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author | cpt |
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date | Mon, 05 Jun 2023 02:50:07 +0000 |
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#!/usr/bin/perl # # Code written by Eric Rasche # mailto:rasche.eric@yandex.ru # tel:404.692.2048 # http://eric.rasche.co.uk # for # Center for Phage Technology # use strict; use warnings; use CPT::GalaxyGetOpt; use Data::Dumper; my $ggo = CPT::GalaxyGetOpt->new(); my %colors = map { $_ => $_ } qw(red orange yellow green blue gray black white); my %intensity = map { $_ => $_ } qw (vvvvl vvvl vvl vl vd vvd vvvd vvvvd); my $options = $ggo->getOptions( 'options' => [ [ 'file', 'Input file', { required => 1, validate => 'File/Input', file_format => ['genbank', 'embl', 'txt'], } ], [ 'chromosome' => 'Name for the chromosome inside Circos', { required => 1, validate => 'String' } ], [ 'color' => 'Color to use for Circos plot', { required => 1, validate => 'Option', options => \%colors, } ], [ 'intensity' => 'Circos color intensity. ', { validate => 'Option', options => \%intensity, } ], ], 'outputs' => [ [ 'circosk', 'Circos Karyotype File', { validate => 'File/Output', required => 1, default => 'karyotype', data_format => 'text/plain', default_format => 'TXT' } ], ], 'defaults' => [ 'appname' => 'Genbank2CircosK', 'appid' => 'Genbank2CircosK', 'appvers' => '1.94', 'appdesc' => 'Convert genbank files to Circos Karyotype configuration files', ], 'tests' => [ { test_name => "Default", params => { 'file' => 'test-data/inputs/multi.gbk', 'chromosome' => 'test', 'color' => 'red', 'intensity' => 'vvvl', }, outputs => { 'circosk' => ['karyotype.txt', 'test-data/outputs/circosk.conf'], } }, ], ); use CPT::Bio; my $bio = CPT::Bio->new(); my @results; my $c = 0; my $seqio = $bio->requestCopyIO( file => $options->{file} ); while(my $seqobj = $seqio->next_seq()){ foreach my $feat ( $seqobj->get_SeqFeatures () ) { #band test 12 CDS__test_1gbk 5715 6335 red] next if ( $feat->primary_tag ne 'CDS' ); my $id = $bio->_getIdentifier($feat); $id =~ s/\s+/_/g; push( @results, join( ' ', ( 'band', $options->{'chromosome'}, $c++, $id, $feat->start, $feat->end, (defined $options->{'intensity'} ? $options->{'intensity'} : '') . $options->{'color'} ) ) ); } } my $z = join( "\n", @results ); use CPT::OutputFiles; my $output = CPT::OutputFiles->new( name => 'circosk', GGO => $ggo, ); $output->CRR(data => $z);