annotate wgmlst_add.xml @ 1:5b2c48fa0175 draft

planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
author bvalot
date Wed, 07 Dec 2022 15:25:06 +0000
parents a3cc35af3635
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
1
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1 <tool id="wgmlst_add_wrapper" name="Add strain to cg/wgMLST database"
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2 version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="20.05">
0
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3 <description></description>
1
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4 <macros>
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5 <import>macro.xml</import>
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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6 </macros>
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7 <expand macro="requirements" />
0
a3cc35af3635 planemo upload for repository https://github.com/bvalot/pyMLST commit 13edfab02a5da9e374c38ecbd0e229ec0f8d53bb
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8 <stdio>
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9 <exit_code range="1:" level="fatal" />
a3cc35af3635 planemo upload for repository https://github.com/bvalot/pyMLST commit 13edfab02a5da9e374c38ecbd0e229ec0f8d53bb
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10 </stdio>
a3cc35af3635 planemo upload for repository https://github.com/bvalot/pyMLST commit 13edfab02a5da9e374c38ecbd0e229ec0f8d53bb
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11 <version_command>wgMLST -v</version_command>
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12 <command><![CDATA[
1
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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13 cp '${database}' '${databaseout}'
0
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14 #if $data.input == "fasta"
1
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15 #for $datain in $data.fastain:
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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16 && wgMLST add
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17 -s '$datain.name.replace(" ", "_").replace("-", "_")'
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18 #if str($coverage)
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19 -c $coverage
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20 #end if
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21 #if str($identity)
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22 -i $identity
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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23 #end if
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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24 '${databaseout}' '${datain}'
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25 #if $log
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26 2>> '${logfile}'
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27 #end if
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28 #end for
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29 #else if $data.input == "fastqsingle"
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30 #for $datain in $data.single:
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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31 && wgMLST add2
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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32 -s '$datain.name.replace(" ", "_").replace("-", "_")'
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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33 #if str($data.reads)
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34 -r $data.reads
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35 #end if
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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36 #if str($coverage)
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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37 -c $coverage
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38 #end if
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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39 #if str($identity)
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40 -i $identity
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41 #end if
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42 '${databaseout}' '${datain}'
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43 #if $log
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44 2>> '${logfile}'
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45 #end if
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46 #end for
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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47 #else if $data.input == "fastqpaired"
5b2c48fa0175 planemo upload for repository https://github.com/bvalot/pyMLST commit fe04f6232a5f7ea78b666cb8036872902e79998b
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48 && wgMLST add2
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49 -s '$data.pairedfile.name.replace(" ", "_").replace("-", "_")'
0
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50 #if str($data.reads)
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51 -r $data.reads
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52 #end if
1
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53 #if str($coverage)
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54 -c $coverage
0
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55 #end if
1
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56 #if str($identity)
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57 -i $identity
0
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58 #end if
1
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59 '${databaseout}' '${data.pairedfile.forward}' '${data.pairedfile.reverse}'
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60 #if $log
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61 2>> '${logfile}'
0
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62 #end if
1
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63 #else
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64 #for $datain in $data.pairedfilelist:
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65 && wgMLST add2
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66 -s '$datain.name.replace(" ", "_").replace("-", "_")'
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67 #if str($data.reads)
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68 -r $data.reads
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69 #end if
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70 #if str($coverage)
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71 -c $coverage
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72 #end if
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73 #if str($identity)
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74 -i $identity
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75 #end if
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76 '${databaseout}' '${datain.forward}' '${datain.reverse}'
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77 #if $log
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78 2>> '${logfile}'
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79 #end if
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80 #end for
0
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81 #end if
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82 ]]>
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83 </command>
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84 <inputs>
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85 <param name="database" type="data"
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86 format="sqlite"
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87 label="cg/wgMLST database"
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88 help="Sqlite format from cg/wgMLST database" />
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89 <conditional name="data">
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90 <param name="input" type="select" label="Select type of data">
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91 <option value="fasta" selected="true">Assembly (fasta)</option>
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92 <option value="fastqsingle">Raw data (single)</option>
1
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93 <option value="fastqpaired">Raw data (paired)</option>
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94 <option value="fastqpaired2">Raw data (List of paired)</option>
0
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95 </param>
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96 <when value="fasta">
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97 <param name="fastain" type="data" format="fasta"
1
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98 label="Assembly Genome" help="Fasta format"
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99 multiple="true" />
0
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100 </when>
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101 <when value="fastqsingle">
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102 <param name="single" type="data" format="fastq,fastq.gz"
1
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103 label="Single read file" help="Fastq(gz) format"
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104 multiple="true" />
0
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105 <param name="reads" type="integer" value="10" optional="true"
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106 label="Minimum reads coverage to search gene" />
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107 </when>
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108 <when value="fastqpaired">
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109 <param name="pairedfile" type="data_collection" format="fastq,fastq.gz"
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110 label="Paired of read files" help="Fastq(gz) format"
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111 collection_type="paired" />
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112 <param name="reads" type="integer" value="10" optional="true"
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113 label="Minimum reads coverage to search gene" />
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114 </when>
1
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115 <when value="fastqpaired2">
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116 <param name="pairedfilelist" type="data_collection" format="fastq,fastq.gz"
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117 label="Paired of read files" help="Fastq(gz) format"
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118 collection_type="list:paired" />
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119 <param name="reads" type="integer" value="10" optional="true"
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120 label="Minimum reads coverage to search gene" />
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121 </when>
0
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122 </conditional>
1
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123 <!-- <param name="strain" type="text" value="" size="50" -->
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124 <!-- optional="false" -->
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125 <!-- label="Strain" -->
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126 <!-- help="Name of the strain"> -->
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127 <!-- <sanitizer invalid_char="_" > -->
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128 <!-- <valid initial="string.ascii_letters,string.digits"> -->
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129 <!-- <add value="_" /> -->
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130 <!-- <add value="." /> -->
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131 <!-- </valid> -->
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132 <!-- </sanitizer> -->
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133 <!-- </param> -->
0
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134 <param name="identity" type="float" value="0.9" optional="false"
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135 label="Identity"
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136 help="Minimum identity to search gene" />
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137 <param name="coverage" type="float" value="0.9" optional="false"
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138 label="Coverage"
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139 help="Minimum coverage to search gene" />
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140 <param name="log" type="boolean" checked="true"
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141 label="Write log file" />
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142 </inputs>
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143 <outputs>
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144 <data name="databaseout" format="sqlite" label="${database.name}">
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145 </data>
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146 <data name="logfile" format="txt" label="${tool.name} on ${on_string}: log">
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147 <filter>log</filter>
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148 </data>
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149 </outputs>
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150 <tests>
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151 <test expect_num_outputs="2">
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152 <param name="database" value="wgmlst_ecoli.db" />
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153 <conditional name="data">
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154 <param name="input" value="fasta" />
1
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155 <param name="fastain" ftype="fasta" value="EHSB-021.fasta,EHSB-092.fasta" />
0
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156 </conditional>
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157 <param name="identity" value="0.8" />
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158 <output name="logfile" ftype="txt">
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159 <assert_contents>
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160 <has_text text="Added 2497 new MLST genes to the database" />
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161 <has_text text="Found 32 partial genes, filled 30" />
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162 <has_text text="Removed 4 genes" />
1
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163 <has_text text="Added 2496 new MLST genes to the database" />
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164 <has_text text="Found 44 partial genes, filled 40" />
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165 <has_text text="Removed 5 genes" />
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166 </assert_contents>
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167 </output>
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168 </test>
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169 <test expect_num_outputs="2">
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170 <param name="database" value="wgmlst_ecoli.db" />
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171 <conditional name="data">
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172 <param name="input" value="fastqsingle" />
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173 <param name="single"
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174 value="EHSB-021_R1.fastq.gz,EHSB-021_R2.fastq.gz" />
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175 <param name="reads" value="5" />
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176 </conditional>
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177 <output name="logfile" ftype="txt">
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178 <assert_contents>
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179 <has_text_matching expression="Add 14\d\d new MLST genes to database" />
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180 <has_text_matching expression="Remove \d genes with uncertain bases" />
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181 <has_text_matching expression="Remove \d\d\d genes with bad CDS" />
0
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182 </assert_contents>
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183 </output>
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184 </test>
1
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185 <test expect_num_outputs="2">
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186 <param name="database" value="wgmlst_ecoli.db" />
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187 <conditional name="data">
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188 <param name="input" value="fastqpaired" />
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189 <param name="pairedfile">
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190 <collection type="paired">
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191 <element name="forward" value="EHSB-021_R1.fastq.gz" />
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192 <element name="reverse" value="EHSB-021_R2.fastq.gz" />
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193 </collection>
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194 </param>
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195 <param name="reads" value="5" />
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196 </conditional>
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197 <output name="logfile" ftype="txt">
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198 <assert_contents>
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199 <has_text_matching expression="Add 24\d\d new MLST genes to database" />
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200 <has_text_matching expression="Remove \d genes with uncertain bases" />
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201 <has_text_matching expression="Remove \d\d genes with bad CDS" />
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202 </assert_contents>
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203 </output>
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204 </test>
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205 <test expect_num_outputs="2">
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206 <param name="database" value="wgmlst_ecoli.db" />
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207 <conditional name="data">
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208 <param name="input" value="fastqpaired2" />
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209 <param name="pairedfilelist">
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210 <collection type="list:paired">
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211 <element name="EHSB-021">
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212 <collection type="paired">
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213 <element name="forward" value="EHSB-021_R1.fastq.gz" />
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214 <element name="reverse" value="EHSB-021_R2.fastq.gz" />
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215 </collection>
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216 </element>
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217 <element name="EHSB-021bis">
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218 <collection type="paired">
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219 <element name="forward" value="EHSB-021_R1.fastq.gz" />
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220 <element name="reverse" value="EHSB-021_R2.fastq.gz" />
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221 </collection>
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222 </element>
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223 </collection>
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224 </param>
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225 <param name="reads" value="5" />
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226 </conditional>
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227 <output name="logfile" ftype="txt">
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228 <assert_contents>
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229 <has_text_matching expression="Add 24\d\d new MLST genes to database" />
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230 <has_text_matching expression="Remove \d genes with uncertain bases" />
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231 <has_text_matching expression="Remove \d\d genes with bad CDS" />
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232 </assert_contents>
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233 </output>
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234 </test>
0
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235 </tests>
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236 <help>
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237 **What it does**
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238
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239 Add a Strain to the cg/wgMLST database
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240
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241 You can use
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242 - Genome assembly (blat search)
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243 - Raw data (kma search)
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244
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245 **Options:**
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246 -s, --strain TEXT Name of the strain (default:genome name).
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247 -i, --identity FLOAT Minimum identity to search gene (default=0.9).
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248 -c, --coverage FLOAT Minimum coverage to search gene (default=0.95).
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249 -r, --reads INTEGER Minimum reads coverage to search gene (default=10).
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250 -f, --fasta FILENAME Write fasta file with gene allele.
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251
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252 **License and citation**
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253
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254 This Galaxy tool is Copyright © 2022 `B. Valot` and is released under the `GPL3 license`.
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255 </help>
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256 <citations>
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257 </citations>
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258 </tool>