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comparison skesa.xml @ 14:1581b4e8d993 draft

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author estrain
date Thu, 16 Aug 2018 14:11:34 -0400
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13:a28a41742990 14:1581b4e8d993
1 <tool id="skesa" name="skesa" version="0.1">
2 <requirements>
3 <requirement type="package" version="2.2">skesa</requirement>
4 </requirements>
5 <command detect_errors="exit_code"><![CDATA[
6 #if $jobtype.select != "cl"
7 skesa
8 #if $jobtype.select == "srr"
9 -sra_run $srrnum
10 #else if $jobtype.select == "asm"
11 --fasta $draft
12 #else if $jobtype.select == "se"
13 --fastq $fastq1
14 #else if $jobtype.select == "pe"
15 --fastq $fastq1,$fastq2 --use_paired_ends
16 #else if $jobtype.select == "rp"
17 --fastq $pairedf.forward,$pairedf.reverse --use_paired_ends
18 #end if
19 #if $cores != 0
20 --cores $cores
21 #end if
22 --memory $memory > results.skesa.fasta
23 #end if
24
25 ]]></command>
26 <inputs>
27 <conditional name="jobtype">
28 <when value="srr">
29 <param name="srrnum" type="text" label="Sra run number"/>
30 </when>
31 <param name="select" type="select" label="Assembly or FASTQ Reads?">
32 <option value="sra">SRR number</option>
33 <option value="asm">Genome Assembly</option>
34 <option value="se">Single-End Reads</option>
35 <option value="pe">Paired-End Reads (Separate Files)</option>
36 <option value="rp">Paired-End Reads (Paired Data Set)</option>
37 <option value="cl">Collection of Reads</option>
38 </param>
39 <when value="asm">
40 <param name="draft" type="data" format="fasta" label="FASTA" />
41 </when>
42 <when value="se">
43 <param name="fastq1" type="data" format="fastq" label="FASTQ" />
44 </when>
45 <when value="pe">
46 <param name="fastq1" type="data" format="fastq" label="FASTQ" />
47 <param name="fastq2" type="data" format="fastq" label="FASTQ" />
48 </when>
49 <when value="cl">
50 <param type="data_collection" name="collection_files" format="fastq" collection_type="list" label="FASTQS: Must be a Data Set list built from multiple fastq files" />
51 </when>
52 <when value="rp">
53 <param type="data_collection" collection_type="paired" name="pairedf" format="fastq" label="FASTQS: Must be a paired set of forward and reverse fastq files" />
54 </when>
55 </conditional>
56 <param name="memory" type="integer" label="Memory available (GB) [integer]" value="16" />
57 <param name="cores" type="integer" label="Number of cores to use (default all) [integer]" value="0" />
58 <param name="kmer" type="integer" label="Minimal kmer length for assembly [default 21] if non are specified " value="0" />
59 <param name="min_count" type="integer" label="Minimal count for kmers retained for comparing alternate choices [integer]" value="0" />
60
61 </inputs>
62 <outputs>
63 <data format="fasta" label="skesa Results" name="${input.name}.skesa.fasta" from_work_dir="*.fasta"/>
64 </outputs>
65
66 <help><![CDATA[
67
68 **Usage: skesa**
69
70 **INPUT**
71
72 A fasta assembly or single or paired end reads test or data set list of fastqs
73
74 **Memory available**
75
76 --memory arg (=32) Memory available (GB) [integer]
77
78
79 **Number of cores**
80
81 --cores arg (=0) Number of cores to use (default all) [integer]
82
83 https://github.com/ncbi/ngs-tools/tree/master/tools/skesa/
84
85 ]]></help>
86 <citations>
87 <citation type="bibtex">
88 @misc{pope_dashnow_zobel_holt_raven_schultz_inouye_tomita_2014,
89 title={skesa: eSKESA is a de-novo sequence read assembler for cultured single isolate genomes
90 based on DeBruijn graphs. It uses conservative heuristics and is designed to
91 create breaks at repeat regions in the genome. This leads to excellent sequence
92 quality but not necessarily a large N50 statistic. It is a multi-threaded
93 application that scales well with the number of processors. For different runs
94 with the same inputs, including the order of reads, the order and orientation
95 of contigs in the output is deterministic. },
96 url={https://github.com/ncbi/ngs-tools/tree/master/tools/skesa/},
97 author={National Center for Biotechnology Information },
98 }</citation>
99 </citations>
100 </tool>