Mercurial > repos > devteam > fastq_paired_end_joiner
diff fastq_paired_end_joiner.xml @ 1:270a8ed8a300 draft
Uploaded tool version 2.0.0 by Simone Leo.
author | devteam |
---|---|
date | Mon, 07 Jul 2014 15:37:28 -0400 |
parents | 2793d1d765b9 |
children | 41ab1243e8f9 |
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--- a/fastq_paired_end_joiner.xml Mon Jan 27 09:25:44 2014 -0500 +++ b/fastq_paired_end_joiner.xml Mon Jul 07 15:37:28 2014 -0400 @@ -1,12 +1,16 @@ -<tool id="fastq_paired_end_joiner" name="FASTQ joiner" version="1.0.0"> +<tool id="fastq_paired_end_joiner" name="FASTQ joiner" version="2.0.0"> <description>on paired end reads</description> <requirements> <requirement type="package" version="1.0.0">galaxy_sequence_utils</requirement> </requirements> - <command interpreter="python">fastq_paired_end_joiner.py '$input1_file' '${input1_file.extension[len( 'fastq' ):]}' '$input2_file' '${input2_file.extension[len( 'fastq' ):]}' '$output_file'</command> + <command interpreter="python">fastq_paired_end_joiner.py '$input1_file' '${input1_file.extension[len( 'fastq' ):]}' '$input2_file' '${input2_file.extension[len( 'fastq' ):]}' '$output_file' '$style'</command> <inputs> <param name="input1_file" type="data" format="fastqsanger,fastqcssanger" label="Left-hand Reads" /> <param name="input2_file" type="data" format="fastqsanger,fastqcssanger" label="Right-hand Reads" /> + <param name="style" type="select" label="FASTQ Header Style"> + <option value="old" selected="true">old</option> + <option value="new">new</option> + </param> </inputs> <outputs> <data name="output_file" format="input" /> @@ -21,14 +25,19 @@ <help> **What it does** -This tool joins paired end FASTQ reads from two separate files into a single read in one file. The join is performed using sequence identifiers, allowing the two files to contain differing ordering. If a sequence identifier does not appear in both files, it is excluded from the output. - -Sequence identifiers with /1 and /2 appended override the left-hand and right-hand designation; i.e. if the reads end with /1 and /2, the read containing /1 will be used as the left-hand read and the read containing /2 will be used as the right-hand read. Sequences without this designation will follow the left-hand and right-hand settings set by the user. +This tool joins paired end FASTQ reads from two separate files into a +single read in one file. The join is performed using sequence +identifiers, allowing the two files to contain differing ordering. If +a sequence identifier does not appear in both files, it is excluded +from the output. ----- **Input formats** +Both old and new (from recent Illumina software) style FASTQ headers +are supported. The following example uses the "old" style. + Left-hand Read:: @HWI-EAS91_1_30788AAXX:7:21:1542:1758/1 @@ -56,10 +65,26 @@ ------ -**Citation** +**The "new" style** + +Recent Illumina FASTQ headers are structured as follows:: + + @COORDS FLAGS + COORDS = INSTRUMENT:RUN_#:FLOWCELL_ID:LANE:TILE:X:Y + FLAGS = READ:IS_FILTERED:CONTROL_NUMBER:INDEX_SEQUENCE + +where the whitespace character between COORDS and FLAGS can be either +a space or a tab. -If you use this tool, please cite `Blankenberg D, Gordon A, Von Kuster G, Coraor N, Taylor J, Nekrutenko A; Galaxy Team. Manipulation of FASTQ data with Galaxy. Bioinformatics. 2010 Jul 15;26(14):1783-5. <http://www.ncbi.nlm.nih.gov/pubmed/20562416>`_ +------ + +**Credits** +This is an extended version (adds support for "new" style FASTQ headers) +of D. Blankenberg's fastq joiner: +`Blankenberg D, Gordon A, Von Kuster G, Coraor N, Taylor J, Nekrutenko A; Galaxy Team. Manipulation of FASTQ data with Galaxy. Bioinformatics. 2010 Jul 15;26(14):1783-5. <http://www.ncbi.nlm.nih.gov/pubmed/20562416>`_ + +New style header support added by Simone Leo <simone.leo@crs4.it> </help> </tool>