annotate aragorn.xml @ 2:358f58401cd6 draft default tip

planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/trna_prediction commit cfb19d75629f02e0dea4475c16c016ed5510eb44
author bgruening
date Wed, 26 Jul 2017 10:14:05 -0400
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1 <tool id="aragorn_trna" name="tRNA and tmRNA" version="0.6">
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2 <description>prediction (Aragorn)</description>
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3 <requirements>
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4 <requirement type="package" version="1.2.36">aragorn</requirement>
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5 <requirement type="package" version="2.7">python</requirement>
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6 </requirements>
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7 <command><![CDATA[
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8 #if not $gff3_output:
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9 aragorn
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10 '$input'
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11 -gc$genbank_gencode
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12 $tmRNA
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13 $tRNA
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14 $mtRNA
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15 $mam_mtRNA
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16 $topology
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17 -o '$output'
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18 $secondary_structure
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19 $introns
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20 #end if
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21
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22 #if $gff3_output:
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23 aragorn
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24 '$input'
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25 -gc$genbank_gencode
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26 $tmRNA
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27 $tRNA
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28 $mtRNA
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29 $mam_mtRNA
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30 $topology
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31 $introns
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32 -w
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33 | python '$__tool_directory__/aragorn_out_to_gff3.py' $gff3_model > '$gff3_output_file'
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34 #end if
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35 ]]>
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36 </command>
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37 <inputs>
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38 <param name="input" type="data" format="fasta" label="Genome Sequence"/>
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39 <param name="genbank_gencode" type="select" label="Genetic code">
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40 <option value="1" selected="true">1. Standard</option>
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41 <option value="2">2. Vertebrate Mitochondrial</option>
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42 <option value="3">3. Yeast Mitochondrial</option>
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43 <option value="4">4. Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option>
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44 <option value="5">5. Invertebrate Mitochondrial</option>
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45 <option value="6">6. Ciliate, Dasycladacean and Hexamita Nuclear Code</option>
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46 <option value="9">9. Echinoderm Mitochondrial</option>
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47 <option value="10">10. Euplotid Nuclear</option>
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48 <option value="11">11. Bacteria and Archaea</option>
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49 <option value="12">12. Alternative Yeast Nuclear</option>
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50 <option value="13">13. Ascidian Mitochondrial</option>
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51 <option value="14">14. Flatworm Mitochondrial</option>
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52 <option value="15">15. Blepharisma Macronuclear</option>
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53 <option value="16">16. Chlorophycean Mitochondrial</option>
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54 <option value="21">21. Trematode Mitochondrial</option>
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55 <option value="22">22. Scenedesmus obliquus mitochondrial</option>
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56 <option value="23">23. Thraustochytrium Mitochondrial</option>
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57 <option value="24">24. Pterobranchia mitochondrial</option>
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58 </param>
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59 <param name="topology" type="select" label="Topology">
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60 <option value="-c">Assume that each sequence has a circular topology</option>
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61 <option value="-l">Assume that each sequence has a linear topology</option>
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62 </param>
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63 <param name='tmRNA' type='boolean' label='Search for tmRNA genes'
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64 truevalue='-m' falsevalue='' checked="true" help='(-m)' />
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65 <param name='tRNA' type='boolean' label='Search for tRNA genes'
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66 truevalue='-t' falsevalue='' checked="true" help='(-t)' />
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67 <param name='mtRNA' type='boolean' label='Search for Metazoan mitochondrial tRNA genes' truevalue='-mt' falsevalue='' checked="false"
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68 help='-i switch will be ignored. Composite Metazoan mitochondrial genetic code used. (-mt)' />
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69 <param name='mam_mtRNA' type='boolean' label='Search for Mammalian mitochondrial tRNA genes'
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70 truevalue='-mtmam' falsevalue='' checked="false" help='-i switch will be ignored. Mammalian mitochondrial genetic code used. (-mtmam)' />
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71 <param name='introns' type='boolean' label='Search for tRNA genes with introns in anticodon loop'
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72 truevalue='-i' falsevalue='' checked="false" help='With a maximum length of 3000 bases. (-i).' />
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73 <param name='secondary_structure' type='boolean' label='Print out secondary structure'
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74 truevalue='-fasta' falsevalue='-fon' checked="false" help='(-fasta,-fon)' />
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75 <param name="gff3_output" type='boolean' label='Convert output to GFF3' truevalue='True' falsevalue='' checked="false" help='' />
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76 <param name="gff3_model" type='boolean' label='Full gene model for GFF3 data' truevalue='--full' checked='false' help='' />
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77 </inputs>
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78 <outputs>
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79 <data name="output" format="fasta">
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80 <change_format>
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81 <when input="secondary_structure" value="-fasta" format="txt"/>
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82 </change_format>
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83 </data>
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84 <data format="gff3" name="gff3_output_file" >
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85 <filter>gff3_output</filter>
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86 </data>
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87 </outputs>
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88 <tests>
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89 <test>
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90 <param name="input" value="trna_arabidopsis.fasta" ftype="fasta" />
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91 <param name="genbank_gencode" value="1" />
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92 <param name="topology" value="-c" />
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93 <param name="tmRNA" value="True" />
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94 <param name="tRNA" value="True" />
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95 <param name="mtRNA" value="False" />
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96 <param name="mam_mtRNA" value="False" />
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97 <param name="introns" value="False" />
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98 <param name="secondary_structure" value="-fon" />
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99 <param name="gff3_output" value="false" />
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100 <output name="output" file="aragorn_tansl-table-1_tmRNA_tRNA.fasta" ftype="fasta" />
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101 </test>
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102
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103 <test>
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104 <param name="input" value="trna_arabidopsis.fasta" ftype="fasta" />
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105 <param name="genbank_gencode" value="1" />
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106 <param name="topology" value="-c" />
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107 <param name="tmRNA" value="True" />
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108 <param name="tRNA" value="True" />
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109 <param name="mtRNA" value="False" />
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110 <param name="mam_mtRNA" value="False" />
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111 <param name="introns" value="False" />
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112 <param name="secondary_structure" value="-fasta" />
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113 <param name="gff3_output" value="false" />
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114 <output name="output" file="aragorn_tansl-table-1_tmRNA_tRNA.txt" ftype="txt" lines_diff="2" />
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115 </test>
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116 <test>
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117 <param name="input" value="trna_arabidopsis.fasta" ftype="fasta" />
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118 <param name="genbank_gencode" value="1" />
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119 <param name="topology" value="-c" />
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120 <param name="tmRNA" value="True" />
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121 <param name="tRNA" value="True" />
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122 <param name="mtRNA" value="False" />
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123 <param name="mam_mtRNA" value="False" />
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124 <param name="introns" value="False" />
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125 <param name="gff3_output" value="True" />
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126 <output name="gff3_output_file" file="aragorn_tansl-table-1_tmRNA_tRNA.gff3" ftype="gff3" />
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127 </test>
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128 <test>
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129 <param name="input" value="genome_with_introns.fa" ftype="fasta" />
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130 <param name="genbank_gencode" value="11" />
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131 <param name="topology" value="-c" />
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132 <param name="tmRNA" value="True" />
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133 <param name="tRNA" value="True" />
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134 <param name="mtRNA" value="False" />
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135 <param name="mam_mtRNA" value="False" />
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136 <param name="introns" value="True" />
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137 <param name="gff3_output" value="True" />
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138 <param name="gff3_model" value="True" />
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139 <output name="gff3_output_file" file="aragorn_tansl-table-11_introns.gff3" ftype="gff3" />
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140 </test>
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141 </tests>
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142 <help>
1
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143 <![CDATA[
0
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144
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145 **What it does**
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146
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147 Aragorn_ predicts tRNA (and tmRNA) in nucleotide sequences.
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148
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149 .. _Aragorn: http://mbio-serv2.mbioekol.lu.se/ARAGORN/
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150
2
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151 **Input**
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152
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153 As input a genome sequence FASTA file is needed. Select the right genetic code and the topology for your organism and choose what you want to have analyzed.
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154
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155 By default, ARAGORN assumes that each sequence has a circular topology (search wraps around ends), that both strands should be searched, that the progress of the search is not reported, both tRNA and tmRNA genes are detected, and tRNA genes containing C‐loop introns are not detected.
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156
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157 **Output**
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158
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159 The output of Aragorn reports the proposed tRNA secondary structure and, for tmRNA genes, the secondary structure of the tRNA domain, the tmRNA gene sequence, the tag peptide and a list of organisms with matching tmRNA peptide tags.
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160
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161 Optionally, your output can be converted to GFF3.
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162
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163 **Example**
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164
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165 Suppose you have the following nucleotide sequences::
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166
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167 >SQ Sequence 8667507 BP; 1203558 A; 3121252 C; 3129638 G; 1213059 T; 0 other;
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168 cccgcggagcgggtaccacatcgctgcgcgatgtgcgagcgaacacccgggctgcgcccg
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169 ggtgttgcgctcccgctccgcgggagcgctggcgggacgctgcgcgtcccgctcaccaag
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170 cccgcttcgcgggcttggtgacgctccgtccgctgcgcttccggagttgcggggcttcgc
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171 cccgctaaccctgggcctcgcttcgctccgccttgggcctgcggcgggtccgctgcgctc
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172 ccccgcctcaagggcccttccggctgcgcctccaggacccaaccgcttgcgcgggcctgg
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173 ....
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174
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175 Running this tool can produce a FASTA file with all detected RNAs or a more detailed text file like the following::
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176
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177 c
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178 c
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179 a
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180 g-c
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181 g-c
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182 g-c
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183 c-g
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184 g-c
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185 a-t
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186 t-a ca
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187 t tgacc a
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188 ga a !!!!! g
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189 t ctcg actgg c
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190 g !!!! c tt
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191 g gagc t
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192 aa g g
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193 c-gag
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194 t-a
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195 t-a
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196 c-g
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197 g-c
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198 t c
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199 t a
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200 cac
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201
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202 tRNA-Val(cac)
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203 74 bases, %GC = 58.1
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204 Sequence [6669703,6669776]
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205
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206
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207 tRNA Anticodon Frequency
1
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208 AAA Phe GAA Phe 1 CAA Leu 1 TAA Leu 1
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209 AGA Ser GGA Ser 1 CGA Ser 2 TGA Ser 1
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210 ACA Cys GCA Cys 2 CCA Trp 2 TCA seC
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211 ATA Tyr GTA Tyr 1 CTA Pyl TTA Stop
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212 AAG Leu GAG Leu 3 CAG Leu 1 TAG Leu 2
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213 AGG Pro GGG Pro 2 CGG Pro 2 TGG Pro 2
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214 ACG Arg 1 GCG Arg 2 CCG Arg 1 TCG Arg
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215 ATG His GTG His 2 CTG Gln 2 TTG Gln 1
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216 AAC Val GAC Val 3 CAC Val 2 TAC Val 1
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217 AGC Ala GGC Ala 2 CGC Ala 3 TGC Ala 1
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218 ACC Gly GCC Gly 5 CCC Gly 1 TCC Gly 2
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219 ATC Asp GTC Asp 3 CTC Glu 2 TTC Glu 2
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220 AAT Ile GAT Ile 3 CAT Met 6 TAT Ile
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221 AGT Thr GGT Thr 2 CGT Thr 1 TGT Thr 2
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222 ACT Ser GCT Ser 1 CCT Arg 1 TCT Arg 1
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223 ATT Asn GTT Asn 3 CTT Lys 3 TTT Lys 2
0
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224 Ambiguous: 1
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225
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226 tRNA Codon Frequency
1
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227 TTT Phe TTC Phe 1 TTG Leu 1 TTA Leu 1
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228 TCT Ser TCC Ser 1 TCG Ser 2 TCA Ser 1
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229 TGT Cys TGC Cys 2 TGG Trp 2 TGA seC
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230 TAT Tyr TAC Tyr 1 TAG Pyl TAA Stop
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231 CTT Leu CTC Leu 3 CTG Leu 1 CTA Leu 2
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232 CCT Pro CCC Pro 2 CCG Pro 2 CCA Pro 2
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233 CGT Arg 1 CGC Arg 2 CGG Arg 1 CGA Arg
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234 CAT His CAC His 2 CAG Gln 2 CAA Gln 1
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235 GTT Val GTC Val 3 GTG Val 2 GTA Val 1
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236 GCT Ala GCC Ala 2 GCG Ala 3 GCA Ala 1
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237 GGT Gly GGC Gly 5 GGG Gly 1 GGA Gly 2
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238 GAT Asp GAC Asp 3 GAG Glu 2 GAA Glu 2
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239 ATT Ile ATC Ile 3 ATG Met 6 ATA Ile
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240 ACT Thr ACC Thr 2 ACG Thr 1 ACA Thr 2
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241 AGT Ser AGC Ser 1 AGG Arg 1 AGA Arg 1
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242 AAT Asn AAC Asn 3 AAG Lys 3 AAA Lys 2
0
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243 Ambiguous: 1
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244
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245 Number of tRNA genes = 86
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246 tRNA GC range = 50.0% to 85.1%
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247 Number of tmRNA genes = 1
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248
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249
1
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250 ]]>
0
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251 </help>
2
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252 <citations>
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253 <citation type="doi">10.1093/nar/gkh152</citation>
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254 </citations>
0
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255 </tool>