Mercurial > repos > davidvanzessen > argalaxy_tools
annotate README.md @ 54:81b3eb11ed2c draft
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author | rhpvorderman |
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date | Tue, 16 Nov 2021 15:42:32 +0000 |
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1 # ARGalaxy Immune Repertoire |
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2 This is the GitHub repository for the ARGalaxy Immune repertoire pipeline. |
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3 The Galaxy tool version can be found [here](https://toolshed.g2.bx.psu.edu/repository/browse_repositories_i_own?sort=name&operation=view_or_manage_repository&id=2e457d63170a4b1c). |
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4 The docker version can be found [here](https://github.com/ErasmusMC-Bioinformatics/ARGalaxy-docker). |
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5 |
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6 ## Overview |
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7 |
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8 In execution order: |
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9 |
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10 #### imgt_loader or igblast |
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11 |
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12 ###### imgt_loader (Recommended) |
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13 Start the analysis with [IMGT HighV Quest](https://www.imgt.org/HighV-QUEST/) archives. |
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14 An IMGT archive file holds [multiple tabular files](http://www.imgt.org/IMGT_vquest/share/textes/imgtvquest.html#output3), this script extracts the specific columns relevant to the analysis from several of these files. |
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15 |
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16 `Rscript imgt_loader.r 1_Summary.txt 3_Nt-sequences.txt 5_AA-sequences.txt 6_Junction.txt 4_IMGT-gapped-AA-sequences.txt /path/to/output.txt` |
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17 |
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18 |
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19 ###### igblast |
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20 Start the analysis with FASTA files that are aligned with [igblast](https://www.ncbi.nlm.nih.gov/igblast/). |
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21 Note that this method will provide less information than the IMGT archive. |
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22 |
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23 `sh igblast.sh /path/to/sequences.fasta species locus /path/to/output.txt` |
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24 |
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25 #### experimental_design |
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26 This script will merge multiple result files (from the last step) into a single file with an additional ID and Replicate column to differentiate the individual samples during the analysis and to allow for analysis across samples. |
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27 |
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28 `Rscript experimental_design.r /path/to/input_1 id_1 [/path/to/input_2 id_2] [/path/to/input_n id_n] /path/to/output` |
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29 |
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30 #### report_clonality |
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31 The R script that creates the analysis result. |
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32 |
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33 `sh r_wrapper.sh /path/to/experimental_design/output.txt /path/to/output_dir/output.html /path/to/output_dir "clonaltype" "species" "locus" "filter_productive" "clonality_method"` |
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34 |
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35 ###### parameters |
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36 Clonaltype: |
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37 - none |
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38 - Top.V.Gene,CDR3.Seq |
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39 - Top.V.Gene,CDR3.Seq.DNA |
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40 - Top.V.Gene,Top.J.Gene,CDR3.Seq |
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41 - Top.V.Gene,Top.J.Gene,CDR3.Seq.DNA |
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42 - Top.V.Gene,Top.D.Gene,Top.J.Gene,CDR3.Seq.DNA |
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43 |
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44 Species: |
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45 - Homo sapiens functional |
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46 - Homo sapiens |
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47 - Homo sapiens non-functional |
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48 - Bos taurus |
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49 - Bos taurus functional |
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50 - Bos taurus non-functional |
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51 - Camelus dromedarius |
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52 - Camelus dromedarius functional |
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53 - Camelus dromedarius non-functional |
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54 - Canis lupus familiaris |
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55 - Canis lupus familiaris functional |
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56 - Canis lupus familiaris non-functional |
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57 - Danio rerio |
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58 - Danio rerio functional |
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59 - Danio rerio non-functional |
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60 - Macaca mulatta |
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61 - Macaca mulatta functional |
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62 - Macaca mulatta non-functional |
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63 - Mus musculus |
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64 - Mus musculus functional |
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65 - Mus musculus non-functional |
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66 - Mus spretus |
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67 - Mus spretus functional |
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68 - Mus spretus non-functional |
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69 - Oncorhynchus mykiss |
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70 - Oncorhynchus mykiss functional |
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71 - Oncorhynchus mykiss non-functional |
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72 - Ornithorhynchus anatinus |
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73 - Ornithorhynchus anatinus functional |
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74 - Ornithorhynchus anatinus non-functional |
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75 - Oryctolagus cuniculus |
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76 - Oryctolagus cuniculus functional |
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77 - Oryctolagus cuniculus non-functional |
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78 - Rattus norvegicus |
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79 - Rattus norvegicus functional |
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80 - Rattus norvegicus non-functional |
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81 - Sus scrofa |
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82 - Sus scrofa functional |
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83 - Sus scrofa non-functional |
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84 |
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85 Locus: |
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86 - TRA |
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87 - TRD |
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88 - TRG |
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89 - TRB |
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90 - IGH |
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91 - IGI |
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92 - IGK |
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93 - IGL |
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94 |
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95 Filter productive: |
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96 - yes |
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97 - no |
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98 |
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99 Clonality Method: |
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100 - none |
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101 - old |
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102 - boyd |
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103 |
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104 ## complete.sh |
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105 This script will run all of the above for you, it will detect if you are using FASTA files or IMGT archives and use the appropriate tools. |
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106 |
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107 `sh complete.sh /path/to/input_1 id_1 [/path/to/input_n id_n] /path/to/out_dir/out.html /path/to/out_dir clonaltype species locus filter_productive clonality_method` |
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108 See "report_clonality" for the parameter options. |
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109 |
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110 ## Dependencies |
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111 - Linux |
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112 - R |
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113 - gridExtra |
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114 - ggplot2 |
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115 - plyr |
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116 - data.table |
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117 - reshape2 |
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118 - lymphclon |
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119 |
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120 #### optional |
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121 - Circos |
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122 - IgBlast |
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123 - igblastwrp |