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1 #!/usr/bin/env python
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2
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3 """
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4 Converts tRNAScan output back to fasta-sequences.
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5 """
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6 import sys
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7 from Bio import SeqIO
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8 from Bio.SeqRecord import SeqRecord
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9 import subprocess
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10
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11
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12 def main(args):
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13 """
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14 Call from galaxy:
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15 tRNAscan.py $organism $mode $showPrimSecondOpt $disablePseudo $showCodons $tabular_output $inputfile $fasta_output
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16
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17 tRNAscan-SE $organism $mode $showPrimSecondOpt $disablePseudo $showCodons -d -Q -y -q -b -o $tabular_output $inputfile;
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18 """
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19 cmd = """tRNAscan-SE -Q -y -q -b %s""" % ' '.join( args[:-1] )
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20 child = subprocess.Popen(cmd.split(),
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21 stdout=subprocess.PIPE, stderr=subprocess.PIPE)
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22 stdout, stderr = child.communicate()
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23 return_code = child.returncode
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24 if return_code:
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25 sys.stdout.write(stdout)
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26 sys.stderr.write(stderr)
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27 sys.stderr.write("Return error code %i from command:\n" % return_code)
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28 sys.stderr.write("%s\n" % cmd)
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29 else:
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30 sys.stdout.write(stdout)
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31 sys.stdout.write(stderr)
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32
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33 outfile = args[-1]
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34 sequence_file = args[-2]
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35 tRNAScan_file = args[-3]
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36
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37 with open( sequence_file ) as sequences:
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38 sequence_recs = SeqIO.to_dict(SeqIO.parse(sequences, "fasta"))
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39
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40 tRNAs = []
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41 with open(tRNAScan_file) as tRNA_handle:
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42 for line in tRNA_handle:
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43 line = line.strip()
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44 if not line or line.startswith('#'):
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45 continue
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46 cols = line.split()
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47 iid = cols[0].strip()
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48 start = int(cols[2])
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49 end = int(cols[3])
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50 aa = cols[4]
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51 codon = cols[5]
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52 rec = sequence_recs[ iid ]
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53 if start > end:
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54 new_rec = rec[end:start]
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55 new_rec.seq = new_rec.seq.reverse_complement()
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56 new_rec.description = "%s %s %s %s %s" % (rec.description, aa, codon, start, end)
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57 new_rec.id = rec.id
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58 new_rec.name = rec.name
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59 tRNAs.append( new_rec )
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60 else:
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61 new_rec = rec[start:end]
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62 new_rec.id = rec.id
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63 new_rec.name = rec.name
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64 new_rec.description = "%s %s %s %s %s" % (rec.description, aa, codon, start, end)
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65 tRNAs.append( new_rec )
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66
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67 SeqIO.write(tRNAs, open(outfile, 'w+'), "fasta")
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68
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69
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70 if __name__ == '__main__':
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71 main(sys.argv[1:])
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