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1 <tool id="preprocess" name="preProcess" veision="1.0.0">
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2 <description>tool for Raw data preprocess analisis, including 3' adapter triming, reads collaping, genome mapping and rfam non-miRNA analysis </description>
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3
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4 <requirements>
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5 <requirement type="package" version="0.0.13">fastx_toolkit </requirement>
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6 <requirement type="package" version="0.12.7">bowtie</requirement>
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7 <requirement type="set_environment">SCRIPT_PATH</requirement>
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8 <!--requirement type="package" version="3.0.1">R</requirement!-->
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9 <requirement type="package" version="2.59">SVG</requirement>
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10 <requirement type="package" version="2.1.8">ViennaRNA</requirement>
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11 </requirements>
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12
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13 <!--command interpreter="perl">miPlant.pl -i $input -format $format -gfa $gfa -idx $index -pre $pre -mat $mat -rfam $rfam -idx2 $idx2 -D $D -a $a -M $M -min $min -max $max -mis $mis -e $e -f $f -v $v -r $r -dis $dis -flank $flank -mfe $mfe -t $t -o $output</command-->
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14
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50
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15 <command interpreter="perl">preProcess.pl
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16 ## Change this to accommodate the number of threads you have available.
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17 -t \${GALAXY_SLOTS:-4}
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18 -path \$SCRIPT_PATH
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19
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20 #for $j, $s in enumerate( $series )
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21 ##rank_of_series=$j
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22 -i ${s.input}
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23 -tag ${s.tag}
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24 #end for
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25
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26 ## Do or not annotate rfam non-miRNA RNAs
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27 #if $params.annotate_rfam == "yes":
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50
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28 ## prepare Rfam bowtie index
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29 #set rfam_index_path = ''
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30 #if str($params.annotate_rfam.reference_rfam.source) == "history":
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31 bowtie-build "$params.annotate_rfam.reference_rfam.own_file" rfam; ln -s "$params.annotate_rfam.reference_rfam.own_file" rfam.fa;
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32 #set rfam_index_path = 'rfam'
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33 #else:
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34 #set rfam_index_path = $params.annotate_rfam.reference_rfam.index.fields.path
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35 #end if
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36
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37 -rfam ${rfam_index_path}.fa -idx2 $rfam_index_path -v $v
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47
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38 #end if
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39
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40 ## prepare bowtie index
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41 #set index_path = ''
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42 #if str($reference_genome.source) == "history":
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43 bowtie-build "$reference_genome.own_file" genome; ln -s "$reference_genome.own_file" genome.fa;
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44 #set index_path = 'genome'
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45 #else:
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46 #set index_path = $reference_genome.index.fields.path
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47 #end if
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48
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50
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49 -format $format -gfa ${index_path}.fa -idx $index_path -phred $phred -a $a -M $mapnt -min $min -max $max -mis $mismatch > run.log
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47
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50 </command>
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51
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52 <inputs>
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53
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54 <repeat name="series" title="Series">
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55 <param name="input" type="data" label="Raw data"/>
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56 <param name="tag" type="text" data_ref="input" label="Sample name of raw data"/>
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57 </repeat>
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58
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59 <!--param name="input" format="tabular" type="data" label="input config file" /-->
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60
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61 <param name="format" type="select" lable="raw data format" multiple="false">
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62 <option value="fastq">Raw data is fastq. format</option>
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63 <option value="fasta">Raw data is fasta. format</option>
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64 </param>
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65
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66 <!-- reference genome -->
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67 <conditional name="reference_genome">
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68 <param name="source" type="select" label="Will you select a reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options">
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69 <option value="indexed">Use a built-in index</option>
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70 <option value="history">Use one from the history</option>
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71 </param>
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72 <when value="indexed">
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73 <param name="index" type="select" label="Select a reference genome" help="If your genome of interest is not listed, contact the Galaxy team">
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74 <options from_data_table="bowtie_indexes">
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75 <filter type="sort_by" column="2"/>
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76 <validator type="no_options" message="No indexes are available for the selected input dataset"/>
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77 </options>
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78 </param>
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79 </when>
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80 <when value="history">
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81 <param name="own_file" type="data" format="fasta" metadata_name="dbkey" label="Select the reference genome" />
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82 </when>
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83 </conditional>
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84
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85 <!--param name="gfa" type="data" label="genome sequence fasta file"/-->
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86 <!--param type="data" name="index" label="genome sequence bowtie index"/-->
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50
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87 <param name="phred" type="select" lable="input quals are Phred+64 or Phred+33" multiple="false">
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88 <option value="64">Phred+64</option>
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89 <option value="33" selected="true">Phred+33</option>
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90 </param>
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91 <param name="a" type="text" value="ATCTCGTATG" label="3' adapter sequence" />
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92 <param name="mapnt" type="integer" value="8" label="minimum adapter map nts" />
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93 <param name="min" type="integer" value="19" label="minimum microRNA length" />
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94 <param name="max" type="integer" value="28" label="maximum microRNA length" />
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95 <param name="mismatch" type="integer" value="0" label="number of allowed mismatches when mapping reads to genome" />
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96
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97 <conditional name="params">
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98 <param name="annotate_rfam" type="select" label="annotate rfam nocoding RNAs(excluding miRNA)">
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99 <option value="yes" selected="true">yes</option>
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100 <option value="no">no</option>
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101 </param>
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102 <when value="yes">
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50
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103 <!--param name="rfam" type="data" label="rfam sequence file" /-->
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104 <conditional name="reference_rfam">
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105 <param name="source" type="select" label="Will you select a reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options">
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106 <option value="indexed">Use a built-in index</option>
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107 <option value="history">Use one from the history</option>
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108 </param>
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109 <when value="indexed">
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110 <param name="index" type="select" label="Select a reference" help="If your reference of interest is not listed, contact the Galaxy team">
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111 <options from_data_table="rfam_bowtie_indexes">
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112 <filter type="sort_by" column="2"/>
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113 <validator type="no_options" message="No indexes are available for the selected input dataset"/>
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114 </options>
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115 </param>
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116 </when>
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117 <when value="history">
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118 <param name="own_file" type="data" format="fasta" metadata_name="dbkey" label="Select the reference" />
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119 </when>
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120 </conditional>
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121
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122 <param name="v" type="integer" value="0" label="report end-to-end hits less than v mismatches for rfam mapping"/>
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123 </when>
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52
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124 </conditional>
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125
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126
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47
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127
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128 </inputs>
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129
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130 <outputs>
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131 <data format="html" name="preprocess result" from_work_dir="preProcess/preprocessResult.html" label="${tool.name} on ${on_string}: preprocess result"/>
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132
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133 <data format="txt" name="clean FASTA data" from_work_dir="preProcess/preProcess_clean/collapse_reads_$min_$max.fa" label="${tool.name} on ${on_string}: clean FASTA data"/>
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134
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135 <data format="txt" name="genome mapping result" from_work_dir="preProcess/genome_match/genome_mapped.bwt" label="${tool.name} on ${on_string}: genome mapping result"/>
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136 <data format="txt" name="genome mapped FASTA reads" from_work_dir="preProcess/genome_match/genome_mapped.fa" label="${tool.name} on ${on_string}: genome mapped FASTA reads"/>
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137
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138 <data format="txt" name="Rfam mapping result" from_work_dir="preProcess/rfam_match/rfam_mapped.bwt" label="${tool.name} on ${on_string}: Rfam mapping result">
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139 <filter>(params['annotate_rfam'] == 'Yes')</filter>
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140 </data>
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141 <data format="txt" name="Rfam mapped FASTA file" from_work_dir="preProcess/rfam_match/rfam_mapped.fa" label="${tool.name} on ${on_string}: Rfam mapped FASTA file">
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142 <filter>(params['annotate_rfam'] == 'Yes')</filter>
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143 </data>
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144 <data format="txt" name="Rfam not mapped FASTA file" from_work_dir="preProcess/rfam_match/rfam_not_mapped.fa" label="${tool.name} on ${on_string}: Rfam not mapped FASTA file">
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145 <filter>(params['annotate_rfam'] == 'Yes')</filter>
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146 </data>
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147 <data format="html" name="input config" from_work_dir="preProcess/input_config" label="${tool.name} on ${on_string}: input config"/>
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148
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47
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149 </outputs>
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150
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151 <help>
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152
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153 </help>
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154 </tool>
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