view bam_to_bigwig/README.txt @ 1:0ff100a057ef

Migrated tool version 0.0.2 from old tool shed archive to new tool shed repository
author brad-chapman
date Tue, 07 Jun 2011 16:26:46 -0400
parents d2c1af657010
children e2edfa478eb4
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Convert a BAM file into a BigWig coverage file. This can be used directly from 
Galaxy for display at UCSC. The advantage over standard Wiggle format is that 
the data is stored in a compressed format and can be retrieved by genome
region. This allows you to view regions of arbitrarily large Wiggle file data
at UCSC while avoiding the upload costs.

The latest version of the bam_to_wiggle.py script is available at:

https://github.com/chapmanb/bcbb/blob/master/nextgen/scripts/bam_to_wiggle.py

Place the script in the same directory as the XML configuration file, or
provide a soft link to it.

This requires:

Python2, version 2.6 or better
pysam (http://code.google.com/p/pysam/)
wigToBigWig from UCSC (http://hgdownload.cse.ucsc.edu/admin/exe/)