Mercurial > repos > davidvanzessen > shm_csr
annotate shm_clonality.htm @ 83:729738462297 draft
"planemo upload commit c0ffc68aec5836d5b20b543106493056a87edf57"
author | rhpvorderman |
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date | Wed, 15 Sep 2021 12:24:06 +0000 |
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1 <html> |
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3 <head> |
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4 <meta http-equiv=Content-Type content="text/html; charset=windows-1252"> |
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5 <meta name=Generator content="Microsoft Word 14 (filtered)"> |
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6 <style> |
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7 <!-- |
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8 /* Font Definitions */ |
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9 @font-face |
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10 {font-family:Calibri; |
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11 panose-1:2 15 5 2 2 2 4 3 2 4;} |
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12 @font-face |
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13 {font-family:Tahoma; |
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14 panose-1:2 11 6 4 3 5 4 4 2 4;} |
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15 /* Style Definitions */ |
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16 p.MsoNormal, li.MsoNormal, div.MsoNormal |
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17 {margin-top:0in; |
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18 margin-right:0in; |
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19 margin-bottom:10.0pt; |
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20 margin-left:0in; |
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21 line-height:115%; |
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22 font-size:11.0pt; |
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23 font-family:"Calibri","sans-serif";} |
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24 a:link, span.MsoHyperlink |
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25 {color:blue; |
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26 text-decoration:underline;} |
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27 a:visited, span.MsoHyperlinkFollowed |
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28 {color:purple; |
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29 text-decoration:underline;} |
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30 p |
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31 {margin-right:0in; |
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32 margin-left:0in; |
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33 font-size:12.0pt; |
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34 font-family:"Times New Roman","serif";} |
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35 p.MsoAcetate, li.MsoAcetate, div.MsoAcetate |
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36 {mso-style-link:"Balloon Text Char"; |
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37 margin:0in; |
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38 margin-bottom:.0001pt; |
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39 font-size:8.0pt; |
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40 font-family:"Tahoma","sans-serif";} |
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41 p.msochpdefault, li.msochpdefault, div.msochpdefault |
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42 {mso-style-name:msochpdefault; |
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43 margin-right:0in; |
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44 margin-left:0in; |
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45 font-size:12.0pt; |
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46 font-family:"Calibri","sans-serif";} |
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47 p.msopapdefault, li.msopapdefault, div.msopapdefault |
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48 {mso-style-name:msopapdefault; |
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49 margin-right:0in; |
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50 margin-bottom:10.0pt; |
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51 margin-left:0in; |
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52 line-height:115%; |
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53 font-size:12.0pt; |
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54 font-family:"Times New Roman","serif";} |
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55 span.apple-converted-space |
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56 {mso-style-name:apple-converted-space;} |
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57 span.BalloonTextChar |
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58 {mso-style-name:"Balloon Text Char"; |
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59 mso-style-link:"Balloon Text"; |
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60 font-family:"Tahoma","sans-serif";} |
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61 .MsoChpDefault |
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62 {font-size:10.0pt; |
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63 font-family:"Calibri","sans-serif";} |
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64 .MsoPapDefault |
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65 {margin-bottom:10.0pt; |
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66 line-height:115%;} |
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67 @page WordSection1 |
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68 {size:8.5in 11.0in; |
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69 margin:1.0in 1.0in 1.0in 1.0in;} |
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70 div.WordSection1 |
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71 {page:WordSection1;} |
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72 --> |
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73 </style> |
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74 |
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75 </head> |
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76 |
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77 <body lang=EN-US link=blue vlink=purple> |
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78 |
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79 <div class=WordSection1> |
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80 |
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81 <p style='margin-top:0in;margin-right:0in;margin-bottom:6.4pt;margin-left:0in; |
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82 text-align:justify;background:white'><b><span lang=EN-GB style='color:black'>References</span></b></p> |
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83 |
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84 <p style='margin-top:0in;margin-right:0in;margin-bottom:6.4pt;margin-left:0in; |
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85 text-align:justify;background:white'><span lang=EN-GB style='color:black'>Gupta, |
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86 Namita T. and Vander Heiden, Jason A. and Uduman, Mohamed and Gadala-Maria, |
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87 Daniel and Yaari, Gur and Kleinstein, Steven H. (2015). <a name="OLE_LINK106"></a><a |
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88 name="OLE_LINK107"></a>Change-O: a toolkit for analyzing large-scale B cell |
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89 immunoglobulin repertoire sequencing data: Table 1. In<span |
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90 class=apple-converted-space> </span><em>Bioinformatics, 31 (20), pp. |
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91 3356–3358.</em><span class=apple-converted-space><i> </i></span>[</span><a |
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92 href="http://dx.doi.org/10.1093/bioinformatics/btv359" target="_blank"><span |
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93 lang=EN-GB style='color:#303030'>doi:10.1093/bioinformatics/btv359</span></a><span |
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94 lang=EN-GB style='color:black'>][</span><a |
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95 href="http://dx.doi.org/10.1093/bioinformatics/btv359" target="_blank"><span |
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96 lang=EN-GB style='color:#303030'>Link</span></a><span lang=EN-GB |
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97 style='color:black'>]</span></p> |
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98 |
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99 <p style='margin-top:0in;margin-right:0in;margin-bottom:6.4pt;margin-left:0in; |
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100 text-align:justify;background:white'><span lang=EN-GB style='color:black'> </span></p> |
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101 |
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102 <p style='margin-top:0in;margin-right:0in;margin-bottom:6.4pt;margin-left:0in; |
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103 text-align:justify;background:white'><a name="OLE_LINK110"><u><span lang=EN-GB |
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104 style='color:black'>All, IGA, IGG, IGM and IGE tabs</span></u></a></p> |
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105 |
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106 <p style='margin-top:0in;margin-right:0in;margin-bottom:6.4pt;margin-left:0in; |
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107 text-align:justify;background:white'><span lang=EN-GB style='color:black'>In |
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108 these tabs information on the clonal relation of transcripts can be found. To |
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109 calculate clonal relation Change-O is used (Gupta et al, PMID: 26069265). |
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110 Transcripts are considered clonally related if they have maximal three nucleotides |
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111 difference in their CDR3 sequence and the same first V segment (as assigned by |
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112 IMGT). Results are represented in a table format showing the clone size and the |
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113 number of clones or sequences with this clone size. Change-O settings used are |
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114 the </span><span lang=EN-GB>nucleotide hamming distance substitution model with |
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115 a complete distance of maximal three. For clonal assignment the first gene |
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116 segments were used, and the distances were not normalized. In case of |
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117 asymmetric distances, the minimal distance was used.<span style='color:black'> </span></span></p> |
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118 |
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119 <p style='margin-top:0in;margin-right:0in;margin-bottom:6.4pt;margin-left:0in; |
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120 text-align:justify;background:white'><span lang=EN-GB style='color:black'> </span></p> |
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121 |
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122 <p style='margin-top:0in;margin-right:0in;margin-bottom:6.4pt;margin-left:0in; |
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123 text-align:justify;background:white'><u><span lang=EN-GB style='color:black'>Overlap |
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124 tab</span></u><span lang=EN-GB style='color:black'> </span></p> |
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125 |
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126 <p style='margin-top:0in;margin-right:0in;margin-bottom:6.4pt;margin-left:0in; |
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127 text-align:justify;background:white'><span lang=EN-GB style='color:black'>This |
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128 tab gives information on with which (sub)classe(s) each unique analyzed region |
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129 (based on the exact nucleotide sequence of the analyzes region and the CDR3 |
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130 nucleotide sequence) is found with. This gives information if the combination |
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131 of the exact same nucleotide sequence of the analyzed region and the CDR3 |
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132 sequence can be found in multiple (sub)classes.</span></p> |
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133 |
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134 <p style='margin-top:0in;margin-right:0in;margin-bottom:6.4pt;margin-left:0in; |
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135 text-align:justify;background:white'><span style='color:black'><img src="data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAA8AAAAPCAYAAAA71pVKAAAAzElEQVQoka2TwQ2CQBBFpwTshw4ImW8ogJMlUIMmhNCDxgasAi50oSXA8XlAjCG7aqKTzGX/vsnM31mzR0gk7tTudO5MEizpzvQ4ryUSe408J3Xn+grE0p1rnpOamVmWsZG4rS+dzzAMsN8Hi9yyjI1JNGtxu4VxBJgLRLpoTKIPiW0LlwtUVRTubW2OBGUJu92cZRmdfbKQMAw8o+vi5v0fLorZ7Y9waGYJjsf38DJz0O1PsEQffOcv4Sa6YYfDDJ5Obzbsp93+5VfdATueO1fdLdI0AAAAAElFTkSuQmCC"> Please note that this tab is based on all |
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136 sequences before filter unique sequences and the remove duplicates based on |
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137 filters are applied. In this table only sequences occuring more than once are |
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138 included. </span></p> |
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139 |
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140 </div> |
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141 |
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142 </body> |
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143 |
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144 </html> |