##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	1000gsample.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5000
Sequences flagged as poor quality	0
Sequence length	54
%GC	43
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.9946	31.0	27.0	33.0	22.0	34.0
2	28.486	30.0	26.0	32.0	21.0	33.0
3	28.5272	30.0	26.0	32.0	21.0	34.0
4	28.6252	30.0	26.0	32.0	22.0	33.0
5	28.4804	30.0	26.0	32.0	21.0	33.0
6	28.5128	30.0	26.0	32.0	22.0	33.0
7	28.4102	30.0	26.0	32.0	21.0	33.0
8	28.4674	30.0	26.0	32.0	22.0	33.0
9	28.182	30.0	26.0	32.0	21.0	33.0
10	28.0908	30.0	26.0	32.0	21.0	33.0
11	28.1876	30.0	26.0	32.0	21.0	33.0
12	28.1742	30.0	26.0	32.0	21.0	33.0
13	27.923	30.0	25.0	32.0	20.0	33.0
14	27.8984	29.0	25.0	32.0	21.0	33.0
15	27.6112	29.0	25.0	32.0	20.0	33.0
16	27.6638	29.0	25.0	32.0	20.0	33.0
17	27.5734	29.0	25.0	32.0	20.0	33.0
18	27.6348	29.0	25.0	32.0	20.0	33.0
19	27.6526	29.0	25.0	32.0	20.0	33.0
20	27.2716	29.0	24.0	32.0	19.0	33.0
21	27.2642	29.0	25.0	31.0	19.0	33.0
22	26.881	28.0	24.0	31.0	19.0	33.0
23	26.8124	28.0	24.0	31.0	19.0	33.0
24	26.8204	28.0	24.0	31.0	19.0	33.0
25	26.6606	28.0	24.0	31.0	18.0	33.0
26	26.3806	28.0	23.0	31.0	18.0	33.0
27	25.947	28.0	23.0	31.0	17.0	33.0
28	25.7446	27.0	22.0	31.0	16.0	33.0
29	25.6856	27.0	22.0	31.0	16.0	33.0
30	25.5228	27.0	22.0	31.0	16.0	33.0
31	25.354	27.0	22.0	30.0	16.0	32.0
32	25.1656	27.0	22.0	30.0	16.0	32.0
33	25.0164	26.0	21.0	30.0	15.0	32.0
34	24.75	26.0	21.0	30.0	15.0	32.0
35	24.3926	26.0	21.0	30.0	15.0	32.0
36	24.1122	25.0	20.0	30.0	14.0	32.0
37	23.8612	25.0	20.0	29.0	13.0	32.0
38	23.7544	25.0	20.0	29.0	13.0	32.0
39	23.485	25.0	19.0	29.0	13.0	32.0
40	23.1984	24.0	19.0	29.0	13.0	32.0
41	23.0976	24.0	19.0	29.0	13.0	32.0
42	22.9212	24.0	19.0	28.0	13.0	31.0
43	22.5172	24.0	18.0	28.0	12.0	31.0
44	22.3234	23.0	18.0	28.0	12.0	31.0
45	22.1372	23.0	18.0	28.0	11.0	31.0
46	22.2166	23.0	18.0	28.0	11.0	31.0
47	22.075	23.0	18.0	28.0	11.0	31.0
48	21.4592	22.0	17.0	27.0	11.0	31.0
49	21.0954	22.0	16.0	27.0	10.0	30.0
50	20.9356	22.0	16.0	27.0	10.0	30.0
51	20.6152	21.0	15.0	27.0	9.0	30.0
52	20.6588	21.0	16.0	27.0	9.0	30.0
53	19.5852	20.0	14.0	25.0	9.0	29.0
54	19.9424	21.0	14.0	26.0	7.0	30.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1	1	0.4542485249902164
1	2	0.02493171505959424
1	3	0.004493134032774293
1	4	0.6119404948560927
1	5	0.5283275008594686
1	6	0.3741933003058371
1	7	-0.2985554485298749
1	8	0.6040181642739206
1	9	0.43795745033596845
1	10	0.05327706110314523
1	11	0.8097461135057031
1	12	0.9171964750225641
1	13	1.0716973449965757
1	14	0.562649938864773
1	15	0.5112535943737946
1	16	0.7098461441939676
1	17	0.3989886413671577
1	18	0.2351551176870892
1	19	0.002716666680861124
1	20	-0.5270840838615776
1	21	0.08959510509382795
1	22	-0.6322238602545553
1	23	-0.8566085860715056
1	24	-0.8167218970288026
1	25	0.7728199138173544
1	26	-0.26898877195552373
1	27	-0.7734079986509634
1	28	0.46313879073469266
1	29	-1.0803714983510417
1	30	-0.9107347156963463
1	31	-1.1279249650605188
1	32	0.03588141895047414
1	33	-0.3276409211703317
1	34	0.07833890179158587
1	35	-0.8393131051954583
1	36	-1.024889600311596
1	37	-1.4177418858492992
1	38	-1.912918888443187
1	39	-1.880569234686881
1	40	-0.9901560786471286
1	41	-1.9499635516515283
1	42	-2.3754765893384793
1	43	-1.6682056704307335
1	44	-2.2432919652101226
1	45	-2.1214550638335794
1	46	-1.6941624871292404
1	47	-2.261740586778952
1	48	-0.47246064535067234
1	49	-1.345180096970381
1	50	-1.3012642855865373
1	51	-1.6748103331735038
1	52	-1.4936238782530324
1	53	-0.9900326328970834
1	54	-0.9042724595665739
2	1	0.27305194379363584
2	2	-0.40803531790744074
2	3	-1.054114924575284
2	4	0.02463890755450393
2	5	-1.0899875174555511
2	6	-0.7630472369346997
2	7	-1.2563088062832328
2	8	0.09022085047660511
2	9	-0.8821890698105506
2	10	-1.0053309975049132
2	11	-1.2879339841743942
2	12	-2.149225991399902
2	13	-1.2225639492647176
2	14	-0.7472401710253394
2	15	-0.9942409111207091
2	16	-1.07061783627001
2	17	-1.0825742401957257
2	18	-1.0554431729112004
2	19	-1.374084310120118
2	20	-0.25260300938050406
2	21	-0.7384879229891972
2	22	-0.5973032253339206
2	23	-1.926205655668575
2	24	-1.8018256821325878
2	25	-1.2574609164634758
2	26	-2.588402691369442
2	27	-1.909427534670499
2	28	-2.213051685455781
2	29	-3.343130961110507
2	30	-3.447731052692685
2	31	-3.261502498638052
2	32	-2.596108813039759
2	33	-2.173306366835778
2	34	-2.968059144606464
2	35	-3.110863776746129
2	36	-3.6207381961601897
2	37	-2.8287308968383122
2	38	-2.3431997187240157
2	39	-2.7157340698517167
2	40	-2.8763587648498152
2	41	-3.221758423446399
2	42	-3.735916149778042
2	43	-2.8279126301376927
2	44	-2.034012355930514
2	45	-3.745875088253605
2	46	-3.8848828778496305
2	47	-3.6639383889767565
2	48	-3.0197133926034176
2	49	-3.1119688637591487
2	50	-2.7486391329613866
2	51	-2.4279238862870542
2	52	-3.3607789454081
2	53	-4.239849482713934
2	54	-3.3794861347802474
3	1	0.4228103978999158
3	2	1.0474512245356244
3	3	0.4013716178677811
3	4	-0.7648710993468057
3	5	1.642931737202833
3	6	0.42190790454218075
3	7	0.5808202482371243
3	8	0.22341615758495692
3	9	0.06605109582092084
3	10	1.203778733343949
3	11	-0.3886931284117985
3	12	0.31385199341721304
3	13	0.7829570997345918
3	14	-0.2772608749590866
3	15	-0.2613216564623251
3	16	-0.6475674566978924
3	17	-1.0549689951991752
3	18	-1.3066509023798005
3	19	-0.22225546263909735
3	20	-1.028310393783542
3	21	-0.3112967566675984
3	22	-0.2177311066313692
3	23	1.415409251163723
3	24	1.0256415366389788
3	25	-0.5776817584234486
3	26	0.41573809538003914
3	27	1.098163907703551
3	28	0.5095156023288965
3	29	0.6313341872676865
3	30	0.4032006243259474
3	31	-0.21733410664357322
3	32	-0.1723683023427256
3	33	-0.8558460493754616
3	34	0.4094425807213504
3	35	1.0478663819840293
3	36	2.193616531238014
3	37	0.2458032646523698
3	38	0.5829562509102146
3	39	-0.23954359366124223
3	40	-0.6762207386248349
3	41	1.2420096924956283
3	42	1.0798188398699935
3	43	0.9132881980196572
3	44	0.7368641105981268
3	45	0.004297444527622929
3	46	1.1033848930268384
3	47	0.008939457814136631
3	48	-1.3137092518166682
3	49	-0.5681455373271262
3	50	1.1098839864312993
3	51	-0.835791381111072
3	52	-0.5478044802597211
3	53	-0.49036708105762017
3	54	0.06495830966419547
4	1	0.05082972157141441
4	2	0.528472618600496
4	3	-0.06998794044829992
4	4	0.9770198599354565
4	5	1.1004886730206387
4	6	0.1575876837002177
4	7	0.6801991302247039
4	8	0.5346652949210515
4	9	-0.2155224031438827
4	10	-0.29104528321919787
4	11	-0.7165054127458248
4	12	-1.561924404098317
4	13	-0.5241512508520181
4	14	-0.19168461546978222
4	15	-0.91487583175563
4	16	0.42144565579348026
4	17	0.7586956010741162
4	18	-0.26179237926040955
4	19	0.2449633089275025
4	20	0.5093017525242587
4	21	-0.3575355420368176
4	22	-1.5020651300958257
4	23	-0.19604692550984382
4	24	-0.008174888481793374
4	25	1.0917454327428722
4	26	0.7131846102178585
4	27	0.2493026240596592
4	28	0.9774245050204087
4	29	-0.45424207222161783
4	30	-1.4953501003117324
4	31	0.2146879775524262
4	32	0.9277007107697663
4	33	-0.26854446207387284
4	34	-0.30139247793979607
4	35	0.5240568581745073
4	36	-0.5572461326681264
4	37	0.3934913253839092
4	38	0.4345780590537629
4	39	1.0779167237990777
4	40	0.3299904414993904
4	41	0.39728919560121767
4	42	-0.5454399593018522
4	43	-2.6850554872805503
4	44	0.7913844694663119
4	45	0.8255534831749678
4	46	0.5436885507218001
4	47	-0.7591764842148514
4	48	0.5040961312061043
4	49	-0.5087942605845441
4	50	-1.3359407202629718
4	51	-0.2057016640648328
4	52	0.6868401022109474
4	53	0.26808702522257377
4	54	-0.23662899192310505
5	1	0.6850177557594499
5	2	0.48647017659805414
5	3	0.7275700571096984
5	4	0.38117126408686275
5	5	1.0514044239363898
5	6	1.066500992613527
5	7	0.9475983976239704
5	8	-0.05752029726454211
5	9	1.0994959118744312
5	10	0.6532770611031467
5	11	0.17897688273647105
5	12	0.1325810904071787
5	13	-0.05137957808034699
5	14	0.1318807080955402
5	15	-0.6425925594723587
5	16	0.15599999034781575
5	17	0.5220655644440804
5	18	0.9890012715332439
5	19	0.23348589745009107
5	20	0.10368514690765096
5	21	0.766518182016906
5	22	0.7523915243608279
5	23	-0.3643008937638115
5	24	0.2755857952788894
5	25	0.5112814522788938
5	26	-0.2997580027247544
5	27	-0.6964849217278868
5	28	0.6477541753500766
5	29	1.1350131170335729
5	30	0.6431114381498055
5	31	0.8413058041702506
5	32	-0.11796473489567916
5	33	-1.5430255365549463
5	34	0.0014158248685056662
5	35	-0.17777464365699558
5	36	0.29818732276532955
5	37	-0.1254341935416079
5	38	0.7332349577106605
5	39	0.011738457620811715
5	40	0.24061315212210133
5	41	-1.4576558593438378
5	42	0.10144648758459596
5	43	0.3933327911077278
5	44	-1.027907349825508
5	45	0.7708526284741133
5	46	-0.5557009486676989
5	47	0.2459517209133537
5	48	-0.9955375684275936
5	49	-0.11441086620115115
5	50	-0.37818736250961393
5	51	-0.22865648701965569
5	52	0.4756068909777369
5	53	0.2868904440259925
5	54	-1.0119647672588812
6	1	0.9498196205613141
6	2	-1.07903098890311
6	3	0.2142833438229843
6	4	-0.327453444537845
6	5	0.5204020929340594
6	6	-0.7269722008596666
6	7	-0.06871861869304396
6	8	-0.9992452389894844
6	9	-0.3410635286850088
6	10	0.020643028469113034
6	11	-0.022421718662130274
6	12	1.7367768946029827
6	13	-1.8257385524393221
6	14	0.8241884004032336
6	15	0.4646335477537491
6	16	0.47627971062753716
6	17	-0.18935634697783144
6	18	0.41930430183627365
6	19	1.0689171328813245
6	20	1.1297923730148796
6	21	0.6439074594061829
6	22	1.1011094730787754
6	23	-1.3461190755819956
6	24	-0.6041344844413885
6	25	0.27212061311805513
6	26	0.12588302291626974
6	27	1.011207385964422
6	28	-0.4035278759319745
6	29	1.075339457359913
6	30	2.019801414839783
6	31	0.45855522141966887
6	32	1.3375131205821766
6	33	3.2956691021396907
6	34	1.691392514845198
6	35	0.7448360789537283
6	36	1.7443411689191741
6	37	2.1193210512136353
6	38	1.125775750251453
6	39	-0.36075571487336333
6	40	0.7888649003738486
6	41	0.7479385462505697
6	42	-0.3246607385226312
6	43	1.5746847724597082
6	44	0.9068245849064276
6	45	1.3811090387305214
6	46	2.146863153896401
6	47	1.4529447279063632
6	48	0.023576650686624845
6	49	0.9601812083909245
6	50	-1.057441441763693
6	51	-0.19415765252082196
6	52	0.897518312889158
6	53	-1.4692867121511632
6	54	0.7619280066338909
7	1	-2.6562409854992914
7	2	-1.866909776781899
7	3	-0.24026211072246895
7	4	-0.20624132332572387
7	5	0.247674820206786
7	6	-0.4239418978293621
7	7	-0.765688315662743
7	8	-0.18106342080766424
7	9	0.8407546531331711
7	10	0.4448854527115387
7	11	-0.47696717320758353
7	12	-1.7177685599424706
7	13	-0.795435522136291
7	14	-0.7515691753543408
7	15	0.16160324472344456
7	16	-0.5843263499785252
7	17	0.5379163802948952
7	18	0.41930430183627365
7	19	-1.5068404428762499
7	20	-1.5065712633487571
7	21	0.674210489709214
7	22	0.9192912912605955
7	23	1.3811536516907346
7	24	0.9110170307101235
7	25	-1.4248490838516439
7	26	0.6410345380677853
7	27	-0.3221259473689102
7	28	-1.130800603204701
7	29	0.4692788512993076
7	30	-0.7680773730390058
7	31	-1.1778084149439678
7	32	-1.1473353642663078
7	33	-0.5225127160421259
7	34	-1.217698394245712
7	35	-0.43698210286445516
7	36	-0.8617194371414314
7	37	-0.8503759184833335
7	38	-1.4196787952030938
7	39	0.7301533760357302
7	40	0.7585618700708174
7	41	1.7479385462505697
7	42	1.0389756251137356
7	43	1.2716544694294072
7	44	0.8462185243003688
7	45	0.5023211599426425
7	46	-1.2167732097399622
7	47	0.9074901824518165
7	48	1.1750918022017771
7	49	0.56624181445153
7	50	0.6698312855090336
7	51	2.2300847717216037
7	52	0.4429728583437047
7	53	2.561016318151868
7	54	1.5498067945126799
8	1	-0.014529755552768364
8	2	0.3398638417564257
8	3	-0.884647137460437
8	4	-0.06453009337920079
8	5	-0.7006317751586337
8	6	-0.567435658970183
8	7	-0.3940305616520483
8	8	-0.3584252746401084
8	9	-0.09507422387217446
8	10	-1.100569092743008
8	11	-0.5250955154535717
8	12	0.009504167330256053
8	13	1.1938692906979327
8	14	1.000658988638527
8	15	0.6402128703918954
8	16	1.2944615288093537
8	17	-0.04942764822560264
8	18	1.183118918592065
8	19	0.842535671205745
8	20	0.47382089351398804
8	21	-0.23755421617313743
8	22	-0.6145768014310278
8	23	-0.7231244231755767
8	24	-0.6094820780242785
8	25	-0.5968633441011981
8	26	0.4984320425241151
8	27	0.27591326831736396
8	28	-0.3545082680888356
8	29	0.7847868726896827
8	30	1.3575910761588545
8	31	2.432708519102377
8	32	0.17886784428984015
8	33	1.3402323819970903
8	34	0.3045832456829878
8	35	-0.4031140101728319
8	36	0.3619882277427031
8	37	0.4401766661868969
8	38	1.317397853638262
8	39	0.5447701318289546
8	40	-0.6924185220860437
8	41	1.582163145181049
8	42	1.4489577998017893
8	43	0.8919753250443812
8	44	1.0173415189527724
8	45	1.1654227642207182
8	46	1.5194121735042465
8	47	2.5545490059812295
8	48	1.6777655989932185
8	49	0.3380778215816562
8	50	1.5263375243682127
8	51	-0.8929098806848152
8	52	1.233525443013935
8	53	0.5262569598630975
8	54	2.859075956723018
9	1	-1.2602813895396956
9	2	-0.5699400798122021
9	3	-0.4160196864800447
9	4	-0.2769483940327966
9	5	-1.8391938666619012
9	6	0.16393689004942402
9	7	0.004008654034226566
9	8	-0.611366451110694
9	9	-0.7996493872708683
9	10	-0.9450135371874531
9	11	1.1088914126510012
9	12	1.7428375006635903
9	13	0.1964836697829
9	14	-1.0424782662634335
9	15	0.3676638507840515
9	16	-1.349982915635092
9	17	0.45710829948681564
9	18	-0.5443320618000911
9	19	-0.16138589742170595
9	20	0.4045398477623543
9	21	-0.03690062140189809
9	22	0.6566650286343325
9	23	1.2801435506806307
9	24	1.0140473337404288
9	25	1.0155549565523962
9	26	-0.4963391993059503
9	27	-1.144348169591133
9	28	-0.29241676482086376
9	29	0.018773800794257056
9	30	0.5935387885771561
9	31	-0.04245487959043359
9	32	1.076907059976115
9	33	1.2108206172912048
9	34	0.16209958555226933
9	35	-0.3299113957937472
9	36	0.6554522800302856
9	37	0.16174529363787826
9	38	-0.29240606793036505
9	39	-0.46176581588346366
9	40	-0.17477146326251614
9	41	-0.3995362012241799
9	42	0.40059178672989404
9	43	-0.7929919952170579
9	44	-0.9578218797400382
9	45	0.6699979276194128
9	46	0.124640931674179
9	47	0.2408235157851486
9	48	-0.37844355133357865
9	49	0.3419993902091072
9	50	-0.8756232599455132
9	51	0.44826658990341883
9	52	0.34398295935380574
9	53	0.5347536918892395
9	54	-1.9350416903358045
10	1	1.0952741660158587
10	2	1.4967265868544644
10	3	1.317313646853286
10	4	-0.3547261718105723
10	5	-1.4614160888841212
10	6	0.29727022338276043
10	7	0.570675320700893
10	8	0.7553002155559696
10	9	-0.11076049838197832
10	10	0.9660975739236584
10	11	1.3200025237621134
10	12	0.5761708339969225
10	13	1.1742614475606779
10	14	0.49085506706990145
10	15	0.6676638507840522
10	16	0.5944615288093544
10	17	-0.2984472560687408
10	18	-0.07766539513342252
10	19	0.871947435911629
10	20	0.6934287366512422
10	21	-0.4924561769574538
10	22	0.13444280641211037
10	23	1.3356991062361878
10	24	0.6140473337404266
10	25	0.1933327343301734
10	26	1.2592163562496026
10	27	2.2112073859644212
10	28	1.7964721240680284
10	29	0.7632182452387006
10	30	1.604649899688269
10	31	1.8797673426317907
10	32	0.47690705997611715
10	33	-0.15584604937545876
10	34	1.8398773633300465
10	35	2.981199715317363
10	36	0.8110078355858406
10	37	1.8617452936378776
10	38	1.7742605987363014
10	39	3.2937897396720928
10	40	3.2918952034041524
10	41	1.3115749098869323
10	42	2.911702897841007
10	43	2.9292302270051636
10	44	1.964400342482186
10	45	0.5477757053971892
10	46	1.9135298205630704
10	47	1.2741568491184836
10	48	2.799334226444202
10	49	3.441999390209105
10	50	4.391043406721156
10	51	3.7815999232367545
10	52	1.3217607371315836
10	53	3.0125314696670173
10	54	2.2316249763308633
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
8	4.0
9	4.0
10	2.0
11	2.0
12	4.0
13	6.0
14	4.0
15	11.0
16	20.0
17	50.0
18	98.0
19	149.0
20	210.0
21	275.0
22	367.0
23	425.0
24	518.0
25	540.0
26	539.0
27	532.0
28	468.0
29	360.0
30	234.0
31	122.0
32	51.0
33	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.5	32.64	25.919999999999998	19.939999999999998
2	24.04	31.72	26.540000000000003	17.7
3	21.58	28.860000000000003	29.42	20.14
4	21.58	28.28	28.9	21.240000000000002
5	21.16	29.720000000000002	28.32	20.8
6	22.38	29.24	25.72	22.66
7	21.34	30.320000000000004	28.24	20.1
8	21.58	29.060000000000002	27.939999999999998	21.42
9	20.3	28.48	28.58	22.64
10	21.2	30.84	26.56	21.4
11	21.14	28.64	27.279999999999998	22.939999999999998
12	21.32	28.88	28.999999999999996	20.8
13	20.36	29.220000000000002	28.939999999999998	21.48
14	20.02	29.2	29.220000000000002	21.560000000000002
15	20.200000000000003	29.220000000000002	28.38	22.2
16	20.34	29.38	29.299999999999997	20.979999999999997
17	20.599999999999998	29.14	28.499999999999996	21.759999999999998
18	20.599999999999998	27.92	29.4	22.08
19	21.66	28.46	28.58	21.3
20	20.0	29.24	28.96	21.8
21	21.72	28.499999999999996	28.18	21.6
22	20.52	30.880000000000003	27.3	21.3
23	20.72	28.9	29.720000000000002	20.66
24	21.12	28.62	27.58	22.68
25	21.099999999999998	29.060000000000002	29.160000000000004	20.68
26	22.18887555022009	28.391356542617046	28.131252501000397	21.288515406162464
27	21.344268853770753	28.525705141028208	28.765753150630125	21.364272854570913
28	19.34	30.54	27.46	22.66
29	20.90418083616723	29.12582516503301	29.045809161832363	20.924184836967395
30	21.099999999999998	29.4	27.439999999999998	22.06
31	20.84	28.04	28.660000000000004	22.46
32	19.847939175670266	28.371348539415763	28.7515006002401	23.02921168467387
33	22.084416883376676	28.62572514502901	27.265453090618124	22.024404880976196
34	21.304260852170433	29.285857171434287	27.265453090618124	22.144428885777156
35	21.084216843368676	29.425885177035404	28.225645129025807	21.264252850570113
36	21.404280856171233	28.665733146629325	28.805761152230446	21.124224844968996
37	20.888355342136855	29.43177270908363	28.53141256502601	21.1484593837535
38	21.864372874574915	29.02580516103221	28.065613122624526	21.044208841768352
39	21.928771508603443	28.251300520208083	27.050820328131252	22.769107643057225
40	21.32	28.499999999999996	27.82	22.36
41	21.673003802281368	28.116870122073244	27.61656994196518	22.59355613368021
42	22.29337602561537	28.036822093255953	27.71662997798679	21.953171903141886
43	21.244248849769953	28.425685137027408	27.825565113022606	22.504500900180034
44	21.404280856171233	28.985797159431886	27.545509101820365	22.064412882576516
45	21.86	29.28	26.740000000000002	22.12
46	21.26	27.54	27.74	23.46
47	21.628651460584233	28.271308523409367	28.051220488195277	22.048819527811126
48	21.279999999999998	28.439999999999998	26.400000000000002	23.880000000000003
49	21.044208841768352	29.045809161832363	27.185437087417487	22.724544908981798
50	21.82873149259704	28.15126050420168	26.810724289715886	23.209283713485394
51	21.028411364545818	27.73109243697479	28.011204481792717	23.229291716686674
52	21.824364872974595	30.146029205841167	25.94518903780756	22.084416883376676
53	21.164232846569313	28.325665133026607	28.045609121824366	22.464492898579717
54	21.53907976692787	27.024311834438418	26.90375728350412	24.5328511151296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	5.0
2	7.0
3	7.0
4	7.0
5	5.0
6	2.0
7	1.0
8	1.5
9	2.0
10	1.5
11	1.0
12	2.5
13	4.0
14	4.0
15	4.0
16	5.0
17	6.0
18	11.0
19	19.5
20	23.0
21	37.5
22	52.0
23	61.0
24	70.0
25	79.0
26	88.0
27	117.5
28	147.0
29	159.5
30	172.0
31	203.0
32	243.0
33	252.0
34	290.0
35	328.0
36	330.0
37	332.0
38	332.5
39	333.0
40	342.0
41	351.0
42	343.5
43	336.0
44	318.5
45	279.5
46	258.0
47	254.5
48	251.0
49	234.5
50	218.0
51	209.5
52	201.0
53	198.5
54	196.0
55	177.0
56	162.5
57	167.0
58	147.0
59	127.0
60	106.5
61	86.0
62	85.0
63	84.0
64	61.5
65	39.0
66	42.5
67	46.0
68	34.0
69	17.0
70	12.0
71	14.0
72	16.0
73	12.5
74	9.0
75	6.5
76	4.0
77	4.5
78	5.0
79	5.0
80	5.0
81	3.0
82	1.0
83	1.0
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	1.0
90	2.0
91	3.0
92	2.5
93	2.0
94	2.0
95	3.0
96	4.0
97	5.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.04
27	0.02
28	0.0
29	0.02
30	0.0
31	0.0
32	0.04
33	0.02
34	0.02
35	0.02
36	0.02
37	0.04
38	0.02
39	0.04
40	0.0
41	0.06
42	0.06
43	0.02
44	0.02
45	0.0
46	0.0
47	0.04
48	0.0
49	0.02
50	0.04
51	0.04
52	0.02
53	0.02
54	0.45999999999999996
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
54	5000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.52
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.81913183279742	99.33999999999999
2	0.08038585209003216	0.16
3	0.04019292604501608	0.12
4	0.0	0.0
5	0.02009646302250804	0.1
6	0.0	0.0
7	0.04019292604501608	0.27999999999999997
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT	7	0.13999999999999999	Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC	7	0.13999999999999999	Illumina Paired End PCR Primer 2 (100% over 35bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.02	0.0	0.0
3	0.02	0.0	0.0
4	0.02	0.0	0.0
5	0.02	0.0	0.0
6	0.02	0.0	0.0
7	0.02	0.0	0.0
8	0.02	0.0	0.0
9	0.02	0.0	0.0
10	0.02	0.0	0.0
11	0.02	0.0	0.0
12	0.02	0.0	0.0
13	0.02	0.0	0.0
14	0.02	0.0	0.0
15	0.02	0.0	0.0
16	0.02	0.0	0.0
17	0.02	0.0	0.0
18	0.02	0.0	0.0
19	0.06	0.0	0.0
20	0.36	0.0	0.0
21	0.36	0.0	0.0
22	0.36	0.0	0.0
23	0.36	0.0	0.0
24	0.36	0.0	0.0
25	0.4	0.0	0.0
26	0.4	0.0	0.0
27	0.42	0.0	0.0
28	0.62	0.0	0.0
29	0.66	0.0	0.0
30	0.68	0.0	0.0
31	0.68	0.0	0.0
32	0.68	0.0	0.0
33	0.68	0.0	0.0
34	0.7	0.0	0.0
35	0.76	0.0	0.0
36	0.8	0.0	0.0
37	0.88	0.0	0.0
38	1.0	0.0	0.0
39	1.12	0.0	0.0
40	1.22	0.0	0.0
41	1.38	0.0	0.0
42	1.48	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE