Mercurial > repos > cstrittmatter > skesa
changeset 14:1581b4e8d993 draft
Uploaded
author | estrain |
---|---|
date | Thu, 16 Aug 2018 14:11:34 -0400 |
parents | a28a41742990 |
children | 06d5e64a0586 |
files | skesa.xml |
diffstat | 1 files changed, 100 insertions(+), 0 deletions(-) [+] |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/skesa.xml Thu Aug 16 14:11:34 2018 -0400 @@ -0,0 +1,100 @@ +<tool id="skesa" name="skesa" version="0.1"> + <requirements> + <requirement type="package" version="2.2">skesa</requirement> + </requirements> + <command detect_errors="exit_code"><![CDATA[ + #if $jobtype.select != "cl" + skesa + #if $jobtype.select == "srr" + -sra_run $srrnum + #else if $jobtype.select == "asm" + --fasta $draft + #else if $jobtype.select == "se" + --fastq $fastq1 + #else if $jobtype.select == "pe" + --fastq $fastq1,$fastq2 --use_paired_ends + #else if $jobtype.select == "rp" + --fastq $pairedf.forward,$pairedf.reverse --use_paired_ends + #end if + #if $cores != 0 + --cores $cores + #end if + --memory $memory > results.skesa.fasta + #end if + + ]]></command> + <inputs> + <conditional name="jobtype"> + <when value="srr"> + <param name="srrnum" type="text" label="Sra run number"/> + </when> + <param name="select" type="select" label="Assembly or FASTQ Reads?"> + <option value="sra">SRR number</option> + <option value="asm">Genome Assembly</option> + <option value="se">Single-End Reads</option> + <option value="pe">Paired-End Reads (Separate Files)</option> + <option value="rp">Paired-End Reads (Paired Data Set)</option> + <option value="cl">Collection of Reads</option> + </param> + <when value="asm"> + <param name="draft" type="data" format="fasta" label="FASTA" /> + </when> + <when value="se"> + <param name="fastq1" type="data" format="fastq" label="FASTQ" /> + </when> + <when value="pe"> + <param name="fastq1" type="data" format="fastq" label="FASTQ" /> + <param name="fastq2" type="data" format="fastq" label="FASTQ" /> + </when> + <when value="cl"> + <param type="data_collection" name="collection_files" format="fastq" collection_type="list" label="FASTQS: Must be a Data Set list built from multiple fastq files" /> + </when> + <when value="rp"> + <param type="data_collection" collection_type="paired" name="pairedf" format="fastq" label="FASTQS: Must be a paired set of forward and reverse fastq files" /> + </when> + </conditional> + <param name="memory" type="integer" label="Memory available (GB) [integer]" value="16" /> + <param name="cores" type="integer" label="Number of cores to use (default all) [integer]" value="0" /> + <param name="kmer" type="integer" label="Minimal kmer length for assembly [default 21] if non are specified " value="0" /> + <param name="min_count" type="integer" label="Minimal count for kmers retained for comparing alternate choices [integer]" value="0" /> + + </inputs> + <outputs> + <data format="fasta" label="skesa Results" name="${input.name}.skesa.fasta" from_work_dir="*.fasta"/> + </outputs> + + <help><![CDATA[ + +**Usage: skesa** + +**INPUT** + +A fasta assembly or single or paired end reads test or data set list of fastqs + +**Memory available** + +--memory arg (=32) Memory available (GB) [integer] + + +**Number of cores** + +--cores arg (=0) Number of cores to use (default all) [integer] + +https://github.com/ncbi/ngs-tools/tree/master/tools/skesa/ + + ]]></help> + <citations> + <citation type="bibtex"> + @misc{pope_dashnow_zobel_holt_raven_schultz_inouye_tomita_2014, + title={skesa: eSKESA is a de-novo sequence read assembler for cultured single isolate genomes + based on DeBruijn graphs. It uses conservative heuristics and is designed to + create breaks at repeat regions in the genome. This leads to excellent sequence + quality but not necessarily a large N50 statistic. It is a multi-threaded + application that scales well with the number of processors. For different runs + with the same inputs, including the order of reads, the order and orientation + of contigs in the output is deterministic. }, + url={https://github.com/ncbi/ngs-tools/tree/master/tools/skesa/}, + author={National Center for Biotechnology Information }, + }</citation> + </citations> +</tool>