annotate mirnature.xml @ 0:4394f98e705e draft default tip

"planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
author cavelandiah
date Sun, 27 Nov 2022 22:00:05 +0000
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4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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1 <tool id="mirnature" name="miRNAture" version="1.1+galaxy0" python_template_version="3.5" profile="21.05">
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2 <description>Computational detection of canonical microRNAs</description>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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3 <macros>
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4 <import>macros.xml</import>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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5 </macros>
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6 <expand macro="requirements" />
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7 <command detect_errors="aggressive"><![CDATA[
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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8 ##Last data path from: https://doi.org/10.5281/zenodo.7180160
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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9 ##MIRNATURE_DATA_PATH=\$(dirname \$(which miRNAture))/../share/blockclust_data &&
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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10
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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11 #import re
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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12 mkdir -p output &&
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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13 #if $blast_specific.blast_searches == 1:
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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14 mkdir -p $__tool_directory__/queries_to_test &&
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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15 cp '$queries_to_blast' $__tool_directory__/queries_to_test/Unknown_specie.fa &&
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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16 bash $__tool_directory__/generate_blast_folder.sh Unknown_specie.fa > queries_description.txt &&
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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17 mv queries_description.txt $__tool_directory__/queries_to_test/ &&
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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18 #end if
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19
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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20 bash $__tool_directory__/create_dataset.sh '$dataset' '$__tool_directory__' &&
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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21
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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22 miRNAture
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23 -stage '$stage'
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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24 #if $activate_subset.subset_models_des == 1:
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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25 -sublist '$subset_models'
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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26 #end if
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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27 -nbitscore_cut '$nbitscore'
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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28 -dataF $__tool_directory__/uncompress/Dataset/
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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29 -specie_genome '$specie_genome'
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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30 -specie_name '$specie_name'
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31 -specie_tag '$specie_tag'
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32 -pe '$parallel_linux_select'
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33 -workdir 'output/'
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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34 -m "${",".join(map(str, $homology_mode)) + ",final"}"
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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35 #if $blast_specific.blast_searches == 1:
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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36 #set $joined_str=",".join(map(str, $blast_specific.blast_strategy))
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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37 #set $complete_str=($joined_str + ",ALL")
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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38 -strategy '$complete_str'
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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39 -blastq '$__tool_directory__/queries_to_test/'
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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40 #end if
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41 -rep '$repeat_filter' > '$std_output' &&
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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42 rm -rf $__tool_directory__/uncompress/
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43 ]]></command>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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44
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45 <inputs>
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46 <!--File-->
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47 <param argument="-specie_genome" format="fasta" multiple="true" type="data"
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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48 label="Input genome or sequence"
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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49 help="Input sequence to be processed by miRNAture" />
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50 <!--Hidden-->
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51 <param argument="-pe" name="parallel_linux_select" type="integer" value="1" label="Parallel running" help="Test Parallel Linux" >
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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52 <validator type="in_range" message="Please set this option to 1." min="1" max="1"/>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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53 </param>
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54
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55 <!--TEXT-->
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56 <param argument="-specie_name" type="text" label="Scientific specie name as: Genera_specie" />
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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57 <param argument="-specie_tag" type="text" label="Species tag to identify through experiment" />
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58
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59 <!--NUM-->
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60 <param argument="-nbitscore_cut" name="nbitscore" type="float" label="nBitscore"
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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61 value="1" min="0" max="1" help="Control bitscore threshold to filter Rfam candidates" />
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62
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63 <param argument="-rep" name="repeat_filter" type="select" label="Repeats filter" help="Repetition cutoff" >
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64 <option value="relax,150,100">relax</option>
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65 <option value="default,200,100">default</option>
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66 </param>
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67
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68 <!--Binary-->
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69 <conditional name="activate_subset">
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70 <!--Binary-->
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71 <param argument="-sublist" name="subset_models_des" type="select" label="Subset default miRNA families" help="Make a selection of specific miRNA families to be search">
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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72 <option value="0">No</option>
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73 <option value="1">Yes</option>
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74 </param>
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75 <when value="0">
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76 </when>
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77 <when value="1">
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78 <param format="txt" multiple="true" name="subset_models" type="data"
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79 label="List of miRNA models to be searched"
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80 help="Input sequence to be processed by miRNAture" optional="true"/>
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81 </when>
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parents:
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82 </conditional>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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83
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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84 <param argument="-dataF" format="gz" multiple="true" name="dataset" type="data"
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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parents:
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85 label="Input Pre-calculated data"
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parents:
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86 help="Please submit the pre-calculated data to run miRNAture (see https://doi.org/10.5281/zenodo.7180160)" />
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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parents:
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87
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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parents:
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88 <!--Static list-->
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parents:
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89 <param argument="-stage" type="select" label="Stages to run">
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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90 <option value="complete">complete</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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91 <option value="homology">homology</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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92 <option value="no_homology">no_homology</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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93 <option value="validation">validation</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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94 <option value="evaluation">evaluation</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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95 <option value="summarise">summarise</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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96 </param>
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97 <!--Check Boxes-->
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98 <param argument="-mode" name="homology_mode" type="select" multiple="true" label="Homology mode">
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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99 <option value="blast">blast</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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100 <option value="rfam">rfam</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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101 <option value="mirbase">mirbase</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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102 <option value="hmm">hmm</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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103 </param>
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104
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105 <conditional name="blast_specific">
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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106 <param name="blast_searches" type="select" label="Use the blast mode to search annotated queries in your target genome?" help="Activate this option if wanted to use blast mode with provided sequences">
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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107 <option value="0">No</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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108 <option value="1">Yes</option>
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109 </param>
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110 <when value="0">
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111 </when>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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112 <when value="1">
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113 <param argument="-strategy" name="blast_strategy" type="select" multiple="true" label="Select one or more blast strategy(ies)">
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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114 <option value="1">1</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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115 <option value="2">2</option>
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116 <option value="3">3</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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117 <option value="4">4</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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118 <option value="5">5</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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119 <option value="6">6</option>
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120 <option value="7">7</option>
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121 <option value="8">8</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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122 <option value="9">9</option>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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123 </param>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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124 <param argument="-blstq" format="fasta" multiple="true" name="queries_to_blast" type="data"
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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125 label="Query sequences"
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126 help="Query sequences to search in target genome" />
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127 </when>
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128 </conditional>
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129 </inputs>
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130
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131 <outputs>
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132 <data name="std_output" format="txt" label="Standard output miRNAture" />
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133 <data format="txt" name="report0" label="Homology output miRNAture">
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134 <filter>stage == "homology" </filter>
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135 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.yaml" directory="output/" ext="yaml" visible="true"/>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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136 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.gff3" directory="output/miRNA_prediction/Final_Candidates" ext="gff3" visible="true" />
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137 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.txt\.db" directory="output/miRNA_prediction/Final_Candidates" visible="true" />
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138 </data>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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139 <data format="txt" name="report1" label="Complete output miRNAture">
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140 <filter>stage == "complete" </filter>
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141 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.yaml" directory="output/" ext="yaml" visible="true"/>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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142 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.gff3" directory="output/Final_miRNA_evaluation" ext="gff3" visible="true" />
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143 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.txt" directory="output/Final_miRNA_evaluation" ext="txt" visible="true" />
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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144 <discover_datasets pattern="(?P&lt;designation&gt;.+)\.fasta" directory="output/Final_miRNA_evaluation/Fasta" ext="fasta" visible="true" />
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145 </data>
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146 </outputs>
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147 <tests>
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148 <test>
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149 <param name="stage" value="homology"/>
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150 <conditional name="activate_subset">
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151 <param name="subset_models_des" value="1"/>
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152 <param name="subset_models" value="fam.txt"/>
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153 </conditional>
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154 <param name="nbitscore" value="1.0"/>
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155 <param name="dataset" value="Dataset_mirnature_tutorial.tar.gz"/>
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156 <param name="specie_genome" value="test.fasta"/>
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157 <param name="specie_name" value="Test_specie"/>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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158 <param name="specie_tag" value="Test"/>
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159 <param name="homology_mode" value="hmm"/>
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160 <param name="parallel_linux_select" value="1"/>
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161 <param name="repeat_filter" value="relax"/>
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162 <output name="std_output" file="test.txt"/>
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163 </test>
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164 </tests>
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165
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166 <help><![CDATA[
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167
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168 **miRNAture** detects *bona fide* miRNA candidates through sequence homology
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169 searches and validation steps using structural alignments with
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170 pre-defined or/and modified miRNA-specific covariance models. The
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171 miRNAture pipeline is composed of three modules: (1) Homology search
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172 operating on miRNA precursors, (2) prediction of the positioning of
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173 mature miRNAs within the precursor mature annotation, and (3) an
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174 Evaluation scheme designed to identify false positive miRNA annotations.
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175 This multi-stage approach generates annotation files in BED/GFF3 from
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176 precursors and detected mature regions and corresponding FASTA files. At
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177 the same time, a summary file with the MFE, precursor length and number
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178 of loci of each annotated miRNA family.
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179
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180 AUTHORS:
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181 *Cristian A. Velandia Huerto*, *Joerg Fallmann* and *Peter F. Stadler*
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182
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183 USAGE:
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184 ./miRNAture [-options]
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185
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186 OPTIONS:
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187 -h/-help Print this documentation.
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188
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189 -blstq/-blastQueriesFolder <PATH>
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190 Path of blast query sequences in FASTA format to be searched
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191 on the subject sequence.
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192
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193 -dataF/-datadir <PATH>
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194 Path to pre-calculated data directory containing RFAM and
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195 miRBase covariance, hidden markov models, and necessary
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196 files to run MIRfix.
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197
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198 -m/-mode <blast,hmm,rfam,mirbase>
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199 Homology search modes: blast, hmm, rfam, mirbase, and/or infernal.
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200 It is possible to perform individual analysis, but in this Galaxy
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201 version is always included the *final* option to merge multiple results.
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202
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203 -rep/-repetition_cutoff <relax,Number_Loci,Candidates_to_evaluate>
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204 Setup number of maximum loci number that will be evaluated
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205 by the mature's annotation stage. By default, miRNAture will
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206 detect miRNA families that report high number of loci (> 200
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207 loci). Then, it will select the top 100 candidates in terms
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208 of alignment scores, as candidates for the validation stage
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209 (default,200,100). The designed values could be modified by
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210 the following flag in the command line version:
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211 'relax,Number_Loci,Candidates_to_evaluate'. This option
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212 allows to the user to select the threshold values to detect
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213 repetitive families. The first parameter is <relax>, which
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214 tells miRNAture to change the default configuration. The
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215 next one, <Number_Loci> is the threshold of loci number to
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216 classify a family as repetitive. The last one,
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217 <Candidates_to_evaluate>, is the number of candidates prone
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218 to be evaluated in the next evaluation section. The rest
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219 candidates are included as homology 'potential' candidates.
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220 Selected option for this Galaxy version is set as:
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221 <relax,150,100>.
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222
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223 -str/-strategy <1,2,3,4,5,6,7,8,9,10>
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224 This flag is blast mode specific. It corresponds to blast
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225 strategies that would be used to search miRNAs. It must be
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226 indicated along with -m *Blast* flag.
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227
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228 -stg/-stage <'homology','no_homology','validation','evaluation','summarise','complete'>
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229 Selects the stage to be run on miRNAture. The options are:
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230 'homology', 'no_homology', 'validation', 'evaluation',
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231 'summarise' or 'complete'.
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232
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233 -speG/-specie_genome <PATH>
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234 Path of target sequences to be analyzed in FASTA format.
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235
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236 -speN/-specie_name <Genera_specie>
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237 Specie or sequence source's scientific name. The format must
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238 be: *Genera_specie*, separated by '_'.
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239
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240 -speT/-specie_tag <TAG_NAME>
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241 Experiment tag. Will help to identify the generated files
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242 along miRNA output files.
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243
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cavelandiah
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244 -sublist/-subset_models <FILE_WITH_CM_NAMES>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
cavelandiah
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245 Target list of CMs to be searched on subject
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
cavelandiah
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246 genome/sequences. If not indicated, miRNAture will run all
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
cavelandiah
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247 RFAM v14.4 metazoan miRNA models.
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
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248
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
cavelandiah
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249 -w/-workdir <OUT_PATH>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
cavelandiah
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250 Working directory path to write all miRNAture results.
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
cavelandiah
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251
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
cavelandiah
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252 BUGS, CAVEATS, COMPLAINS or DONATIONS
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
cavelandiah
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253 Write directly to cristian at bioinf.uni-leipzig.de
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
cavelandiah
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254
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cavelandiah
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255 ]]></help>
4394f98e705e "planemo upload for repository https://github.com/Bierinformatik/miRNAture/tree/galaxy_add/Galaxy/miRNAture commit 47a893683a9445abddce77c28f43d098b62cf385"
cavelandiah
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256 <expand macro="citations" />
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cavelandiah
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257 </tool>