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view mass.py @ 13:6926716bbfb5 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/cufflinks/cufflinks commit 80b06e80066b32ad53ed418628992f056444256f
author | iuc |
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date | Sat, 05 Oct 2024 11:16:09 +0000 |
parents | d080005cffe1 |
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import shutil import sys import tempfile def parse_gff_attributes(attr_str): """ Parses a GFF/GTF attribute string and returns a dictionary of name-value pairs. The general format for a GFF3 attributes string is name1=value1;name2=value2 The general format for a GTF attribute string is name1 "value1" ; name2 "value2" The general format for a GFF attribute string is a single string that denotes the interval's group; in this case, method returns a dictionary with a single key-value pair, and key name is 'group' """ attributes_list = attr_str.split(";") attributes = {} for name_value_pair in attributes_list: # Try splitting by '=' (GFF3) first because spaces are allowed in GFF3 # attribute; next, try double quotes for GTF. pair = name_value_pair.strip().split("=") if len(pair) == 1: pair = name_value_pair.strip().split("\"") if len(pair) == 1: # Could not split for some reason -- raise exception? continue if pair == '': continue name = pair[0].strip() if name == '': continue # Need to strip double quote from values value = pair[1].strip(" \"") attributes[name] = value if len(attributes) == 0: # Could not split attributes string, so entire string must be # 'group' attribute. This is the case for strictly GFF files. attributes['group'] = attr_str return attributes def gff_attributes_to_str(attrs, gff_format): """ Convert GFF attributes to string. Supported formats are GFF3, GTF. """ if gff_format == 'GTF': format_string = '%s "%s"' # Convert group (GFF) and ID, parent (GFF3) attributes to transcript_id, gene_id id_attr = None if 'group' in attrs: id_attr = 'group' elif 'ID' in attrs: id_attr = 'ID' elif 'Parent' in attrs: id_attr = 'Parent' if id_attr: attrs['transcript_id'] = attrs['gene_id'] = attrs[id_attr] elif gff_format == 'GFF3': format_string = '%s=%s' attrs_strs = [] for name, value in attrs.items(): attrs_strs.append(format_string % (name, value)) return " ; ".join(attrs_strs) stderr = sys.argv[1] global_model_file_name = sys.argv[2] transcripts = sys.argv[3] # Read standard error to get total map/upper quartile mass. total_map_mass = -1 with open(stderr, 'r') as tmp_stderr2: for line in tmp_stderr2: if line.lower().find("map mass") >= 0 or line.lower().find("upper quartile") >= 0: total_map_mass = float(line.split(":")[1].strip()) break if global_model_file_name != "None": # Global model is simply total map mass from original run. with open(global_model_file_name, 'r') as global_model_file: global_model_total_map_mass = float(global_model_file.readline()) # Ratio of global model's total map mass to original run's map mass is # factor used to adjust FPKM. fpkm_map_mass_ratio = total_map_mass / global_model_total_map_mass # Update FPKM values in transcripts.gtf file. with open(transcripts, 'r') as transcripts_file: with tempfile.NamedTemporaryFile(dir=".", delete=False) as new_transcripts_file: for line in transcripts_file: fields = line.split('\t') attrs = parse_gff_attributes(fields[8]) attrs["FPKM"] = str(float(attrs["FPKM"]) * fpkm_map_mass_ratio) attrs["conf_lo"] = str(float(attrs["conf_lo"]) * fpkm_map_mass_ratio) attrs["conf_hi"] = str(float(attrs["conf_hi"]) * fpkm_map_mass_ratio) fields[8] = gff_attributes_to_str(attrs, "GTF") new_transcripts_file.write("%s\n" % '\t'.join(fields)) shutil.move(new_transcripts_file.name, transcripts) if total_map_mass > -1: with open("global_model.txt", 'w') as f: f.write("%f\n" % total_map_mass)