GHV:1:1110:16559:7507/1:R1:CATTCAAT:R2:GCCCACAC:F1:TGTT:F2:GTTC +TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC ++ +GGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9 +@M01368:8:000000000-A3GHV:1:1110:16700:6843/1:R1:AAATAATA:R2:GTTCCTAC:F1:GGAT:F2:TTCA +AGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC ++ +GGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD +@M01368:8:000000000-A3GHV:1:1110:18014:7856/1:R1:ATAAATAA:R2:CATCACGA:F1:TAGG:F2:TGGA +TGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG ++ +GGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A<GFHFHHHGHBBGFEGGGGHFFFEF/EEGGHGGFEHFHHBA.<ADFFHFHB/CCGHFEFHHGHGEFFGGGGGFFFGGFGFFBFFGGBFGG??DGG?@9-ADFFFFB/@BD;./BFE?BFE/>99FE//BBFFF.9/FF?DA=@9./9 +@M01368:8:000000000-A3GHV:1:1110:23303:11846/1:R1:ACTTGGGG:R2:GTCCTACA:F1:GTAG:F2:AGCA +TAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC ++ +GGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B; +@M01368:8:000000000-A3GHV:1:1111:13195:14562/1:R1:ATCAAAGA:R2:GTATCCGA:F1:CAGA:F2:CATC +ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGA ++ +GGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BFA +@M01368:8:000000000-A3GHV:1:1111:14287:20817/1:R1:GCCAACAC:R2:ATTCAATT:F1:GTTC:F2:GTTA +CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT ++ +GFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9 +@M01368:8:000000000-A3GHV:1:1111:23624:14516/1:R1:GTCCTACA:R2:CGACATCT:F1:AGCA:F2:GGTT +TAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG ++ +GGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/ +@M01368:8:000000000-A3GHV:1:1111:7437:24508/1:R1:ACACACTT:R2:ACATCACG:F1:TAGT:F2:ATGG +AGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG ++ +GGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>- +@M01368:8:000000000-A3GHV:1:1111:8665:25542/1:R1:TCCTACTT:R2:CTTTAGTA:F1:CAGG:F2:AGTA +TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG ++ +GGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A- +@M01368:8:000000000-A3GHV:1:1111:9655:13916/1:R1:GGCCCATA:R2:TTAGTAAG:F1:ACAC:F2:TATG +TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGA ++ +GFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG<GDHBFDGFFCGH//B//?0GFFGFGGHHHFHFB.=1FDFB?FGH.C/.-:::CH0;CAB@-.;0CGFFFGG--9.A@GB>--99@=---AAABA-BF/B?A@F-9BBFF//BB/9/ +@M01368:8:000000000-A3GHV:1:1112:10272:7828/1:R1:GTATCCGA:R2:GTGCTCCG:F1:CATC:F2:GCTC +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT ++ +GGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A. +@M01368:8:000000000-A3GHV:1:1112:9832:16531/1:R1:CTGTAATA:R2:GTATCCGA:F1:TTGA:F2:CATC +CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC ++ +4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//.. +@M01368:8:000000000-A3GHV:1:1112:9832:6701/1:R1:CGCCTGTA:R2:GCTAAAGT:F1:ATAT:F2:GAAC +GAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGA ++ +GGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C<EGCECEFCFFHBGHE:GHHGFF0FFFB0CFGGGGAGFFF0;;FFGGGFFGFFCDD.DEFBEDD?9FFFFFFFFFFFFFFF/B./FB///;BF/ +@M01368:8:000000000-A3GHV:1:1113:13532:8618/1:R1:GGAATCAA:R2:GCCCATAA:F1:AGAC:F2:CACT +GATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGCTACAGTTCACTTTAGCTACCTCCAAGTGTTATG ++ +GGGGFBFGGGGGGH5GHFEDHHGGGGCGFBGH?AEEHFFEEGHGHHHHGGG?0EHGHHGGGGGC?GEE/EE?GHHHGBHFHHHHHHGHHGHHHGGGCG/A@<FGGHHHHHHHFF/GFDGHHHHHHGFFFGHGGHABGHFHHHHHFHEGDGHGDDEEHHBEDDEGFGHGBFFBFFEGGGEFGFGFB000;F0F;FGGEGGEFFF;-.9//B///B9FFFFF/BFFB/:.BB//;FBFFF +@M01368:8:000000000-A3GHV:1:1113:21679:18011/1:R1:GATCACAG:R2:TTAGTAAG:F1:GTCT:F2:TATG +TCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGAGGGTGTGCACGCGATAGCATTGCGAGACGCTTGAGCCGGAGCACCCTATGTCGCAGTATATGTCTTTGATTCCTGCCTCTTCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACTGTCTCTTATACACATATCCACGCCCACGAGACTAAGGCGAAGTTCATT ++ +FFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0<FFDGGCC?FF221<BDD11@<11@DDCGEF1<111F1<F1FBGHGHCF-CHHFA./</0CGHF<0CC/;C-:-;;09;FFBFBBFFBC0FFFGGFC0009C00090/-:--9--;-;AFFE;/99-9--/;/// +@M01368:8:000000000-A3GHV:1:1113:25528:14016/1:R1:CCTACAAG:R2:GTATCCGA:F1:CATT:F2:CATC +ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGG ++ +GGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF +@M01368:8:000000000-A3GHV:1:1113:5741:16959/1:R1:TAGTAAGT:R2:GTATCCGA:F1:ATGT:F2:CATC +CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGA ++ +GGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0<C;CGGGGEFF/.;0;FFFBF/0;0CFGFFB..9B/;0CBFFBBFFFFBAC?DED9;B9AD;.FFFB/B/;FBA/B// +@M01368:8:000000000-A3GHV:1:1114:10130:11959/1:R1:CGTTCCCC:R2:ATCAGAGA:F1:TTAA:F2:CAGA +TAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTCTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAACAAACACACATCCAGA ++ +FFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9;BF9FF9FFFFFFFFFFFFFF?BB?;9EE;-;DDB9//;B-B@;ABFFEFFFF/-.9A.;//9/BF./B/..9.9:...////// +@M01368:8:000000000-A3GHV:1:1114:14540:5315/1:R1:CTTTAGTA:R2:CACTTGGG:F1:AGTA:F2:GGTA +GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTT ++ +GGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE. +@M01368:8:000000000-A3GHV:1:1114:15066:16302/1:R1:TAAATAAG:R2:TTATTATT:F1:ACAT:F2:ATGT +ACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATTGCTTGTAGGACA ++ +GGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<<AHHHGGGGGGGFHH0GHFCCCADGGG?.9/A-???DGGFFF.9F9/EE-;;BBBFFBFFFFFFFFFEFFFFBFFBBFFFFF/BFFBFFFFF-DBFFF;/BFF//BB//9/BEA---9:BFFFFFF/F/.;.:@9.BBFF/;BFF/;/////9/////. +@M01368:8:000000000-A3GHV:1:1114:16639:15258/1:R1:CCTAAATA:R2:TTATTATT:F1:GCCC:F2:ATGT +CACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGCGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTCCAATATTACAGGCGAACATACTTACTAAATTGTGT ++ +GCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/; +@M01368:8:000000000-A3GHV:1:1114:2404:13066/1:R1:TCCTACAA:R2:ATGGATCA:F1:GCAT:F2:CAGG +AATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC ++ +GGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/mec_reads_pair2.fastqsanger Fri Oct 08 09:57:52 2021 +0000 @@ -0,0 +1,396 @@ +@M01368:8:000000000-A3GHV:1:1101:6911:8255/2:R1:ATCTGGTT:R2:TCGCCTTA:F1:CCTA:F2:CCGC +ACTCACCCACGGCGGCCATCAGCCGATACTAAGTTTGGGGTATGGTGGGGGGGATAATGAATTAGGTTGTGGGGGAGGGTTTGTGGTTGAGAGAAACACAAAAAACAATCTTATATATGGGTAGTCGTTTTGTATTGGTTTTTTGTTTTGTTTGTGTTTTGAGTGTCGGTTTAGTTCGGTGTACTAGGGGGGGTGGATGGGGTCGGCTGGTGAGGGGGTCTTAGTGTATTGAGTGTGG ++ +A111A100000000////011110//>>/12@1@22B/////1@>21/>>/-----9/;////9////--;-;-;-----;--------9/-/-///9-;-------9//////9/////-//-/9-;-;9--/////99-;--9-:-;----/---/-----////---9-/////--;A-//////---------9/-----;-----/-/-----;--;//////////9;///- +@M01368:8:000000000-A3GHV:1:1101:14518:9998/2:R1:GTTATTAT:R2:CATCACGA:F1:TATG:F2:TGGA +CACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACATAATAATAA ++ +GGGGGGGGGHHHHHHHHHHHHHHHHGFHHHHGGGGGHGFHHHHHHHHHHHHHHHHHHHGHGGEHGGGGCGGGHHGGCGGGGGHHGHHHGGGGGGGG.BFFFGAGADFGAFDGFGGCFFF;DDFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFF09FFFE00;BE@;DABBFFFFFBBFB00;F:9;FFBFFF9BFFFFFFFFFFFFF90/::BFFFBF0 +@M01368:8:000000000-A3GHV:1:1101:18422:19051/2:R1:GTATCCGA:R2:CTACAAGC:F1:CATC:F2:ATTA +TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG ++ +GGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHEHHHHHHHGGHHGGHGGHHHHHHHGHGGHHHGGGGGHGHHHHGGGHFHFHHHHHGGGGGHBFFCGDHHHGGGGGGHGGGGGGHHGCGGGFGHHBGGGGGFFFHEGGGGGCDCCE@EFGHHHHFHEGHGFFHHGB;ECBFGGGEFEFFGF0AFGFGFFG.;;DFFFFFFFFFF090BFFFE?FEFBBFBFFFB990BF +@M01368:8:000000000-A3GHV:1:1101:25545:21098/2:R1:ATTAATTA:R2:GCCCATAA:F1:ACAC:F2:CACT +GGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTTCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCAACCTATGTCGCAGTATCTGTCTTTGAT ++ +CGGCGGGCGHHHGHHHHCGHFGHFHGFGGCDGGBD5FGFFFHFHGGGHAEBFGHHHEHGGFHBGGFHHHGGGEEFHHHFHFFHDHGHGHHHHFFHHGGFAEGFFFFFBGHHHFFHHHHHHHFHFHHFHGFHGHGA/<@EFHEHH1GGHFFFHG1>=FGHFHGF.GE//-<BB?9.9BFFFB==AA/FFFFFA-@BA.;;D?F9FAB;---./99BFFFBBAE-.9B/BFB9F/9BFBB +@M01368:8:000000000-A3GHV:1:1101:5446:12248/2:R1:AATTAACA:R2:ATCCGACA:F1:CACT:F2:TCTG +TTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT ++ +GGGGGGGGGHHHHHGHHHHHHHHGHHHHHHGHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHGFGHHHHHHHHHHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGGGGGGFFFFFFFFFFFFFFAFFFFFFFFFFFFFBFC?.EAFFFFFFFFAFFFFFFFFFFFFFFFEFFFF0FFFFFFF0BFFFFFFFFF?.BDCFFEDA/ +@M01368:8:000000000-A3GHV:1:1101:5861:6452/2:R1:ATTATGTC:R2:TTATCCGA:F1:CTAC:F2:CATC +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHGGGGHHHHHHHHGHHHHHHHHHHHHHGGHGGHHHHHHHGHHHHHHHHHHHHHHHHHHHGGHHHHGGGGGGEHGHHHHHHHHHHHGHHHHHHGHGHGHGGGGCGGGGGFFFFFFFFFFFFFFFFFFFFFFFF?FFEAF:DAEF9DEFFFFFFF-A.BFFFFFEFFFFEFFFFFFFF0FFEB0FFFFBFFFFFFADEFCF0/. +@M01368:8:000000000-A3GHV:1:1102:10403:6021/2:R1:CGCTCCGG:R2:GGATGAGG:F1:GCCC:F2:CAGG +ATCAAAGACAGATACTGCGACATAGGGGGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGGGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGAGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACGCTGAAGTAGGAACCAGATGTCGGATACAGTTGACTTTAGGTAACCCA ++ +BGGGGFFCGGHHA5DFGAE?AAEDFHG2AEE1FBE00A1BGE0?E1FFGEEEGB4F43?EE/>///F??//@BCBAC<<BAGHB?11F/FGFGGBFBGBD.11><.<<<C0<A</<<=0GFHC@-.;FF09BFFGB00B0;FE.C/:CCF0;0C0;BBBFF@.9AF.:9EFF.;.BFF/FF/;/./BFB/..;;ABF/9FF//.;AAF9BBBA;-A.B/9///9/BF////;///:9. +@M01368:8:000000000-A3GHV:1:1102:10677:23253/2:R1:CCTTAAAT:R2:AATTAACA:F1:AAGA:F2:CACT +TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGCTGTCTCTTATACACA ++ +GGGGGGGGGHHHHHGGGGHHHHHHHHHHHHHHHHGGHHGGHGGHHHHHHGHGHGHHHGGGGGGGGHHHHHFHHHFHHHHGGGGGGHHGHHGGHHHGGGCGGHFFFGGGHHGGGFHHGHHHGGGCGGHFHHGGGGGGFHGC<@GGHGHHCHE00:CFHHHFGACGFFEG:D.E;FG;EFBBFGGGFEE0CBBBFFGBFFGFGFFFFFFFF0:B0:BFFFFFFF/0.;:0F0B:BFFF00 +@M01368:8:000000000-A3GHV:1:1102:13809:1733/2:R1:ATATTGAA:R2:NATAAAAC:F1:CGTA:F2:CTAA +TAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATCTGTCTCTTATACACATCTGAC ++ +GGFGGGGGGGGHHHHHHHHHHHHHHHHHHGHHHGGEGGFFHHHHHHGHGHGHHHHHEHFHGHHHHEFHHGHGGGGGHHHHGHHHHHHHHHGFGHHHHHGHGHGHHGGGCCGGGGFGGGGGGDGGGGFGDGGGFFFFFFEFFFFFFFBAF:9AF//;BEF-DD/FFFFBFFFFFFFFFEBFF?FB;/;//BFFEFFE/::..9;-:B9:FBFFFFFFBFB/9/FFF/B///99/:B/// +@M01368:8:000000000-A3GHV:1:1102:17584:10050/2:R1:ATCAAAGA:R2:GTCATAAA:F1:CAGA:F2:ACCT +AATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGATCTTGGGGGGTGG ++ +GGGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHFHGGHGGHHHHHHHHHHHHHHGHHHGGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHHGGHHHHHHGHGHGGHGGFGGGGGGGGGGFGGFGGGGGGGGGFFFFFFFFFFEFFCFAAFEFEFFFFFFFCFDFFFFFFEFFFFFFFFFFBFFFFFFF/:BFFFFFFFFFFFFFFFFAFC=@-.;A///B././//////.-9---- +@M01368:8:000000000-A3GHV:1:1102:18842:24844/2:R1:CACTTGGG:R2:CTACAAGC:F1:GGTA:F2:ATTA +TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG ++ +GGGGGGGGGHHHGHHHHHHHHHHHHHHHHGGGGHHHHHHHHFHHHHHGHGHHHGGHGGHHHHGHHHHHHGHHGGGGGHFHHHFGHHHHHHHHHHHGGGGGHHHGFECGGHGGGGGGHHHGGGGHHGGGGHHHHHEFDCADGHHHHGGGGFC?AE-@DDFHFFHHHCFHGGFFGFFFGGGEFEGFGED9C?CEGF90FEF/A./BBF9FFFFFFFFBFFF.9AF////;FFBFFB/:BF +@M01368:8:000000000-A3GHV:1:1102:21788:11027/2:R1:GCCCACAC:R2:AATTAACA:F1:GTTC:F2:CACT +TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCCTG ++ +GGGGGGGGGHHHHHGGGGHHHHHHHHGHHHHHHHGGHHGEHGGHHHHHHHHHHHHHHGGGGEHHHHHHHHHHFFFFFHHHGGGGGHFHGBFEHHHGGGGGGGHHGGGGHGGGGGHHFHHHGGG@CGHHHGGGGGGGGGGGEGDGFFDHHGEHHBCFHGBGFAGGHFGGCEEF0BE<?FFFGFGG<EFFGFFFFGGGGGF:0BFFB9FFFFFFFFFFFFF0B0ABADEF.A/..D.BA. +@M01368:8:000000000-A3GHV:1:1102:5728:8777/2:R1:ATATTGAA:R2:CACTTGGG:F1:CGTA:F2:AGTA +CTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCC ++ +GGGGGGDGGHHHHHFHHHGGGGGHHGFHFHHFHHHGGHHHHHHHHHHHHHGHHHHHHFGHHHGGGGHHHHHHHHHGHHHGHHHHFHHHGGHGGHFFHHHHGFHHHHHHHHHHFHFHHHFHFHHHHHGGGGGHGHHHHHGHHHHHHH/FHHHHHGHGGGHHGGFBFFFFFFFFFF;DDFFFFFFF;;BA.=DDBFFFFF;-DFF.EDFFFFFFEF-AFFFFFB;FBFFFBFFFFB9B.. +@M01368:8:000000000-A3GHV:1:1102:5983:21093/2:R1:GTATCCGA:R2:ATTGAACG:F1:CATC:F2:TAGG +GCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTG ++ +GGGGGGGGGHHHHHHGHHGHHGGGGGHHHHHHGHHHHHGGHHHHGGGGFHHHHGFFHGHGGGFEGGHFFFE?GFFGGGHGHFGHGGGEEFHHHHGGGGGCDGGBGGGGHHHGGHGHHHDGHHHHEFHHHHGF<A.ADGG0EHFFHHHHCEFHHEGHGHHGGHHHFGGGGEF//FFFFGGGFGGGGGGG.ACDE?B?DD9F.BBBBFFFFFEF0090:BF/;:0;0009:A?...0:;0 +@M01368:8:000000000-A3GHV:1:1102:7765:18353/2:R1:CTCCGGGC:R2:ATTATGTC:F1:CCAT:F2:CTAC +AGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAAAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTT ++ +BEGBGGGGGFHFHHHCFFFGFHHHHF5FEDABFAGGBEE22FFGGEEHF5EDCA11FE5FFE?FEG5EG555F@35B3FFA1FEF2@3GGD3?23?3@F4BF3E>///FDFACF?FHFGC//??1B0<<?/@GFGD/@1FG10<?ACC.<<G0DGGG--.<CCCCGC;CGCGFE9;0FBFA000.;0BF.9....0;909B00999A/9./;BBFB/.:B/////9/999//B//;/; +@M01368:8:000000000-A3GHV:1:1103:10405:17879/2:R1:GTATCCGA:R2:CTACAAGC:F1:CATC:F2:ATTA +TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG ++ +GGGGGGGGGHHFEHHHHHHHHHHHHGHHHGGGGHHHHHHHHEHHHHHFHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHFHHHFFGHHFGHHHGGGGFHHHHGGGHHGGGGGGGHGHGGGDHGGCGFHHGHHHGGGDGGHHHHGGGGGGGHGG-ADGGHHHBHHHFFFHHHGGGGEGBFGGAEEDBFF0C;CFFGFGFEEFFBBFFEFFFFFFFFFFDEFBFFFF///BFF/BF/A +@M01368:8:000000000-A3GHV:1:1103:13493:8205/2:R1:GTAGCTAA:R2:AACGTAGG:F1:AGTG:F2:TGCG +TAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGAT ++ +GGGGGGGGGHHHHHFHHGGGGGHHHHHHHHGHHHHHHHHHGGGGGHFHHGGGHHHGGGGGGHHHGGGDHHGGGGHHHHHHGGFEEEFFHGGGGGGCDHFGCCDGHGHHHHFFFEGHHHFCFEHGHHGG.C.EGGGFGFGEHGHH/:CGHHHGGGB::CGHHHHHGFGBBF0BFGFGGGGBFGGGBG.9.;BEG?AF.FFFFFFFFFFFFBBBFFFFF/BFFEFBBFFEFFFBBB;D9. +@M01368:8:000000000-A3GHV:1:1103:14060:21273/2:R1:GTATCCGA:R2:TAATAGGA:F1:CATC:F2:TGAG +CAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACAC ++ +GGGGGGGGGHHHHHHHHHHHHHHGGGGGHHHHEFGGHHGGGGGGGHGGGGDHHGGGGHHHHHHGGGEGFEHHHGGGGGGGAFGGGGBHHHHHHHHHGHHHHHHHGHEHHGGGGAFHFEGGHHFHHHF>FF<F1GGHHHHHHGHHGFFDEHHHHGF0GGHHHGFHGHFAGEGCEBEGF.FFFGGGGGFFGG0CBFBFGGGFFFFFBFFF.E.E?FFFFEFADAF/BFBFFFFFFFFF// +@M01368:8:000000000-A3GHV:1:1103:14528:12993/2:R1:GCCCATAA:R2:CTTTAGTA:F1:CACT:F2:AGTA +GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCCACGTGATGTCTTAGTTAAGGGGAACGGGTGGGCTCTTTAGGTTTTT ++ +GGGGGGGGGHHHGHHHFHGHHHHHGFHHGGHGGGHHHHHHHHHEEHGGGGGGHGHHHHGBGCHFHHHHHHGGGGGHGFHGFGHHHGGEGFGHHEGFFGHFEGG?HEBFHHF?FGGGEDG0CFGGG?E..<<-.CGGHHH0GHEGGDDC0/CA.0G/:;.;.;;;C09;BBFGBA.--./9;0C0/00;0;/./99.:9///:/9//;//;BF.D.AAD.;--@B9../;;:BF/;/.. +@M01368:8:000000000-A3GHV:1:1103:17657:20856/2:R1:AAAGCGAA:R2:CTTTAGTA:F1:CTGT:F2:AGTA +GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGAACTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTT ++ +GGGGGGGGGGHHHHGHFHHHHHHHGFGHGEHGGHHHHHHHHHEHFHHGGGGGHFHHHHFHHHHFFHFHHHGGGGDGHHGHFGHGHGGEEFGHHBFGGGHHGGGGHHHHHFFGGGGGHGHGDGCGGCGFF@DGGGHFHH0C.GHFHFHGHHFGH0CCAECDEG0CEGGGGFFFGG;C.0;;00:BF;FFBFFF09A9A:BFFFBFF0BF0:FFFAA;.AAEFFFFEF?FFFEFF9BBE. +@M01368:8:000000000-A3GHV:1:1103:23099:3794/2:R1:AAGACAGA:R2:CTTCAGGG:F1:TACT:F2:TCAT +AAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAACACCCTATGTCGCAGTATCTGTCTTCTGTCTCTTATACACATCTGACGCTGCCGACGACCGATCTAGTATAGATCTTGGGTGG ++ +GGGGGGGGGGGBFHHGG?EEHHGHHHHHHGFHFHHHHGHGHHGGHEEHFFFGHGHFGFHHHHHHHHHHHFHFFHGCGHGHGGG/EF3FGHHHHHHGHHHGFGHHHHHGHGFFEHCFGGGGGGGFFFG?FGGGGFFFGFA>>DFCFDFFFFFFFFF-BBAFFFFFFFFE@D9B;FBFFFFFFFFB9/9FFFEBF9/B/BB/BFAADDFFC-;;9-;@@9-.9/://///://///...- +@M01368:8:000000000-A3GHV:1:1103:25465:20010/2:R1:GACATAGG:R2:AACCTAAA:F1:GTGC:F2:TAGC +CACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGGTCTTGGTGGTCGTCCTCGTATTTGAACACATAATCGTG ++ +GGGGGGGGGHHHHHHGHHHGFHHHHHHHGGGGGHHHHHGHHHHHHHHEHHHHHGHGGHHGGGHHHHGGGGGHHHHHEHFGEHHHEE14BGHHHGHGHFCE/EFGGGGGGHGGGGGG?FFGGGG.ADGGGFFDA.FFEC--;9BBBD?FFFFFFFFBBFFFFFFFFFFFFFFFFFADFDFFC-BBB;BF-.;9:B9B9/9/;9;//;:.99/;-...:.../;//////.9/////.;. +@M01368:8:000000000-A3GHV:1:1103:25519:6301/2:R1:AAATAAGA:R2:CTGTAATA:F1:CATC:F2:TTGA +CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATGCTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGATGTCTTATTTCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGAT ++ +FFGGGCAFGCGHGGGHGGGHHFHHHFHHGGGGGFFFHHHBHHHHG5BG5GFEEEE1FGFFGF1EGHGGG/BFHEF@EA1BE1@EED3?4DHGGGGEGHFFFDDG?DCDEG@<<ACEHFHHGFDD1FGGGGFHGAGFHGDCAEFGDC0;BGFFBGA/.:GBC0;GBB9FGGGBFEG?.--AEBB00CBFFGBBF0000B09/B/9;/BF/BF.;A..AB;--9-;9-.9A/B/////// +@M01368:8:000000000-A3GHV:1:1103:6074:6549/2:R1:TAACACTT:R2:TTAGTAAG:F1:GGGG:F2:TATG +TCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +GGGGGGGGGHHHHHGFGHHHHHGDGHGGGGGHHGHHHGHHHHEHHF1EEFHHHHHHHGBGHFFFGFHHEFGC1EFGHFCEFGHDGC/EEGHFCEFEHFEGGEHHHHFFGDGCDGHHEHGD?C@<AC<CF?AF1GHHHHHHHGEHHF<CGFGHHHE?.CEGCCF0.CBBFGF?9:;0FFFE0;FBFBB009CFD?:/FGFBFBBFBF/B//FC.;.:9.;EFF...9FFFFB/:/A/// +@M01368:8:000000000-A3GHV:1:1104:11572:8264/2:R1:GTATCCGA:R2:CATTCAAT:F1:CATC:F2:TGTT +TTATCATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC ++ +GGGG5EGGGHHHHHHHHHHHHHHHHHHHHFFHHHFGHHHHHHFHHHGHHHHHDGGGHHHHHHHHHHGGHHHGGEGHGGHGGHHHGFHHHHHGHHHGGGFGHHHHHHGHHEHFHHHHHHGGFGEHGFHGCFHHGGGCFGEFHEGGGFHFGGGGHGHHHHGGGGFGHHGGGG@CGGG.C-C:9BFGGGGGGGGGGEFGFFB.ABFFGDGDF?/;AFFFBBFFFF/A./////;FB//;FB +@M01368:8:000000000-A3GHV:1:1104:11928:24197/2:R1:ACGTAGGT:R2:CCTCGCTC:F1:GCGA:F2:CGGG +CCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTAT ++ +GGGGGGGGGHHHHHGGGGGHHHHHHGHHHHBGHHHHHGGGGGHFGHGHHHHHHHHHHHHHHHHGHHHHGGHHHHHHGHHHHGGGGHHHHHHHHHFHHEHHHHHHHHHGGHGGHHHHHHHHFHHHHHHHHHHHHHHHHHHGHHHHHGGGGGHHHHHHHHGHHHHHGGHHHHCHGHGHGCCEGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFA:.AADEFFFBFEF;DAF: +@M01368:8:000000000-A3GHV:1:1104:12288:20635/2:R1:CACTTGGG:R2:CCTACAAG:F1:GGTA:F2:CATT +ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGG ++ +GGGGGGGGGHHHHGHHHHHHHHFHHHFGHF?FGGHGHHGHHHHFHHHHHFEGFHGGHDGHHHHFHHHGHFFHHGAEEGHCEHHHBGHHHBGHFGHHGGGGGFGHHFGCHHCEGE/FGHGHGGGDGGGGGDHFGHHH/E?@DDHBHH>?@DGCAGC-;@DGFFHGCHEEFGGFGFB/;..BBB.CCE?A0FE0EBFF0FEF.;=DFFB//BFBF//;/:9BA.9FB9//;9//;9F;FF +@M01368:8:000000000-A3GHV:1:1104:13832:4398/2:R1:CACGATGG:R2:GACAGATA:F1:ATCA:F2:CTGC +ACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGGGGGTGCCGGCTCATTAAAAAAAGAAGATGTCGAGATGTCAGTGCGTGAGTGGAAGTG ++ +GGGGGGGGGGGGHGGGGGGGHHHGFGGHHFGGGHGHHGHGFGECGHHHHGGGGGGGHEGFGGGHHGHFHHHHFGHFHHHHGHHHHFGGFGG3FFGGHHHHHHH2?FFGHFHHHHHHHHHBGHDGC0FGHHGHHGHHHHHHHHHHFGHHGGGGGHG-:@?FAGA:.:09CB0000;9B0.9-;---:./----.0;B00;:0;;.../90009..-..00000000-...90:.00/00 +@M01368:8:000000000-A3GHV:1:1104:23670:4161/2:R1:ATTCAATT:R2:CGACATCT:F1:GTTA:F2:GGTT +CTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAA ++ +GGGGGGGGGHHFHHHHHHGHGHHHHHGCHHHHGGGGHHHHHHHHHHHHHHHGHHGHGHGGHGGHGHGGHHGFGHHGHHHEHHGHHHHHGHEHEHHHGGFGGHHHFHHHGHHHHHHHGHHHHG4EGGGFCBBCA?DGGGGGGCCAFFFFFFFFFFFFFFFFFDFFF.9@--=AFFFF;FBFFBEFFF9FBFFB9/FFFFFFB/FFFFFFFBFFFF/BBFBB/FFFE?.;@FFB?FBB// +@M01368:8:000000000-A3GHV:1:1104:24927:22253/2:R1:AGCTAAAG:R2:GTAATATT:F1:TGAA:F2:GAAC +TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGGGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACGAG ++ +AFGG5GGGGHDGHHHHHHHHHHHHFHCFGC2F3BFGFGHBGHHFHEDHFGGGGGHHHG3F?GHHGGC/EFEHFGAEFFHGGAEHGGGG3EEGEEDHFHAAD@DGGGHG/<C/CHHHHHHEDHFHGHHG0>AC1>GED-<<-.;C0:0C:C;GH:::::0B90;FFGEGFBFF0C.;9F0;0090;900;00FA9D.A...-9=BBFFFE/FE9;9E//BBBB.BBB///B/9/B..;. +@M01368:8:000000000-A3GHV:1:1104:4705:7727/2:R1:CTACAAGC:R2:TCCTACTT:F1:ATTA:F2:CAGG +TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG ++ +GFGGFGGGGGHHHHFHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHEDEFGHFHHHHGHGHHHHHHHGHHHHHHHHFHGHHHHHGGGGGHHHGHHHHHHHHHHHGHHHHFHGHGHGGHGGGDCD.;FFGGGGGGGGFFFFFFFFFFFFF.>FFFFFF-BBEFFFFFBFFFFF-DDFFFFFFEFFFFFFFFFFFF?F///9FFFFF/;FFFFF?AADCFFEFAFFEFFFFFB/;B/A9 +@M01368:8:000000000-A3GHV:1:1104:7756:4776/2:R1:GGCCCATA:R2:CTACAAGC:F1:ACAC:F2:ATTA +TTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG ++ +GGGGGGGGFFHHGHHGHHGGHGHHGFHHGGGGGHHHHHHHHGFF3GGGHGGGHFDHEGHHFHHHHGFHBCGHGEGFFFFFHHHHHGHHHHHGHHHGGGGGHHGHHGGHHHGGGGGGHHECCFDHHADGGHHHFGFDC////FHHHDGFCGCDEECF?EGGFFHHFEHFGGHHFFF/A900;C.9..C0990.;CFBFGE/9.9B99/;B.99/FB///9AEF/99/9/BBF/B/B//A +@M01368:8:000000000-A3GHV:1:1105:11310:21761/2:R1:ATAAATAA:R2:ACTTGGGG:F1:TAGG:F2:GTAG +TAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCTCC ++ +GGGGGGGGGHHHHHHHHGGGGGHHHHHHHHGHHHHHHHHHGHHHHHHHHGHHHHHHHHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHGHHHDGHHHHHHHHHHHHHHHHHGHHHHHGGGGGGHHHHHGHHHHHHHHFFHHHHHCCAHFHHGGFFFFFFFFFAA;BDDFFFF00.-=D.ABDA-.::AAF;F@.AAD./0000.9-;.9:00009:F090;FF000900; +@M01368:8:000000000-A3GHV:1:1105:11578:14676/2:R1:ATTGAACG:R2:GCTCCGGG:F1:TAGG:F2:CCCA +AACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGT ++ +GGGGGGGGGHGGGGGHHHHHHHGHHHHGHHHHHFFFGGHGHHHHHHHHHHHHHHHHFGHHHHHHHGHHGHFHFGHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHDGGCGHHHHHHHHHHHHHFF<GGHHHHGFGHGEHGGA=A=DFFFFAFAFF?FFFFFFFFFADFAAD;FFFA:BBBAE.A.EBFFFFEF-..0BFFF9 +@M01368:8:000000000-A3GHV:1:1105:13575:19255/2:R1:TTAGTAAG:R2:GGTTCCTA:F1:TATG:F2:CTTC +GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTGGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTAACTATTACAG ++ +FDGFGFDGDEG1EGEFFBFHFGHA0E00A0CAAC0FFDFFAGHFEHHFDH/EAFCFFHACGHHAFFEF2DFFG/FGHHHHGHGH0C@/E@GGC0FG10>GGFGHHGHFFBGFGB2>F<EEF0@C@9:AAF00;9@-9.;FBBBBB->@;-A@FF<-;---@-;-----;-BFF/;---9//BBFFFF//B;FF///9BF///;/;B/BBFBFFBB/;-9--///;9/:-;BFFBBB9F +@M01368:8:000000000-A3GHV:1:1105:15502:11453/2:R1:GTCCTACA:R2:GATCACAG:F1:AGCA:F2:GTCT +TCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACCTGTCTCTTATACACATCTG ++ +GGGGGGGGGHHHHHHGHHHHHGGGGGFHFHHHHHHGHHHHHGHHHHHHGHFHGHGGGGGGGGHHHGGGGGGGGHHHGHGGGGGGGGGGGHHGGGFGGGFEGGGGFFFGGFGDGGGBFGFFFFFFFFFFFFFE0BFFFFFFFFFFFFFFFFFFF;BFFDFFFFBF0BFFBFFEEBFFAA009FFFFFBFFEBB0;.BFFBFFFFFFFFFFFF0;BFFFFE//090::B0000:009900 +@M01368:8:000000000-A3GHV:1:1105:16787:9771/2:R1:GTTATTAT:R2:TCCTACTT:F1:TATG:F2:CAGG +TCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG ++ +GGGGGGGGGGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHFHHHHHHHGGFGGHHEHHHHHAGHHGHBHHHGHHHHHHGHGHGHHHGGGGGHHHHHHHHHHHHHHHFFHHHHHGHGHGHHGGGACAFGGGGGG-AFGGFFFFFFFFFFFFFFFFFFFFF-9@AFEDFFFFFB.9-AABBBFFFEFFFFFFFFBFFFFFFEBB0BBBFFBFFBFBEDEAFFEB.ABFBFBFFFF0::/.- +@M01368:8:000000000-A3GHV:1:1105:17564:15723/2:R1:TATGCTCG:R2:ATCCGACA:F1:CCTG:F2:TCTG +TTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTT ++ +GGGGGGGGGHHHHGHHGHHHHHHGHHHHHHFHHHHGGGGHHGHFHHHHHHHFHHHHHHHGHGGHGGHGHHHHFHFHHGHHHHHHHHHHHHHEHGHHHHHGGGGGHHHHGEHHGHHHHHHGHFGHFHGGAHGGHGGGCCBDFGFFAFDBDDFFFFFBDA;AAD-ADC?AFEFB-@@AAEFEFFFFF..-:.B0:FFFFFFEF0BFF09FF.0;9BFFFFF0BFFFFFF?.::-909;.9 +@M01368:8:000000000-A3GHV:1:1105:18108:12091/2:R1:TAATAGGA:R2:GTATCCGA:F1:TGAG:F2:CATC +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTACTGTCTCTTATACAC ++ +GGGGGGGGGHHGHHHHFHHHHHHFHGHHHHFHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHGHHHHGHFHGHHGHHHHHHGHHHHHHGHHHHHGGGGGHHHHHHHHGHHHHHGFGHHHHHGHDHGHHGCA;?AAFGFEFDFFF.FFFFEBDFFFFFFFFBFFFFFFFFFEFEFFFEFFFFFFF.:BFFF0BFFFFFFFFFFFF;0FF0;BFFFFFFFFBFFFBFEFFFF0 +@M01368:8:000000000-A3GHV:1:1105:18331:16683/2:R1:TCTGGTTC:R2:TTATTATG:F1:CTAC:F2:TCCT +CAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGGGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATATCT ++ +GGGGGGGGGHHHHHHHHHHHHHGHHHHHFHGHHGHHHHHGGFGHHIIHHIHHHHHHHFHCFGHEEHFFHGHHFGHGHGHFHHGGGGGGEEGHHEHFHHGGHFHHFGGGGDFGBCGG?DGHGGFCGGHHECEF?CHGGDGFFGGHHC?EEGGHHHH.>AEGGGHEE-AEG0C0GCCEFFFFGEFG/;CEGGGE.:CAE.;@.@EFBFFFF/..:BFF0:BB00FBFFFFBDAFFF0;0B +@M01368:8:000000000-A3GHV:1:1105:20577:18421/2:R1:GGGGGTAG:R2:ATGTCCTA:F1:CTAA:F2:CAAG +ATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGCCGAAAATACCAAATGCATGGAGAGCTCCCGTGAATGGGTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTT ++ +CFFGGGFGGHHHHHHHCEGHHCHFGHCAFEFHHHGGAEGGHHDHHHBGHBGHHH?5BGE1AEEFHHHHHGHFHBGHGEGGGGHBFHHFGHBGCFFG4EGHGG?E/FG3F3F/FGCDECEA/FGG/CEDBB//?BHEHHF0ADC@<AFH1CDDGCGG..<C--:D000CF0::C009B9F/B..909C9....009.;;/900:A;;A////:BFFF/BFBFFFF.9.//;999//;9/ +@M01368:8:000000000-A3GHV:1:1105:21099:2895/2:R1:TAGCTAAA:R2:TTATTATT:F1:GTGA:F2:ATGT +CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATCGACCTGTGATCCATCGTGAGG ++ +GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHFHFHHHHHHHHDEGGGHHIIHHIHEGDFHFC1EAFHFFHEGHHGHFHHGHFHFFFFGGGGHGHFFGFHEGHHGHHHHHGGGGGHECFGADHFHGGCGGEGHG/EGFHHGGDGHFHFFCECC@E?F1FH?ECGGGGHFCAEAFFHBC0CACF0BFFGGGGGFB:BEFEDGD0::;9FBB0:BF9@=...;9-AB;B0;F00:;.9:/.. +@M01368:8:000000000-A3GHV:1:1105:26469:19245/2:R1:TTATGATT:R2:GGGGTAGC:F1:ATGT:F2:TAAA +TGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTTGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCT ++ +GGGGGGGGGHHHGGGGGHHFFGHHHHFHHHFFHFHHHFHHHHHFGFHHHHHGHHHHGGGGHHHGHHFHHHHHHHGHHHHHHHGGHGGHHHHHHHHHHHGHHHHHHHHHHGHHHHGHGHHGGCGGGHHHH23FFHHHHGHGCGHHHHGGHFHGHBCCFFFFFFF/ECCDFDFEFFFFBDAFF?DADE??BFFFF=A-.AAAA.BFFFFED..BF/BFFEFFFFBFFFFFFFFFFBFB:: +@M01368:8:000000000-A3GHV:1:1105:7169:17145/2:R1:AACCTAAA:R2:CATTCAAT:F1:TAGC:F2:TGTT +TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGGTAATAGGGGGATAGACCTGTGATC ++ +GGGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHHHFHHHHHHHGGGGHHHHHHHHBGHHHHGFDGFHGGHGGHHHHHHHHHGHHHHGGGGGHGFHHHEHHHGFGHHHHGGGGGGHEHH3FGHHHGGGGGGHHECEGDFGFCGDHHHHFGGGC?@?CDGHGGGGC-:.ECDGDGGGGGF0.00BFGGGF/BD.00FG..;9.9;E.;BFFFFFF.9-.99///9BB///; +@M01368:8:000000000-A3GHV:1:1106:15481:16176/2:R1:CTTTAGTA:R2:AAAGTGAA:F1:AGTA:F2:CTGT +TCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTAT ++ +GGGGGGFGGHHHHHHHHFHHHFHHHHGHHHHHHHHGHHHGHHEGHHHGDGCHHHHHHHHHGEHEHHHHHHHFHGGEGGHHHHHHHGEGHGHHDHHHHHHHHEHFHGHGHHHGEGCEHHFHHHGHHHHHHHF?GHHHFEFFAFFHHGG?;@DDFFB?@B;BDAFFFEBBD>D>D9@DDCFFFFFFCFDFE.AA;/BB/.;-.;BFBBBFFBFFFFFFF/9FF?/;/BBFFFFF/;FFB/ +@M01368:8:000000000-A3GHV:1:1106:17389:18671/2:R1:CCATAACA:R2:GTATGTTC:F1:CTTG:F2:GCCT +TAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTCTTTAGGGTTTATGACCCTGA ++ +GGGGGGGGGHGHGHHGFGFGFHGGHHGHHHHHHHHFHHGGGGAGFBGHHHFBBGH5GDHH5EEEGEHEGFBF1DGFDGFGGGBBCEEGEHFEFDDHFBFHFD/@BGDGDHHGDCCFCD/C<->.ADDGE1GA<DBGFGHBG/..DGFCDGD.:0:.;CFGHEHBF.A-99CFGGFF0C0C0BF0;CA9/B:///;//BF/9B9?-..:;.;9.9A././;B//F.B?A////9;:/:/ +@M01368:8:000000000-A3GHV:1:1106:22927:3863/2:R1:GTATCCGA:R2:AAGACAGA:F1:CATC:F2:TACT +CGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGG ++ +GGGGGFGGGHHGGHHHGGGGGGHGHGDFGHFGGGGFHHHHHGGGGGGGGFHGDFGGGGHGG?@EFHGEFEHEGFHHHHHF2FEHHGHGDCEAD?GFDFGGHBGHHFEHHHHHHHHHDFGHGHHGBDFG0FFHFGGFHHH=GGBF-AACEHEHHGGGHHHGHHFHCCFEBB0FFFFFF/;0;;FFFBFE.AA0BF0??-AD/////;:BFFBFFBBBFFFFFB/.9ADDAD-D;-;=A- +@M01368:8:000000000-A3GHV:1:1106:23625:4236/2:R1:TAGCTAAA:R2:CACTTTAG:F1:GTGA:F2:TAAG +ATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGGTGGGCTATTTAGGTTT ++ +GGGGGGGGGGHHHHHHHHGHGGHHHHGFGGG?HGGHHHHHHHGGHGFGHGEEGFHHHHHHHHHHHFHHFHHGGCGGGHHHHBFGFFHGGGEFGHHGFFEEFHGGDGEHFHHHGGGCCCGHHG?EGFGGGFED@D?CEDFHEHGHFFHFHHGCGG.:CGGD:AEDCG?C?BFFFGGG/CEFBFEFFF/;:BBB;CFGD.:/:B:BB/BFFFBBFBFFAA9A..9-9@:/./;B/9/:/; +@M01368:8:000000000-A3GHV:1:1106:26031:14826/2:R1:GATCACAG:R2:CTGTAATA:F1:GTCT:F2:TTGA +CGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGACCTAGTGGTGATATAGGGGTGTCCCGCGTT ++ +GGGGGGFGGGGHGGHHHHHHHGFFHHCHGGGGGHHHHHHFHFFGFGHHHHHG0EEEHHGHFFGFFHGGGCFGEFHGGGGGHGFEEGEFHGHFFGFGGGGFGDGGGG?DCCCCCDDFGHHFHCGHGHHGHHFBAFGGHE.<DCDD<CD;GFFEFHH.@?A//B0F0/;09CFFBBCF0;;F;B0;009FBFFB9.9ADDFFFFAACAC;-9;BB/B././9//;//...-;///---;9 +@M01368:8:000000000-A3GHV:1:1106:27607:15990/2:R1:GACAGATA:R2:CCCCTTAA:F1:CTGC:F2:ATAA +ACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTTGAGAACTCGGGGGTTGCCCTGTCATTCACAAACAAACACATAGCACTCAAG ++ +GGGGGGGGGHGGHHHGHHHHGHHFHHHHHHGHHHHHHHHGGHHHHGGGGFHHHHHHGHHHHHHHHFGHHHHHCHGHGHHGGGGGGGHGFFGG@CEDGHHFHHCFGBDDGGG?AFGFGGGGAEAFDFFFFFFFECFAFFFFFFFFFFFFFFFFFFFBFFEFFFFFFFFFFDAD;=DADFF-.A.BB0000;.00;:..-;----.000.;000;00009...;A.A..0000000//00 +@M01368:8:000000000-A3GHV:1:1106:6455:17124/2:R1:GTATCCGA:R2:GCATTAAT:F1:CATC:F2:TAAT +AACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGG ++ +GGGGGGGGGHGHHHHHDGGHHHHGGGGHHHHHHHHFFHFHHHFGFGHGGHGGHHHHHFHHHGGGHHGGGGGHFFHHFHHGHHHFHHHHHGGCEGHGHHHGGHFGGGGGGGHHFGGC/CGGGADHHHHFGGGG?DCFHHHFGFGGCEHECACDGEHHFCGH0CFHHHHHFHFEBFFED.EED0CE0C0CFGGGGGA.;CBBBFBF/BFFFFFFEA;FFFF/BBFF//BBFBFFD.:9A. +@M01368:8:000000000-A3GHV:1:1107:14451:26748/2:R1:GTATGTTC:R2:AGCTAAAG:F1:GCCT:F2:TGAA +TGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT ++ +GGGGGGGGGGGGHHHHHGGHHHHHHHHHHHGHHHHHHHGGHHHHGHHFHHHGGGGHHHHHHHHHHGHHHHHGHHHFHGGHGGFHHHHHFGFGFHHHHHHHHHHHHFHHHFFHHHHGGGGGGGFFHHGGHHHHHHHFHHHHHGGFDHGHGGGGBFFFFFBAD@>;9.FFFFFFD-99ADFFD-.9FADF--9..;AAFEFFEAA-;.///BB:BBF/BBB//B//99/;BBB/9////F +@M01368:8:000000000-A3GHV:1:1107:18714:24788/2:R1:GTATGTTC:R2:GGCCCATA:F1:GCCT:F2:ACAC +TGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTAGGTCGCAGGAACTGTCCTTGT ++ +GGGGGGEGGHHHHGGFFHHHHHHHHHHGGGGGHHHHGHHHHHHHHHHHHHFHHHHHHHHGHHHHHHGHHHHGGCGHHHHHHHHHHHHHHHGHHHHHGDGHGGHHHHHHHHGHHHHHHHHHHHGHHHHHHEHHHGHFGGGGHHHHEHFHHGHFHHF.>GGHHHGHGHGGHCFFCDFDFFFFFFAAF.FFFFFEFAFCFFA..@.AFFFC9--9;.;A///00--;A-.;.000000090 +@M01368:8:000000000-A3GHV:1:1107:21355:8333/2:R1:ATATTGAA:R2:GTATCCGA:F1:CGTA:F2:CATC +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCGGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +GGGGGGGGGHHHGHHHFHHHHHHGHGHHHHHHGHHHHGGGGHHHHHHHHHFFHHFHGHHHHHHGGHGGHHHGHHHHGGHFHHHHHHHFHHGHHGEFFHFHHGGGGGGHHHFHHHHGHHHHE?FGHHHHGFGHGHHGGGDGGGGGFFFCFFFFFFFFFFFFA;DBBDFFFAFFFF;DBAFEDFFFFFEA?-9...9;9;BFFFF//BFBFFFFBFBFFFFFB/BFFF9B/EDEF-BFF. +@M01368:8:000000000-A3GHV:1:1107:22864:15712/2:R1:AATAAGAC:R2:GTAATATT:F1:ATCA:F2:GAAC +TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTCTGTCTCTTATACACATCTGACGCTGCCGCCGAGGGCTCTAGTTGTGGTCTG ++ +GGGGGGGGGGGGHHHHHHHHHHGHHHGGGGFHGHHHHGHFGFFFFGGHGGGGGCHFHFAFEDHHGGFGGGHHEGEGGHHEGGCHGHHHHGGGGGGHGF0EECGGGGFECCFFGFBGHHHHHHGGHHGHFCFEHFBEFGGADDDC=CC0CCFHG:CEHHHGHBGH:GFFGFF0CAAA/BF09BBBBBBB9CFFB0C00;000;:CFFB;:...D?>:@=:9-@...//;////..;/;/ +@M01368:8:000000000-A3GHV:1:1107:24817:14233/2:R1:CTTCAGGG:R2:CTTTAGTA:F1:TCAT:F2:AGTA +GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAAAGTGTGGGCTATTTAGGTTTTT ++ +GGGGGGGGGFHHHHHHHHHHHHHHGHHHGEHGGHHGHHHHHGHHEHHGGGGEHGHHHGEHHGHHDGHHHHGGGGGFGEFGGEGHHGCEEEGHHEFGGDHGGGGAGFHHHHGGF/?//GHFFDGGGGGHAD??DC1DHHBGHHHHGHF<<//ACFHHCDCDE?CGEBEGFFFGF?.?DGGGGEFEFFB00CFFFB.:AFFB;/BB9BBFFBBFFFAD.;///9.A./A:FB;BF//B.. +@M01368:8:000000000-A3GHV:1:1107:24830:14440/2:R1:AACTGTAT:R2:CTTTAGTA:F1:CCGA:F2:AGTA +GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTAGTTAGGTTTTT ++ +DGGGGGGGGHHHHHHHFGHHHHHHGHHHGGHGGHHHHHHGHHHFHHHGGGGGHHHHHGHHHHHHHHHHHHGGGGGHGHHFGGHHHGGGCGGGHHGGG?GGFGGGHHFHGH?FG/CDHHFAAEGGG/DHFC?-.AFGFH0CHHFH0DHHG/CDEBFHG.CA.:0C:;.FGFEFFG:E.B909BFF/;CFGBB9BBA.:CFGFFFFFFFF/;//AF?.ADEAB.9.A/;.:BFFF/9FF. +@M01368:8:000000000-A3GHV:1:1107:26478:23602/2:R1:GCTAAAGT:R2:ATCAAAGA:F1:GAAC:F2:CAGA +ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGCGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTAGCCTGTCTCTTATACACATCTG ++ +GGGGGGGGGGGFHHEFGHHHGGGGGGHHHCGGEFHFGGGFHHHHHGGGEGGHHFHEEGEG/EEEE/?/EHFHH3GGH4EGHHCB01CGHGHGD//<@CGGBFFDGHHHH0FEHHGEGFHCD11>0=GGHGCCFGHGBGGD0GGHC0C.@?ACEEC/.EDEB?BFFCFB9CFF090BBCBFBF0CCB0B00A.9E.B/9BA=-..B9FBF//;BBBBFFB:./9/9/9;B//9BF/;B/ +@M01368:8:000000000-A3GHV:1:1108:11059:23181/2:R1:GTTATTAT:R2:AACTGTAT:F1:TATG:F2:CCGA +ATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC ++ +GGGGGGGGGHHHHHHHHHHHGHHHHHHHHGHHHHHHHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHHHHHHHHHHHHHGHGHEHHGFCGGGHHHHHHHHHHHHHHHFGHHHHHGHGHGHHGGGBFGFFFFEAFFFA.BFFFEFDDFFFFFFFFFFEEFF;D;EFFFFFF0BFFFADDFFFFFBFBFFFFFBB0FFFF0::0BF000BFFFBBF0EDFF@ +@M01368:8:000000000-A3GHV:1:1108:12233:13498/2:R1:TAGGTGCG:R2:GGTTCCTA:F1:ATAA:F2:CTTC +GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACTGTCTCTTATACAC ++ +GGGGGGGGGHHHGHHHHHHBGHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHGHHHHHGGHHGHHHHHHGHHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHHHHGHGHGGHGGGGAGGFGGGGGGGGFFFFFFFFFFDFFFFDFFFFFFFFF>.AEDEFFFFF?E-DAB9FFFFFFFEF:FBFB009:0FFFF9BFFBFFFFBB0.9A=-/0BFF0BBFFBFF:90: +@M01368:8:000000000-A3GHV:1:1108:14328:14751/2:R1:ATAATAGG:R2:GTATCCGA:F1:ATGA:F2:CATC +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATCTGTCTCTTATACA ++ +GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDHHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGHGGHGGHHHHHGHHHHHHHHHHHHHHHHFHGHHHHHGGGGGHHHHHGHGHHHHHHG?FHHHFHGHGHGHHGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFAFFE.ADEFFFFFFFADFFFFF///BBFF/FFFFFBFAFFFFBB///;9:BBFFFBF9B9//:/ +@M01368:8:000000000-A3GHV:1:1108:16350:22621/2:R1:TTAGTAAG:R2:CATCTGGT:F1:TATG:F2:TCCT +CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATAT ++ +GGGGCGGGGHHHHHHHHGHHHHGHGHHHHGGGGHHHHHHHHHHHIHHHGHHHHHHGGHGGHHHHGHGHGHHHHHHHHHHHGHHHHHHGGHHHHGGGGGHHHHHHHHHHHHHGHGHHHGHHGGGGGHHGGGDADDGFFGGGGFFFFFFFFFDFFFDFFFFFFFFFFFAFAEFEDFFFFFFEE-AAFFFFFBFFFFF0FFFBFF;AFFFFFFFFFFFFFFFFFFFFFAB9BFFFFFBFFF +@M01368:8:000000000-A3GHV:1:1108:21791:23272/2:R1:CTTTAGTA:R2:CATAACAC:F1:AGTA:F2:TTGG +GGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCT ++ +GGGGGGGGGHHHHGGHHHHHHHGGGEGGHHFGDHGHHHHHHGGHHH2GGHHHGHHGGGGFGFBFHHHGGGGHGHGHHHHHHHHHHGHGHHGHHGGHGGHHHHGGFHGGGHFHGHHHHHGGHHHGHGFCGFFDGGG/FCFHBGHHHHHHHGHCHHHHGGGHGHGHGCFF;>@DFFBFFFFFFDFFFFFFFF;;.DDAA;FFF;BB;C@..9::BBFBBA;-:.::FBB///;/////:/ +@M01368:8:000000000-A3GHV:1:1108:23314:26089/2:R1:ATTGAACG:R2:CATAACAC:F1:TAGG:F2:TTGG +GGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCC ++ +GGGGGGGGGHHGDHHHHHFHHHHGGGGGFHFFGFFHEGHHHHHHHHFGHFHHHHDEHHHHHHGHFHHGGGGHHGFHHHHHHHHHHHHHHHHHHF?EEFCHFGFHHEFFFHGFHHGHFHFHHHFFHAG??GHACGGDGHGGBFHHHHHHHGHFGHGHGEGHGCFFF@CD-BDDFFFFFFFFDDFFBBFFD-B-A.DFFA.AFEFF-;-..:..BFFFFFA-../9FFF//B/BBBBFF/ +@M01368:8:000000000-A3GHV:1:1108:25084:18127/2:R1:TTATTATG:R2:CCCTTAAA:F1:TCCT:F2:TAAG +CATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAG ++ +GGGGGGGGGGGHHHHHHHHFHHHHHHHHHHHHHHGGHHGHHHHHGGGGFHHHHHFHHHHHHHHHGGHHHHHCGGHGHHGGGGGGGHHGGGGGGGGHHHGHHGGGGGGGGGGGGGGGG@CDE?EFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFE;DFBFFFFFFFFFFFFFFFFFFFAFFFFFFFBFFE0BBBFFFFFFFFFFBFFFF9BB0:0:B; +@M01368:8:000000000-A3GHV:1:1108:7626:10048/2:R1:ATCAAAGA:R2:AACCTAAA:F1:CAGA:F2:TAGC +CACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGATCTCTGGGGTCGGCGGATTT ++ +GGGGGGGGGHHHHHHHHHHHHHHHHHHHGGGGGHHHHHHHHFGHHHHHHHHHHHHGHHHHGGFHHHFGGGGHHHGHHHHHHHHHHHFGHHHHHGHGHGHHGCGCGGFHHHGGGGGGGGGGGGGGGGGGGFFFAFFFFFFAA-AFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBBFFDFFFFFB-<>;>@BE<:000.0.0/0<00<0:>..-<<<---.0 +@M01368:8:000000000-A3GHV:1:1109:11270:12255/2:R1:AATCAAAG:R2:CTACCTCA:F1:ACAG:F2:GGGT +ATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGATT ++ +GGGGGGGGGHHHHHFHHHHGGGGHHHHHHHHHHHHHHHHHHHHGHGGHGGHHGHHHHHGHHHHHHHHHFHHHHHGHHGHHHHHGGGGGHHHHHHHHGHHHHHH?FHHHHHGHGHGHHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFECADFFFF.DEBFFFFFF;D.B<FFFBFFFFFFFFFFFF0FBFFFFFFFFFFFFFFFBDDDFEFDFF@--@-9.<:000.0<0<0 +@M01368:8:000000000-A3GHV:1:1109:19224:11655/2:R1:AAATAGCC:R2:CCTGTAAT:F1:CACA:F2:ATTG +ACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTCTGTCTCTTATACACATCTGAGCTTC ++ +GGGFGGGGGHGGHGGGHHHHHHHHHHHHHGGGGGHFHHHHHHFHHHHHFHHHGGGGGHEFHCEEHHHGGGGFGGGFGGGGHEGGGEHFHHHHDGGF?AGFHGGGGGGGGHGGGGGGGHHHHHHGFFFHHHHFCEFHFHGF<EGFHFGGAGBFHHHG/C.;C:0:GGG00CFGEFGBA.9C:BBFFGFGGFFFBFGAGG.D..:A.DFFEFFFBFFF0099:9BB0BFB009000;000 +@M01368:8:000000000-A3GHV:1:1109:21978:9490/2:R1:GGGTCATA:R2:GAATCAAA:F1:AAAC:F2:GACA +ATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGGTCCAGTGTAGAGTTATGGGG ++ +GGGGGGGGGGGGHHHGHGGHHHGGGGGGGFHGGGGHHGGGGHHHHHHGGGGG?HHHGGGGGG?E1FEEE?EHHHHFHAGHHHHHHHHHFHHHHGGFDEG2FCFFGHHHHFH?FF<FFGDHHHHBGHHEGFDD<GFGFDBGHGGHH0GDFH@<AEE.::CFGGHGGBGFGG0BFAF0000BFDEGGFGGGGGFGE00BC;;B00.;AAD.;--------.-;/:9////////////;9 +@M01368:8:000000000-A3GHV:1:1109:22910:4079/2:R1:TAATAGGA:R2:CTCCGGGC:F1:TGAG:F2:CCAT +ACACTTGGGGTAGCTAAAGTGGACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCT ++ +GGGGGGGGGGEGGHHHHHHGHHHHGHHHHHHGGGFGHGFHHHHHHHHHHHHHHHFHGHHHHHGGHHHHHHBGHHGGCGGHEHHHFHHHHFHHFHHHHHHGHGGHGGHHHHHHHHHGHHFFHHHHHGHHHHHHHGHHGHHGG?FGHHGHHHHHHHHHHHGGHHHHHH?CEHGFHGGF--@DFFFFFAA;DDFFFFFF?@FC.A=DD-.AFFFF;->;.ED.FFFFFFF-.:00009BBF +@M01368:8:000000000-A3GHV:1:1109:24217:19077/2:R1:AAAGTGAA:R2:TAATAGGA:F1:CTGT:F2:TGAG +CAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGAACCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTTCTGTC ++ +FEGGCFGFGHHHHFHHFHHHHHHGGEEEFGHGFFAGFFEEAEGGHHBEEFAFGAEF1G3GFGGEEF@EAGGHGFF?ECGGHEEEFGGHHHHFHHFHHGFHHHC2D<FFFFAFDFF<FF1FGGBHHFF0>AGHGG00<G<<DGBGDD0<.;CGG;;:;CFFHHHHHHG?D.;.;/ACD.;FFFB0CFBFGGFGGB0;EFAFF9B/:BB/;;AEFF//:AF-A.;//:B///;BFBB:;/ +@M01368:8:000000000-A3GHV:1:1109:25237:11722/2:R1:CCTGTAAT:R2:CATCACGA:F1:ATTG:F2:TGGA +CACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTCGATCTCTGTG ++ +GGGGGGGGGHHHHHHHHHHHHHHGHGHHHHHGGGGGHHHHHHHHHHHGHHHAGHHHHHGHGHGHGGGGCGF?FHGGGGGGGGHHHGHHGGGFFGGGGGGGGGGGGGGGE?DEGGGFFEF;DDFFFFFFFEFFFEFFFFFFE./B9BFFFBFFFFFFFFFFFF>-BFFA9FFFFFFFFFFFBFAFFAFFFFFBFFFFFFF/9BFFDFFDDFF-9@CDAD;:AFF/;B//....;;///; +@M01368:8:000000000-A3GHV:1:1109:27103:7153/2:R1:GTAATATT:R2:NTACTTCA:F1:GAAC:F2:GGGT +NTAAAACCTNAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCANGGNAGCTCTCCNNGNNNNTGGTATTNTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTC ++ +#AAFFGGGG#BAFFAGHHHGGGGHGHHHHFHHHHHHHGHHHHHHHEGFGGHHBGHGHHBGFFEG2GHHFHFHHHGHHGHFH#/B#??FFGHHH##?####????FHG#??CFGGGEF>CGAGAGGGFGAA@@FFFFFFFFB=@@FF?FF;FFFF@@-@-AFEF?BB/BF?E-BBFFF;:BB/BFFFB/BFFFBFBFBBFFFFFB/FFFBB/;9A@@BFFBFFFF/BBFFFFFFE/:9/ +@M01368:8:000000000-A3GHV:1:1109:8763:7859/2:R1:TAGTAAGT:R2:GGTTCCTA:F1:ATGT:F2:CTTC +GGGTCATAAAGCCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG ++ +GGGGGGGGGHHHHHHHCHFAFHHDGEGFHHHHHHHHFHHHHFHHHHHHGHGGHGGHHHFHGHHHHHFFHHHHHHBGHFFHGHGHHHHHGGEGGHHHHHGHHHHHHHHHGFGHHHHGHGHGHHCCGGGGGGGGGGAAGF?FFFFBFA@=BDDFF?-;FFF;@CFFEFEFFFFFFFFFBFDFFFFFFFFFFFFFFFFFFFFFF:FFFF/BF/FFFEFF?DECAFEFFFFFE:FFFFFFFB +@M01368:8:000000000-A3GHV:1:1110:12808:18367/2:R1:AATTAACA:R2:GCCCACAC:F1:CACT:F2:GTTC +CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACAGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCTT ++ +GGGGGGGGGHHHHHHHHHGGGGGGHHHHFHHGHHHHHHHHHHHHHGHHHGGHHHH3GFHEFHHHHHGHHGFHHHHHFGHHHHHGHGHGGHGGGGGGGHHHGGCGGGGHHGGGGGGGGGGGGGGGGGGGF-BAEFEBEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFAFEFDFFFFFFFFFF0BFBED=@DFFFFFFFFFEFFFFFBFAFB;0:;F0 +@M01368:8:000000000-A3GHV:1:1110:14751:26540/2:R1:TAACACTT:R2:ATGTTCGC:F1:GGGG:F2:CTGT +ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGGTTGGGCTATTTGGGTTTTTTGACCCTGAAG ++ +GGGGGGGGGHHHHGGFHGGHGGCHHHHHHGHHHGHHGEGGGHHHHHHHHHHEFHHHHHHGEE?FGHGEHGGHHHGGGGGGEHHGGGGHHGGGEGHHHGHGGGDFGHHGHGGGGGCGAFFCCDFHHHH1CGGHBHFHFCGFFCDFHGCCE.CC:EGFHHCGHHGCF?FGGGFGGG;B9;FFFFFFEGEBF0;;;0;CFBFFF?D.DE.:..99-...;;//9A.;DE.9-./:FB.;// +@M01368:8:000000000-A3GHV:1:1110:15519:10008/2:R1:CTGTAATA:R2:GGTTCCTA:F1:TTGA:F2:CTTC +GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAG ++ +GGGGGGGGGHHHGHHHFHHBGHHHGGGGHHHHHHGHHHHHHHHHHHHHHHGGHGGHHGHHHGGBGHHHHHHHHHHHHHHHHHGGFHHHGGGEGHHHHHHHHHHHHHHHGHHHHHHGHGHGHHGGGGGGDGGGGGGGGGBFFFFFFFFFFFFFFFFFFFECB-=BFFFFFFFFBB?E-A::FFBFFFFFBFFFFFFFFFFFFBFFEFFF;0BFF0:FE?FFFFBFFFFFFFFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1110:16559:7507/2:R1:CATTCAAT:R2:GCCCACAC:F1:TGTT:F2:GTTC +CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTAAT ++ +GGGGGGGGGHHHHHHHHHGGHGGHGHFHHHHFHHHHHHHHHHFHHGHHHHGHHHHHGGEEGHHHHHHHHHHHHHHHHHHHHHHGHGHGHHGGGGGGGHHGGGFGAGFGGGGGGGGGGGDDDFAGGFFFFCFAFEEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFE..@BFFBD./;:0FFFFF09B/AFCA=-99BFFBFFFFFFFB:BFBFFBFB9: +@M01368:8:000000000-A3GHV:1:1110:16700:6843/2:R1:AAATAATA:R2:GTTCCTAC:F1:GGAT:F2:TTCA +GGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTCTGTCTCTTATACACATTTGTCGCTC ++ +GGGGGGGGGHHGHHHGGHFHHHGGGGGHHHHHHHHHHGHHHHHHHHHHHGGHGGHHHHHHHGBGFFHHHHHHGHHHHHHHHGHHHHHGGGGGHHHHHHHHHHHHHHHFHHHHGHCG/GGHHGGGGGGAFGEEADAGGDFFFF00D>CFDDFFFB?AF?B;-@>.FEDFFFFF0BF-BDFFFFE00B0BF0FFFF:BFA0B0:BBFBBFFFFF0B0:000<00<BFBB0<00000<99. +@M01368:8:000000000-A3GHV:1:1110:18014:7856/2:R1:ATAAATAA:R2:CATCACGA:F1:TAGG:F2:TGGA +CACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGAGCTCGGGGGTCGCCGTGTATTTTTACAAAAAA ++ +GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHGGGGGHHHHHHHHHGHHHHHEGHHHHHEHGHGHHGGGCCGGHGHGCGGGGDHHHHFHGGGGGGGGGGGFGGFGAC-EFEGGGFFFGFF;DFFFBFFFFFFFFFFFFFFFFFFFEFFFBFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFB?DFF---;--D;9AFB0B:B0::00;0.;----.--.-9-:/00::090:0;..: +@M01368:8:000000000-A3GHV:1:1110:23303:11846/2:R1:ACTTGGGG:R2:GTCCTACA:F1:GTAG:F2:AGCA +TAATTAATTAACACACTTTAGTAAGTAAGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAA ++ +GGGGGGGGGHHHHHHGGHHHHHHHHGH5GHHHGGGGHHHHHFHHHHHHHEHHGFBGEEFE1FG5GFHHHHHFHHHGGGGGHHHHHHHHHHHGGHHHHHGGGGFHHFHH/EHHHGG?@EFHHHGGGGHFGGGGHHFHGGDGGGGFFFHGFDGGGCGACC;DFFB0CH0CCCFGBFBFB/:A?FFFE?AA.CFBECE0BBBF0F/A./9FFFFF./;/FFFFFFA.:////9BB/B/BB/ +@M01368:8:000000000-A3GHV:1:1111:13195:14562/2:R1:ATCAAAGA:R2:GTATCCGA:F1:CAGA:F2:CATC +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACATCTGACGCTGCCGACGG ++ +GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHDGHHHGGGGHHHHHHHHHHHHGHHHHHHHHHGGGGGHHGHHHHGFGFHHHHHHHHGHHHHHHHGHHHHHGGGGGHHHHGHHHHHHHHHHGFHHHHHGFGHGHHGGGGFCGFGFFFFAFFFFFFFFFFA;BADFFFAFFFFFFFFFEFFFEFFFFFFF;FDBFFFFFFFFFFFFFFFFF/BFF/BFFFEBFFFEFFFFFFFFF@B@- +@M01368:8:000000000-A3GHV:1:1111:14287:20817/2:R1:GCCAACAC:R2:ATTCAATT:F1:GTTC:F2:GTTA +TATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATGAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGTTAGACCTGTGATCC ++ +GGGGGGGGGHHHHHHHHHHHHHHHHHHHHHGHHHGHHHHGHHHGHGHFHHHGGGGHHHHHHHHBGGGFHHFGGHHGGHGHHHHHHHHEGFFHHFGGGGGHHEHHHBHHHHHFFHHGHGGGGGHFGF3FDGHHGCGGFGHHGGGG/2GG@E?GHHHGFEDGGC<FGHH?EGGG@DH.@ECEGGGGGGFGFFB0FGGGGGGEGGGFGEFEF0BE0AFFBFFFF9.-.00;000;;90;99 +@M01368:8:000000000-A3GHV:1:1111:23624:14516/2:R1:GTCCTACA:R2:CGACATCT:F1:AGCA:F2:GGTT +CTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACCCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAA ++ +GGGGGGGGGHHCGHHHHHHHGHHHHHHHHHHHGDGEHHHHHHGHHHHHHHHHHHHHHHGGEFGHHHHHHHHBGFHHHHHHHHHHHHHHGHGGGGCEFGGCGHHHHFHHHHGHHHHFGHHHHHHGHGHGHHBCA?B-9FGFG?D;BD?9BFFFFFFF;DFFFF-.A;ADF-@=EF??A.BFFFEF>FDFFFFFFFFFFFBFFFFFFFFFFF/FFF/BFFFFFFFBA.?@;FFBDFFFF/ +@M01368:8:000000000-A3GHV:1:1111:7437:24508/2:R1:ACACACTT:R2:ACATCACG:F1:TAGT:F2:ATGG +TCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTCTGTCTCTTATACACATCTGACGCTGCCGAGA ++ +GGGGGGGFGHHHHHHHHHHHHHGHHHGFHHHHGGGGDHHHHHHHHGFHHHHHGHHHHHHGHFHGHHGGGDFGDFFGGGFFGFFGHGHHHGGC?AD-:A-AFFGGG-?9EFEFGGFFBFEFCFFFFFFFBBBFFFFFFFFFFFFFFFBBFFFFFFFFFFFFFFFFFFEFFFFFFBFFFFEFEFFFF;CF-FFFFFFFF/BFFFFFFFFFFFFEFBFFFB/FFFFFFF//9A..;F--@9 +@M01368:8:000000000-A3GHV:1:1111:8665:25542/2:R1:TCCTACTT:R2:CTTTAGTA:F1:CAGG:F2:AGTA +GTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGACAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTA ++ +GGGGGGGGGHHHHHHHFHHHHCHHGDGGGGHGGHHHHHHHHGHGHHHGGGGGHFHHHHFHHHFFHHHHHFGCGGEHFHHFGGHFHGGGGGGHHFEEEAFEFFDFHGHHHHGGGGGGGHH0DGGGGCGHCC-<CCBGHHHGEFFFFHH.C/<AEGHHEG.EEA0GE0:FB0CFGF:A?FFBF??FG0B;FGGGGFGE?BBF;BFBFFFF/F/B?DDF.;.;:ADD.BA/BBFFB:BFE/ +@M01368:8:000000000-A3GHV:1:1111:9655:13916/2:R1:GGCCCATA:R2:TTAGTAAG:F1:ACAC:F2:TATG +TCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATTGCGTGGACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +GGGGGGGGGHHHHHGHHHHGHHGGHHGGHGGHHHGHHHHFHHGHHAFGGGHHHHHGHHHHFHFHHHHHECGGGHGHHHEFHFHGGGGGGGGFGFFGFFEFGEGHHHGHHGGDGGGHGFGGGGGGGGE@GCGGHHHGHHHHHGHHHHFHHGEGFFEGCE.ECCECEHCGGFFE/AABFFG0CFE0BFFF9CFGG.F/BBBFFGFGGGFFFFFFBFEDAEFFFFFBFFFFFFF0;FEBF: +@M01368:8:000000000-A3GHV:1:1112:10272:7828/2:R1:GTATCCGA:R2:GTGCTCCG:F1:CATC:F2:GCTC +AGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCAAGTGTAGTGCTCGGGGGGG ++ +GGGGGGGGGGGGGHHHHHHGGGGGGHHFHGGGGGFGHGGGGGGHHHHHHHHHHFHHHHFHGFFHHGF?ECEFFFFFFHHHHHH?FHHHHHHHHHGFHHHHHHGGGFGHHHHFHHHHHHHHHHGGGFGFGCGGGGHHHHHHHHHHHGGFFHGHHHHHFFHFCGFHCCGHGHCGGHG?DGGGGGFBBFGFBFBBBGGGB0000;CD?DFFAFF9=--;...://;////////.------ +@M01368:8:000000000-A3GHV:1:1112:9832:16531/2:R1:CTGTAATA:R2:GTATCCGA:F1:TTGA:F2:CATC +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +GGGGGGGGGHFHHHHHHHHHHHHHHGHHHHGHFHHHGEEGGHHHGHHHHHHHHHHHHEHGHHGGGHGGHGHHHHHGHHHHGHHHHHHHHHHHHHHGHHHHHGEGCGHHHHHGHHHHHHHHHHHHHHGHFFGHGHHGGGDACDFFFFABFFFFFFFFFFD-BC?DA9.DFFBEA==B=?F.;ABBFBFEF-DAF/BFF9/FFFBB/9BFFFFFFF/BFBBFFFFFFF/9FEA?--B/;. +@M01368:8:000000000-A3GHV:1:1112:9832:6701/2:R1:CGCCTGTA:R2:GCTAAAGT:F1:ATAT:F2:GAAC +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATT ++ +GGGGGGGGGFGHHHHHGHHHHHHHHHHHHCGHHHHHHHGHHHHHHDGHHGGAECHHGHHHHHHHGHHHGHGFHHHHGGHGGHHFHHHHHHFHHHHHHHHH2GHHHHGHGHHFHHGGFGGGHHGHHHHHFHHHHEFHHHHEFGFFHGEHCFGDFDFFFFFCFFFFFFFFFFFFFAFBFFDFFFFFFFFADCF?9?EFBFB9EF-A.;BFFFFFFFBFFFFFFFFFFFFBFFFFFFFFFF +@M01368:8:000000000-A3GHV:1:1113:13532:8618/2:R1:GGAATCAA:R2:GCCCATAA:F1:AGAC:F2:CACT +GGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTGGAT ++ +GGGGGGGGGHHHHHGGHHHGHHHGHHGGGGEHHGGHHHHHHHHHHHHHHGHHGHHHHHGGHHHHHFHHHHGCDCGHHHHHHHHEHHHGHGHHHGFHGGHGGHHHHHHHHFHHHFHHHGHHHHHHHHHFGGHHHHGGGEGAFHHGHHGGHHHHGHFBGFHGHGHGHGGHGGF@DAFEFBECAB=A.0BFFFEDF=A9.@D.-AAFFFA-:-..:.9/0:;0..-..0000::BFB09:: +@M01368:8:000000000-A3GHV:1:1113:21679:18011/2:R1:GATCACAG:R2:TTAGTAAG:F1:GTCT:F2:TATG +TCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCTGTCTCTTATACACATCTGACGCTGCCGACGACCGAGCTAGTTGAGATC ++ +GGGGGGGGGHHHHHFHHHGHHHGFGHGGHGGHHHHHHGHHHHHHHGGGGFHHHHHHEGHHHHHHHHFGGFCGEHFHFGEGHHHFGGGGGHHHGGGEHGGGGGHHHHHHGDCCCGHHHHDGGGGCD/FCC-><FGFGEHEBDF0DGFFFFGGFFHCE.;.A;CF0;BFBFFGF/9C0;0CFGGF0F09FGGGFFFBBFEFFFB0FFEF0;09.:..9--::---;;-;;F00000:.00 +@M01368:8:000000000-A3GHV:1:1113:25528:14016/2:R1:CCTACAAG:R2:GTATCCGA:F1:CATT:F2:CATC +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +GGGGGGGGGHHHHHHHHHHHHHHHHGHGHHHHHHHHHGGGGHHHHHHHHHFHHHHHHHHHHHHGGGGGHHHHHHHGHHHHHHHHHHGGHHHHGHHGGHHHEGGGGGHHHHHHHHHGHHHHHGHHHHHGGHGHGHHGGGGGGGGGFEACFFFFFFFFFFFFFFFDFFFAFFFFBFAB@EFAAEFFFFF.ACF.BBFFEBFFFEB;FFFFFFFA/BFBFBBBFFFBFFFFFED.>DFFF. +@M01368:8:000000000-A3GHV:1:1113:5741:16959/2:R1:TAGTAAGT:R2:GTATCCGA:F1:ATGT:F2:CATC +GGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +GGGGGGGGGHHHHHHHGHHHHHHHHGHHHHGHDGHHHGGGGHHHHHHHHHHHHHHHGHHHHHGGGEGGHHHHFHHHHHHHHHHHHHHHHHHHHHHGHHGHHGGGGGHHHHHHHHHHHGHHHFHHHHHHGHGHGHGGGGCGGFGGFFFFFFFFFFFFFBFFFFFFFFFFFFFFAFFFFEAEFFFFFFFFFFF9BFFBFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFADAB-/BF. +@M01368:8:000000000-A3GHV:1:1114:10130:11959/2:R1:CGTTCCCC:R2:ATCAGAGA:F1:TTAA:F2:CAGA +ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGGAGAGTTCTGTGGGGCCGGGGATCCTTATAAAAAAAATAGA ++ +GGGGGGGGGGHHHHHGGHHHGGGCGGHGHGGGGHHGGGGHHHHHHGGGEGGHHHFGGGGG?E1FE?/EEHHHHHGHHGHHHHGHFHGHGHHGDGGFG2FF2?GHHHHHGCCCFHGHGHHHHGHHFEHHFGHHGHH<1=DGHHHGHHGHGAGAEEDG.CGCGHC0CGBFHGFBBF0ABDDEFF@?--:BB@.;:BF;0.0:0.0:000:BF.-.------.9/;000::0;0:--.000 +@M01368:8:000000000-A3GHV:1:1114:14540:5315/2:R1:CTTTAGTA:R2:CACTTGGG:F1:AGTA:F2:GGTA +CTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCC ++ +GGGGGGGGGHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHHGHHHHHHHGHHHHFHFHHHGGGGGHHHHHHHHHHHHHGHHHHHHHHGGGGGGHHHFHHHGHHHHHHHHHHHHHHHHHHGHGHHHGGGFGHHHHHHHHFHHHHHF?1FHHGHGHGHGHHGGFFFFDBFBE;BCC.:BFFFFFFFFFFFFFF;AFFFFF-=-.AEDEFFFFF..9A;9FFFF0FFFFE00FFF0:BA. +@M01368:8:000000000-A3GHV:1:1114:15066:16302/2:R1:TAAATAAG:R2:TTATTATT:F1:ACAT:F2:ATGT +CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGTGG ++ +GGGGGGGGGHHHHHHHHHHHHHHHDGHHHHFHHGHHGHHHHHGGGGEHHHHFHHFF5FHHFEGHHHGDHGGHGHGFGGGEHFGHHGGGGGHGHHHGHHFHHB3FGHHFGGGG?GFFHCCEBGFFECCDFEGFCFGCHHGFDDHHHGHHCFGGGGGFBFDGFG?-:..AFG.-C0C009;:00;00:9/:CEFFF?AE::9;9?0:FEF0;0..--./00::B/090000000;A.... +@M01368:8:000000000-A3GHV:1:1114:16639:15258/2:R1:CCTAAATA:R2:TTATTATT:F1:GCCC:F2:ATGT +CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCGCACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATG ++ +GGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHHGGGGGHGIHHHIH5DEGHHHF?FGHGGHGGHEGGHFHHGHGEHHGGGGGFFFGHFBG2GHEBGHHGHGGEG/GFGABEDFGHEED?GGHHFFGGGCFEGD/GFHFFGEFGCGG?CC??D-EF@EEEFGCDDBBFGGGEBBFFF09090A.BFGA.9CCA0;EBAB00BBFF.@-./;BB;BFFF0:00099AAFFF0 +@M01368:8:000000000-A3GHV:1:1114:2404:13066/2:R1:TCCTACAA:R2:ATGGATCA:F1:GCAT:F2:CAGG +CTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGACCATACTTACTAAAGTGTGTTAATTAATTAATGCTTGTAGGACTGTCTCTTATACACATT ++ +GGGGGGGGGHHHHHHHHHFFHHHHGGGGGHFFFHHFHHHHHHHHHHHHHHHGFEGGGHGEDFCDFHGHFG@@DGGHHHHHHGGGGCGGGGGEHGGCGBB?CF99EGFGGFGG?D9CFFFF/BBFFFFFEF9BFFAFFFFEFFFFFFFFFFFFFFFFFFFFF.FFBBFFFFFFFFFFFF-9;;;BFFFFFB9BFBFBFABFFEFFFFFFFFFF::BFFBFFFF.9//;FFFFF/BFFB/
--- a/test-data/paired_collection_example_results3.txt Tue Jul 30 06:26:49 2019 -0400 +++ b/test-data/paired_collection_example_results3.txt Fri Oct 08 09:57:52 2021 +0000 @@ -3,11 +3,13 @@ ========================== Input filename: input_1.fastq Trimming mode: paired-end -Trim Galore version: 0.6.3 -Cutadapt version: 2.4 -Number of cores used for trimming: 1 +Trim Galore version: 0.6.7 +Cutadapt version: 3.4 +Python version: could not detect +Number of cores used for trimming: 4 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 +Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp @@ -16,10 +18,10 @@ Length cut-off for read 2: 35 bp (default) -This is cutadapt 2.4 with Python 3.7.3 -Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq -Processing reads on 1 core in single-end mode ... -Finished in 0.01 s (72 us/read; 0.83 M reads/minute). +This is cutadapt 3.4 with Python 3.9.6 +Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq +Processing reads on 4 cores in single-end mode ... +Finished in 0.01 s (115 µs/read; 0.52 M reads/minute). === Summary === @@ -33,10 +35,10 @@ === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times. +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times No. of allowed errors: -0-9 bp: 0; 10-12 bp: 1 +1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 9.6% @@ -85,11 +87,13 @@ ========================== Input filename: input_2.fastq Trimming mode: paired-end -Trim Galore version: 0.6.3 -Cutadapt version: 2.4 -Number of cores used for trimming: 1 +Trim Galore version: 0.6.7 +Cutadapt version: 3.4 +Python version: could not detect +Number of cores used for trimming: 4 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 +Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp @@ -98,10 +102,10 @@ Length cut-off for read 2: 35 bp (default) -This is cutadapt 2.4 with Python 3.7.3 -Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq -Processing reads on 1 core in single-end mode ... -Finished in 0.01 s (55 us/read; 1.08 M reads/minute). +This is cutadapt 3.4 with Python 3.9.6 +Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq +Processing reads on 4 cores in single-end mode ... +Finished in 0.02 s (159 µs/read; 0.38 M reads/minute). === Summary === @@ -115,10 +119,10 @@ === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times. +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times No. of allowed errors: -0-9 bp: 0; 10-12 bp: 1 +1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 12.1%
--- a/test-data/paired_collection_example_results3gz.txt Tue Jul 30 06:26:49 2019 -0400 +++ b/test-data/paired_collection_example_results3gz.txt Fri Oct 08 09:57:52 2021 +0000 @@ -3,11 +3,13 @@ ========================== Input filename: input_1.fastq.gz Trimming mode: paired-end -Trim Galore version: 0.6.3 -Cutadapt version: 2.4 -Number of cores used for trimming: 1 +Trim Galore version: 0.6.7 +Cutadapt version: 3.4 +Python version: could not detect +Number of cores used for trimming: 4 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 +Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp @@ -17,10 +19,10 @@ Output file will be GZIP compressed -This is cutadapt 2.4 with Python 3.7.3 -Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz -Processing reads on 1 core in single-end mode ... -Finished in 0.02 s (176 us/read; 0.34 M reads/minute). +This is cutadapt 3.4 with Python 3.9.6 +Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz +Processing reads on 4 cores in single-end mode ... +Finished in 0.01 s (129 µs/read; 0.47 M reads/minute). === Summary === @@ -34,10 +36,10 @@ === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times. +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times No. of allowed errors: -0-9 bp: 0; 10-12 bp: 1 +1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 9.6% @@ -86,11 +88,13 @@ ========================== Input filename: input_2.fastq.gz Trimming mode: paired-end -Trim Galore version: 0.6.3 -Cutadapt version: 2.4 -Number of cores used for trimming: 1 +Trim Galore version: 0.6.7 +Cutadapt version: 3.4 +Python version: could not detect +Number of cores used for trimming: 4 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 +Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp @@ -100,10 +104,10 @@ Output file will be GZIP compressed -This is cutadapt 2.4 with Python 3.7.3 -Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz -Processing reads on 1 core in single-end mode ... -Finished in 0.02 s (169 us/read; 0.36 M reads/minute). +This is cutadapt 3.4 with Python 3.9.6 +Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz +Processing reads on 4 cores in single-end mode ... +Finished in 0.04 s (395 µs/read; 0.15 M reads/minute). === Summary === @@ -117,10 +121,10 @@ === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times. +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times No. of allowed errors: -0-9 bp: 0; 10-12 bp: 1 +1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 12.1%
--- a/test-data/paired_example_results2.txt Tue Jul 30 06:26:49 2019 -0400 +++ b/test-data/paired_example_results2.txt Fri Oct 08 09:57:52 2021 +0000 @@ -3,21 +3,23 @@ ========================== Input filename: input_1.fastq Trimming mode: paired-end -Trim Galore version: 0.6.3 -Cutadapt version: 2.4 -Number of cores used for trimming: 1 +Trim Galore version: 0.6.7 +Cutadapt version: 3.4 +Python version: could not detect +Number of cores used for trimming: 4 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 +Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp -This is cutadapt 2.4 with Python 3.7.3 -Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq -Processing reads on 1 core in single-end mode ... -Finished in 0.01 s (75 us/read; 0.80 M reads/minute). +This is cutadapt 3.4 with Python 3.9.6 +Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq +Processing reads on 4 cores in single-end mode ... +Finished in 0.01 s (117 µs/read; 0.51 M reads/minute). === Summary === @@ -31,10 +33,10 @@ === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times. +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times No. of allowed errors: -0-9 bp: 0; 10-12 bp: 1 +1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 9.6% @@ -83,21 +85,23 @@ ========================== Input filename: input_2.fastq Trimming mode: paired-end -Trim Galore version: 0.6.3 -Cutadapt version: 2.4 -Number of cores used for trimming: 1 +Trim Galore version: 0.6.7 +Cutadapt version: 3.4 +Python version: could not detect +Number of cores used for trimming: 4 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 +Using Nextera adapter for trimming (count: 29). Second best hit was smallRNA (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp -This is cutadapt 2.4 with Python 3.7.3 -Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq -Processing reads on 1 core in single-end mode ... -Finished in 0.00 s (50 us/read; 1.19 M reads/minute). +This is cutadapt 3.4 with Python 3.9.6 +Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq +Processing reads on 4 cores in single-end mode ... +Finished in 0.03 s (256 µs/read; 0.23 M reads/minute). === Summary === @@ -111,10 +115,10 @@ === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times. +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times No. of allowed errors: -0-9 bp: 0; 10-12 bp: 1 +1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 12.1%
--- a/test-data/paired_example_results2gz.txt Tue Jul 30 06:26:49 2019 -0400 +++ b/test-data/paired_example_results2gz.txt Fri Oct 08 09:57:52 2021 +0000 @@ -3,11 +3,13 @@ ========================== Input filename: input_1.fastq.gz Trimming mode: paired-end -Trim Galore version: 0.6.3 -Cutadapt version: 2.4 -Number of cores used for trimming: 1 +Trim Galore version: 0.6.7 +Cutadapt version: 3.4 +Python version: could not detect +Number of cores used for trimming: 4 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 +Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp @@ -15,10 +17,10 @@ Output file will be GZIP compressed -This is cutadapt 2.4 with Python 3.7.3 -Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz -Processing reads on 1 core in single-end mode ... -Finished in 0.03 s (287 us/read; 0.21 M reads/minute). +This is cutadapt 3.4 with Python 3.9.6 +Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_1.fastq.gz +Processing reads on 4 cores in single-end mode ... +Finished in 0.01 s (114 µs/read; 0.53 M reads/minute). === Summary === @@ -32,10 +34,10 @@ === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times. +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 52 times No. of allowed errors: -0-9 bp: 0; 10-12 bp: 1 +1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 9.6% @@ -84,11 +86,13 @@ ========================== Input filename: input_2.fastq.gz Trimming mode: paired-end -Trim Galore version: 0.6.3 -Cutadapt version: 2.4 -Number of cores used for trimming: 1 +Trim Galore version: 0.6.7 +Cutadapt version: 3.4 +Python version: could not detect +Number of cores used for trimming: 4 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 +Using Nextera adapter for trimming (count: 29). Second best hit was Illumina (count: 0) Adapter sequence: 'CTGTCTCTTATA' (Nextera Transposase sequence; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp @@ -96,10 +100,10 @@ Output file will be GZIP compressed -This is cutadapt 2.4 with Python 3.7.3 -Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz -Processing reads on 1 core in single-end mode ... -Finished in 0.02 s (170 us/read; 0.35 M reads/minute). +This is cutadapt 3.4 with Python 3.9.6 +Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a CTGTCTCTTATA input_2.fastq.gz +Processing reads on 4 cores in single-end mode ... +Finished in 0.02 s (232 µs/read; 0.26 M reads/minute). === Summary === @@ -113,10 +117,10 @@ === Adapter 1 === -Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times. +Sequence: CTGTCTCTTATA; Type: regular 3'; Length: 12; Trimmed: 58 times No. of allowed errors: -0-9 bp: 0; 10-12 bp: 1 +1-9 bp: 0; 10-12 bp: 1 Bases preceding removed adapters: A: 12.1%
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/paired_hardtrimmed3_pair1_.fastqsanger Fri Oct 08 09:57:52 2021 +0000 @@ -0,0 +1,396 @@ +@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 +TCGCACCTACGTTCAATATT ++ +FFEFE;DFFFDFBFF/9BFB +@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 +GACCTGTGATCCATCGTGAT ++ +FF//;BFFFFFBF/9D:A// +@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 +TATTATTTATCGCACCTACG ++ +FFEFBFFFFFFFFFF:FFF. +@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 +TTTAAGGGGAACGGGTGGGG ++ +FBFFFFFFBF..A..;@B-- +@M01368:8:000000000-A3GHV:1:1101:5446:12248/1 +AAGGGGAACGTGTGGGCTAT ++ +FFFBFFFFFFEFFFFFEFFF +@M01368:8:000000000-A3GHV:1:1101:5861:6452/1 +TGATCCATCGTGATGTCTTT ++ +FFFFFBFFBD.AFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1102:10403:6021/1 +ACCCTATGTCGCAGTATCTG ++ +..9..9/9;BEF;A:9/BFF +@M01368:8:000000000-A3GHV:1:1102:10677:23253/1 +AATTCTGTCTCTTATACACA ++ +F0FFFFFFFFFFFFFFFFF: +@M01368:8:000000000-A3GHV:1:1102:13809:1733/1 +GTCTCTTATACACATCTCCG ++ +FBBBFFFFFBFF0BFBF9F. +@M01368:8:000000000-A3GHV:1:1102:17584:10050/1 +AATCTCGTATGCCGTCTTCT ++ +FF;.BF9ADF/9;A;DFFF0 +@M01368:8:000000000-A3GHV:1:1102:18842:24844/1 +TCTGTCTTTGATTCCTGCCT ++ +BBBFBFBFFF//BFB/BFF/ +@M01368:8:000000000-A3GHV:1:1102:21788:11027/1 +ACTAAAGTGTGTTAATTCTG ++ +FBFF0FFBBBBFBFBFFFF0 +@M01368:8:000000000-A3GHV:1:1102:5728:8777/1 +CCTGAAGTAGGAACCAGATG ++ +FFFFBBFFFFF/FFEFFFBB +@M01368:8:000000000-A3GHV:1:1102:5983:21093/1 +TATTATTTATCGCACCTACG ++ +F0BFFFBBFF0EFFFFFFFA +@M01368:8:000000000-A3GHV:1:1102:7765:18353/1 +CCTATGTCGCAGTATCTGTC ++ +.::FBFBFFE-A9:BFFFF0 +@M01368:8:000000000-A3GHV:1:1103:10405:17879/1 +TATTATTTATCGCACCTACG ++ +FBFFFFFFFFFFFFFFFFF? +@M01368:8:000000000-A3GHV:1:1103:13493:8205/1 +GATTCCTGCCTCATCCTATT ++ +FFFFFFFFBFBBBFFFFEFF +@M01368:8:000000000-A3GHV:1:1103:14060:21273/1 +TATTACTGTCTCTTATACAC ++ +:FFFBB/BFFFFFBFFFFB/ +@M01368:8:000000000-A3GHV:1:1103:14528:12993/1 +TCGCAGTATCTGTCTTTGAT ++ +BFEA-A.B//9F9F//;BBB +@M01368:8:000000000-A3GHV:1:1103:17657:20856/1 +TGCCTCATCCTATTATTTAT ++ +FFFFFFFFFFF/FFFFBFF9 +@M01368:8:000000000-A3GHV:1:1103:23099:3794/1 +GCGAATCTCGGATGCCGTCT ++ +FFFF@;.9BF..;-./-A=F +@M01368:8:000000000-A3GHV:1:1103:25465:20010/1 +AAAAAAAAAAAACAACAGAC ++ +.B/;:>---;--..;.../9 +@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 +GAGACTAAGGCGAATTTCGT ++ +-B?DFFF/B/B?---.//;. +@M01368:8:000000000-A3GHV:1:1103:6074:6549/1 +GTATCTGTCTTTGATTCCTG ++ +A./FFFFFFFFFFFF/BFF/ +@M01368:8:000000000-A3GHV:1:1104:11572:8264/1 +TATTATTTATCGCACCTACG ++ +B/BFFFF/BFFE?EFAFEF9 +@M01368:8:000000000-A3GHV:1:1104:11928:24197/1 +TAGGAACCAGATGTCGGATA ++ +FFFFFFFFFFFFFFFFF@D9 +@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 +TCTGTCTTTGATTCCTGCCT ++ +;FBBFBFB///;99999BA9 +@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 +ACCCAGTAGACTAGTGCGTC ++ +9...:.9//9////;/9-.- +@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 +GGGTGATAGACCTGTGATCC ++ +BF./AAF/BFFBBF/F/BFB +@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 +TTCCTGCCTCATCCTATTAT ++ +//;/;9/A/;/9BFFFBFBB +@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 +CGTGATGTCTTATTTAAGGG ++ +F?.AFFF/BFFEFFFFFEFF +@M01368:8:000000000-A3GHV:1:1104:7756:4776/1 +GTCGCAGTATCTGTCTTTGA ++ +FFBEFFF:9FFFFBBFFFF/ +@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 +TGTCGGATACAGTTCACTTT ++ +FFBFFFFFFFFFFFFEFFF0 +@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 +TGAAGTAGGAACCAGATGTC ++ +FF/FFFFFFFFFFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 +TGGGCTATTTAGGTTTTATG ++ +FF?B---;B99//9/;A//9 +@M01368:8:000000000-A3GHV:1:1105:15502:11453/1 +CTGTCTCTTATACACATCTC ++ +FFFFEFFFFFFFFBFFFBFF +@M01368:8:000000000-A3GHV:1:1105:16787:9771/1 +GACCTGTGATCCATCGTGAT ++ +FFFFFFBFFFFFFFFFEFF/ +@M01368:8:000000000-A3GHV:1:1105:17564:15723/1 +TTAGGTTTTATGACCCTGAA ++ +FFBBFBBFFFFFE1:BFFB0 +@M01368:8:000000000-A3GHV:1:1105:18108:12091/1 +GATACCTGTCTCTTATACAC ++ +F-A.B//;BFFEF//BB/// +@M01368:8:000000000-A3GHV:1:1105:18331:16683/1 +CGCACCTACGTTCAATATTA ++ +B.DABFFFFFFB.//:FB// +@M01368:8:000000000-A3GHV:1:1105:20577:18421/1 +CTTTGATTCCTGCCTCATCC ++ +FBB/:FF9BF/BF.BBFF/: +@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 +ATTCCTGCCTCATCCTATTT ++ +BFBFFBFF.0:000BFFBF0 +@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 +ACCTGTGATCCATCGTGATG ++ +FFFFFFF/9FFFFBDEFFFF +@M01368:8:000000000-A3GHV:1:1105:7169:17145/1 +GAACATACTTACTAAAGTGT ++ +-9@DDBFFFFFFFFBBFFF0 +@M01368:8:000000000-A3GHV:1:1106:15481:16176/1 +TGTGGGCTATTTAGGTTTTT ++ +FE:AAFEF.BFFFFF;9FF. +@M01368:8:000000000-A3GHV:1:1106:17389:18671/1 +GCAGTATCTGGCTTTGATTC ++ +..;A;/B/;B//;;//BBF/ +@M01368:8:000000000-A3GHV:1:1106:22927:3863/1 +CTCCGAGCCCACGAGACTAA ++ +//FFF-=--.99=--.9/// +@M01368:8:000000000-A3GHV:1:1106:23625:4236/1 +ATTCCTGCCTCATCCTATTA ++ +EFFFFFFFAF///9FF/BB/ +@M01368:8:000000000-A3GHV:1:1106:26031:14826/1 +GTATGCCGTCTTCTGCTTGA ++ +AFFFFFF;;BFE/BFFFFB: +@M01368:8:000000000-A3GHV:1:1106:27607:15990/1 +AACTACAACCCACGGACGCA ++ +;B.//////;...---9--- +@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 +TATTATTTATCGCACCTACG ++ +F/FFBFFFFFFADE>BFFF9 +@M01368:8:000000000-A3GHV:1:1107:14451:26748/1 +TTTAGGTTTTATGACCCTGA ++ +FBFFFF;FFEBFBFFB./9. +@M01368:8:000000000-A3GHV:1:1107:18714:24788/1 +TTTAGGTTTTATGACCCTGA ++ +FFFFFFBFFFFFBBFFBBF/ +@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 +CCTGAAGTAGGAACCAGATG ++ +FFFFFFFFFFFFBFF.AA/9 +@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 +ACTAAGGCGAATCTCGTATG ++ +9DFFF/FFBF;-.:;.9A// +@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 +TCAATATTACAGGCGAACAT ++ +FFFFFBF//:BFBACF--9/ +@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 +ATCCTATTATTTATCGCACC ++ +FFFFFFFFFFF/BFBEFFC- +@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 +CTGTCTCTTATACACCTCTC ++ +BFFFBBFFF//B/////-;9 +@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 +GACCTGTGATCCATCGTGAT ++ +FFFFBF;BBFFBFFFFE?F/ +@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 +GAACCCTGTCTCTTATACAC ++ +EFF?EFFFF0BFFFFBBB00 +@M01368:8:000000000-A3GHV:1:1108:14328:14751/1 +GGATACCTGTCTCTTATACA ++ +EFFFBF/BF/FFEFFBFFF/ +@M01368:8:000000000-A3GHV:1:1108:16350:22621/1 +TGGGCTATTTAGGTTTTATG ++ +BE.99FAFFFFFFF/;EFF/ +@M01368:8:000000000-A3GHV:1:1108:21791:23272/1 +TGTGGGCTATTTAGGTTTTA ++ +EEFEFFFBFBFBBFFFFF./ +@M01368:8:000000000-A3GHV:1:1108:23314:26089/1 +TGAAGTAGGAACCAGATGTG ++ +BB./:BFFF/F;AA?F//// +@M01368:8:000000000-A3GHV:1:1108:25084:18127/1 +TGTGATCCATCGTGATGTCT ++ +FF9FBFFB/F/.;FFFF/BB +@M01368:8:000000000-A3GHV:1:1108:7626:10048/1 +TATGCCGTCTTCTGCTTGAA ++ +AAFFFFFFAFABBFFFB/;; +@M01368:8:000000000-A3GHV:1:1109:11270:12255/1 +AGACTAAGGCGAATCTCGTT ++ +-@DBFFFFBFD---.;BAA. +@M01368:8:000000000-A3GHV:1:1109:19224:11655/1 +CTTATACACATCTCCGAGCC ++ +FFB/;F/99/FBFFBDCF;B +@M01368:8:000000000-A3GHV:1:1109:21978:9490/1 +GGCGAATCTCGTATGCAGTT ++ +FFFFA;;;FF..9//;/;;/ +@M01368:8:000000000-A3GHV:1:1109:22910:4079/1 +GATACAGTCCACTTTAGCTA ++ +CCDFFFFFFF0BFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1109:24217:19077/1 +TGCCTCATCCTATTACTGTC ++ +9;F./B//BBFB9////99/ +@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 +AGGCGAATATCGTATGCCGT ++ +BF/99D-;.//.../9;B-. +@M01368:8:000000000-A3GHV:1:1109:27103:7153/1 +GACCCTGAAGTAGCTGTCTC ++ +BBFFBFFFF///;/9B;/// +@M01368:8:000000000-A3GHV:1:1109:8763:7859/1 +GGGCTATTTAGGCTTTATGC ++ +EDAA.A/BFFFFFFFFFEB/ +@M01368:8:000000000-A3GHV:1:1110:12808:18367/1 +AAGGGGAACGTGTGGGCCTG ++ +BFBFFB9..AF?FFFFFFF9 +@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 +GTATCTGTCTTTGATTCCTG ++ +9-/;B9BF/9BB//B/;/// +@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 +ATGACCCTGAAGTAGGAACC ++ +FFFFFBFFF.BFFFBFBBA; +@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 +AGGGTGATAGACCTGTGATC ++ +/99;;:.//:FFFFBBF//9 +@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 +CTTATACACATCTCCGAGCC ++ +BFFFFB/BFFBFFF/ADCAD +@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 +TGCTTGCAAAAAAAAAATAG ++ +/BBFFF.9/FF?DA=@9./9 +@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 +CTGTCTTTGATTCCTGCCTC ++ +FBBFFFF/BBFFFFBFF9B; +@M01368:8:000000000-A3GHV:1:1111:13195:14562/1 +ACATCTCCGAGCCCACGAGA ++ +FFEFFFFED-DCFF?.:BFA +@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 +ATTAAAGTGTGTTAATTAAT ++ +B//B/;/;9//9//;B/:B9 +@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 +CATCGTGATGTCTTATTTAG ++ +B/FBDE9/;99/B/BFFEF/ +@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 +CACATCTCCGAGCCCACGAG ++ +FFFFB:FFFFD=CAEEEF>- +@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 +TACGTTCAATATTACAGGCG ++ +FFFBA/F//BFFFBF/;/A- +@M01368:8:000000000-A3GHV:1:1111:9655:13916/1 +GTCGCAGTATCTGTCTTTGA ++ +F/B?A@F-9BBFF//BB/9/ +@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 +CGAATCTCGTATGCCGTCTT ++ +FAD--.99;A?////:.9A. +@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 +ATGACCTTGAAGTAGGAACC ++ +FFF/BF//9/B//9:B//.. +@M01368:8:000000000-A3GHV:1:1112:9832:6701/1 +TTTATGACCCTGAAGTAGGA ++ +FFFFFFF/B./FB///;BF/ +@M01368:8:000000000-A3GHV:1:1113:13532:8618/1 +AGCTACCTCCAAGTGTTATG ++ +FF/BFFB/:.BB//;FBFFF +@M01368:8:000000000-A3GHV:1:1113:21679:18011/1 +AGACTAAGGCGAAGTTCATT ++ +;-;AFFE;/99-9--/;/// +@M01368:8:000000000-A3GHV:1:1113:25528:14016/1 +TCGTGATGTCTTATTTAAGG ++ +FFFEABF//99FFEFFFBFF +@M01368:8:000000000-A3GHV:1:1113:5741:16959/1 +GGGCTATTTAGGTTTTATGA ++ +9AD;.FFFB/B/;FBA/B// +@M01368:8:000000000-A3GHV:1:1114:10130:11959/1 +GCAACAAACACACATCCAGA ++ +F./B/..9.9:...////// +@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 +TGGGGGCTATTTAGGTTTTT ++ +;A.-=A--:BBFB90;;FE. +@M01368:8:000000000-A3GHV:1:1114:15066:16302/1 +TAATTATTGCTTGTAGGACA ++ +F/;BFF/;/////9/////. +@M01368:8:000000000-A3GHV:1:1114:16639:15258/1 +ACATACTTACTAAATTGTGT ++ +-9-99/B9F/://///99/; +@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 +ATCTGTCTATTATACACATC ++ +FFFFFFFFFFFFFFFFEFF0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/paired_hardtrimmed3_pair2_.fastqsanger Fri Oct 08 09:57:52 2021 +0000 @@ -0,0 +1,396 @@ +@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 +TCGCACCTACGTTCAATATT ++ +FFEFE;DFFFDFBFF/9BFB +@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 +GACCTGTGATCCATCGTGAT ++ +FF//;BFFFFFBF/9D:A// +@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 +TATTATTTATCGCACCTACG ++ +FFEFBFFFFFFFFFF:FFF. +@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 +TTTAAGGGGAACGGGTGGGG ++ +FBFFFFFFBF..A..;@B-- +@M01368:8:000000000-A3GHV:1:1101:5446:12248/1 +AAGGGGAACGTGTGGGCTAT ++ +FFFBFFFFFFEFFFFFEFFF +@M01368:8:000000000-A3GHV:1:1101:5861:6452/1 +TGATCCATCGTGATGTCTTT ++ +FFFFFBFFBD.AFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1102:10403:6021/1 +ACCCTATGTCGCAGTATCTG ++ +..9..9/9;BEF;A:9/BFF +@M01368:8:000000000-A3GHV:1:1102:10677:23253/1 +AATTCTGTCTCTTATACACA ++ +F0FFFFFFFFFFFFFFFFF: +@M01368:8:000000000-A3GHV:1:1102:13809:1733/1 +GTCTCTTATACACATCTCCG ++ +FBBBFFFFFBFF0BFBF9F. +@M01368:8:000000000-A3GHV:1:1102:17584:10050/1 +AATCTCGTATGCCGTCTTCT ++ +FF;.BF9ADF/9;A;DFFF0 +@M01368:8:000000000-A3GHV:1:1102:18842:24844/1 +TCTGTCTTTGATTCCTGCCT ++ +BBBFBFBFFF//BFB/BFF/ +@M01368:8:000000000-A3GHV:1:1102:21788:11027/1 +ACTAAAGTGTGTTAATTCTG ++ +FBFF0FFBBBBFBFBFFFF0 +@M01368:8:000000000-A3GHV:1:1102:5728:8777/1 +CCTGAAGTAGGAACCAGATG ++ +FFFFBBFFFFF/FFEFFFBB +@M01368:8:000000000-A3GHV:1:1102:5983:21093/1 +TATTATTTATCGCACCTACG ++ +F0BFFFBBFF0EFFFFFFFA +@M01368:8:000000000-A3GHV:1:1102:7765:18353/1 +CCTATGTCGCAGTATCTGTC ++ +.::FBFBFFE-A9:BFFFF0 +@M01368:8:000000000-A3GHV:1:1103:10405:17879/1 +TATTATTTATCGCACCTACG ++ +FBFFFFFFFFFFFFFFFFF? +@M01368:8:000000000-A3GHV:1:1103:13493:8205/1 +GATTCCTGCCTCATCCTATT ++ +FFFFFFFFBFBBBFFFFEFF +@M01368:8:000000000-A3GHV:1:1103:14060:21273/1 +TATTACTGTCTCTTATACAC ++ +:FFFBB/BFFFFFBFFFFB/ +@M01368:8:000000000-A3GHV:1:1103:14528:12993/1 +TCGCAGTATCTGTCTTTGAT ++ +BFEA-A.B//9F9F//;BBB +@M01368:8:000000000-A3GHV:1:1103:17657:20856/1 +TGCCTCATCCTATTATTTAT ++ +FFFFFFFFFFF/FFFFBFF9 +@M01368:8:000000000-A3GHV:1:1103:23099:3794/1 +GCGAATCTCGGATGCCGTCT ++ +FFFF@;.9BF..;-./-A=F +@M01368:8:000000000-A3GHV:1:1103:25465:20010/1 +AAAAAAAAAAAACAACAGAC ++ +.B/;:>---;--..;.../9 +@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 +GAGACTAAGGCGAATTTCGT ++ +-B?DFFF/B/B?---.//;. +@M01368:8:000000000-A3GHV:1:1103:6074:6549/1 +GTATCTGTCTTTGATTCCTG ++ +A./FFFFFFFFFFFF/BFF/ +@M01368:8:000000000-A3GHV:1:1104:11572:8264/1 +TATTATTTATCGCACCTACG ++ +B/BFFFF/BFFE?EFAFEF9 +@M01368:8:000000000-A3GHV:1:1104:11928:24197/1 +TAGGAACCAGATGTCGGATA ++ +FFFFFFFFFFFFFFFFF@D9 +@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 +TCTGTCTTTGATTCCTGCCT ++ +;FBBFBFB///;99999BA9 +@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 +ACCCAGTAGACTAGTGCGTC ++ +9...:.9//9////;/9-.- +@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 +GGGTGATAGACCTGTGATCC ++ +BF./AAF/BFFBBF/F/BFB +@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 +TTCCTGCCTCATCCTATTAT ++ +//;/;9/A/;/9BFFFBFBB +@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 +CGTGATGTCTTATTTAAGGG ++ +F?.AFFF/BFFEFFFFFEFF +@M01368:8:000000000-A3GHV:1:1104:7756:4776/1 +GTCGCAGTATCTGTCTTTGA ++ +FFBEFFF:9FFFFBBFFFF/ +@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 +TGTCGGATACAGTTCACTTT ++ +FFBFFFFFFFFFFFFEFFF0 +@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 +TGAAGTAGGAACCAGATGTC ++ +FF/FFFFFFFFFFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 +TGGGCTATTTAGGTTTTATG ++ +FF?B---;B99//9/;A//9 +@M01368:8:000000000-A3GHV:1:1105:15502:11453/1 +CTGTCTCTTATACACATCTC ++ +FFFFEFFFFFFFFBFFFBFF +@M01368:8:000000000-A3GHV:1:1105:16787:9771/1 +GACCTGTGATCCATCGTGAT ++ +FFFFFFBFFFFFFFFFEFF/ +@M01368:8:000000000-A3GHV:1:1105:17564:15723/1 +TTAGGTTTTATGACCCTGAA ++ +FFBBFBBFFFFFE1:BFFB0 +@M01368:8:000000000-A3GHV:1:1105:18108:12091/1 +GATACCTGTCTCTTATACAC ++ +F-A.B//;BFFEF//BB/// +@M01368:8:000000000-A3GHV:1:1105:18331:16683/1 +CGCACCTACGTTCAATATTA ++ +B.DABFFFFFFB.//:FB// +@M01368:8:000000000-A3GHV:1:1105:20577:18421/1 +CTTTGATTCCTGCCTCATCC ++ +FBB/:FF9BF/BF.BBFF/: +@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 +ATTCCTGCCTCATCCTATTT ++ +BFBFFBFF.0:000BFFBF0 +@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 +ACCTGTGATCCATCGTGATG ++ +FFFFFFF/9FFFFBDEFFFF +@M01368:8:000000000-A3GHV:1:1105:7169:17145/1 +GAACATACTTACTAAAGTGT ++ +-9@DDBFFFFFFFFBBFFF0 +@M01368:8:000000000-A3GHV:1:1106:15481:16176/1 +TGTGGGCTATTTAGGTTTTT ++ +FE:AAFEF.BFFFFF;9FF. +@M01368:8:000000000-A3GHV:1:1106:17389:18671/1 +GCAGTATCTGGCTTTGATTC ++ +..;A;/B/;B//;;//BBF/ +@M01368:8:000000000-A3GHV:1:1106:22927:3863/1 +CTCCGAGCCCACGAGACTAA ++ +//FFF-=--.99=--.9/// +@M01368:8:000000000-A3GHV:1:1106:23625:4236/1 +ATTCCTGCCTCATCCTATTA ++ +EFFFFFFFAF///9FF/BB/ +@M01368:8:000000000-A3GHV:1:1106:26031:14826/1 +GTATGCCGTCTTCTGCTTGA ++ +AFFFFFF;;BFE/BFFFFB: +@M01368:8:000000000-A3GHV:1:1106:27607:15990/1 +AACTACAACCCACGGACGCA ++ +;B.//////;...---9--- +@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 +TATTATTTATCGCACCTACG ++ +F/FFBFFFFFFADE>BFFF9 +@M01368:8:000000000-A3GHV:1:1107:14451:26748/1 +TTTAGGTTTTATGACCCTGA ++ +FBFFFF;FFEBFBFFB./9. +@M01368:8:000000000-A3GHV:1:1107:18714:24788/1 +TTTAGGTTTTATGACCCTGA ++ +FFFFFFBFFFFFBBFFBBF/ +@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 +CCTGAAGTAGGAACCAGATG ++ +FFFFFFFFFFFFBFF.AA/9 +@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 +ACTAAGGCGAATCTCGTATG ++ +9DFFF/FFBF;-.:;.9A// +@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 +TCAATATTACAGGCGAACAT ++ +FFFFFBF//:BFBACF--9/ +@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 +ATCCTATTATTTATCGCACC ++ +FFFFFFFFFFF/BFBEFFC- +@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 +CTGTCTCTTATACACCTCTC ++ +BFFFBBFFF//B/////-;9 +@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 +GACCTGTGATCCATCGTGAT ++ +FFFFBF;BBFFBFFFFE?F/ +@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 +GAACCCTGTCTCTTATACAC ++ +EFF?EFFFF0BFFFFBBB00 +@M01368:8:000000000-A3GHV:1:1108:14328:14751/1 +GGATACCTGTCTCTTATACA ++ +EFFFBF/BF/FFEFFBFFF/ +@M01368:8:000000000-A3GHV:1:1108:16350:22621/1 +TGGGCTATTTAGGTTTTATG ++ +BE.99FAFFFFFFF/;EFF/ +@M01368:8:000000000-A3GHV:1:1108:21791:23272/1 +TGTGGGCTATTTAGGTTTTA ++ +EEFEFFFBFBFBBFFFFF./ +@M01368:8:000000000-A3GHV:1:1108:23314:26089/1 +TGAAGTAGGAACCAGATGTG ++ +BB./:BFFF/F;AA?F//// +@M01368:8:000000000-A3GHV:1:1108:25084:18127/1 +TGTGATCCATCGTGATGTCT ++ +FF9FBFFB/F/.;FFFF/BB +@M01368:8:000000000-A3GHV:1:1108:7626:10048/1 +TATGCCGTCTTCTGCTTGAA ++ +AAFFFFFFAFABBFFFB/;; +@M01368:8:000000000-A3GHV:1:1109:11270:12255/1 +AGACTAAGGCGAATCTCGTT ++ +-@DBFFFFBFD---.;BAA. +@M01368:8:000000000-A3GHV:1:1109:19224:11655/1 +CTTATACACATCTCCGAGCC ++ +FFB/;F/99/FBFFBDCF;B +@M01368:8:000000000-A3GHV:1:1109:21978:9490/1 +GGCGAATCTCGTATGCAGTT ++ +FFFFA;;;FF..9//;/;;/ +@M01368:8:000000000-A3GHV:1:1109:22910:4079/1 +GATACAGTCCACTTTAGCTA ++ +CCDFFFFFFF0BFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1109:24217:19077/1 +TGCCTCATCCTATTACTGTC ++ +9;F./B//BBFB9////99/ +@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 +AGGCGAATATCGTATGCCGT ++ +BF/99D-;.//.../9;B-. +@M01368:8:000000000-A3GHV:1:1109:27103:7153/1 +GACCCTGAAGTAGCTGTCTC ++ +BBFFBFFFF///;/9B;/// +@M01368:8:000000000-A3GHV:1:1109:8763:7859/1 +GGGCTATTTAGGCTTTATGC ++ +EDAA.A/BFFFFFFFFFEB/ +@M01368:8:000000000-A3GHV:1:1110:12808:18367/1 +AAGGGGAACGTGTGGGCCTG ++ +BFBFFB9..AF?FFFFFFF9 +@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 +GTATCTGTCTTTGATTCCTG ++ +9-/;B9BF/9BB//B/;/// +@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 +ATGACCCTGAAGTAGGAACC ++ +FFFFFBFFF.BFFFBFBBA; +@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 +AGGGTGATAGACCTGTGATC ++ +/99;;:.//:FFFFBBF//9 +@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 +CTTATACACATCTCCGAGCC ++ +BFFFFB/BFFBFFF/ADCAD +@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 +TGCTTGCAAAAAAAAAATAG ++ +/BBFFF.9/FF?DA=@9./9 +@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 +CTGTCTTTGATTCCTGCCTC ++ +FBBFFFF/BBFFFFBFF9B; +@M01368:8:000000000-A3GHV:1:1111:13195:14562/1 +ACATCTCCGAGCCCACGAGA ++ +FFEFFFFED-DCFF?.:BFA +@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 +ATTAAAGTGTGTTAATTAAT ++ +B//B/;/;9//9//;B/:B9 +@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 +CATCGTGATGTCTTATTTAG ++ +B/FBDE9/;99/B/BFFEF/ +@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 +CACATCTCCGAGCCCACGAG ++ +FFFFB:FFFFD=CAEEEF>- +@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 +TACGTTCAATATTACAGGCG ++ +FFFBA/F//BFFFBF/;/A- +@M01368:8:000000000-A3GHV:1:1111:9655:13916/1 +GTCGCAGTATCTGTCTTTGA ++ +F/B?A@F-9BBFF//BB/9/ +@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 +CGAATCTCGTATGCCGTCTT ++ +FAD--.99;A?////:.9A. +@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 +ATGACCTTGAAGTAGGAACC ++ +FFF/BF//9/B//9:B//.. +@M01368:8:000000000-A3GHV:1:1112:9832:6701/1 +TTTATGACCCTGAAGTAGGA ++ +FFFFFFF/B./FB///;BF/ +@M01368:8:000000000-A3GHV:1:1113:13532:8618/1 +AGCTACCTCCAAGTGTTATG ++ +FF/BFFB/:.BB//;FBFFF +@M01368:8:000000000-A3GHV:1:1113:21679:18011/1 +AGACTAAGGCGAAGTTCATT ++ +;-;AFFE;/99-9--/;/// +@M01368:8:000000000-A3GHV:1:1113:25528:14016/1 +TCGTGATGTCTTATTTAAGG ++ +FFFEABF//99FFEFFFBFF +@M01368:8:000000000-A3GHV:1:1113:5741:16959/1 +GGGCTATTTAGGTTTTATGA ++ +9AD;.FFFB/B/;FBA/B// +@M01368:8:000000000-A3GHV:1:1114:10130:11959/1 +GCAACAAACACACATCCAGA ++ +F./B/..9.9:...////// +@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 +TGGGGGCTATTTAGGTTTTT ++ +;A.-=A--:BBFB90;;FE. +@M01368:8:000000000-A3GHV:1:1114:15066:16302/1 +TAATTATTGCTTGTAGGACA ++ +F/;BFF/;/////9/////. +@M01368:8:000000000-A3GHV:1:1114:16639:15258/1 +ACATACTTACTAAATTGTGT ++ +-9-99/B9F/://///99/; +@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 +ATCTGTCTATTATACACATC ++ +FFFFFFFFFFFFFFFFEFF0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/paired_hardtrimmed5_pair1_.fastqsanger Fri Oct 08 09:57:52 2021 +0000 @@ -0,0 +1,396 @@ +@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 +ATCTGGTTCCTACTTCAGGG ++ +BCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 +GTTATTATTATGTCCTACAA ++ +AAAAAFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 +GTATCCGACATCTGGTTCCT ++ +CCCCCFDDDDDFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 +ATTAATTAACACACTTTAGT ++ +33AA?DFD5BDFGGGFEBDG +@M01368:8:000000000-A3GHV:1:1101:5446:12248/1 +AATTAACACACTTTAGTAAG ++ +CCCCDFFFFCCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1101:5861:6452/1 +ATTATGTCCTACAAGCATTA ++ +ABCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:10403:6021/1 +CGCTCCGGGCCCATAACACT ++ +>A@AAAAD2ADDFFGGGGGF +@M01368:8:000000000-A3GHV:1:1102:10677:23253/1 +CCTTAAATAAGACATCACGA ++ +ABBBBFFFFFFFGGGGGGGC +@M01368:8:000000000-A3GHV:1:1102:13809:1733/1 +ATATTGAACGTAGGTGCGAT ++ +BCCBCFFFFFBFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:17584:10050/1 +ATCAAAGACAGATACTGCGA ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:18842:24844/1 +CACTTGGGGGTAGCTAAAGT ++ +BBBCBFF@CCBBGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:21788:11027/1 +GCCCACACGTTCCCCTTAAA ++ +ABCCBCCBBFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:5728:8777/1 +ATATTGAACGTAGGTGCGAT ++ +BBBBBFFFFFAFGG5GGGGG +@M01368:8:000000000-A3GHV:1:1102:5983:21093/1 +GTATCCGACATCTGGTTCCT ++ +AAABCFCABBCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:7765:18353/1 +CTCCGGGCCCATAACACTTG ++ +CCDCCCCCCBCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1103:10405:17879/1 +GTATCCGACATCTGGTTCCT ++ +CBCCCFCCCCCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1103:13493:8205/1 +GTAGCTAAAGTGAACTGTAT ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1103:14060:21273/1 +GTATCCGACATCTGGTTCCT ++ +AAABAFCCCCCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1103:14528:12993/1 +GCCCATAACACTTGGGGGTA ++ +BBBBBFFFBDFFGFBCG2EG +@M01368:8:000000000-A3GHV:1:1103:17657:20856/1 +AAAGCGAACTGTATCCGACA ++ +ABB?AABBBBFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1103:23099:3794/1 +AAGACAGATACTGCGACATA ++ +BBBBBFFFFFFFGGEEGGFG +@M01368:8:000000000-A3GHV:1:1103:25465:20010/1 +GACATAGGGTGCTCCGGCTC ++ +ABBBBFFFFBAAGGGFEFGG +@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 +AAATAAGACATCACGATGGA ++ +3A3AAFFFFBFFGGG2CF?4 +@M01368:8:000000000-A3GHV:1:1103:6074:6549/1 +TAACACTTGGGGGTAGCTAA ++ +CCCBCFFFFFCCGGGGGGGG +@M01368:8:000000000-A3GHV:1:1104:11572:8264/1 +GTATCCGACATCTGGTTCCT ++ +BABCBFBACCCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1104:11928:24197/1 +ACGTAGGTGCGATAAATAAT ++ +CDDCCFCFFFBCGGGGGGGG +@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 +CACTTGGGGGTAGCTAAAGT ++ +ABA?AFBAB2>AFGGGGFFG +@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 +CACGATGGATCACAGGTCTA ++ +AAABABCAFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 +ATTCAATTGTTATTATTATG ++ +AAAABFFFFFFFGGGGGGGC +@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 +AGCTAAAGTGAACTGTATCC ++ +AAA?AFFF3DFFFGGBGFF3 +@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 +CTACAAGCATTAATTAATTA ++ +BBBBBFFBFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1104:7756:4776/1 +GGCCCATAACACTTGGGGGT ++ +CCCCCCFFFFFFGGGGGGGE +@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 +ATAAATAATAGGATGAGGCA ++ +CCCCBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 +ATTGAACGTAGGTGCGATAA ++ +CDDDDFFFBFFCGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 +TTAGTAAGTATGTTCGCCTG ++ +AA1A1DFFFFFBGG3A111F +@M01368:8:000000000-A3GHV:1:1105:15502:11453/1 +GTCCTACAAGCATTAATTAA ++ +A3AAAFFFFFCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:16787:9771/1 +GTTATTATTATGTCCTACAA ++ +AABCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:17564:15723/1 +TATGCTCGCCTGTAATATTG ++ +EDCCDFFCEEDCGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:18108:12091/1 +TAATAGGATGAGGCAGGAAT ++ +CCCCCFBFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:18331:16683/1 +TCTGGTTCCTACTTCAGGGT ++ +BABA?DFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:20577:18421/1 +GGGGGTAGCTAAAGTGAACT ++ +ABB@3>AAFFFFFEAGGGFG +@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 +TAGCTAAAGTGAACTGTATC ++ +CBCBBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 +TTATGATTATGTCCTACAAG ++ +BAAAAFFFBFFFGGGGGGFF +@M01368:8:000000000-A3GHV:1:1105:7169:17145/1 +AACCTAAATAGCCCACACGT ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1106:15481:16176/1 +CTTTAGTAAGTATGTTCGCC ++ +ABBBBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1106:17389:18671/1 +CCATAACACTTGGGGGTAGC ++ +3>>A@FFFFFFFGGGFEECF +@M01368:8:000000000-A3GHV:1:1106:22927:3863/1 +GTATCCGACATCTGGTTCCT ++ +A>ABAFA?D@AFGGGGFGGG +@M01368:8:000000000-A3GHV:1:1106:23625:4236/1 +TAGCTAAAGTGAACTGTATC ++ +@?AAAFFBFBFFFGGFGFGG +@M01368:8:000000000-A3GHV:1:1106:26031:14826/1 +GATCACAGGTCTATCACCCT ++ +ABBAAFFF@FFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1106:27607:15990/1 +GACAGATACTGCGACATAGG ++ +BBBBBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 +GTATCCGACATCTGGTTCCT ++ +BBBAAFABDBBFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1107:14451:26748/1 +GTATGTTCGCCTGTAATATT ++ +BABBBFFDABBBGGGGGGGG +@M01368:8:000000000-A3GHV:1:1107:18714:24788/1 +GTATGTTCGCCTGTAATATT ++ +BBBCCFFFDEDEGGGGGGGG +@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 +ATATTGAACGTAGGTGCGAT ++ +ABCBBFFFFFAFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 +AATAAGACATCACGATGGAT ++ +BBBBBFBBFFFFGGGGCEGG +@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 +CTTCAGGGTCATAAAACCTA ++ +3AABABFFBFFFGCFGGGGG +@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 +AACTGTATCCGACATCTGGT ++ +ABBBA@FFFFBBGGGGGGGG +@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 +GCTAAAGTGAACTGTATCCG ++ +1AAAAF1B13BBBEGGGFGG +@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 +GTTATTATTATGTCCTACAA ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 +TAGGTGCGATAAATAATAGG ++ +BCCBAFFBCCCCGGGGGGGG +@M01368:8:000000000-A3GHV:1:1108:14328:14751/1 +ATAATAGGATGAGGCAGGAA ++ +BCCCBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1108:16350:22621/1 +TTAGTAAGTATGTTCGCCTG ++ +AAAACFFFFFFFGGGFGGGG +@M01368:8:000000000-A3GHV:1:1108:21791:23272/1 +CTTTAGTAAGTATGTTCGCC ++ +3ABBBFFFFF@FGGGGGGGC +@M01368:8:000000000-A3GHV:1:1108:23314:26089/1 +ATTGAACGTAGGTGCGATAA ++ +BBBBBFFF?FFAGFBFGGEG +@M01368:8:000000000-A3GHV:1:1108:25084:18127/1 +TTATTATGTCCTACAAGCAT ++ +CCBBCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1108:7626:10048/1 +ATCAAAGACAGATACTGCGA ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1109:11270:12255/1 +AATCAAAGACAGATACTGCG ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1109:19224:11655/1 +AAATAGCCCACACGTTCCCC ++ +BBB@A4FFFBBBGGGGGGGG +@M01368:8:000000000-A3GHV:1:1109:21978:9490/1 +GGGTCATAAAACCTAAATAG ++ +?A3AA?DFFFFFGGGGGGGF +@M01368:8:000000000-A3GHV:1:1109:22910:4079/1 +TAATAGGATGAGGCAGGAAT ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1109:24217:19077/1 +AAAGTGAACTGTATCCGACA ++ +>AAA3BDFFFFDGBFFEEGG +@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 +CCTGTAATATTGAACGTAGG ++ +AABBBFFFFFBBF5BGGFGA +@M01368:8:000000000-A3GHV:1:1109:27103:7153/1 +GTAATATTGAACGTAGGTGC ++ +33>A?FFFDFBFGCG4EGFG +@M01368:8:000000000-A3GHV:1:1109:8763:7859/1 +TAGTAAGTATGTTCGCCTGT ++ +BABAADFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1110:12808:18367/1 +AATTAACACACTTTAGTAAG ++ +BBBBBFFFFBBFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 +TAACACTTGGGGGTAGCTAA ++ +AAA?1CFFFFADGEEGFGB1 +@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 +CTGTAATATTGAACGTAGGT ++ +BBBBBFFFFFFFGGGGGGCB +@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 +CATTCAATTGTTATTATTAT ++ +ABBBAFFFFFFFFGGGGGGG +@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 +AAATAATAGGATGAGGCAGG ++ +BBBBBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 +ATAAATAATAGGATGAGGCA ++ +AABBBFFFFFFFCGGGCGGG +@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 +ACTTGGGGGTAGCTAAAGTG ++ +3ABB?FAB?DAAGGGFBGGB +@M01368:8:000000000-A3GHV:1:1111:13195:14562/1 +ATCAAAGACAGATACTGCGA ++ +ABAAAFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 +GCCAACACGTTCCCCTTAAA ++ +>>A3>AAAACFFFGFGFGGG +@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 +GTCCTACAAGCATTAATTAA ++ +3>3AAFFFFFAFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 +ACACACTTTAGTAAGTATGT ++ +CBCCDCFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 +TCCTACTTCAGGGTCATAAA ++ +AA3AAFFFFFFFGGGFGFFG +@M01368:8:000000000-A3GHV:1:1111:9655:13916/1 +GGCCCATAACACTTGGGGGT ++ +1>>1AAFFFFFBGGFE1EEE +@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 +GTATCCGACATCTGGTTCCT ++ +AAABABBBBBBFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 +CTGTAATATTGAACGTAGGT ++ +AABABFFFFFFFF4GGGGEB +@M01368:8:000000000-A3GHV:1:1112:9832:6701/1 +CGCCTGTAATATTGAACGTA ++ +AABBBBFFFFFFGGGFGGGG +@M01368:8:000000000-A3GHV:1:1113:13532:8618/1 +GGAATCAAAGACAGATACTG ++ +AA?AAFFFFFFFGGGGGFBF +@M01368:8:000000000-A3GHV:1:1113:21679:18011/1 +GATCACAGGTCTATCACCCT ++ +AA>1>11F1D1DDFFFFGGG +@M01368:8:000000000-A3GHV:1:1113:25528:14016/1 +CCTACAAGCATTAATTAATT ++ +>AAAAFFFBFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1113:5741:16959/1 +TAGTAAGTATGTTCGCCTGT ++ +ABBBBFFFFFFFGGGFGGGG +@M01368:8:000000000-A3GHV:1:1114:10130:11959/1 +CGTTCCCCTTAAATAAGACA ++ +>A33>FFFFFF@FFFGGGGF +@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 +CTTTAGTAAGTATGTTCGCC ++ +AABCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1114:15066:16302/1 +TAAATAAGACATCACGATGG ++ +BBBBAFFBDFBFBGGGGGFG +@M01368:8:000000000-A3GHV:1:1114:16639:15258/1 +CCTAAATAGCCCACACGTTC ++ +11>>ABFFFFFFGGCGC1BB +@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 +TCCTACAAGCATTAATTAAT ++ +CCCCCFFFFCFFGGGGGGGG
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/paired_hardtrimmed5_pair2_.fastqsanger Fri Oct 08 09:57:52 2021 +0000 @@ -0,0 +1,396 @@ +@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 +ATCTGGTTCCTACTTCAGGG ++ +BCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 +GTTATTATTATGTCCTACAA ++ +AAAAAFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 +GTATCCGACATCTGGTTCCT ++ +CCCCCFDDDDDFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 +ATTAATTAACACACTTTAGT ++ +33AA?DFD5BDFGGGFEBDG +@M01368:8:000000000-A3GHV:1:1101:5446:12248/1 +AATTAACACACTTTAGTAAG ++ +CCCCDFFFFCCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1101:5861:6452/1 +ATTATGTCCTACAAGCATTA ++ +ABCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:10403:6021/1 +CGCTCCGGGCCCATAACACT ++ +>A@AAAAD2ADDFFGGGGGF +@M01368:8:000000000-A3GHV:1:1102:10677:23253/1 +CCTTAAATAAGACATCACGA ++ +ABBBBFFFFFFFGGGGGGGC +@M01368:8:000000000-A3GHV:1:1102:13809:1733/1 +ATATTGAACGTAGGTGCGAT ++ +BCCBCFFFFFBFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:17584:10050/1 +ATCAAAGACAGATACTGCGA ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:18842:24844/1 +CACTTGGGGGTAGCTAAAGT ++ +BBBCBFF@CCBBGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:21788:11027/1 +GCCCACACGTTCCCCTTAAA ++ +ABCCBCCBBFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:5728:8777/1 +ATATTGAACGTAGGTGCGAT ++ +BBBBBFFFFFAFGG5GGGGG +@M01368:8:000000000-A3GHV:1:1102:5983:21093/1 +GTATCCGACATCTGGTTCCT ++ +AAABCFCABBCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1102:7765:18353/1 +CTCCGGGCCCATAACACTTG ++ +CCDCCCCCCBCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1103:10405:17879/1 +GTATCCGACATCTGGTTCCT ++ +CBCCCFCCCCCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1103:13493:8205/1 +GTAGCTAAAGTGAACTGTAT ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1103:14060:21273/1 +GTATCCGACATCTGGTTCCT ++ +AAABAFCCCCCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1103:14528:12993/1 +GCCCATAACACTTGGGGGTA ++ +BBBBBFFFBDFFGFBCG2EG +@M01368:8:000000000-A3GHV:1:1103:17657:20856/1 +AAAGCGAACTGTATCCGACA ++ +ABB?AABBBBFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1103:23099:3794/1 +AAGACAGATACTGCGACATA ++ +BBBBBFFFFFFFGGEEGGFG +@M01368:8:000000000-A3GHV:1:1103:25465:20010/1 +GACATAGGGTGCTCCGGCTC ++ +ABBBBFFFFBAAGGGFEFGG +@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 +AAATAAGACATCACGATGGA ++ +3A3AAFFFFBFFGGG2CF?4 +@M01368:8:000000000-A3GHV:1:1103:6074:6549/1 +TAACACTTGGGGGTAGCTAA ++ +CCCBCFFFFFCCGGGGGGGG +@M01368:8:000000000-A3GHV:1:1104:11572:8264/1 +GTATCCGACATCTGGTTCCT ++ +BABCBFBACCCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1104:11928:24197/1 +ACGTAGGTGCGATAAATAAT ++ +CDDCCFCFFFBCGGGGGGGG +@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 +CACTTGGGGGTAGCTAAAGT ++ +ABA?AFBAB2>AFGGGGFFG +@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 +CACGATGGATCACAGGTCTA ++ +AAABABCAFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 +ATTCAATTGTTATTATTATG ++ +AAAABFFFFFFFGGGGGGGC +@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 +AGCTAAAGTGAACTGTATCC ++ +AAA?AFFF3DFFFGGBGFF3 +@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 +CTACAAGCATTAATTAATTA ++ +BBBBBFFBFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1104:7756:4776/1 +GGCCCATAACACTTGGGGGT ++ +CCCCCCFFFFFFGGGGGGGE +@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 +ATAAATAATAGGATGAGGCA ++ +CCCCBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 +ATTGAACGTAGGTGCGATAA ++ +CDDDDFFFBFFCGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 +TTAGTAAGTATGTTCGCCTG ++ +AA1A1DFFFFFBGG3A111F +@M01368:8:000000000-A3GHV:1:1105:15502:11453/1 +GTCCTACAAGCATTAATTAA ++ +A3AAAFFFFFCFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:16787:9771/1 +GTTATTATTATGTCCTACAA ++ +AABCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:17564:15723/1 +TATGCTCGCCTGTAATATTG ++ +EDCCDFFCEEDCGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:18108:12091/1 +TAATAGGATGAGGCAGGAAT ++ +CCCCCFBFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:18331:16683/1 +TCTGGTTCCTACTTCAGGGT ++ +BABA?DFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:20577:18421/1 +GGGGGTAGCTAAAGTGAACT ++ +ABB@3>AAFFFFFEAGGGFG +@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 +TAGCTAAAGTGAACTGTATC ++ +CBCBBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 +TTATGATTATGTCCTACAAG ++ +BAAAAFFFBFFFGGGGGGFF +@M01368:8:000000000-A3GHV:1:1105:7169:17145/1 +AACCTAAATAGCCCACACGT ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1106:15481:16176/1 +CTTTAGTAAGTATGTTCGCC ++ +ABBBBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1106:17389:18671/1 +CCATAACACTTGGGGGTAGC ++ +3>>A@FFFFFFFGGGFEECF +@M01368:8:000000000-A3GHV:1:1106:22927:3863/1 +GTATCCGACATCTGGTTCCT ++ +A>ABAFA?D@AFGGGGFGGG +@M01368:8:000000000-A3GHV:1:1106:23625:4236/1 +TAGCTAAAGTGAACTGTATC ++ +@?AAAFFBFBFFFGGFGFGG +@M01368:8:000000000-A3GHV:1:1106:26031:14826/1 +GATCACAGGTCTATCACCCT ++ +ABBAAFFF@FFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1106:27607:15990/1 +GACAGATACTGCGACATAGG ++ +BBBBBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 +GTATCCGACATCTGGTTCCT ++ +BBBAAFABDBBFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1107:14451:26748/1 +GTATGTTCGCCTGTAATATT ++ +BABBBFFDABBBGGGGGGGG +@M01368:8:000000000-A3GHV:1:1107:18714:24788/1 +GTATGTTCGCCTGTAATATT ++ +BBBCCFFFDEDEGGGGGGGG +@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 +ATATTGAACGTAGGTGCGAT ++ +ABCBBFFFFFAFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 +AATAAGACATCACGATGGAT ++ +BBBBBFBBFFFFGGGGCEGG +@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 +CTTCAGGGTCATAAAACCTA ++ +3AABABFFBFFFGCFGGGGG +@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 +AACTGTATCCGACATCTGGT ++ +ABBBA@FFFFBBGGGGGGGG +@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 +GCTAAAGTGAACTGTATCCG ++ +1AAAAF1B13BBBEGGGFGG +@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 +GTTATTATTATGTCCTACAA ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 +TAGGTGCGATAAATAATAGG ++ +BCCBAFFBCCCCGGGGGGGG +@M01368:8:000000000-A3GHV:1:1108:14328:14751/1 +ATAATAGGATGAGGCAGGAA ++ +BCCCBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1108:16350:22621/1 +TTAGTAAGTATGTTCGCCTG ++ +AAAACFFFFFFFGGGFGGGG +@M01368:8:000000000-A3GHV:1:1108:21791:23272/1 +CTTTAGTAAGTATGTTCGCC ++ +3ABBBFFFFF@FGGGGGGGC +@M01368:8:000000000-A3GHV:1:1108:23314:26089/1 +ATTGAACGTAGGTGCGATAA ++ +BBBBBFFF?FFAGFBFGGEG +@M01368:8:000000000-A3GHV:1:1108:25084:18127/1 +TTATTATGTCCTACAAGCAT ++ +CCBBCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1108:7626:10048/1 +ATCAAAGACAGATACTGCGA ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1109:11270:12255/1 +AATCAAAGACAGATACTGCG ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1109:19224:11655/1 +AAATAGCCCACACGTTCCCC ++ +BBB@A4FFFBBBGGGGGGGG +@M01368:8:000000000-A3GHV:1:1109:21978:9490/1 +GGGTCATAAAACCTAAATAG ++ +?A3AA?DFFFFFGGGGGGGF +@M01368:8:000000000-A3GHV:1:1109:22910:4079/1 +TAATAGGATGAGGCAGGAAT ++ +CCCCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1109:24217:19077/1 +AAAGTGAACTGTATCCGACA ++ +>AAA3BDFFFFDGBFFEEGG +@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 +CCTGTAATATTGAACGTAGG ++ +AABBBFFFFFBBF5BGGFGA +@M01368:8:000000000-A3GHV:1:1109:27103:7153/1 +GTAATATTGAACGTAGGTGC ++ +33>A?FFFDFBFGCG4EGFG +@M01368:8:000000000-A3GHV:1:1109:8763:7859/1 +TAGTAAGTATGTTCGCCTGT ++ +BABAADFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1110:12808:18367/1 +AATTAACACACTTTAGTAAG ++ +BBBBBFFFFBBFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 +TAACACTTGGGGGTAGCTAA ++ +AAA?1CFFFFADGEEGFGB1 +@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 +CTGTAATATTGAACGTAGGT ++ +BBBBBFFFFFFFGGGGGGCB +@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 +CATTCAATTGTTATTATTAT ++ +ABBBAFFFFFFFFGGGGGGG +@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 +AAATAATAGGATGAGGCAGG ++ +BBBBBFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 +ATAAATAATAGGATGAGGCA ++ +AABBBFFFFFFFCGGGCGGG +@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 +ACTTGGGGGTAGCTAAAGTG ++ +3ABB?FAB?DAAGGGFBGGB +@M01368:8:000000000-A3GHV:1:1111:13195:14562/1 +ATCAAAGACAGATACTGCGA ++ +ABAAAFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 +GCCAACACGTTCCCCTTAAA ++ +>>A3>AAAACFFFGFGFGGG +@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 +GTCCTACAAGCATTAATTAA ++ +3>3AAFFFFFAFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 +ACACACTTTAGTAAGTATGT ++ +CBCCDCFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 +TCCTACTTCAGGGTCATAAA ++ +AA3AAFFFFFFFGGGFGFFG +@M01368:8:000000000-A3GHV:1:1111:9655:13916/1 +GGCCCATAACACTTGGGGGT ++ +1>>1AAFFFFFBGGFE1EEE +@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 +GTATCCGACATCTGGTTCCT ++ +AAABABBBBBBFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 +CTGTAATATTGAACGTAGGT ++ +AABABFFFFFFFF4GGGGEB +@M01368:8:000000000-A3GHV:1:1112:9832:6701/1 +CGCCTGTAATATTGAACGTA ++ +AABBBBFFFFFFGGGFGGGG +@M01368:8:000000000-A3GHV:1:1113:13532:8618/1 +GGAATCAAAGACAGATACTG ++ +AA?AAFFFFFFFGGGGGFBF +@M01368:8:000000000-A3GHV:1:1113:21679:18011/1 +GATCACAGGTCTATCACCCT ++ +AA>1>11F1D1DDFFFFGGG +@M01368:8:000000000-A3GHV:1:1113:25528:14016/1 +CCTACAAGCATTAATTAATT ++ +>AAAAFFFBFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1113:5741:16959/1 +TAGTAAGTATGTTCGCCTGT ++ +ABBBBFFFFFFFGGGFGGGG +@M01368:8:000000000-A3GHV:1:1114:10130:11959/1 +CGTTCCCCTTAAATAAGACA ++ +>A33>FFFFFF@FFFGGGGF +@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 +CTTTAGTAAGTATGTTCGCC ++ +AABCCFFFFFFFGGGGGGGG +@M01368:8:000000000-A3GHV:1:1114:15066:16302/1 +TAAATAAGACATCACGATGG ++ +BBBBAFFBDFBFBGGGGGFG +@M01368:8:000000000-A3GHV:1:1114:16639:15258/1 +CCTAAATAGCCCACACGTTC ++ +11>>ABFFFFFFGGCGC1BB +@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 +TCCTACAAGCATTAATTAAT ++ +CCCCCFFFFCFFGGGGGGGG
--- a/test-data/sanger_full_range_report_results1.txt Tue Jul 30 06:26:49 2019 -0400 +++ b/test-data/sanger_full_range_report_results1.txt Fri Oct 08 09:57:52 2021 +0000 @@ -3,21 +3,24 @@ ========================== Input filename: input_1.fastq Trimming mode: single-end -Trim Galore version: 0.6.3 -Cutadapt version: 2.4 -Number of cores used for trimming: 1 +Trim Galore version: 0.6.7 +Cutadapt version: 3.4 +Python version: could not detect +Number of cores used for trimming: 4 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 +Unable to auto-detect most prominent adapter from the first specified file (count Nextera: 0, count smallRNA: 0, count Illumina: 0) +Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior). Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection)) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length before a sequence gets removed: 20 bp -This is cutadapt 2.4 with Python 3.7.3 -Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq -Processing reads on 1 core in single-end mode ... -Finished in 0.00 s (1608 us/read; 0.04 M reads/minute). +This is cutadapt 3.4 with Python 3.9.6 +Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq +Processing reads on 4 cores in single-end mode ... +Finished in 0.01 s (5282 µs/read; 0.01 M reads/minute). === Summary === @@ -31,10 +34,10 @@ === Adapter 1 === -Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times. +Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times No. of allowed errors: -0-9 bp: 0; 10-13 bp: 1 +1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 0.0%
--- a/test-data/sanger_full_range_report_results1gz.txt Tue Jul 30 06:26:49 2019 -0400 +++ b/test-data/sanger_full_range_report_results1gz.txt Fri Oct 08 09:57:52 2021 +0000 @@ -3,11 +3,14 @@ ========================== Input filename: input_1.fastq.gz Trimming mode: single-end -Trim Galore version: 0.6.3 -Cutadapt version: 2.4 -Number of cores used for trimming: 1 +Trim Galore version: 0.6.7 +Cutadapt version: 3.4 +Python version: could not detect +Number of cores used for trimming: 4 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 +Unable to auto-detect most prominent adapter from the first specified file (count Illumina: 0, count Nextera: 0, count smallRNA: 0) +Defaulting to Illumina universal adapter ( AGATCGGAAGAGC ). Specify -a SEQUENCE to avoid this behavior). Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; default (inconclusive auto-detection)) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp @@ -15,10 +18,10 @@ Output file will be GZIP compressed -This is cutadapt 2.4 with Python 3.7.3 -Command line parameters: -j 1 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq.gz -Processing reads on 1 core in single-end mode ... -Finished in 0.02 s (7871 us/read; 0.01 M reads/minute). +This is cutadapt 3.4 with Python 3.9.6 +Command line parameters: -j 4 -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC input_1.fastq.gz +Processing reads on 4 cores in single-end mode ... +Finished in 0.01 s (5217 µs/read; 0.01 M reads/minute). === Summary === @@ -32,10 +35,10 @@ === Adapter 1 === -Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times. +Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 1 times No. of allowed errors: -0-9 bp: 0; 10-13 bp: 1 +1-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 0.0%
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/trimmed_polyA_reads_pair1.fastqsanger Fri Oct 08 09:57:52 2021 +0000 @@ -0,0 +1,396 @@ +@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 +ATCTGGTTCCTACTTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT ++ +BCCCCFFFFFFFGGGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB +@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 +GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT ++ +AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A// +@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF. +@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 +ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG ++ +33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/A<C@EGFDCGGGHFHHHEGFGHBFHG0:CEHFCHGGED.;0CEF.F99B0CFFEEFGGG0FBFBBF0F/FFBDE?/9//9B.FFBFFFFFFBF..A..;@B-- +@M01368:8:000000000-A3GHV:1:1101:5446:12248/1 +AATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTAT ++ +CCCCDFFFFCCFGGGGGGGGFGHHHHHGGGGHHHHHHHHHHHHHHHHGBGHGGHGGHHHHHHHHHHGHGHGGGGGHHHHHHHHGHHHHHHHHHGGGGGHHHHFFGHHHGGGGGGHHHGFGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGGHHHHHHHHHHHHHFHGGGHHHHGGGGGG:FE;EGEGGGGG/;?FGGGGGGGFFFFGGFFFFFFFFFBFFFFFFFFFFBFFFFFFEFFFFFEFFF +@M01368:8:000000000-A3GHV:1:1101:5861:6452/1 +ATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTT ++ +ABCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHGHHHHGHHHHHHHHHHHGGGGHHHHHHHHFHHHHHHGGHGHGGHGGHHHHHHHGGHFHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHHEGGHHGGGGGGHHHGGGGHGGGGGHHHHHHGGGDCGHHHHGGGGGGGHEFGGGGHGHHHGHGGGFGGGGGGGEGGGGGGG?E0CEFGGGGGFEE9EEFFFFFBFFFFFFFBFFBD.AFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1102:10403:6021/1 +CGCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTG ++ +>A@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE<CG1<GF0/DD000=<DHGBDFDCECE/:AC?-;-;9B/ABBB-AD9BFB99AB?BDFBAD-.9..@;=;;..9..9/9;BEF;A:9/BFF +@1:A:M01368:8:000000000-A3GHV:1:1102:10677:23253/1_PolyA:1 +CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCTGTCTCTTATACAC ++ +ABBBBFFFFFFFGGGGGGGCGGGHHHHHGHHHHHHHHHGHHHHHHHHHHHGHHHHHGGGGGHGHHHHHHHHHHHHHBGFHHHHGHGHGGHGGGCGGGHHHGGGGGGGHHHGHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFDFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFEDBDFFFFFFFEFFFE0F0FBFFFF0FFFFFFFFFFFFFFFFF +@M01368:8:000000000-A3GHV:1:1102:13809:1733/1 +ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGAGGGCTATTTAGGTTTTATGCTGTCTCTTATACACATCTCCG ++ +BCCBCFFFFFBFGGGGGGGGGGHHHHHHHHHHHGHHGGGGGH3EHHHGHGHHHHHHHHHGGCEGGGHHHGGGFHGGGGGGHHGEAEFHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGCFFGHHFGHHHGHHHHHHCHGFHHHHGGHGGG00EHGHHHGFHHGEHGHFFFHHGGFHHHGGGGGGGGGB;;CFFGGFGFFBFFDFED;E.:A?DFFFFFFFF:FFFFF0BFFBBBFFFFFBFF0BFBF9F. +@M01368:8:000000000-A3GHV:1:1102:17584:10050/1 +ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT ++ +CCCCCFFFFFFFGGGGGGGGGGGHHHHHGGHHHGGGGGGHHHGFGDHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGEGGHHHHHHHGHGHHGHHHHGHHHHGGGGFDGHFHGEHHHHHHGFFHHHHHHHHHHHHHHHHHGGHHHHHHHHHHFHHHHHHGGGGGHHHHGGGHHGHHHGGGGGGGGGGGFGFFGGGGGGGCFGGGFGGGGGGGFFFFFFFFFFFFFFFFFFF;.BF9ADF/9;A;DFFF0 +@M01368:8:000000000-A3GHV:1:1102:18842:24844/1 +CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCT ++ +BBBCBFF@CCBBGGGGGGGGGGHHGHGGHHHGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGGHHHHHHHHHHHHHHHHHHHHGGGHGGHHHHHHGHBFGHHHHHHHHBGHHHHHHGHGGGHFGCGGFHHFHFFHHBHHHHFFHFHHHHGGDHGGBC?;@DFBFFFFFFFFFBFFFFFFFFFFFFFF.B?FB.@;;DFFFFFFFFFFFEE-A./BBBFBFBFFF//BFB/BFF/ +@M01368:8:000000000-A3GHV:1:1102:21788:11027/1 +GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCTG ++ +ABCCBCCBBFFFGGGGGGGGGGGHGHHHHHHGGHGGHHHHHHHHGHHHHHHHHHHHHHHHHHHGHHHHHFGGGGHHHHHHHHHHHHHHHHHHHHHHGHGHGGFCGGCGGGHHHGGGGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFDFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEF9EFFFFFFFFFFFBFF0FFBBBBFBFBFFFF0 +@M01368:8:000000000-A3GHV:1:1102:5728:8777/1 +ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG ++ +BBBBBFFFFFAFGG5GGGGGGGHHHGHHHHHHHHHHGGGGGHHHHHHGHHHHHHFGHHHGGGGGFHGHGGGHHHGGGCEGHHGG?F1BGGGGGHHHHHGGGGEGGHHHHGGGGGCGHGGGGCGHHHHHHHHHHHHHHHHGGHHHHGGCEEDFDEGGHHHHHHHCEHHFHHHHGHGFHHHFFGGGFFFGGFGBGFFFFFFFFGFDGGD9EFA.DAFA/;FEFF9FB?/BBF/FFFFBBFFFFF/FFEFFFBB +@M01368:8:000000000-A3GHV:1:1102:5983:21093/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +AAABCFCABBCFGGGGGGGGGGHHHHHHGFHHHHHHHHGGHHHGHGHHHGGGGFHHHHHHHHGHHHHHHHHHHHHHGGHGGHHHHHHFHGGHHHHFHGHHEHHHHHHHGHHHHHD@FGGHHHHHFFFHHGGHGH?DHHHHHGHGHEGG/@?ADGFGGFFFFAFFFFFFFFFFFFFFFFFFFFFFFAAFFFFFFDFFFFFFFF;D9BFFFFFFFFFFFFFFFFFEFFFBBFFF0BFFFBBFF0EFFFFFFFA +@M01368:8:000000000-A3GHV:1:1102:7765:18353/1 +CTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTC ++ +CCDCCCCCCBCFGGGGGGGGGGGGGGGHHHHHHDHHHHHHHHHHHGGGGGHHGHGHHHGHHHHHHFHHHHGHHHHHHGHGHHHGHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHGFFHHHHHGHHHHHHHHHHHGHHHHHGCFGGHGHFHHGHGHGHHHHGGFHHHHGGGHGBFGCC=BFFFFFFFF-@DFFFFFFFFFFFFFFFFFFFFFFAFFAEF.::FBFBFFE-A9:BFFFF0 +@M01368:8:000000000-A3GHV:1:1103:10405:17879/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +CBCCCFCCCCCFGGGGGGGGGGHHHHHHHHHHHHGHHHGHHHHHHHHHHHGGGGGGHHHHHHHHHHHHHHHHHHHHGGHGGHHHGHHGHHGHHHHHHHHHCGGFHHHHGHHHHHGCFGGHHGHHHHHHGFHHHGHHHHHHHGHGHGFF/CC?ADAFGFFFFFFFFFFFFFFDFFFFFFFFAFFFFFFFFFFFEDFFFFFFFFFFAFFFFFFFFFFFFFFFFFFFFFFBBFBFBFFFFFFFFFFFFFFFFF? +@M01368:8:000000000-A3GHV:1:1103:13493:8205/1 +GTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT ++ +CCCCCFFFFFFFGGGGGGGGGGGGGGGHFHHHHHHHHHHHHHHHHGHHHHHHHHGHHHIHHHHHHHGGGGFHHHHHHHHHHHHHHHHHHHGHGGHGGHHHHHHHHHGHHHHHHHHHHHHHHHHHGHHHH/FGGGGHHHHHHHGGEHHHHHGHHHHHHGHGHEFEFGGFFFFFFFFFFFFFFFFFFFFFAFDFFFFFAFFFF;;@FFEFFFFFFFFFFE=?AFFFFFFFFFFFFFFFFFFBFBBBFFFFEFF +@M01368:8:000000000-A3GHV:1:1103:14060:21273/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTACTGTCTCTTATACAC ++ +AAABAFCCCCCFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHGHHHHGGGGGFHHHHHHHHHHHHHHHGHHHHGGHGGHHHHHHHHHHHHHHHHHH?GGHHHHHHGHHHHHFEGGGHHHHHHHHHHHHHHHGFHHHFHGHGHGFGCGG@@BCFGFFFFFFF?FFFFFFFFAFFFAD?@FFEEFFFFFFFFFFFFFFFFFFD9BFF/B/FFF/:/9FB9/BFA//;BFB:FFFBB/BFFFFFBFFFFB/ +@M01368:8:000000000-A3GHV:1:1103:14528:12993/1 +GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT ++ +BBBBBFFFBDFFGFBCG2EGFFGHHCF5AFHHHFHFHHHGG?CEHBHHDGFHHGHHGHBGHABFFBGHGDGGGFHGHHGGHHHGGG@E1GEHHHGHHFHH3FEDHHHHGFGHGFHHGHFBGA?FGHHHHHGHHFFGHFDFGGHHH2??<ECFHHGHHHHHHHHHGHH<1DFGHGC<.FCB/<=@DCFFFFFEDA=-AAB9B/9B9B-@?.-;A-AFF.=-=BFEFEDEFFFBFEA-A.B//9F9F//;BBB +@M01368:8:000000000-A3GHV:1:1103:17657:20856/1 +AAAGCGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTAT ++ +ABB?AABBBBFFGGGGGGGGGGHHHHHHHHGHHHHHHHHHHHHHHHHHGHHHHHHGHHHHGGGGGHHHHHHHGHHFHHHHHHHHHHGGGGGHHHHHHHH3BGGHHHHHHHEHHHHHHHGHHHHGEEGGGHHHHHHHHGHHFHGHGHGHGHHCHGGECCDFGB@AFFFFFFFFFFFFFFFFFDAFFFFFFFFFFFFFCAF;AFFFFEFFBBEFFF9FBBFFFFFFFFFFEFFFFFFFFFFFFF/FFFFBFF9 +@M01368:8:000000000-A3GHV:1:1103:23099:3794/1 +AAGACAGATACTGCGACATAGGGTGTTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCTCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGTCT ++ +BBBBBFFFFFFFGGEEGGFGGGGCFGEHCGGGGGGFHFFEDEHFGGDEHHHHHHGGFECGHHHHGFEFGCGHGGFGGGHBGFH3/BF3GGGHHEEEHHG22???BCFFFFFFHHFHFF@FGHH11GHHHHFGFHHHHGGHHHHEFFGFGHFG1DGFGGGGEDCCCAEGGFFHHFHHHHHHCFBBFGBFFF/0;B0B00;B0BBFGBB9BFGEFGGGFFDEEAB;BDDBFBFFFFF@;.9BF..;-./-A=F +@M01368:8:000000000-A3GHV:1:1103:25465:20010/1 +GACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATCGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAACAGAC ++ +ABBBBFFFFBAAGGGFEFGGGGHHFEFFHFGGGGGHHHGHFGGGGGHHHGGGGGGGGHGEEEGGHHHGFHEHFHDGHGHEHGF?FGG?GGGGHHGHF4F?2?DDCDHGHHHHHFG2@FGHHHGH?CFGGHFHFHHHHGDDGGHGGGGGHGFH.<AFAGHHHFHGHHFCGFHHHHHGHHHHBGHHGGDGAGF.CEGCFDGGGGGEFFF=B.FFFEFFFFFFADF?/9//BFF.B/;:>---;--..;.../9 +@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 +AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT ++ +3A3AAFFFFBFFGGG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/<A@DFBBEGC--:;GHHHGBECG@:9-9.-9C/ABB?B@?.EDAFFFFBAFBFBFBFFFFF//;BBFFFF/;/9BBF/BBFBFFFFFFFFFEFF??BF/.AF./;B/9FFFFFFBFBFFFBFFFBFFFFF/BF/A---9.9A-B?DFFF/B/B?---.//;. +@M01368:8:000000000-A3GHV:1:1103:6074:6549/1 +TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG ++ +CCCBCFFFFFCCGGGGGGGGGG5GFHHHHHGHHHGGGGGHHHGHHHHHHHHHHHHGHHHHHHHHHGGHHHHGHHHHHHGGGGHHHHHHHHHHHHHHGHHHHHHHGGHGGHHHHHHHFGHHGHGHHHHHHHHHHHHHGHHHHHGGGGCHHHHEHGHHHEGHHHFGGHHHHGHGHGHF@CCBDFAFFFFFF-CDFFFFFFFFFFFFFAFFFFFEFF;BFF?FEDFFFFFFFF-A./FFFFFFFFFFFF/BFF/ +@M01368:8:000000000-A3GHV:1:1104:11572:8264/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +BABCBFBACCCFGGGGGGGGGGGHHGHHGHHHHGFGCHGHHGFFHEHHHHGGGGFGHHHHHHHHHHHHGHHHHHHHGGGGGHHHFFHHGHHHHHHHHHHHEHHHHHHHGHHGHGGEGEGHHHFHHHHGHHHHHHGGHHHHHGHGEGEHCGG-BGDFGFFFFAFFDFFFFEFFCFFDDFFFFFFFEC>;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9 +@1:A:M01368:8:000000000-A3GHV:1:1104:11928:24197/1_PolyA:1 +ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGAT ++ +CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D +@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 +CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT ++ +ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9 +@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 +CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC ++ +AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.- +@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 +ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC ++ +AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB +@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 +AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT ++ +AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB +@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 +CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG ++ +BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF +@1:A:M01368:8:000000000-A3GHV:1:1104:7756:4776/1_PolyA:1 +GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG ++ +CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF +@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 +ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT ++ +CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0 +@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 +ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC ++ +CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 +TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11</?//<@?/F<<<A-<FBGH0.00=G0<<C//./0;;GC....<C.=.00;;BB./:.900////.C000;0090;..//000;C/;;/B/9BA9A9-AEEFF?B---;B99//9/;A//9 +@M01368:8:000000000-A3GHV:1:1105:15502:11453/1 +GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCTGTCTCTTATACACATCTC ++ +A3AAAFFFFFCFGGGGGGGGGGHHGHHHGHHHHHHHHHFGHHHHHGGGGHGHHHHHGFHHHHGHGGGHHGGHGGHHHHHHHHHHHHHHGGGGGFHGHHHGHHHHHGHHHHGGGGGGHGEGFECHEHGGGGGGHHHGADFHHGGGGHHHHH<ECGGGGHHHHGGGGGGGHGGGGGGHHHGHGHHGEHBFFFGFGEGGGGGEDEGF0C;;BFFFGGGBEFFE/FFFFFFFFFFFFFFEFFFFFFFFBFFFBFF +@M01368:8:000000000-A3GHV:1:1105:16787:9771/1 +GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT ++ +AABCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIHHHHHDHHHHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGGHHHHHGGGGGGHHGHGGGGGGGEEGEGFGHHHGGGGFFGGGGGGGGGGGGGGGEG?G;:EFFFFFFFFF:AEFFFFFFFFBFFFFFFFFFEFF/ +@2:A:M01368:8:000000000-A3GHV:1:1105:17564:15723/1_PolyA:2 +TATGCTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG ++ +EDCCDFFCEEDCGGGGGGGGGGHHHHHGFGHGGHGGHHHHHHHHGHHEHHGGGGGHHHHHHHHHGHHHGHHHHGGGGGHHHHFFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHFEHHHHHHHHHHGHHGHGGHGGGCGGGGHHH1FGGGFGGFGGGGGGGGGGEFGGGGGGGGGGGGGGGGGFFFFFFFFFFAEFFFFFFFFFFBBFBBFFFFFE1:BFF +@M01368:8:000000000-A3GHV:1:1105:18108:12091/1 +TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACAC ++ +CCCCCFBFFFFFGGGGGGGGGGHHHHHHHHHHHHHGGGGGGFHHHBFGHHHGGGGGGHHGGGGGHHGFGGHHHHHHGGGGGGHGHFGGGGGGDGEGEFGCHHHHHHHHHHGHHHHGEDHHHGGGCDGGGGFHFHGHHHGHHGHFGHHHFHHGFHHHFHHGGHFHFHHGB0CGHGFHCBGGGGEDEE9ADFEFFFGFBFGFFGGFFFFFGGFGFFFFFFFF/A?FEFFFFFEF-A.B//;BFFEF//BB/// +@1:A:M01368:8:000000000-A3GHV:1:1105:18331:16683/1_PolyA:1 +TCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT ++ +BABA?DFFFFFFGGGGGGGGGGHHHHHHGHGHHGGGHHGHGGGGFFHHGHHHGHHHHHHH3FGHHGGGHGGHHHHFHHHFHGHHHGHHHHHHHHHHHHGHHHHGGCGGGHHGHHHHHHHHHHHHHFHHGHHGHGHEFGGGGABFFFGBEGGAFFFFFFFFFFFFFDDFF?DFFFFFFFFAEFFFFFFFFFFFFB;9FFFFFEFFFFFFFFFFFF?FFFFFFF/BFFFFFFFB.DABFFFFFFB.//:FB/ +@M01368:8:000000000-A3GHV:1:1105:20577:18421/1 +GGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCC ++ +ABB@3>AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/: +@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 +TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTT ++ +CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF0 +@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 +TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG ++ +BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD<EHHGGGGGHHGHH?EDCCDHHHHDGFFGGGHGGFCGGHHHHFG.CFGGGGGGGGGGGBFGDEG.AFFG;AFFFFEB?/A9BBBFFFFFFF/9FFFFBDEFFFF +@M01368:8:000000000-A3GHV:1:1105:7169:17145/1 +AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGT ++ +CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHBHHHHHHHGGHGGHHHHHHFHHHHHHHHHHHHHHHHHHHHGHHHHHGGGGCHHHHHHHHGHHHHHHHFHHHHHGHGHGHHGGGGGFGGHHGGGGGGGGGGGGEGGFGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFDFFFFFFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFF-9@DDBFFFFFFFFBBFFF0 +@M01368:8:000000000-A3GHV:1:1106:15481:16176/1 +CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTT ++ +ABBBBFFFFFFFGGGGGGGGGGHHGHGHHHGHHHFGHGFGHFEHGGHGGHHHGHHHHFGHGGFGFFHHFHHHHEGHFHHHHHFGGGGGHGGGHGFHGHGGGGGGHHHGGGGHHGGGGHHHGGHGGGGGGHHHHGGGGGCFHGGGCDGHGHHHFHEHHHHHHHHHEEFHHGG:C:?0<EFGBFGGGGGFE.BFGEFFFGFFGGGGGGFDEF/FFGFFFFFFF;BBFA;.:EFFE:AAFEF.BFFFFF;9FF. +@M01368:8:000000000-A3GHV:1:1106:17389:18671/1 +CCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGGCTTTGATTC ++ +3>>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE</ECDFHHBF1DGBFBFGFH<1GHGDGGFDFE0/<--;-9./BBED;BFBD/BF9BFFFDBFDADD;FFF?->;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/ +@2:A:M01368:8:000000000-A3GHV:1:1106:22927:3863/1_PolyA:2 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT ++ +A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9/ +@1:A:M01368:8:000000000-A3GHV:1:1106:23625:4236/1_PolyA:1 +TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT ++ +@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHG<C???B=@ABBFEF@=-AFFFFFFBFAFFDFFFFA?AFFCFFABEFFFFFFFFF?FFFDFFBFF;FFEFFEFFFFFFFAF///9FF/BB +@1:A:M01368:8:000000000-A3GHV:1:1106:26031:14826/1_PolyA:1 +GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGTCTTCTGCTTG ++ +ABBAAFFF@FFFGGGGGGGGGGHHHHHHGHHHHHGGGGGHHHHHHHHGHGHHHHHHHHHHHGHGHFHFGE?CGDGGHHGGGGGGGHHHHGHGGGGGGFGGGHGHGGGGGGGGGGGGGGGGGGDGAFFGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFB/9FFBFFFFFFFFFFFFFD@D=BEFFF;FDDB////B=CF-.;BAFFFFFF;;BFE/BFFFFB +@1:A:M01368:8:000000000-A3GHV:1:1106:27607:15990/1_PolyA:1 +GACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGACTTCTGCTTGAAAAAAAAAAACTACAACCCACGGACGC ++ +BBBBBFFFFFFFGGGGGGGGGGGGHHHGGGGGGHHHGGGGGFCEGGHHHGHHGGGEGGHHHAFGGGGGGAFG//EGHHHHCGEHHHHEEHHFHGHFHHGGEF/FGGC?FDFH2DHHFFGGFGHHHHGGHHFGHHGHGHHGFHDHBGBBGHHFHHGGCCDC<=D<DGHHC;CGFCCFBECDG?FGGA-;EB9FBGFFBBF>DFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9-- +@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +BBBAAFABDBBFGGGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9 +@1:A:M01368:8:000000000-A3GHV:1:1107:14451:26748/1_PolyA:1 +GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG ++ +BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9 +@1:A:M01368:8:000000000-A3GHV:1:1107:18714:24788/1_PolyA:1 +GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG ++ +BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF +@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 +ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG ++ +ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9 +@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 +AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG ++ +BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A// +@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 +CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT ++ +3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/ +@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 +AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC ++ +ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC- +@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 +GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC ++ +1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//</ECCC>1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9 +@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 +GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT ++ +CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/ +@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 +TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC ++ +BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/<GHHFGBGFHFBGHHHGHHGEFHFHFGBF0BFGGGFGGGGGEFFF;A?EEG?CFFFFF0:BEFFFFFBFFFFFF0FFEFF?EFFFF0BFFFFBBB00 +@1:A:M01368:8:000000000-A3GHV:1:1108:14328:14751/1_PolyA:1 +ATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATAC ++ +BCCCBFFFFFFFGGGGGGGGGGHHHHHHHGHGHHHHHGGGGGFHHHFFDGHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHGHGGGGGGGGGGGCFGHHHHHHHHHHHHHHHHHGHHHHGGGDGGFGGFGGHHHHHH>FHHHFHGHGHHGHHHGHHBA<CGFHHHHHHHHHHGFHHGDDEGCEGGGGEEFBFGGGGFGGGB0CFF:FFFFFBBFFFFBFEFA.BFFFEFFFBF/BF/FFEFFBFFF +@M01368:8:000000000-A3GHV:1:1108:16350:22621/1 +TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +AAAACFFFFFFFGGGFGGGGGGHHHHHHGBGGGFGGGHFGGGGGHHHHHGGHHHHFHGGGGGGHHGFHHHHHGEFHHFFHHGFCEFHHHGFF?GHHEE@ECFHHEGGGGBFFEGGGHFDHHGFG/BFEHHFCDGGGCDEGGGCGFFFHHHFEGHFHHHHGHHDGHHHGGHDED0CE;CFFFFBFF/;CBFFFGG0/FFF0B0:BB?.;BFF0FGFBFFFFFEFE;ADED.;BE.99FAFFFFFFF/;EFF/ +@1:A:M01368:8:000000000-A3GHV:1:1108:21791:23272/1_PolyA:1 +CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTT ++ +3ABBBFFFFF@FGGGGGGGCGGGHHHHHHHHGHHACGDAGFEEHGGHHGHHHHHHHHHHHGGGGGGHHHHGFHFGHGFDDGFFFGGFGHHFF?FEGHHGGGGGGHHHGFFDHHFGG?HEFGHHGGGDDCGFFC?ECCFGDEFGGGGGGFHHHHHHHEFHFCGHHGEGGGGD.:.A;CECAFFFFBFF/;EFFBFFBFB;;BFFFFBBAA./BFFEBB//BFFFFFFFDFFFEEFEFFFBFBFBBFFFFF. +@M01368:8:000000000-A3GHV:1:1108:23314:26089/1 +ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTG ++ +BBBBBFFF?FFAGFBFGGEGGGGHGHHFHHHGHHGFGGFHHHHHHHHFHHFHHGHHHGGGGGHHHHFFEHHHGGGGGGHFHGEEGF3FEFGHHHHHFFDEEEEHHHGEEGGGGGHFGGDADGFFFFFHHFGHHHFGBHGHHGBCEHDEDDGADCFFHHHGG/:GCCCGHFGHFFGG0CGFGF.FB/9;BFBB0FFBFG0A-..;C:E/.A9.FFFFFFFF9BFFFFFFBFBBB./:BFFF/F;AA?F//// +@M01368:8:000000000-A3GHV:1:1108:25084:18127/1 +TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGAGATAGACCTGTGATCCATCGTGATGTCT ++ +CCBBCFFFFFFFGGGGGGGGGGHHHHHGHHHHGFGGFHHHHGHHGGFHEHHHCGGGHBHHHHHHFHHGHHHHGGHHGGHGGGHHHEFHHHHEHGHGGGCFHFHHHHHHHHHHHGHEFGDEGGGHFHHCGGHHGGGGGFGHHGGEGDFHEGG/?GFHGHDGGGGGHHHHADFGGGGGEGCGGGEFGHHFEBFFGGG?GGGGEGGFEFAA?.0:A0;BFFFBFF/;9BFFFFEFF9FBFFB/F/.;FFFF/BB +@2:A:M01368:8:000000000-A3GHV:1:1108:7626:10048/1_PolyA:2 +ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG ++ +CCCCCFFFFFFFGGGGGGGGGGGHHHHEFEFHHGGGGGGHHHGEF?GGGGGGHHHHHHGGGEGEHHHHGGGGGGGGGGGGGGHHHHHHHHHHHHFFCGHGHHHHGG/BFGDGG2FFHFHGHH0FHFGFHHHHFFHHHHHHHGG<FHHHFGHHGGGGFHHHGGGGGGHGGHGGGHHHHHHFGGGGGGGFFFGGFGGBFGGGGGGGGGGGFGFFFCFFDFFFFFFFFF;./;AAAFFFFFFAFABBFFFB/ +@M01368:8:000000000-A3GHV:1:1109:11270:12255/1 +AATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAGGTAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTT ++ +CCCCCFFFFFFFGGGGGGGGGGGGHHHHFFFHHHGGGFGGFFHGEGCHHGGGGHHHHHHGGGGGGHHGHGGGGGGGGGCEGFCHHHHHGFHFHHHHGFHHGGFHGGGFD/EFGEFFDGFHHGEFCEHFFGHHHGHHHHHHHHHGCGHHHGGFGHHHHGHEHHCFFADGHHHD?CG.CGHBCBFBFFGGFFFGFFFFF.CFBFBCFFFFGFFFFFGGGFFFFFDFAFFFFFF-@DBFFFFBFD---.;BAA. +@M01368:8:000000000-A3GHV:1:1109:19224:11655/1 +AAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGCATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCTGTCTCTTATACACATCTCCGAGCC ++ +BBB@A4FFFBBBGGGGGGGGGGHHHHHHHHFHHHHGFGHGGGFFAGFHHHFHHHGHHHHHHHHHHHFHGGHGHHGCFFGHHHGHHHGHHFHGGH3BFFGHHGHGEFFH/EG@-CFGGCC@-;BD?FGGGGGG??FGDAGDFFFFEFF@BAAFEFFBFFFFF?FD9BBFFFF/FFFFBFFFFFFF?FFBFFFF/FBBFFBFFFE?F;@B//AFFFFFFFFF//;BFFEF.BFFFB/;F/99/FBFFBDCF;B +@M01368:8:000000000-A3GHV:1:1109:21978:9490/1 +GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCAGTT ++ +?A3AA?DFFFFFGGGGGGGFFGHFGCEGGFHHHHHHHHHGHCBGFEGGHHDEGAFGHGHFGDGBCGHHHHHFHHGHHHHHHHGHHHH1E?EGGGHHHHFGFHFHHHHHEDHHGHH@GGHGHHCGG?CFGFGGGGGFGB.BFGGFFGGFFFBDDBFFFFFF9-;DEFFDFBFFBB.EFF;BFBFFFFFFFFFFFFFFFFFFFFFFFFF/B9/FFFFFFFAFFFF--99AFFFFFFFA;;;FF..9//;/;;/ +@1:A:M01368:8:000000000-A3GHV:1:1109:22910:4079/1_PolyA:1 +TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTCCACTTTAGCT ++ +CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHGGGGGHHHHGGEHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGFHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGGHHHGGHGGGHHGHGHHHHHHHGFHHHHHHHHHHHHHHHHHHGGHHHHHHHHHHGHHHHHHGGGGGGGGGGGGGGGGGGGGFFGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFCCDFFFFFFF0BFFFFFFF +@M01368:8:000000000-A3GHV:1:1109:24217:19077/1 +AAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGCCTTTGATTCCTGCCTCATCCTATTACTGTC ++ +>AAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9////99/ +@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 +CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT ++ +AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGEC<CDFHHHHHE.FD11FFAGBGD.DGHEA<.<<0<EHAHCFHHF./;CC0GFH0::0CFGG0FBFD;9/90BFF0CBBFGB09;B:0;CFF0.-;@AFEEF--;AFFBF/99D-;.//.../9;B-. +@M01368:8:000000000-A3GHV:1:1109:27103:7153/1 +GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGCTGTCTC ++ +33>A?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.<GGEHGF0CHHHF/CAGC000CCGFHFGFGFFG?:CB990;F09;FB0BBGGGGGG?;A9EBDEFA///;FF/:F?BFBBFFBFFFF///;/9B;/// +@M01368:8:000000000-A3GHV:1:1109:8763:7859/1 +TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGCTTTATGC ++ +BABAADFFFFFFGGGGGGGGFBGFGHHFBHHHHGGHHGGEHGDHHHFGHEHHHHGHHGGGGAGHHFBHHGHFHHHHHHHHGGGCEFGFFFFEHHH?FFG1FFHHFEGGGHGFEEHHHHHGGGFGGGHHHHGGGGG/C/FG-<A<GFHHFFCGHHFHGE0<<CGHHHGCHG.CCCCCE00BBFGFFG.BB0FFBFBCF0;CBFFFEEGBBBFFBF/FFF9BF/9BAFFD9AFEDAA.A/BFFFFFFFFFEB/ +@M01368:8:000000000-A3GHV:1:1110:12808:18367/1 +AATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCCTG ++ +BBBBBFFFFBBFGGGGGGGGFGFGHHGGGGGHHHHHHHHGFHHGEGGGHHGFGHEEGGHHHHGHHGFFHHGGGGGHHHHGHGHGHHHFGGFHHGGGGGHHFFFGGHHHE?@EGGHFHEGGGHHGGGGHGEFHHGD?/>CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9 +@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 +TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG ++ +AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;/// +@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 +CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC ++ +BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA; +@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 +CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC ++ +ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9 +@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 +AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC ++ +BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD +@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 +ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG ++ +AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A<GFHFHHHGHBBGFEGGGGHFFFEF/EEGGHGGFEHFHHBA.<ADFFHFHB/CCGHFEFHHGHGEFFGGGGGFFFGGFGFFBFFGGBFGG??DGG?@9-ADFFFFB/@BD;./BFE?BFE/>99FE//BBFFF.9/FF?DA=@9./9 +@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 +ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC ++ +3ABB?FAB?DAAGGGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B; +@1:A:M01368:8:000000000-A3GHV:1:1111:13195:14562/1_PolyA:1 +ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG ++ +ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BF +@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 +GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT ++ +>>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9 +@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 +GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG ++ +3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/ +@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 +ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG ++ +CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>- +@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 +TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG ++ +AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A- +@1:A:M01368:8:000000000-A3GHV:1:1111:9655:13916/1_PolyA:1 +GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG ++ +1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG<GDHBFDGFFCGH//B//?0GFFGFGGHHHFHFB.=1FDFB?FGH.C/.-:::CH0;CAB@-.;0CGFFFGG--9.A@GB>--99@=---AAABA-BF/B?A@F-9BBFF//BB/9 +@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT ++ +AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A. +@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 +CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC ++ +AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//.. +@1:A:M01368:8:000000000-A3GHV:1:1112:9832:6701/1_PolyA:1 +CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG ++ +AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C<EGCECEFCFFHBGHE:GHHGFF0FFFB0CFGGGGAGFFF0;;FFGGGFFGFFCDD.DEFBEDD?9FFFFFFFFFFFFFFF/B./FB///;BF +@M01368:8:000000000-A3GHV:1:1113:13532:8618/1 +GGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGCTACAGTTCACTTTAGCTACCTCCAAGTGTTATG ++ +AA?AAFFFFFFFGGGGGFBFGGGGGGH5GHFEDHHGGGGCGFBGH?AEEHFFEEGHGHHHHGGG?0EHGHHGGGGGC?GEE/EE?GHHHGBHFHHHHHHGHHGHHHGGGCG/A@<FGGHHHHHHHFF/GFDGHHHHHHGFFFGHGGHABGHFHHHHHFHEGDGHGDDEEHHBEDDEGFGHGBFFBFFEGGGEFGFGFB000;F0F;FGGEGGEFFF;-.9//B///B9FFFFF/BFFB/:.BB//;FBFFF +@M01368:8:000000000-A3GHV:1:1113:21679:18011/1 +GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGAGGGTGTGCACGCGATAGCATTGCGAGACGCTTGAGCCGGAGCACCCTATGTCGCAGTATATGTCTTTGATTCCTGCCTCTTCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACTGTCTCTTATACACATATCCACGCCCACGAGACTAAGGCGAAGTTCATT ++ +AA>1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0<FFDGGCC?FF221<BDD11@<11@DDCGEF1<111F1<F1FBGHGHCF-CHHFA./</0CGHF<0CC/;C-:-;;09;FFBFBBFFBC0FFFGGFC0009C00090/-:--9--;-;AFFE;/99-9--/;/// +@M01368:8:000000000-A3GHV:1:1113:25528:14016/1 +CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGG ++ +>AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF +@1:A:M01368:8:000000000-A3GHV:1:1113:5741:16959/1_PolyA:1 +TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0<C;CGGGGEFF/.;0;FFFBF/0;0CFGFFB..9B/;0CBFFBBFFFFBAC?DED9;B9AD;.FFFB/B/;FBA/B/ +@1:A:M01368:8:000000000-A3GHV:1:1114:10130:11959/1_PolyA:1 +CGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTCTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAACAAACACACATCCAG ++ +>A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9;BF9FF9FFFFFFFFFFFFFF?BB?;9EE;-;DDB9//;B-B@;ABFFEFFFF/-.9A.;//9/BF./B/..9.9:...///// +@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 +CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTT ++ +AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE. +@1:A:M01368:8:000000000-A3GHV:1:1114:15066:16302/1_PolyA:1 +TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATTGCTTGTAGGAC ++ +BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<<AHHHGGGGGGGFHH0GHFCCCADGGG?.9/A-???DGGFFF.9F9/EE-;;BBBFFBFFFFFFFFFEFFFFBFFBBFFFFF/BFFBFFFFF-DBFFF;/BFF//BB//9/BEA---9:BFFFFFF/F/.;.:@9.BBFF/;BFF/;/////9///// +@M01368:8:000000000-A3GHV:1:1114:16639:15258/1 +CCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGCGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTCCAATATTACAGGCGAACATACTTACTAAATTGTGT ++ +11>>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/; +@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 +TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC ++ +CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/trimmed_polyA_reads_pair2.fastqsanger Fri Oct 08 09:57:52 2021 +0000 @@ -0,0 +1,396 @@ +@M01368:8:000000000-A3GHV:1:1101:6911:8255/1 +ATCTGGTTCCTACTTCAGGGCCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT ++ +BCCCCFFFFFFFGGGGGGGGGGGHHHHGHGHHHHHHHHHGGGGGGHHHHGHHHHHHHHHHGHHHHHHGGHGGHHHGHHHHFHHGHHHHHHHHHGHEHEFFGHHEGGCEFGGFHHHBGHHGHHHHGHFHHHGHGHGHGGCDFDDACGGGGGGGAAFFFFFFFFFBAFFFFFB;FFFFFFADDFFFFFFFFFFEFFFFFFFFFFBFFFFFFFFFFFFFFEFFFFFFFFBFEFFFFEFE;DFFFDFBFF/9BFB +@M01368:8:000000000-A3GHV:1:1101:14518:9998/1 +GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGAT ++ +AAAAAFFFFFFFGGGGGGGGGGHGGHHHHGHHHHHHHGCGHHHHHHHHHHHHHHHGGGGGHHHHHHHHHGHHGFHFE5BGEEHFGGGHHHHHHHHFBHHGGGGFHGHHFGHHHHGHHHHHHGEGGGGFHFHGEGHHGGCDGDGHGGGDGGHGGCGGGHGHHH/ACDG?.1FGCDCCGCA.CC@CDCHFHGFFGGGEBFGAB//CEFBFGG.:;D;;A0AFFFFFB..:@ABFF//;BFFFFFBF/9D:A// +@M01368:8:000000000-A3GHV:1:1101:18422:19051/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +CCCCCFDDDDDFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGHHHHHHHHHGHHHHHHHHHHHHGGHGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHGCGGGHHHHHHHHHHHHHHHHHHHHHHGFDHGFHCFGGGGFGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF;FFFFFFFFFFFFFFFFFFFFFFFFFFFFEFBFFFFFFFFFF:FFF. +@M01368:8:000000000-A3GHV:1:1101:25545:21098/1 +ATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATAAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGGCTTATTTAAGGGGAACGGGTGGGG ++ +33AA?DFD5BDFGGGFEBDGEGHEGHGEGHCEGGHHCHGHHFFHHGFGAGE53FF2FAFFGDE5FFFE5GFBFGAEE1GHHHGHHHEHE3FGHF@GEGEGGHHGG3FAGFFDE?EEE3GFEGFGFGGCG?GHHHFHGGGC@DHFFHD/A<C@EGFDCGGGHFHHHEGFGHBFHG0:CEHFCHGGED.;0CEF.F99B0CFFEEFGGG0FBFBBF0F/FFBDE?/9//9B.FFBFFFFFFBF..A..;@B-- +@M01368:8:000000000-A3GHV:1:1101:5446:12248/1 +AATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTAT ++ +CCCCDFFFFCCFGGGGGGGGFGHHHHHGGGGHHHHHHHHHHHHHHHHGBGHGGHGGHHHHHHHHHHGHGHGGGGGHHHHHHHHGHHHHHHHHHGGGGGHHHHFFGHHHGGGGGGHHHGFGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGGHHHHHHHHHHHHHFHGGGHHHHGGGGGG:FE;EGEGGGGG/;?FGGGGGGGFFFFGGFFFFFFFFFBFFFFFFFFFFBFFFFFFEFFFFFEFFF +@M01368:8:000000000-A3GHV:1:1101:5861:6452/1 +ATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTT ++ +ABCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHGHHHHGHHHHHHHHHHHGGGGHHHHHHHHFHHHHHHGGHGHGGHGGHHHHHHHGGHFHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHHEGGHHGGGGGGHHHGGGGHGGGGGHHHHHHGGGDCGHHHHGGGGGGGHEFGGGGHGHHHGHGGGFGGGGGGGEGGGGGGG?E0CEFGGGGGFEE9EEFFFFFBFFFFFFFBFFBD.AFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1102:10403:6021/1 +CGCTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTG ++ +>A@AAAAD2ADDFFGGGGGFGGHA?EAEFBEAGHFABFGG5FDF5DB1EEGAFDFB53FF5FH@G5FFEHGHEFHFFHBE333GF43GCGGGGE@0?BFGGB0B?FHGFDGGHHHBFFDEGGHGFFFDFE@<1>@FFFGHHHHFHEFGDABFFGG/@DCE<CG1<GF0/DD000=<DHGBDFDCECE/:AC?-;-;9B/ABBB-AD9BFB99AB?BDFBAD-.9..@;=;;..9..9/9;BEF;A:9/BFF +@1:A:M01368:8:000000000-A3GHV:1:1102:10677:23253/1_PolyA:1 +CCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCTGTCTCTTATACAC ++ +ABBBBFFFFFFFGGGGGGGCGGGHHHHHGHHHHHHHHHGHHHHHHHHHHHGHHHHHGGGGGHGHHHHHHHHHHHHHBGFHHHHGHGHGGHGGGCGGGHHHGGGGGGGHHHGHGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFDFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFEDBDFFFFFFFEFFFE0F0FBFFFF0FFFFFFFFFFFFFFFFF +@M01368:8:000000000-A3GHV:1:1102:13809:1733/1 +ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGAGGGCTATTTAGGTTTTATGCTGTCTCTTATACACATCTCCG ++ +BCCBCFFFFFBFGGGGGGGGGGHHHHHHHHHHHGHHGGGGGH3EHHHGHGHHHHHHHHHGGCEGGGHHHGGGFHGGGGGGHHGEAEFHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGCFFGHHFGHHHGHHHHHHCHGFHHHHGGHGGG00EHGHHHGFHHGEHGHFFFHHGGFHHHGGGGGGGGGB;;CFFGGFGFFBFFDFED;E.:A?DFFFFFFFF:FFFFF0BFFBBBFFFFFBFF0BFBF9F. +@M01368:8:000000000-A3GHV:1:1102:17584:10050/1 +ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT ++ +CCCCCFFFFFFFGGGGGGGGGGGHHHHHGGHHHGGGGGGHHHGFGDHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGEGGHHHHHHHGHGHHGHHHHGHHHHGGGGFDGHFHGEHHHHHHGFFHHHHHHHHHHHHHHHHHGGHHHHHHHHHHFHHHHHHGGGGGHHHHGGGHHGHHHGGGGGGGGGGGFGFFGGGGGGGCFGGGFGGGGGGGFFFFFFFFFFFFFFFFFFF;.BF9ADF/9;A;DFFF0 +@M01368:8:000000000-A3GHV:1:1102:18842:24844/1 +CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCT ++ +BBBCBFF@CCBBGGGGGGGGGGHHGHGGHHHGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHFHHHHGGGGGHHHHHHHHHHHHHHHHHHHHGGGHGGHHHHHHGHBFGHHHHHHHHBGHHHHHHGHGGGHFGCGGFHHFHFFHHBHHHHFFHFHHHHGGDHGGBC?;@DFBFFFFFFFFFBFFFFFFFFFFFFFF.B?FB.@;;DFFFFFFFFFFFEE-A./BBBFBFBFFF//BFB/BFF/ +@M01368:8:000000000-A3GHV:1:1102:21788:11027/1 +GCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGTGTTAATTCTG ++ +ABCCBCCBBFFFGGGGGGGGGGGHGHHHHHHGGHGGHHHHHHHHGHHHHHHHHHHHHHHHHHHGHHHHHFGGGGHHHHHHHHHHHHHHHHHHHHHHGHGHGGFCGGCGGGHHHGGGGGGGGGGGGGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFDFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEF9EFFFFFFFFFFFBFF0FFBBBBFBFBFFFF0 +@M01368:8:000000000-A3GHV:1:1102:5728:8777/1 +ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG ++ +BBBBBFFFFFAFGG5GGGGGGGHHHGHHHHHHHHHHGGGGGHHHHHHGHHHHHHFGHHHGGGGGFHGHGGGHHHGGGCEGHHGG?F1BGGGGGHHHHHGGGGEGGHHHHGGGGGCGHGGGGCGHHHHHHHHHHHHHHHHGGHHHHGGCEEDFDEGGHHHHHHHCEHHFHHHHGHGFHHHFFGGGFFFGGFGBGFFFFFFFFGFDGGD9EFA.DAFA/;FEFF9FB?/BBF/FFFFBBFFFFF/FFEFFFBB +@M01368:8:000000000-A3GHV:1:1102:5983:21093/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +AAABCFCABBCFGGGGGGGGGGHHHHHHGFHHHHHHHHGGHHHGHGHHHGGGGFHHHHHHHHGHHHHHHHHHHHHHGGHGGHHHHHHFHGGHHHHFHGHHEHHHHHHHGHHHHHD@FGGHHHHHFFFHHGGHGH?DHHHHHGHGHEGG/@?ADGFGGFFFFAFFFFFFFFFFFFFFFFFFFFFFFAAFFFFFFDFFFFFFFF;D9BFFFFFFFFFFFFFFFFFEFFFBBFFF0BFFFBBFF0EFFFFFFFA +@M01368:8:000000000-A3GHV:1:1102:7765:18353/1 +CTCCGGGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTC ++ +CCDCCCCCCBCFGGGGGGGGGGGGGGGHHHHHHDHHHHHHHHHHHGGGGGHHGHGHHHGHHHHHHFHHHHGHHHHHHGHGHHHGHHHHHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHGFFHHHHHGHHHHHHHHHHHGHHHHHGCFGGHGHFHHGHGHGHHHHGGFHHHHGGGHGBFGCC=BFFFFFFFF-@DFFFFFFFFFFFFFFFFFFFFFFAFFAEF.::FBFBFFE-A9:BFFFF0 +@M01368:8:000000000-A3GHV:1:1103:10405:17879/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +CBCCCFCCCCCFGGGGGGGGGGHHHHHHHHHHHHGHHHGHHHHHHHHHHHGGGGGGHHHHHHHHHHHHHHHHHHHHGGHGGHHHGHHGHHGHHHHHHHHHCGGFHHHHGHHHHHGCFGGHHGHHHHHHGFHHHGHHHHHHHGHGHGFF/CC?ADAFGFFFFFFFFFFFFFFDFFFFFFFFAFFFFFFFFFFFEDFFFFFFFFFFAFFFFFFFFFFFFFFFFFFFFFFBBFBFBFFFFFFFFFFFFFFFFF? +@M01368:8:000000000-A3GHV:1:1103:13493:8205/1 +GTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT ++ +CCCCCFFFFFFFGGGGGGGGGGGGGGGHFHHHHHHHHHHHHHHHHGHHHHHHHHGHHHIHHHHHHHGGGGFHHHHHHHHHHHHHHHHHHHGHGGHGGHHHHHHHHHGHHHHHHHHHHHHHHHHHGHHHH/FGGGGHHHHHHHGGEHHHHHGHHHHHHGHGHEFEFGGFFFFFFFFFFFFFFFFFFFFFAFDFFFFFAFFFF;;@FFEFFFFFFFFFFE=?AFFFFFFFFFFFFFFFFFFBFBBBFFFFEFF +@M01368:8:000000000-A3GHV:1:1103:14060:21273/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTACTGTCTCTTATACAC ++ +AAABAFCCCCCFGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHGHHHHGGGGGFHHHHHHHHHHHHHHHGHHHHGGHGGHHHHHHHHHHHHHHHHHH?GGHHHHHHGHHHHHFEGGGHHHHHHHHHHHHHHHGFHHHFHGHGHGFGCGG@@BCFGFFFFFFF?FFFFFFFFAFFFAD?@FFEEFFFFFFFFFFFFFFFFFFD9BFF/B/FFF/:/9FB9/BFA//;BFB:FFFBB/BFFFFFBFFFFB/ +@M01368:8:000000000-A3GHV:1:1103:14528:12993/1 +GCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGAT ++ +BBBBBFFFBDFFGFBCG2EGFFGHHCF5AFHHHFHFHHHGG?CEHBHHDGFHHGHHGHBGHABFFBGHGDGGGFHGHHGGHHHGGG@E1GEHHHGHHFHH3FEDHHHHGFGHGFHHGHFBGA?FGHHHHHGHHFFGHFDFGGHHH2??<ECFHHGHHHHHHHHHGHH<1DFGHGC<.FCB/<=@DCFFFFFEDA=-AAB9B/9B9B-@?.-;A-AFF.=-=BFEFEDEFFFBFEA-A.B//9F9F//;BBB +@M01368:8:000000000-A3GHV:1:1103:17657:20856/1 +AAAGCGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTAT ++ +ABB?AABBBBFFGGGGGGGGGGHHHHHHHHGHHHHHHHHHHHHHHHHHGHHHHHHGHHHHGGGGGHHHHHHHGHHFHHHHHHHHHHGGGGGHHHHHHHH3BGGHHHHHHHEHHHHHHHGHHHHGEEGGGHHHHHHHHGHHFHGHGHGHGHHCHGGECCDFGB@AFFFFFFFFFFFFFFFFFDAFFFFFFFFFFFFFCAF;AFFFFEFFBBEFFF9FBBFFFFFFFFFFEFFFFFFFFFFFFF/FFFFBFF9 +@M01368:8:000000000-A3GHV:1:1103:23099:3794/1 +AAGACAGATACTGCGACATAGGGTGTTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCTCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGGATGCCGTCT ++ +BBBBBFFFFFFFGGEEGGFGGGGCFGEHCGGGGGGFHFFEDEHFGGDEHHHHHHGGFECGHHHHGFEFGCGHGGFGGGHBGFH3/BF3GGGHHEEEHHG22???BCFFFFFFHHFHFF@FGHH11GHHHHFGFHHHHGGHHHHEFFGFGHFG1DGFGGGGEDCCCAEGGFFHHFHHHHHHCFBBFGBFFF/0;B0B00;B0BBFGBB9BFGEFGGGFFDEEAB;BDDBFBFFFFF@;.9BF..;-./-A=F +@M01368:8:000000000-A3GHV:1:1103:25465:20010/1 +GACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATCGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAACAGAC ++ +ABBBBFFFFBAAGGGFEFGGGGHHFEFFHFGGGGGHHHGHFGGGGGHHHGGGGGGGGHGEEEGGHHHGFHEHFHDGHGHEHGF?FGG?GGGGHHGHF4F?2?DDCDHGHHHHHFG2@FGHHHGH?CFGGHFHFHHHHGDDGGHGGGGGHGFH.<AFAGHHHFHGHHFCGFHHHHHGHHHHBGHHGGDGAGF.CEGCFDGGGGGEFFF=B.FFFEFFFFFFADF?/9//BFF.B/;:>---;--..;.../9 +@M01368:8:000000000-A3GHV:1:1103:25519:6301/1 +AAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGCATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATTTCGT ++ +3A3AAFFFFBFFGGG2CF?4FGHGHHCHGGHHBHGFFAGGHHFFHHCGHFEGCECEGHFFFHGHHGFGHFGDAFDGFFEEFEGGBECC/<A@DFBBEGC--:;GHHHGBECG@:9-9.-9C/ABB?B@?.EDAFFFFBAFBFBFBFFFFF//;BBFFFF/;/9BBF/BBFBFFFFFFFFFEFF??BF/.AF./;B/9FFFFFFBFBFFFBFFFBFFFFF/BF/A---9.9A-B?DFFF/B/B?---.//;. +@M01368:8:000000000-A3GHV:1:1103:6074:6549/1 +TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG ++ +CCCBCFFFFFCCGGGGGGGGGG5GFHHHHHGHHHGGGGGHHHGHHHHHHHHHHHHGHHHHHHHHHGGHHHHGHHHHHHGGGGHHHHHHHHHHHHHHGHHHHHHHGGHGGHHHHHHHFGHHGHGHHHHHHHHHHHHHGHHHHHGGGGCHHHHEHGHHHEGHHHFGGHHHHGHGHGHF@CCBDFAFFFFFF-CDFFFFFFFFFFFFFAFFFFFEFF;BFF?FEDFFFFFFFF-A./FFFFFFFFFFFF/BFF/ +@M01368:8:000000000-A3GHV:1:1104:11572:8264/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +BABCBFBACCCFGGGGGGGGGGGHHGHHGHHHHGFGCHGHHGFFHEHHHHGGGGFGHHHHHHHHHHHHGHHHHHHHGGGGGHHHFFHHGHHHHHHHHHHHEHHHHHHHGHHGHGGEGEGHHHFHHHHGHHHHHHGGHHHHHGHGEGEHCGG-BGDFGFFFFAFFDFFFFEFFCFFDDFFFFFFFEC>;>=EF?AEFFFF;9;=DAFFFFBBFFFBFFF//;BFB.:FBFFBB/BFFFF/BFFE?EFAFEF9 +@1:A:M01368:8:000000000-A3GHV:1:1104:11928:24197/1_PolyA:1 +ACGTAGGTGCGATAAATAATGGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGAT ++ +CDDCCFCFFFBCGGGGGGGGGGHHHGHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHGHHHHGHHGHHHHGGHGEEGGGGFHHFHHHGHGEHHHHHHHHHHHHHHHHHGGGGGGFGGGGGGGGFFGGGFGFGAEFEFGFEAFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF@D +@M01368:8:000000000-A3GHV:1:1104:12288:20635/1 +CACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGCCGCAGCATCTGTCTTTGATTCCTGCCT ++ +ABA?AFBAB2>AFGGGGFFGD5GGHGFFHGHEGGGGHHHHFFHH5FHGHHHFFHEFHHHHHHGGHHHHHGFHGGHGGGGFHGGGFBHHHFGEGHHHHGHGGGGECEHHFHHGHHFFHFGGGHHHEGHDGHHGHGHFHHHECGCBCHHGFHHFHD1GFHEFHEF?GDCFFEFE0A@;@>BD/9BFBBBBFDFFFF99D=?>DDBFF-AFFEBB9BBEFEFAFFF//-@---;;FBBFBFB///;99999BA9 +@M01368:8:000000000-A3GHV:1:1104:13832:4398/1 +CACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGACAAAAAAAAACACACAACAACCAACACCCAGTAGACTAGTGCGTC ++ +AAABABCAFFFFGGGGGGGGGGHFHHHHHHHHHGFFHHGGHGGGGGHHHHHHHHHHFHHHHGHHHHHGGHGHGGHGGGGGGGHGFFGGGGDGGHHHHGGGGFGGGGGCFGGEGCDGGGGGGGGGGGGEGF?;BFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF/FFFFFFC.;BFEAFFFFF;@FFBFFFFEF?/FFFFE----:...:...9....9.9...:.9//9////;/9-.- +@M01368:8:000000000-A3GHV:1:1104:23670:4161/1 +ATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCC ++ +AAAABFFFFFFFGGGGGGGCGBBGGHHHHHHHHHGHHHHHHHHHHFGGHHHHHHGGFHHHHHHHGGGGGFHHHGHHHHHGHHHHGHHGGGHGGGGHHHHHGHHHHHHEGGGFHHHGFFDGEGHGHFHGHHGEGGGHHBGHGGHGHGGGGG/@FCDFDGFHGGGDFFHFGFFGGGGGHHHHGGGGGGGEGCDG?GGBFFFEE00BFFGABBBAFGGGEGEA.9FFF/./;BFBF./AAF/BFFBBF/F/BFB +@M01368:8:000000000-A3GHV:1:1104:24927:22253/1 +AGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTAT ++ +AAA?AFFF3DFFFGGBGFF3EAEEEHFHHFHHHGHHHHDGHHGAEFGHHHGFCHHGHHHBGHHHAEGGHGGFHFBFHHHHHHHHHHFHH1EEHEEHH1FFDEEFCGHBGHHFHEHHHHHH1FCGHHHHEEFGEEGFFCHHH11>GHHF/FG2DGGCGAGGHGGGG@@@BEFFF?@@@FFEFFFFFF?@@FB@BB@FFFE=;==---A9EFBBB/;E;A9B/B/FFFEF/B///;/;9/A/;/9BFFFBFBB +@M01368:8:000000000-A3GHV:1:1104:4705:7727/1 +CTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGG ++ +BBBBBFFBFFFFGGGGGGGGGGHHHGHHHGHHHHH6GHHHHFFGGGHHHHHHHHHHHHHHHGGFGGGEGGGHHHHHHHHHHGHE3FGGGGHHHHHFHHHHHHHHHHHHGGGGGHHFHFCGFHHGGGGGGGHHGGGGHHGGGGGGHHHHGGGADCHHHHGDGGGGGGGGC@DGFHEHHHHHHGHHHHGFGDEGFGFFGGGGCFGBEGFGGGFG;C.BFFFFFFFBFBFFFF/F?.AFFF/BFFEFFFFFEFF +@1:A:M01368:8:000000000-A3GHV:1:1104:7756:4776/1_PolyA:1 +GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG ++ +CCCCCCFFFFFFGGGGGGGEEFHHHHGHGHHHHHHGGHHHGGGGGGHHGHHHHHGHHGHHHGGFHHHHHHHHGHHHFHHGHHGFGGGGHHHHHHHHGHGHFFHFHHHHHHGGHGFGFHHHGHG?FGHHHHHHHHHHHHHHGHGHHGHGGCDGFFHHHHGHHHHHGHHHGFHGHFHGHGHGH/@CABFFFFFFFACAFF?BBFFBFD-@-.D>FFAF.F.:9---;AADFFFFFBEFFF:9FFFFBBFFFF +@M01368:8:000000000-A3GHV:1:1105:11310:21761/1 +ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTTCACTTT ++ +CCCCBFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHGHHHHHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHGHHHGHHHGHGHHGHHHHGGHGGG=FFGEHHHHHGHFGGHHGHHHHHFHHHHHHHGGHHHHHHHGHEHHGHHHHHGGGGF9AGE?DEGFFGGGGGGGGGGGGGGFFFF/9FFBFFFFFFFEFFFBFFFFFFFFFFFFEFFF0 +@M01368:8:000000000-A3GHV:1:1105:11578:14676/1 +ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTC ++ +CDDDDFFFBFFCGGGGGGGGGGHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHFGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGHHHHGGGGGGHGGHGHHHHHHH;CHHHHHHHHHHHHHHHGHGGGGFGGGFGGGGGGGGGGGGFGGGEGGFFFFFFFFFFFFFFFFFFFFFFFFF/FFFFFFFFFFFFFFFF0 +@M01368:8:000000000-A3GHV:1:1105:13575:19255/1 +TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGTTCCCGTGAGTGGTTAATAGGGTGATAGGCCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +AA1A1DFFFFFBGG3A111FFAEGDDBGFDHF00F01BBA00BFGEHFBFFA1FABB0FAE?CFHF1BGFHFB0011F121E//>>1F10>B?EFHGCC/?/BFG/EEGFFEE//BCFDE0?E@@B/?11</?//<@?/F<<<A-<FBGH0.00=G0<<C//./0;;GC....<C.=.00;;BB./:.900////.C000;0090;..//000;C/;;/B/9BA9A9-AEEFF?B---;B99//9/;A//9 +@M01368:8:000000000-A3GHV:1:1105:15502:11453/1 +GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCTGTCTCTTATACACATCTC ++ +A3AAAFFFFFCFGGGGGGGGGGHHGHHHGHHHHHHHHHFGHHHHHGGGGHGHHHHHGFHHHHGHGGGHHGGHGGHHHHHHHHHHHHHHGGGGGFHGHHHGHHHHHGHHHHGGGGGGHGEGFECHEHGGGGGGHHHGADFHHGGGGHHHHH<ECGGGGHHHHGGGGGGGHGGGGGGHHHGHGHHGEHBFFFGFGEGGGGGEDEGF0C;;BFFFGGGBEFFE/FFFFFFFFFFFFFFEFFFFFFFFBFFFBFF +@M01368:8:000000000-A3GHV:1:1105:16787:9771/1 +GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT ++ +AABCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIHHHHHDHHHHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHGGGHHHGGGGGGHHHGGGGHHGGGGGHHHHHGGGGGGHHGHGGGGGGGEEGEGFGHHHGGGGFFGGGGGGGGGGGGGGGEG?G;:EFFFFFFFFF:AEFFFFFFFFBFFFFFFFFFEFF/ +@2:A:M01368:8:000000000-A3GHV:1:1105:17564:15723/1_PolyA:2 +TATGCTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG ++ +EDCCDFFCEEDCGGGGGGGGGGHHHHHGFGHGGHGGHHHHHHHHGHHEHHGGGGGHHHHHHHHHGHHHGHHHHGGGGGHHHHFFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHFEHHHHHHHHHHGHHGHGGHGGGCGGGGHHH1FGGGFGGFGGGGGGGGGGEFGGGGGGGGGGGGGGGGGFFFFFFFFFFAEFFFFFFFFFFBBFBBFFFFFE1:BFF +@M01368:8:000000000-A3GHV:1:1105:18108:12091/1 +TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACAC ++ +CCCCCFBFFFFFGGGGGGGGGGHHHHHHHHHHHHHGGGGGGFHHHBFGHHHGGGGGGHHGGGGGHHGFGGHHHHHHGGGGGGHGHFGGGGGGDGEGEFGCHHHHHHHHHHGHHHHGEDHHHGGGCDGGGGFHFHGHHHGHHGHFGHHHFHHGFHHHFHHGGHFHFHHGB0CGHGFHCBGGGGEDEE9ADFEFFFGFBFGFFGGFFFFFGGFGFFFFFFFF/A?FEFFFFFEF-A.B//;BFFEF//BB/// +@1:A:M01368:8:000000000-A3GHV:1:1105:18331:16683/1_PolyA:1 +TCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATT ++ +BABA?DFFFFFFGGGGGGGGGGHHHHHHGHGHHGGGHHGHGGGGFFHHGHHHGHHHHHHH3FGHHGGGHGGHHHHFHHHFHGHHHGHHHHHHHHHHHHGHHHHGGCGGGHHGHHHHHHHHHHHHHFHHGHHGHGHEFGGGGABFFFGBEGGAFFFFFFFFFFFFFDDFF?DFFFFFFFFAEFFFFFFFFFFFFB;9FFFFFEFFFFFFFFFFFF?FFFFFFF/BFFFFFFFB.DABFFFFFFB.//:FB/ +@M01368:8:000000000-A3GHV:1:1105:20577:18421/1 +GGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCC ++ +ABB@3>AAFFFFFEAGGGFGGAA6FHDGGCGFHFFHFHFHHHHHGDDDFFFHGFFHGBEGEGHHHFFFHGEEAEGHHHHHFHHHFHGHFEDGGEGBEAGEFHHFFHHHHHHGGHHHGHHGBGHHFHHFGHHHHG?CG?AFHH1FHHHGHFHHHF?DGHFFHCGAGEFHGCBBBB?BFFF?B-B.9/BFFFFFB=-AD?..@FFFF@@-@@?AFD.FFFBBEEBD9/:FFFFFBB/:FF9BF/BF.BBFF/: +@M01368:8:000000000-A3GHV:1:1105:21099:2895/1 +TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTT ++ +CBCBBFFFFFFFGGGGGGGGGGGGGFHHHHFHGHHHHHHFHHHHFGFHHHHHHGHHHHHHGHHEHGGGGHHHHHHHHHHHHHHHHHHHHHHGGHGGHHHHHHHHFHHHHGHGHHHGGHHHHHGGHFFGHGCGGGHGHHGHHHFGHHFHH/FGGGHFGFEGGCFCCD;B@DFFFFFFFFFFFFFFFFFFFFFFFFF;F.BEFADFFFF.AEFFFFFFE;D.;:B00099:09BFBFFBFF.0:000BFFBF0 +@M01368:8:000000000-A3GHV:1:1105:26469:19245/1 +TTATGATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCAAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATG ++ +BAAAAFFFBFFFGGGGGGFFGGHHHHHHHHHHHHGGHHGHHHGGHHHHHHHHHHHDEGGHGHHHHHGEDHFHGFHGGHHGGHGGEHHHGHHGHHGHHHGGEGGGHGHGHHHHHHGHHHHGHGGGGGHFHGGGGHHGGGGCDGHHHGD<EHHGGGGGHHGHH?EDCCDHHHHDGFFGGGHGGFCGGHHHHFG.CFGGGGGGGGGGGBFGDEG.AFFG;AFFFFEB?/A9BBBFFFFFFF/9FFFFBDEFFFF +@M01368:8:000000000-A3GHV:1:1105:7169:17145/1 +AACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAAAGTGT ++ +CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHBHHHHHHHGGHGGHHHHHHFHHHHHHHHHHHHHHHHHHHHGHHHHHGGGGCHHHHHHHHGHHHHHHHFHHHHHGHGHGHHGGGGGFGGHHGGGGGGGGGGGGEGGFGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFDFFFFFFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFF-9@DDBFFFFFFFFBBFFF0 +@M01368:8:000000000-A3GHV:1:1106:15481:16176/1 +CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTT ++ +ABBBBFFFFFFFGGGGGGGGGGHHGHGHHHGHHHFGHGFGHFEHGGHGGHHHGHHHHFGHGGFGFFHHFHHHHEGHFHHHHHFGGGGGHGGGHGFHGHGGGGGGHHHGGGGHHGGGGHHHGGHGGGGGGHHHHGGGGGCFHGGGCDGHGHHHFHEHHHHHHHHHEEFHHGG:C:?0<EFGBFGGGGGFE.BFGEFFFGFFGGGGGGFDEF/FFGFFFFFFF;BBFA;.:EFFE:AAFEF.BFFFFF;9FF. +@M01368:8:000000000-A3GHV:1:1106:17389:18671/1 +CCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGGCTTTGATTC ++ +3>>A@FFFFFFFGGGFEECFGGFFB5GBHH5DFH5GBEEAEFHFHHF3F3GHFHHHEF3GAEHBGFHFFEFFHHHHFFB31FGGEGFHGEHHHHHHHHHHHHHHHHHEEEEGHHHFFF3GF3FGF44BBGEHHHHGG2B/BGGHE</ECDFHHBF1DGBFBFGFH<1GHGDGGFDFE0/<--;-9./BBED;BFBD/BF9BFFFDBFDADD;FFF?->;B=.9.;.BBFFF..;A;/B/;B//;;//BBF/ +@2:A:M01368:8:000000000-A3GHV:1:1106:22927:3863/1_PolyA:2 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACT ++ +A>ABAFA?D@AFGGGGFGGGFGFHHHFFH4BCBGHHHHGFFF3G5BF3322EEAFHHHHHHAB5GHFHEHFHHH3BEEHFGF5@13FDF3GDGFHHFGHHHGHHHGFGGHHFCG//?/EGHHHG3F4BFFGHHHEDGFHHB/B/?FBF<@-9?BDEFFF?B--?DFFFB9FA>B;A.BFDB?AB.--@;-.FFAEFFFF9AE;DDFFFFB/9BFFFFFFFB:BF/BB/BFF//FFF-=--.99=--.9/ +@1:A:M01368:8:000000000-A3GHV:1:1106:23625:4236/1_PolyA:1 +TAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATT ++ +@?AAAFFBFBFFFGGFGFGGGEFCGGHHHHFGHGHFHGHHHHGGFGGFGHHGHGHHHHGHGFFHHGGGGGHHHHHHHHHBFHHEFHHHHHHGF?FAGHGFHHHGDGFHHGGGHGHFHHHGHHHGHHFHHGFFGFHHHGHHHHFFHHHHH/FGHHHHGHGHG<C???B=@ABBFEF@=-AFFFFFFBFAFFDFFFFA?AFFCFFABEFFFFFFFFF?FFFDFFBFF;FFEFFEFFFFFFFAF///9FF/BB +@1:A:M01368:8:000000000-A3GHV:1:1106:26031:14826/1_PolyA:1 +GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGTCTTCTGCTTG ++ +ABBAAFFF@FFFGGGGGGGGGGHHHHHHGHHHHHGGGGGHHHHHHHHGHGHHHHHHHHHHHGHGHFHFGE?CGDGGHHGGGGGGGHHHHGHGGGGGGFGGGHGHGGGGGGGGGGGGGGGGGGDGAFFGFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFB/9FFBFFFFFFFFFFFFFD@D=BEFFF;FDDB////B=CF-.;BAFFFFFF;;BFE/BFFFFB +@1:A:M01368:8:000000000-A3GHV:1:1106:27607:15990/1_PolyA:1 +GACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGACTTCTGCTTGAAAAAAAAAAACTACAACCCACGGACGC ++ +BBBBBFFFFFFFGGGGGGGGGGGGHHHGGGGGGHHHGGGGGFCEGGHHHGHHGGGEGGHHHAFGGGGGGAFG//EGHHHHCGEHHHHEEHHFHGHFHHGGEF/FGGC?FDFH2DHHFFGGFGHHHHGGHHFGHHGHGHHGFHDHBGBBGHHFHHGGCCDC<=D<DGHHC;CGFCCFBECDG?FGGA-;EB9FBGFFBBF>DFFFE?FFF/--;;9/:BFFFBA///9----;B.//////;...---9-- +@M01368:8:000000000-A3GHV:1:1106:6455:17124/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACG ++ +BBBAAFABDBBFGGGGGGGGGGHHHGHHGHHHHHHHHHGHHGHHGHHHGHGGGEGHHHHHHHHHHHHHGHHHGHHHGGHGGHHHHHHHHHHHHHHHHHHGHHHHHHHHGHHHHHGGCFEHHHGFHHHHGHHFFH?GGHHHHGHGHGHHCFGGGGGGGFFFFFFFFFFFFFFFFFFFFADFA?.FEFAFFFEFFFFFFFFFFFFDABFBBFFFFFFFFEFFBFFFFFFFFFFF/FFBFFFFFFADE>BFFF9 +@1:A:M01368:8:000000000-A3GHV:1:1107:14451:26748/1_PolyA:1 +GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG ++ +BABBBFFDABBBGGGGGGGGGGHGHHHHGAGHGGGGGHHHHHHHHGHHHHHGGGGGGFFHHHHHHHHHFHHHHHGGGGCHFHH3FEHHGCGGGDGHHHGFGGHHGGGGHHHHHHG/DGGGHHHHGGGGGCCHGG?CGG1GHHGHHHHBGHHHFHHGHHHHGGADAD00ECEGHEFHHFFAEFFFFFGGFBFBFFFGFFG.;B/99;BCFGGGGEFFFFFFEDA./:ADEB?FBFFFF;FFEBFBFFB./9 +@1:A:M01368:8:000000000-A3GHV:1:1107:18714:24788/1_PolyA:1 +GTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTG ++ +BBBCCFFFDEDEGGGGGGGGGGHHHHHHGGHHGGHGGHHHHHHHHHHHHHHGGGGGHHHHHHHHHHHHHHHHHHGGGGGHHHHCFGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHHHHHGHHHHHHHHHHHGHHHHGGEGEGCCEGGHHHHHHG:CAFFGGGGGGBFEFGGGGHGAFFGGFGGGGGGGEFFFFFFFFFEF/EDDFFFFFFFFFBFFFFFBBFFBBF +@M01368:8:000000000-A3GHV:1:1107:21355:8333/1 +ATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATG ++ +ABCBBFFFFFAFGGGGGGGGGGHHHHHHHHFHHHHHGGGGGHHHHHHFHHHGHHHHHHHGGGGGHHHHFFGHGHGGGGGGGHGGEGGGHGGGGHHHHHHGGGGGGHHHHGGGGGGGHGGGGGGHGHHHFHHHHHHHHGHFFHHHHGGHGEGHHGHGFHHFHGHCEFHEGHGHHHGHHHGGGGGGCFFFFBFGGFGGGGFBG?FDGGGEGFFFFEFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFF.AA/9 +@M01368:8:000000000-A3GHV:1:1107:22864:15712/1 +AATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG ++ +BBBBBFBBFFFFGGGGCEGGGGHGHHDGFHHHHHHHHHHHHHCHHGHFGBGDFGE?GHHGHFBHHFHHHHHHHHHGHFGFGHGHHGGC@DCFGHHGGGGGGGHHHHHHGGGGGFGGGGFFGGGGFADGFEFFFFFFFFFFF9;FFFF/BFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFEAECDFFFFFFFFFBFFFFFFFFFFFFFFEFBFBFFF/FFFDF-;-9;E@>-9DFFF/FFBF;-.:;.9A// +@M01368:8:000000000-A3GHV:1:1107:24817:14233/1 +CTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACAT ++ +3AABABFFBFFFGCFGGGGGGGHHHFGCFGGGFFGHHCGHHHGHGHGHBFFHHHEDGGFGFGHHFFFHGGEGHFHHGHFHGFFHHHFGHHHFHACECEHHHBGFHGFHHHFHHF4GFHFHFHEHGGE/EG:A-CFGGFGADGGGGFGGGGDAAAADFA.-AFFECFDAAFFFFEFFFFBFF;D;BFFFB/;BFFFFFFFFFFFFFFBFFBF/;//;BFFFF?FFFFFFEDAFFFFFBF//:BFBACF--9/ +@M01368:8:000000000-A3GHV:1:1107:24830:14440/1 +AACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACC ++ +ABBBA@FFFFBBGGGGGGGGGGHGHHGHHGHHHGGHHHHHGHGHHGDHHHHHHAGGGGFHHGHHHHHHGHHHHHHHHHHHEGAFGFHFHHHHGEGHHHHHHHHHHHHHHHHHGGHHHGEFEGGHHGHHHHHGGHGFHH/?GHHHHGFGHGFHCGGBBDAFFFEFFFFFFFFFFFFFF=CDDADDFFFFEF=BFFFEFFFBFFFFEEBF./:FFFFFFFFFFFFFFFFFFFEFFFFFFFFFFF/BFBEFFC- +@M01368:8:000000000-A3GHV:1:1107:26478:23602/1 +GCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATCTGTCTCTTATACACCTCTC ++ +1AAAAF1B13BBBEGGGFGGGGGGHHDB1BGFHHHFGFGGG0F00BGBGFGGF1GBGB1BGCGAEE/FGFHGHFHHHHHBFF1B0FGGF//F//FGFGFFF/1@B1DFHHHGGEFEDHFDFAFF0B//</ECCC>1FDD1GFFHHDF?1BD>GF/GCAC0C@?@-;99BBB->@--;-B//99B--9-BF=A--A-:A----9AA9AFBFBBB?E@BBFFFFFFFBFFFFFBFFFBBFFF//B/////-;9 +@M01368:8:000000000-A3GHV:1:1108:11059:23181/1 +GTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGAT ++ +CCCCCFFFFFFFGGGGGGGGGGGHHHHHHHHHHHHHHHHGHHHHHHHHHHHHHGHHGGGGHIIIHHHHHHHGHHHHGHHHGGHGGHHHHHHHHHHHHHHGGGGGHHGHHHHHHHHHHHHHHHGGGGGHHHHHFGHHHGGGGGGHHHGGGGGHGGGGHHHHHHGGGGGGHGGHEFGGGGGHGGG-ECHHHGGGGGGGGGFGFFGGGGFGGGCF?EFGG0;BFFFFB?9AAFFFFFFBF;BBFFBFFFFE?F/ +@M01368:8:000000000-A3GHV:1:1108:12233:13498/1 +TAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCCTGTCTCTTATACAC ++ +BCCBAFFBCCCCGGGGGGGGGGHHHHGGGGGHHHHHHGHGHBGFHHHHHGGGGGHHGGBFGHHGGGGGGGHHHGGGGHHGGGFHHHHHHGGGGGGHGHHGGGGGGGGGGGGGGHHHHFHHHGHGHHHHHHGHGHHGGFGGGDFFGGHHHGHGH/<GHHFGBGFHFBGHHHGHHGEFHFHFGBF0BFGGGFGGGGGEFFF;A?EEG?CFFFFF0:BEFFFFFBFFFFFF0FFEFF?EFFFF0BFFFFBBB00 +@1:A:M01368:8:000000000-A3GHV:1:1108:14328:14751/1_PolyA:1 +ATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATAC ++ +BCCCBFFFFFFFGGGGGGGGGGHHHHHHHGHGHHHHHGGGGGFHHHFFDGHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHHGHGGGGGGGGGGGCFGHHHHHHHHHHHHHHHHHGHHHHGGGDGGFGGFGGHHHHHH>FHHHFHGHGHHGHHHGHHBA<CGFHHHHHHHHHHGFHHGDDEGCEGGGGEEFBFGGGGFGGGB0CFF:FFFFFBBFFFFBFEFA.BFFFEFFFBF/BF/FFEFFBFFF +@M01368:8:000000000-A3GHV:1:1108:16350:22621/1 +TTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +AAAACFFFFFFFGGGFGGGGGGHHHHHHGBGGGFGGGHFGGGGGHHHHHGGHHHHFHGGGGGGHHGFHHHHHGEFHHFFHHGFCEFHHHGFF?GHHEE@ECFHHEGGGGBFFEGGGHFDHHGFG/BFEHHFCDGGGCDEGGGCGFFFHHHFEGHFHHHHGHHDGHHHGGHDED0CE;CFFFFBFF/;CBFFFGG0/FFF0B0:BB?.;BFF0FGFBFFFFFEFE;ADED.;BE.99FAFFFFFFF/;EFF/ +@1:A:M01368:8:000000000-A3GHV:1:1108:21791:23272/1_PolyA:1 +CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTT ++ +3ABBBFFFFF@FGGGGGGGCGGGHHHHHHHHGHHACGDAGFEEHGGHHGHHHHHHHHHHHGGGGGGHHHHGFHFGHGFDDGFFFGGFGHHFF?FEGHHGGGGGGHHHGFFDHHFGG?HEFGHHGGGDDCGFFC?ECCFGDEFGGGGGGFHHHHHHHEFHFCGHHGEGGGGD.:.A;CECAFFFFBFF/;EFFBFFBFB;;BFFFFBBAA./BFFEBB//BFFFFFFFDFFFEEFEFFFBFBFBBFFFFF. +@M01368:8:000000000-A3GHV:1:1108:23314:26089/1 +ATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTG ++ +BBBBBFFF?FFAGFBFGGEGGGGHGHHFHHHGHHGFGGFHHHHHHHHFHHFHHGHHHGGGGGHHHHFFEHHHGGGGGGHFHGEEGF3FEFGHHHHHFFDEEEEHHHGEEGGGGGHFGGDADGFFFFFHHFGHHHFGBHGHHGBCEHDEDDGADCFFHHHGG/:GCCCGHFGHFFGG0CGFGF.FB/9;BFBB0FFBFG0A-..;C:E/.A9.FFFFFFFF9BFFFFFFBFBBB./:BFFF/F;AA?F//// +@M01368:8:000000000-A3GHV:1:1108:25084:18127/1 +TTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGAGATAGACCTGTGATCCATCGTGATGTCT ++ +CCBBCFFFFFFFGGGGGGGGGGHHHHHGHHHHGFGGFHHHHGHHGGFHEHHHCGGGHBHHHHHHFHHGHHHHGGHHGGHGGGHHHEFHHHHEHGHGGGCFHFHHHHHHHHHHHGHEFGDEGGGHFHHCGGHHGGGGGFGHHGGEGDFHEGG/?GFHGHDGGGGGHHHHADFGGGGGEGCGGGEFGHHFEBFFGGG?GGGGEGGFEFAA?.0:A0;BFFFBFF/;9BFFFFEFF9FBFFB/F/.;FFFF/BB +@2:A:M01368:8:000000000-A3GHV:1:1108:7626:10048/1_PolyA:2 +ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG ++ +CCCCCFFFFFFFGGGGGGGGGGGHHHHEFEFHHGGGGGGHHHGEF?GGGGGGHHHHHHGGGEGEHHHHGGGGGGGGGGGGGGHHHHHHHHHHHHFFCGHGHHHHGG/BFGDGG2FFHFHGHH0FHFGFHHHHFFHHHHHHHGG<FHHHFGHHGGGGFHHHGGGGGGHGGHGGGHHHHHHFGGGGGGGFFFGGFGGBFGGGGGGGGGGGFGFFFCFFDFFFFFFFFF;./;AAAFFFFFFAFABBFFFB/ +@M01368:8:000000000-A3GHV:1:1109:11270:12255/1 +AATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAGGTAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTT ++ +CCCCCFFFFFFFGGGGGGGGGGGGHHHHFFFHHHGGGFGGFFHGEGCHHGGGGHHHHHHGGGGGGHHGHGGGGGGGGGCEGFCHHHHHGFHFHHHHGFHHGGFHGGGFD/EFGEFFDGFHHGEFCEHFFGHHHGHHHHHHHHHGCGHHHGGFGHHHHGHEHHCFFADGHHHD?CG.CGHBCBFBFFGGFFFGFFFFF.CFBFBCFFFFGFFFFFGGGFFFFFDFAFFFFFF-@DBFFFFBFD---.;BAA. +@M01368:8:000000000-A3GHV:1:1109:19224:11655/1 +AAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGCATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCTGTCTCTTATACACATCTCCGAGCC ++ +BBB@A4FFFBBBGGGGGGGGGGHHHHHHHHFHHHHGFGHGGGFFAGFHHHFHHHGHHHHHHHHHHHFHGGHGHHGCFFGHHHGHHHGHHFHGGH3BFFGHHGHGEFFH/EG@-CFGGCC@-;BD?FGGGGGG??FGDAGDFFFFEFF@BAAFEFFBFFFFF?FD9BBFFFF/FFFFBFFFFFFF?FFBFFFF/FBBFFBFFFE?F;@B//AFFFFFFFFF//;BFFEF.BFFFB/;F/99/FBFFBDCF;B +@M01368:8:000000000-A3GHV:1:1109:21978:9490/1 +GGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCAGTT ++ +?A3AA?DFFFFFGGGGGGGFFGHFGCEGGFHHHHHHHHHGHCBGFEGGHHDEGAFGHGHFGDGBCGHHHHHFHHGHHHHHHHGHHHH1E?EGGGHHHHFGFHFHHHHHEDHHGHH@GGHGHHCGG?CFGFGGGGGFGB.BFGGFFGGFFFBDDBFFFFFF9-;DEFFDFBFFBB.EFF;BFBFFFFFFFFFFFFFFFFFFFFFFFFF/B9/FFFFFFFAFFFF--99AFFFFFFFA;;;FF..9//;/;;/ +@1:A:M01368:8:000000000-A3GHV:1:1109:22910:4079/1_PolyA:1 +TAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACAGTCCACTTTAGCT ++ +CCCCCFFFFFFFGGGGGGGGGGHHHHHHHHHHHHHHGGGGGHHHHGGEHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGFHHHGGGGGGGHGGGGGGHHHHHHHHHHHHHHHHHGGHHHGGHGGGHHGHGHHHHHHHGFHHHHHHHHHHHHHHHHHHGGHHHHHHHHHHGHHHHHHGGGGGGGGGGGGGGGGGGGGFFGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFCCDFFFFFFF0BFFFFFFF +@M01368:8:000000000-A3GHV:1:1109:24217:19077/1 +AAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGCCTTTGATTCCTGCCTCATCCTATTACTGTC ++ +>AAA3BDFFFFDGBFFEEGGGGHHGHFHHCFHGHGHHHGF3BFGFHGFEFHFHGH3EGFC1EEGBFGBHFGGFFHHHGHFHGHHGHGGHCEHEBCFF3ED3@GH4FDEG12FFHFFHG/G3FGA/@EFEFCG33FFGBBGHFHH/?BFBGFGFAGC2C/@-;@-;BFFEFADF?DFFFFFF?;AFFA;AD;..FEFFA--;9;D.9BFFBE?;.;///://///99//BB/9;F./B//BBFB9////99/ +@M01368:8:000000000-A3GHV:1:1109:25237:11722/1 +CCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT ++ +AABBBFFFFFBBF5BGGFGABGHGGAEFGFHHHHFHCHFHHBFGGGEHBGGHFFFFGFAEF5FHH?EGGF5AEG3?EBFHD?C/EFHFFEGEE?CFGGGHHFHB3EEFG?EEGHCFGGGGGGGEC<CDFHHHHHE.FD11FFAGBGD.DGHEA<.<<0<EHAHCFHHF./;CC0GFH0::0CFGG0FBFD;9/90BFF0CBBFGB09;B:0;CFF0.-;@AFEEF--;AFFBF/99D-;.//.../9;B-. +@M01368:8:000000000-A3GHV:1:1109:27103:7153/1 +GTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGCTGTCTC ++ +33>A?FFFDFBFGCG4EGFG??GACFGGHFHCFHCHBECDEFCEGHHHHHFFGGF3AFFHHFGEGCGHHGEHGFGHHEGG?EFHHH?EGGHHGGGGHHHHHHEGGGGGHFHHGFDDGGACFCC@?/FDDGHHHGHEFFHHH0FDHHFFEDCD.<GGEHGF0CHHHF/CAGC000CCGFHFGFGFFG?:CB990;F09;FB0BBGGGGGG?;A9EBDEFA///;FF/:F?BFBBFFBFFFF///;/9B;/// +@M01368:8:000000000-A3GHV:1:1109:8763:7859/1 +TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGCTTTATGC ++ +BABAADFFFFFFGGGGGGGGFBGFGHHFBHHHHGGHHGGEHGDHHHFGHEHHHHGHHGGGGAGHHFBHHGHFHHHHHHHHGGGCEFGFFFFEHHH?FFG1FFHHFEGGGHGFEEHHHHHGGGFGGGHHHHGGGGG/C/FG-<A<GFHHFFCGHHFHGE0<<CGHHHGCHG.CCCCCE00BBFGFFG.BB0FFBFBCF0;CBFFFEEGBBBFFBF/FFF9BF/9BAFFD9AFEDAA.A/BFFFFFFFFFEB/ +@M01368:8:000000000-A3GHV:1:1110:12808:18367/1 +AATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCCTG ++ +BBBBBFFFFBBFGGGGGGGGFGFGHHGGGGGHHHHHHHHGFHHGEGGGHHGFGHEEGGHHHHGHHGFFHHGGGGGHHHHGHGHGHHHFGGFHHGGGGGHHFFFGGHHHE?@EGGHFHEGGGHHGGGGHGEFHHGD?/>CFHHGDFGGGGGEFGC?EGHHHB0CGHHGCGHHHHBEHGHH.::ACA;FECFGGGGGFFFEFGGGGGGGFFBBBFGBFFFEFFFFBFFFFFFBBFBFFB9..AF?FFFFFFF9 +@M01368:8:000000000-A3GHV:1:1110:14751:26540/1 +TAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACTGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGTTGTGCACGCGATAGCATTGCGCGACGCTGGCGCCGGCGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTG ++ +AAA?1CFFFFADGEEGFGB1112DGDGHGFGHHCG/ECEBGGHDFGHHFHGHEB1BCGFFE2EGHHGHFFFHHBFFHEFCGGHHFHHHHH111BFFHGHEHBEHGGECEFHGFHFGGDBBGHHBHHHH0GH22FGHGFFFH1BFCG0F@FGG11@GDHFG1D/1FGFHH/FCG.>0><-<@CCFFG.CC-@C?C9FFF0C---;------;9----9-9@-AAFF9B9---9-/;B9BF/9BB//B/;/// +@M01368:8:000000000-A3GHV:1:1110:15519:10008/1 +CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACC ++ +BBBBBFFFFFFFGGGGGGCBGGE?GGGHHHHHGHHBEGGHHGGGGGHHHHHHHHHHHGGHHHHGFGGGGHGHGCFGHHHGGGCGGHGHGGGGGHFGGGHHHHHHGCEFGGHHHHGGGGGGGHGG/CEGHHHHGFHHHGFHHGFFHGHGHFGGEGFDDGGBCGHHGFHH/CHHHHGHGHF0CFGGGGGGGFFFFFBFFFFFFGFFGGF?DGEDEEFEFFFF?FFFFFFFFFFFFFFFBFFF.BFFFBFBBA; +@M01368:8:000000000-A3GHV:1:1110:16559:7507/1 +CATTCAATTGTTATTATTATGTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAACTGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATC ++ +ABBBAFFFFFFFFGGGGGGGFGGGGAGHHGCHHGHHHHHHHHHHHGHHGHHHHHHHHHHHHHHHHFGGGHHHHHHHHBDHHHHHGGHFEEGHGGHHHHHHHGHHHEHFGGGGGGHHHF33FGHHG3GDDGH//EEGHHFFBF?GHHFCC@DGFCGGB?DGHAFGGHHGFH0E??<@DGHGGCFGGF?DGGGFFGGGGGGGG.C/0BBF?FBBEGG0FFF.AA9;B9/E////99;;:.//:FFFFBBF//9 +@M01368:8:000000000-A3GHV:1:1110:16700:6843/1 +AAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCTGTCTCTTATACACATCTCCGAGCC ++ +BBBBBFFFFFFFGGGGGGGGGGHHHHGHHHHHHHHHHHHGGGGGHHHHHGGHGHGGGGGGHHHGFFGHHGGGGHHHHHHCGG@FGHFHFEGGGGGGHGGGGDGHHHHHHHHHHHHHHGHHGHGHHGGGGGCGHEHGHHHFHHHFF.DGHHHHHHGHHHHHHGFGHGCGHGHHHHHHHHGHHHCD?:A::FCDGEGFGFFBFGBFGGGF00CBFFFF/BFBFFFFAE.FBBFBFFFFB/BFFBFFF/ADCAD +@M01368:8:000000000-A3GHV:1:1110:18014:7856/1 +ATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGGGATAGACCTGTGATCCATCGTGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAAAAATAG ++ +AABBBFFFFFFFCGGGCGGGGGGHHHHHGFHHHHHHGHHHGGGGGFHHGHFGGHHHGGFGGGHHGGGGGHHGGGGHGGHHHGGGGGGHHGHGDGGGGGGGGB/A<GFHFHHHGHBBGFEGGGGHFFFEF/EEGGHGGFEHFHHBA.<ADFFHFHB/CCGHFEFHHGHGEFFGGGGGFFFGGFGFFBFFGGBFGG??DGG?@9-ADFFFFB/@BD;./BFE?BFE/>99FE//BBFFF.9/FF?DA=@9./9 +@M01368:8:000000000-A3GHV:1:1110:23303:11846/1 +ACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGTGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTC ++ +3ABB?FAB?DAAGGGFBGGBB5AFFFGFHHGGGEGHHHFHHF3EGFHHHHEFHHGGHHHGH3EFHHHHHBDGHHE?ECHGHGHHHHHHFHHHGHHHHHHHGGHGGFHHHFHHGFFHHFEGFHHHHHHEHHFDAGHHHH/BDDFGGH1FDBGHHHHHHHCGHHHFH?0CGCF>CC=@--AFBFFB?=BFDFFF//FDB;-9A-AD-.A.A=?-9-:B/AEB/BFF.A;DDFFFBBFFFF/BBFFFFBFF9B; +@1:A:M01368:8:000000000-A3GHV:1:1111:13195:14562/1_PolyA:1 +ATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGATACCTGTCTCTTATACACATCTCCGAGCCCACGAG ++ +ABAAAFFFFFFFGGGGGGGGGGGHHGG4FDFGFGGCGGGHGHG?EGHEGFFDHFHHHHGGFE@EHHGEEEGFCCEFFGFG/EHHHHHBFBGGHFHFHEHGHEFGGGED/EFDEFFEHHBGHHCFFFDFGGHHGFFFHHHHGFG>0<0DGGFHBGHFBDFGHGFFGGAGHHGCGBHFFHHHFFFBFGGGGF/:FFFCB9F9FF0FEFE?FF0BED-D.//BF9//BBFF/BFFFEFFFFED-DCFF?.:BF +@M01368:8:000000000-A3GHV:1:1111:14287:20817/1 +GCCAACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTCATCGCACCTACGTTCACTATTACAGGCGAACATACTTATTAAAGTGTGTTAATTAAT ++ +>>A3>AAAACFFFGFGFGGGGGHGHHHHHHHGGGGGHGHFFCGHHGBGHFHHHHAGHHHHEHHAGFFEHEAE?GHHHHEGFFFDGHGHBFFHFHHHGHGHGHBECG@@@EHGGEGCGGGGFFGGGG?BGGGGGG.-C.FEDCFFFEFFFAF/99BEFA.:9BFB/9FFFEFFFFFFEFFF/FBBFFFFFFFFFFFB//;.@@//;DFFFF//BBFFFBF;A;DF=>B//;9B//B/;/;9//9//;B/:B9 +@M01368:8:000000000-A3GHV:1:1111:23624:14516/1 +GTCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGGGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAG ++ +3>3AAFFFFFAFGGGGGGGGBGHHHHHHGGHGGHEHGHADFHHHHEGGGHBGHFFHHEHHGHFHGG5FBEEGEGHHHGHHHEFDGHHFFGFGGHHGHHHGHBHGHFHFHHGGGGFCFBGFGE?GFBECEGDGHEHGDGCGHGC/DF@GHGFFCGGGGFFFFDD?@C@GFEC?-ADHFFHBGHFBHFG0CC//.;B0/CA.;AA.;@.AB/9BB/A/.AFFFE/BFFF///9B/FBDE9/;99/B/BFFEF/ +@M01368:8:000000000-A3GHV:1:1111:7437:24508/1 +ACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTGTCTCTTATACACATCTCCGAGCCCACGAG ++ +CBCCDCFFFFFFGGGGGGGGGGGGGGHHHHHHHHHHHHHHHHGGGFFEHGGHHHHHHHHHHHGHHGGGGGHHHHHHFHHHHGHHHHGHGGGGGHHHHHGGHHHGGGGGGHHHGGGGHHGGGGHHHHHHGGGGGGHGHHGGGGGGGHGGGGGCGHHHHGHHGHHHHHHHEDHHGHGGGGGD;BEGGGFFGGGGC:CFGGGGGGGGGFGGGFFFGFFFFF;FFF;BFFFFFFFFFFFB:FFFFD=CAEEEF>- +@M01368:8:000000000-A3GHV:1:1111:8665:25542/1 +TCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTGTCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCG ++ +AA3AAFFFFFFFGGGFGFFGGGGHGH4AGGGFHHCGEE2FGHHHHHHHHHHGFHGF53FHEGGFAF5CFHHGGFGHFHHGGHHHCHHHHHHHFHHGH?EEEG1FFFEHHFHFGGHHGG?F4FBGHGHCHEF0//-9--;0CFGG?@BGDGFFFFEFBBBDA?FFBFFFF=B;-9.AA9EFFFF/;9BD.//;9/9/B:/:BFBFFBFFF/B:BFF//;/B/FFFBFF.A-BFFFBA/F//BFFFBF/;/A- +@1:A:M01368:8:000000000-A3GHV:1:1111:9655:13916/1_PolyA:1 +GGCCCATAACACTTGGGGGTAGCTAAAGTGAACTGTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGCGGTGTCCACGCAATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTG ++ +1>>1AAFFFFFBGGFE1EEEEGGHGHHH2FGHHHFGFGHGCFGGGEFHGGEGGHHHHGHEFBFEGHHHGHHBEHHHHGHGFHHHGGGGHHEGGGGHHHGFHGHHHH1FHHG?G/AGF1GBFGEFFHF2FDD0FG<GDHBFDGFFCGH//B//?0GFFGFGGHHHFHFB.=1FDFB?FGH.C/.-:::CH0;CAB@-.;0CGFFFGG--9.A@GB>--99@=---AAABA-BF/B?A@F-9BBFF//BB/9 +@M01368:8:000000000-A3GHV:1:1112:10272:7828/1 +GTATCCGACATCTGGTTCCTACTTCAGGGTCATAAAACCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT ++ +AAABABBBBBBFGGGGGGGGGGHGHHGHGHHHHHHHHHGHHHGGHDGHHHGGGGHHHHHHHHHHHHHHHFHGHHHHGGHGGHHHFHHHHHCGHFFHHHHHHHHHHHHHGHGEFGAEEEEHHGHHGHGHHHHHHHGHHHHHHGHGHFFBC/BA?BGGGFFFFFFFFFFFFFFFC-@DDFFFFFFFEFAFFFFFFBFBFFFFFFFFFFFFFFFFBFF->@FFFFFFFFFFBFBFAD--.99;A?////:.9A. +@M01368:8:000000000-A3GHV:1:1112:9832:16531/1 +CTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGGGCTGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCTTGAAGTAGGAACC ++ +AABABFFFFFFFF4GGGGEBGEFGHGGHGHGHHHGGHFBGFGGGGGHHEFHGEHFHFHEHHHHHGCGGGHFHHCFGHE@1EEEFFGB?EEEEHBGGGCHHGHHBEE@>EAHHFAEBDGGGCEFF?CEGFFHHHHHFHHHFHGHCGCAHHF<..C.D=0CD.GFHHHG.CAGHC:CHB0GGGFFB0:CFA.A-;9.;000CBBFFBFGA.9AA;A;EDDE.ABBFFFF9;BEFFF/BF//9/B//9:B//.. +@1:A:M01368:8:000000000-A3GHV:1:1112:9832:6701/1_PolyA:1 +CGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGG ++ +AABBBBFFFFFFGGGFGGGGGGHHHGGHGGHHHHHHHHHHHGHHGGGGGHHHHHHHHHHHHHHHHHHGGCGDHHHHFGGHHHGGGGGFGGHGGGGHHGGGGHHHHHHGGGGGGHHGGGGDGGFGHGGFCDCHHHHGHHHHGHHFAGFGGEHFGEG.C<EGCECEFCFFHBGHE:GHHGFF0FFFB0CFGGGGAGFFF0;;FFGGGFFGFFCDD.DEFBEDD?9FFFFFFFFFFFFFFF/B./FB///;BF +@M01368:8:000000000-A3GHV:1:1113:13532:8618/1 +GGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATGACCCTGAAGTAGGAACCAGATGTCGGCTACAGTTCACTTTAGCTACCTCCAAGTGTTATG ++ +AA?AAFFFFFFFGGGGGFBFGGGGGGH5GHFEDHHGGGGCGFBGH?AEEHFFEEGHGHHHHGGG?0EHGHHGGGGGC?GEE/EE?GHHHGBHFHHHHHHGHHGHHHGGGCG/A@<FGGHHHHHHHFF/GFDGHHHHHHGFFFGHGGHABGHFHHHHHFHEGDGHGDDEEHHBEDDEGFGHGBFFBFFEGGGEFGFGFB000;F0F;FGGEGGEFFF;-.9//B///B9FFFFF/BFFB/:.BB//;FBFFF +@M01368:8:000000000-A3GHV:1:1113:21679:18011/1 +GATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGAGGGTGTGCACGCGATAGCATTGCGAGACGCTTGAGCCGGAGCACCCTATGTCGCAGTATATGTCTTTGATTCCTGCCTCTTCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACTGTCTCTTATACACATATCCACGCCCACGAGACTAAGGCGAAGTTCATT ++ +AA>1>11F1D1DDFFFFGGG1FH3GHGHGHGHFHEC?EC0B1FBGFB1GHHH21AF2HH2FEGEHAE10AAE/>/FFHE?/E/?E1@EFFH?E@/EGGG//B11B//<@@C0F/<0<FFDGGCC?FF221<BDD11@<11@DDCGEF1<111F1<F1FBGHGHCF-CHHFA./</0CGHF<0CC/;C-:-;;09;FFBFBBFFBC0FFFGGFC0009C00090/-:--9--;-;AFFE;/99-9--/;/// +@M01368:8:000000000-A3GHV:1:1113:25528:14016/1 +CCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGG ++ +>AAAAFFFBFFFGGGGGGGGGGHHFHGHHHHGHHHHHHHHHHHGGGGHHHHFHHHHFHGHHHHGAFFEFHEGHHHHHHHHGHEHHGGFGGGHHHHHHFHHHHHGGHHHHGGGGGHFHHFF?HHGGFECEFFGHFFGFHGECDGHGBGFHGDF@@?CGFHCEGGGFD.CCC?EGHBHHHFHHFBCFFGEB/CEGGGGDAA.90C9CEBFGGBBF/9.9FBFFFBBFF//99FFFFEABF//99FFEFFFBFF +@1:A:M01368:8:000000000-A3GHV:1:1113:5741:16959/1_PolyA:1 +TAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGTGGGCTATTTAGGTTTTATG ++ +ABBBBFFFFFFFGGGFGGGGGGHHHGHHGGHBGHGAGFFCAFGHGFFGHHGFHHHHHGGGGGHGHHHHHHHHE3BFFHHHGG0EDF@GHFFGGGHGGGGGGGGGGGGGHHGGEEFHGFHHDDG@DGGGHHGDGGGGGHGG?CF?HHGHHHGHGHHHFFHGGGHHHHGGCD.;0<C;CGGGGEFF/.;0;FFFBF/0;0CFGFFB..9B/;0CBFFBBFFFFBAC?DED9;B9AD;.FFFB/B/;FBA/B/ +@1:A:M01368:8:000000000-A3GHV:1:1114:10130:11959/1_PolyA:1 +CGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTCTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGCAACAAACACACATCCAG ++ +>A33>FFFFFF@FFFGGGGFGGD4EGGGGGHHGFFGHFGGHHHFEFHECFHHHEHGAGBCCGCCEGGHGHEGFBFHFHHHHGGGHFHGHEGGGFEGEGG??@DBGHGGC-@FFGHFHFHB-??DA-DD@9:BEBG@B?E?A.ABBFBFA??F.FF/BFFB/B9B/9;BF9FF9FFFFFFFFFFFFFF?BB?;9EE;-;DDB9//;B-B@;ABFFEFFFF/-.9A.;//9/BF./B/..9.9:...///// +@M01368:8:000000000-A3GHV:1:1114:14540:5315/1 +CTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCGTGATGTCTTATTTAAGGGGAACGTGGGGGCTATTTAGGTTTTT ++ +AABCCFFFFFFFGGGGGGGGGGHHHHHHHFHHHHGHHGHHGGGHGGHHHHHHHGHHHHHHGGGGGHHFHHHFGHHGGFHHHHHGGGGGHHHGHGGHHHGGGGGGHGHGGGGHHGGGGHHHHHEGDDFGFFFHHGGGGGCDAFCFGFDHHHHGGHGHHHHHHBCGEHHHHGGHG.ACGEHGG0CBFFF:A;BB0;09CGF00CFFFE0AA?//CFFFFFFFFFFFFFFFBEF;A.-=A--:BBFB90;;FE. +@1:A:M01368:8:000000000-A3GHV:1:1114:15066:16302/1_PolyA:1 +TAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGTGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTTCAATATTACAGGCGAACATACTTACTAACGGTTGTTAATTAATTATTGCTTGTAGGAC ++ +BBBBAFFBDFBFBGGGGGFGGGBGFFFHGFHHGFFFHGHHHGHHHHFFHHHGHGC?AEFFHEFBFFFGHHHHH1FDGFFHGHGHFEGCGC-<<AHHHGGGGGGGFHH0GHFCCCADGGG?.9/A-???DGGFFF.9F9/EE-;;BBBFFBFFFFFFFFFEFFFFBFFBBFFFFF/BFFBFFFFF-DBFFF;/BFF//BB//9/BEA---9:BFFFFFF/F/.;.:@9.BBFF/;BFF/;/////9///// +@M01368:8:000000000-A3GHV:1:1114:16639:15258/1 +CCTAAATAGCCCACACGTTCCCCTTAAATAAGACATCACGATGGATCACAGGTCTATCACCCTATTAACCACTCACGGGAGCTCTCCATGCATTTGGTATTTTCGTCTGGGGGGTGCGCACGCGATAGCATTGCGAGACGCTGGAGCCGGAGCACCCTATGTCGCAGTATCTGTCTTTGATTCCTGCCTCATCCTATTATTTATCGCACCTACGTCCAATATTACAGGCGAACATACTTACTAAATTGTGT ++ +11>>ABFFFFFFGGCGC1BBF1GHHHBHHHHGFHGH1A100AA/GGEHGFBAA1FFD2FHHHHECG2F1BB/E0FC/A>EE/FGGFGEF1EGBBEHHCGGGHBGEHFHE0B?E--;C?CCGGG@A@GBFBBBB09?@??A-AB--ABE@=-=-9AE9A;FFFFFE=9-//;//;9FF/////;;///9B///;/B////9FFBB;--A@-;/9;9-:-/;;FFFE9BF?@;-9-99/B9F/://///99/; +@M01368:8:000000000-A3GHV:1:1114:2404:13066/1 +TCCTACAAGCATTAATTAATTAACACACTTTAGTAAGTATGTTCGCCTGTAATATTGAACGTAGGTGCGATAAATAATAGGATGAGGCAGGAATCAAAGACAGATACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTGCACACCCCCCAGACGAAAATACCAAATGCATGGAGAGCTCCCGTGAGTGGTTAATAGGGTGATAGACCTGTGATCCATCTGTCTATTATACACATC ++ +CCCCCFFFFCFFGGGGGGGGGGHHHHHGHHHHHHHHHFFHHHHHGGGGHHHHHHHHFHHHHHHFGGHHGGHGGHHHHHHGHHFHHHHGGGGGGHHHHHHGHHHHHHHHHHGGGGGGGHH?FGHHHGGGGGGHHGGFGGHHGGGGHHHHHFGGGGFGHGHHGGGGGGGHGGGEGGHHGHHHHHHHHHGFBFFDA0FGGGFFGG0:EFGGGGGGGG;AEBF0B0BFFBFFFFFFFFFFFFFFFFFFFFFEFF0
--- a/trim_galore.xml Tue Jul 30 06:26:49 2019 -0400 +++ b/trim_galore.xml Fri Oct 08 09:57:52 2021 +0000 @@ -1,50 +1,10 @@ -<tool id="trim_galore" name="Trim Galore!" version="0.6.3" profile="17.01"> - <!-- Wrapper compatible with Trim Galore! version 0.6.3 --> +<tool id="trim_galore" name="Trim Galore!" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="20.01"> <description>Quality and adapter trimmer of reads</description> <macros> - <macro name="adapter_trimming"> - <conditional name="trimming"> - <param name="trimming_select" type="select" label="Adapter sequence to be trimmed"> - <option value="">Automatic detection</option> - <option value="--illumina">Illumina universal</option> - <option value="--nextera">Nextera transposase</option> - <option value="--small_rna">Illumina small RNA adapters</option> - <option value="user">User defined adapter sequence</option> - </param> - <when value=""/> - <when value="--illumina"/> - <when value="--nextera"/> - <when value="--small_rna"/> - <when value="user"> - <param name="adapter" type="text" value="AGATCGGAAGAGC" label="Adapter sequence to be trimmed off"> - <validator type="regex" message="Adapter sequence must contain DNA characters only (A,C,T,G or N)">^[ACTGNactgn]*$</validator> - </param> - <yield/> - </when> - </conditional> - </macro> - <macro name="paired_adapter_trimming"> - <expand macro="adapter_trimming"> - <param name="adapter2" type="text" optional="True" value="" label="Adapter sequence to be trimmed off read 2"> - <validator type="regex" message="Adapter sequence must contain DNA characters only (A,C,T,G or N)">^[ACTGNactgn]*$</validator> - </param> - </expand> - <param name="trim1" type="boolean" truevalue="--trim1" falsevalue="" checked="False" label="Trims 1 bp off every read from its 3' end." help="" /> - <param name="three_prime_clip_R1" type="integer" value="" optional="True" label="Remove N bp from the 3' end of read 1"> - <help>Instructs Trim Galore! to remove N bp from the 3' end of read 1 after adapter/quality trimming has been performed. - This may remove some unwanted bias from the 3' end that is not directly related to adapter sequence or basecall quality. - (--three_prime_clip_R1)</help> - </param> - <param name="three_prime_clip_R2" type="integer" value="" optional="True" label="Remove N bp from the 3' end of read 2"> - <help>Instructs Trim Galore! to remove N bp from the 3' end of read 2 after - adapter/quality trimming has been performed. This may remove some unwanted bias from - the 3' end that is not directly related to adapter sequence or basecall quality.</help> - </param> - </macro> + <import>macros.xml</import> </macros> - <requirements> - <requirement type="package" version="0.6.3">trim-galore</requirement> - </requirements> + <expand macro="requirements" /> + <expand macro="xrefs"/> <version_command> trim_galore --version </version_command> @@ -92,6 +52,9 @@ trim_galore + ## according the develpers 4 cores could be a sweet spot, anything above has diminishing returns + --cores \${GALAXY_SLOTS:-4} + ## we only support fastqsanger --phred33 @@ -177,6 +140,18 @@ --dont_gzip #end if + ## Trimming settings + #if $trimming.settingsType == 'custom' + #if $trimming.hardtrim5 + --hardtrim5 $trimming.hardtrim5 + #end if + #if $trimming.hardtrim3 + --hardtrim3 $trimming.hardtrim3 + #end if + $trimming.clock + $trimming.polyA + #end if + ## Trim Galore is finished, rename the output if compressed && if [ -f input_1_trimmed.fq.gz ] ; then mv input_1_trimmed.fq.gz input_1_trimmed.fq ; fi @@ -188,12 +163,37 @@ if [ -f input_1_unpaired_1.fq.gz ] ; then mv input_1_unpaired_1.fq.gz input_1_unpaired_1.fq ; fi && if [ -f input_2_unpaired_2.fq.gz ] ; then mv input_2_unpaired_2.fq.gz input_2_unpaired_2.fq ; fi + && + if [ -f input_1.clock_UMI.R1.fq.gz ] ; then mv input_1.clock_UMI.R1.fq.gz input_1.clock_UMI.R1.fq ; fi + && + if [ -f input_2.clock_UMI.R2.fq.gz ] ; then mv input_2.clock_UMI.R2.fq.gz input_2.clock_UMI.R2.fq ; fi + + ## Rename hardtrimmed files + #if $trimming.settingsType == 'custom' + && + if [ -f input_1.${trimming.hardtrim5}bp_5prime.fq.gz ] ; then mv input_1.${trimming.hardtrim5}bp_5prime.fq.gz input_1_hardtrim.fq ; fi + && + if [ -f input_2.${trimming.hardtrim5}bp_5prime.fq.gz ] ; then mv input_2.${trimming.hardtrim5}bp_5prime.fq.gz input_2_hardtrim.fq ; fi + && + if [ -f input_1.${trimming.hardtrim3}bp_3prime.fq.gz ] ; then mv input_1.${trimming.hardtrim3}bp_3prime.fq.gz input_1_hardtrim.fq ; fi + && + if [ -f input_2.${trimming.hardtrim3}bp_3prime.fq.gz ] ; then mv input_2.${trimming.hardtrim3}bp_3prime.fq.gz input_2_hardtrim.fq ; fi + && + if [ -f input_1.${trimming.hardtrim5}bp_5prime.fq ] ; then mv input_1.${trimming.hardtrim5}bp_5prime.fq input_1_hardtrim.fq ; fi + && + if [ -f input_2.${trimming.hardtrim5}bp_5prime.fq ] ; then mv input_2.${trimming.hardtrim5}bp_5prime.fq input_2_hardtrim.fq ; fi + && + if [ -f input_1.${trimming.hardtrim3}bp_3prime.fq ] ; then mv input_1.${trimming.hardtrim3}bp_3prime.fq input_1_hardtrim.fq ; fi + && + if [ -f input_2.${trimming.hardtrim3}bp_3prime.fq ] ; then mv input_2.${trimming.hardtrim3}bp_3prime.fq input_2_hardtrim.fq ; fi + #end if ## Trim Galore! run is finished. Move the report files to the proper place #if $params.settingsType == "custom" and $params.report: && cat ./*_trimming_report.txt > '$report_file' #end if + && ls -lah ]]></command> <inputs> <!-- Input Parameters --> @@ -226,7 +226,7 @@ </conditional> <conditional name="params"> - <param name="settingsType" type="select" label="Trim Galore! advanced settings" help="You can use the default settings or set custom values for any of Trim Galore!'s parameters."> + <param name="settingsType" type="select" label="Advanced settings" help="You can use the default settings or set custom values for any of Trim Galore!'s parameters."> <option value="default">Use defaults</option> <option value="custom">Full parameter list</option> </param> @@ -261,7 +261,7 @@ </conditional> <!-- params --> <conditional name="rrbs"> - <param name="settingsType" type="select" label="RRBS specific settings"> + <param name="settingsType" type="select" label="RRBS specific settings" help="You can use the default settings or set custom values for any of Trim Galore!'s parameters."> <option value="default">Use defaults (no RRBS)</option> <option value="custom">Full parameter list</option> </param> @@ -274,35 +274,84 @@ label="Screen quality-trimmed sequences for 'CAA' or 'CGA' at the start of the read and, if found, removes the first two basepairs" /> </when> <!-- full --> </conditional> <!-- params --> + <!--Trimming options--> + <conditional name="trimming"> + <param name="settingsType" type="select" label="Trimming settings" help="You can use the default settings or set custom values for any of Trim Galore!'s parameters."> + <option value="default">Use defaults</option> + <option value="custom">Full parameter list</option> + </param> + <when value="default" /> + <when value="custom"> + <param argument="--hardtrim5" type="integer" min="1" optional="true" label="Hard-trimm 5' ends" help="Instead of performing adapter-/quality trimming, this option will simply hard-trim sequences to N bp at the 5'-end. Once hard-trimming of files is complete, it will exit" /> + <param argument="--hardtrim3" type="integer" min="1" optional="true" label="Hard-trimm 3' ends" help="Instead of performing adapter-/quality trimming, this option will simply hard-trim sequences to N bp at the 3'-end. Once hard-trimming of files is complete, it will exit" /> + <param argument="--clock" type="boolean" truevalue="--clock" falsevalue="" label="Mouse epigenetic clock mode" help="In this mode, reads are trimmed in a specific way that is currently used for the Mouse Epigenetic Clock"/> + <param argument="--polyA" type="boolean" truevalue="--polyA" falsevalue="" label="Remove polyA tails" help="This is a new, still experimental, trimming mode to identify and remove poly-A tails from sequences" /> + </when> + </conditional> </inputs> <outputs> <data format_source="input_singles" name="trimmed_reads_single" from_work_dir="input_1_trimmed.fq" label="${tool.name} on ${on_string}: trimmed reads"> <filter>singlePaired['sPaired'] == "single"</filter> + <filter>trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''</filter> </data> + <data format_source="input_singles" name="hardtrim_reads_single" from_work_dir="input_1_hardtrim.fq" label="${tool.name} on ${on_string}: hard-trimmed reads"> + <filter>singlePaired['sPaired'] == "single"</filter> + <filter>trimming['settingsType'] == "custom"</filter> + <filter>trimming['hardtrim3'] != '' or trimming['hardtrim5'] != ''</filter> + </data> + + <!--Trimmed reads paired collection--> <collection name="trimmed_reads_paired_collection" type="paired" label="${tool.name} on ${on_string}: paired reads"> <data name="forward" format_source="input_mate_pairs['forward']" from_work_dir="input_1_val_1.fq" /> <data name="reverse" format_source="input_mate_pairs['forward']" from_work_dir="input_2_val_2.fq" /> <filter>singlePaired['sPaired'] == "paired_collection"</filter> + <filter>trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''</filter> + <filter>trimming['clock'] == False</filter> </collection> - + + <!--Unpaired reads collection--> <collection name="trimmed_reads_unpaired_collection" type="paired" label="${tool.name} on ${on_string}: unpaired reads"> <data name="forward" format_source="input_mate_pairs['forward']" from_work_dir="input_1_unpaired_1.fq" /> <data name="reverse" format_source="input_mate_pairs['forward']" from_work_dir="input_2_unpaired_2.fq" /> <filter>params['settingsType'] == "custom"</filter> <filter>params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output"</filter> <filter>singlePaired['sPaired'] == "paired_collection"</filter> + <filter>trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''</filter> </collection> + <!--Hard-trimmed reads paired collection--> + <collection name="hardtrimmed_reads_paired_collection" type="paired" label="${tool.name} on ${on_string}: hard-trimmed paired reads"> + <data name="forward" format_source="input_mate_pairs['forward']" from_work_dir="input_1_hardtrim.fq" /> + <data name="reverse" format_source="input_mate_pairs['forward']" from_work_dir="input_2_hardtrim.fq" /> + <filter>singlePaired['sPaired'] == "paired_collection"</filter> + <filter>trimming['settingsType'] == "custom"</filter> + <filter>trimming['hardtrim3'] or trimming['hardtrim5']</filter> + </collection> + + <!--Mouse epigenetic reads paired collection--> + <collection name="mouse_reads_paired_collection" type="paired" label="${tool.name} on ${on_string}: MEC paired reads"> + <data name="forward" format_source="input_mate_pairs['forward']" from_work_dir="input_1.clock_UMI.R1.fq" /> + <data name="reverse" format_source="input_mate_pairs['forward']" from_work_dir="input_2.clock_UMI.R2.fq" /> + <filter>singlePaired['sPaired'] == "paired_collection"</filter> + <filter>trimming['settingsType'] == "custom"</filter> + <filter>trimming['clock']</filter> + </collection> + + <data format_source="input_mate1" name="trimmed_reads_pair1" from_work_dir="input_1_val_1.fq" label="${tool.name} on ${on_string}: trimmed reads pair 1"> <filter>singlePaired['sPaired'] == "paired"</filter> + <filter>trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''</filter> + <filter>trimming['clock'] == False</filter> </data> <data format_source="input_mate2" name="trimmed_reads_pair2" from_work_dir="input_2_val_2.fq" label="${tool.name} on ${on_string}: trimmed reads pair 2"> <filter>singlePaired['sPaired'] == "paired"</filter> + <filter>trimming['hardtrim3'] == '' and trimming['hardtrim5'] == ''</filter> + <filter>trimming['clock'] == False</filter> </data> <data format_source="input_mate1" name="unpaired_reads_1" from_work_dir="input_1_unpaired_1.fq" @@ -318,6 +367,36 @@ <filter>params['retain_unpaired']['retain_unpaired_select'] == "retain_unpaired_output"</filter> <filter>singlePaired['sPaired'] == "paired"</filter> </data> + <!--Hard-trimmed paired reads--> + <data format_source="input_mate1" name="hardtrimmed_reads_pair1" from_work_dir="input_1_hardtrim.fq" + label="${tool.name} on ${on_string}: hard-trimmed reads pair 1"> + <filter>singlePaired['sPaired'] == "paired"</filter> + <filter>trimming['settingsType'] == 'custom'</filter> + <filter>trimming['hardtrim3'] or trimming['hardtrim5']</filter> + </data> + + <data format_source="input_mate2" name="hardtrimmed_reads_pair2" from_work_dir="input_2_hardtrim.fq" + label="${tool.name} on ${on_string}: hard-trimmed reads pair 2"> + <filter>singlePaired['sPaired'] == "paired"</filter> + <filter>trimming['settingsType'] == 'custom'</filter> + <filter>trimming['hardtrim3'] or trimming['hardtrim5']</filter> + </data> + + <!--Mouse epigenetic mode paired reads--> + <data format_source="input_mate1" name="mec_reads_pair1" from_work_dir="input_1.clock_UMI.R1.fq" + label="${tool.name} on ${on_string}: MEC reads pair 1"> + <filter>singlePaired['sPaired'] == "paired"</filter> + <filter>trimming['settingsType'] == 'custom'</filter> + <filter>trimming['clock']</filter> + </data> + + <data format_source="input_mate2" name="mec_reads_pair2" from_work_dir="input_2.clock_UMI.R2.fq" + label="${tool.name} on ${on_string}: MEC reads pair 2"> + <filter>singlePaired['sPaired'] == "paired"</filter> + <filter>trimming['settingsType'] == 'custom'</filter> + <filter>trimming['clock']</filter> + </data> + <data format="txt" name="report_file" label="${tool.name} on ${on_string}: report file"> <filter>params['settingsType'] == "custom"</filter> <filter>params['report'] == True</filter> @@ -325,50 +404,50 @@ </outputs> <tests> - <test> + <test expect_num_outputs="2"> <param name="input_singles" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> <param name="sPaired" value="single" /> <param name="settingsType" value="custom" /> <param name="report" value="true" /> <output name="trimmed_reads_single" file="sanger_full_range_results1.fastqsanger" ftype="fastqsanger"/> - <output name="report_file" file="sanger_full_range_report_results1.txt" ftype="txt" lines_diff="8" /> + <output name="report_file" file="sanger_full_range_report_results1.txt" ftype="txt" lines_diff="12" /> </test> - <test> + <test expect_num_outputs="2"> <param name="input_singles" value="sanger_full_range_original_sanger.fastq.gz" ftype="fastqsanger.gz" /> <param name="sPaired" value="single" /> <param name="settingsType" value="custom" /> <param name="report" value="true" /> <output name="trimmed_reads_single" file="sanger_full_range_results1.fastq.gz" ftype="fastqsanger.gz" decompress="true" /> - <output name="report_file" file="sanger_full_range_report_results1gz.txt" ftype="txt" lines_diff="9" /> + <output name="report_file" file="sanger_full_range_report_results1gz.txt" ftype="txt" lines_diff="12" /> </test> - <test> + <test expect_num_outputs="1"> <param name="input_singles" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> <param name="sPaired" value="single" /> <param name="trimming_select" value="--illumina" /> <output name="trimmed_reads_single" file="sanger_full_range_results2.fastqsanger" ftype="fastqsanger"/> </test> - <test> + <test expect_num_outputs="1"> <param name="input_singles" value="sanger_full_range_original_sanger.fastq.gz" ftype="fastqsanger.gz" /> <param name="sPaired" value="single" /> <param name="trimming_select" value="--illumina" /> <output name="trimmed_reads_single" file="sanger_full_range_results2.fastq.gz" ftype="fastqsanger.gz" decompress="true" /> </test> - <test> + <test expect_num_outputs="1"> <param name="input_singles" value="sanger_full_range_original_sanger.fastqsanger" ftype="fastqsanger" /> <param name="sPaired" value="single" /> <param name="adapter" value="AAAGAGC" /> <output name="trimmed_reads_single" file="sanger_full_range_results3.fastqsanger" ftype="fastqsanger"/> </test> - <test> + <test expect_num_outputs="1"> <param name="input_singles" value="sanger_full_range_original_sanger.fastq.gz" ftype="fastqsanger.gz" /> <param name="sPaired" value="single" /> <param name="adapter" value="AAAGAGC" /> <output name="trimmed_reads_single" file="sanger_full_range_results3.fastq.gz" ftype="fastqsanger.gz" decompress="true" /> </test> - <test> + <test expect_num_outputs="3"> <param name="input_mate1" value="bwa-mem-fastq1.fq" ftype="fastqsanger" /> <param name="input_mate2" value="bwa-mem-fastq2.fq" ftype="fastqsanger" /> <param name="sPaired" value="paired" /> @@ -378,7 +457,7 @@ <output name="trimmed_reads_pair2" file="paired_example_pair2_results2.fastqsanger" ftype="fastqsanger"/> <output name="report_file" file="paired_example_results2.txt" ftype="txt" lines_diff="24" /> </test> - <test> + <test expect_num_outputs="3"> <param name="input_mate1" value="bwa-mem-fastq1.fq.gz" ftype="fastqsanger.gz" /> <param name="input_mate2" value="bwa-mem-fastq2.fq.gz" ftype="fastqsanger.gz" /> <param name="sPaired" value="paired" /> @@ -389,7 +468,7 @@ <output name="report_file" file="paired_example_results2gz.txt" ftype="txt" lines_diff="24" /> </test> - <test> + <test expect_num_outputs="7"> <param name="input_mate_pairs"> <collection type="paired"> <element name="forward" value="bwa-mem-fastq1.fq" ftype="fastqsanger" /> @@ -414,7 +493,7 @@ <element name="reverse" file="paired_collection_example_unpair2_results3.fastqsanger" ftype="fastqsanger"/> </output_collection> </test> - <test> + <test expect_num_outputs="7"> <param name="input_mate_pairs"> <collection type="paired"> <element name="forward" value="bwa-mem-fastq1.fq.gz" ftype="fastqsanger.gz" /> @@ -439,6 +518,54 @@ <element name="reverse" file="paired_collection_example_unpair2_results3.fastq.gz" ftype="fastqsanger.gz" decompress="true" /> </output_collection> </test> + <!--Test hard-trim option--> + <test expect_num_outputs="2"> + <param name="input_mate1" value="bwa-mem-fastq1.fq" ftype="fastqsanger" /> + <param name="input_mate2" value="bwa-mem-fastq2.fq" ftype="fastqsanger" /> + <param name="sPaired" value="paired" /> + <conditional name="trimming"> + <param name="settingsType" value="custom" /> + <param name="hardtrim3" value="20"/> + </conditional> + <output name="hardtrimmed_reads_pair1" file="paired_hardtrimmed3_pair1_.fastqsanger" ftype="fastqsanger"/> + <output name="hardtrimmed_reads_pair1" file="paired_hardtrimmed3_pair2_.fastqsanger" ftype="fastqsanger"/> + </test> + <test expect_num_outputs="2"> + <param name="input_mate1" value="bwa-mem-fastq1.fq" ftype="fastqsanger" /> + <param name="input_mate2" value="bwa-mem-fastq2.fq" ftype="fastqsanger" /> + <param name="sPaired" value="paired" /> + <conditional name="trimming"> + <param name="settingsType" value="custom" /> + <param name="hardtrim5" value="20"/> + </conditional> + <output name="hardtrimmed_reads_pair1" file="paired_hardtrimmed5_pair1_.fastqsanger" ftype="fastqsanger"/> + <output name="hardtrimmed_reads_pair1" file="paired_hardtrimmed5_pair2_.fastqsanger" ftype="fastqsanger"/> + </test> + + <!--Test mouse epigenetic clock option--> + <test expect_num_outputs="2"> + <param name="input_mate1" value="bwa-mem-fastq1.fq" ftype="fastqsanger" /> + <param name="input_mate2" value="bwa-mem-fastq2.fq" ftype="fastqsanger" /> + <param name="sPaired" value="paired" /> + <conditional name="trimming"> + <param name="settingsType" value="custom" /> + <param name="clock" value="true"/> + </conditional> + <output name="mec_reads_pair1" file="mec_reads_pair1.fastqsanger" ftype="fastqsanger"/> + <output name="mec_reads_pair2" file="mec_reads_pair2.fastqsanger" ftype="fastqsanger"/> + </test> + <!--Test polyA option--> + <test expect_num_outputs="2"> + <param name="input_mate1" value="bwa-mem-fastq1.fq" ftype="fastqsanger" /> + <param name="input_mate2" value="bwa-mem-fastq2.fq" ftype="fastqsanger" /> + <param name="sPaired" value="paired" /> + <conditional name="trimming"> + <param name="settingsType" value="custom" /> + <param name="polyA" value="true"/> + </conditional> + <output name="trimmed_reads_pair1" file="trimmed_polyA_reads_pair1.fastqsanger" ftype="fastqsanger"/> + <output name="trimmed_reads_pair1" file="trimmed_polyA_reads_pair2.fastqsanger" ftype="fastqsanger"/> + </test> </tests> <help><![CDATA[ **What it does** @@ -452,7 +579,6 @@ * Trim Galore! can remove sequences if they become too short during the trimming process. For paired-end files Trim Galore! removes entire sequence pairs if one (or both) of the two reads became shorter than the set length cutoff. Reads of a read-pair that are longer than a given threshold but for which the partner read has become too short can optionally be written out to single-end files. This ensures that the information of a read pair is not lost entirely if only one read is of good quality * Trim Galore! can trim paired-end files by 1 additional bp from the 3' end of all reads to avoid problems with invalid alignments with Bowtie 1 -.. _Trim Galore!: http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ It is developed by Felix Krueger at the Babraham Institute. @@ -591,6 +717,54 @@ | Selecting this option for non-directional RRBS libraries will screen quality-trimmed sequences for 'CAA' or 'CGA' at the start of the read and, if found, removes the first two basepairs. Like with the option '--rrbs' this avoids using cytosine positions that were filled-in during the end-repair step. '--non_directional' requires '--rrbs' to be specified as well. | | *option --non_directional* + +---- + +**Trim specific seetings** + +* **Hard-trimming mode** + + | Instead of performing adapter-/quality trimming, this option will simply hard-trim sequences to N bp at the 5' or 3' -end. + | + | *options -hardtrim5 and -hardtrim3* + +* **Mouse Epigenetic Clock mode** + + | In this mode, reads are trimmed in a specific way that is currently used for the Mouse Epigenetic Clock (see here: `Multi-tissue DNA methylation age predictor in mouse`_). Following this, Trim Galore will exit. + | + | In it's current implementation, the dual-UMI RRBS reads come in the following format: + | + | Read 1 5' UUUUUUUU CAGTA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF TACTG UUUUUUUU 3' + | Read 2 3' UUUUUUUU GTCAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF ATGAC UUUUUUUU 5' + | + | Where UUUUUUUU is a random 8-mer unique molecular identifier (UMI), CAGTA is a constant region, and FFFFFFF... is the actual RRBS-Fragment to be sequenced. The UMIs for Read 1 (R1) and Read 2 (R2), as well as the fixed sequences (F1 or F2), are written into the read ID and removed from the actual sequence. + | + | *option --clock* + +* **PolyA mode** + + | This is a new, still experimental, trimming mode to identify and remove poly-A tails from sequences. When selected, Trim Galore attempts to identify from the first supplied sample whether sequences contain more often a stretch of either 'AAAAAAAAAA' or 'TTTTTTTTTT'. This determines if Read 1 of a paired-end end file, or single-end files, are trimmed for PolyA or PolyT. In case of paired-end sequencing, Read2 is trimmed for the complementary base from the start of the reads. + | + | PLEASE NOTE: The poly-A trimming mode expects that sequences were both adapter and quality trimmed before looking for Poly-A tails, and it is the user's responsibility to carry out an initial round of trimming. + | + | *option --polyA* + +* **Amplicon mode** + + | This is a special mode of operation for paired-end data, such as required for the IMPLICON method, where a UMI sequence is getting transferred from the start of Read 2 to the readID of both reads. Following this, Trim Galore will exit. + | + | In it's current implementation, the UMI carrying reads come in the following format: + | + | Read 1 5' FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF 3' + | Read 2 3' UUUUUUUUFFFFFFFFFFFFFFFFFFFFFFFFFFFF 5' + | + | Where UUUUUUUU is a random 8-mer unique molecular identifier (UMI) and FFFFFFF... is the actual fragment to be sequenced. + | + | *option --amplicon* + +.. _Trim Galore!: http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ +.. _Multi-tissue DNA methylation age predictor in mouse: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1203-5 + ]]></help> - <citations></citations> + <expand macro="citations" /> </tool>