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annotate kraken.xml @ 5:b2392a9dfb01 draft

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planemo upload for repository https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken/ commit c5dd035dbee6021f3555755cf4c814e760cecd09
author devteam
date Wed, 23 Mar 2016 10:55:18 -0400
parents 7c41dbaa9875
children 2fdac3e78553
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1 <?xml version="1.0"?>
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2 <tool id="kraken" name="Kraken" version="1.2.1">
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3 <description>
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4 assign taxonomic labels to sequencing reads
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5 </description>
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6 <macros>
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7 <import>macros.xml</import>
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8 </macros>
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9 <expand macro="requirements" />
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10 <expand macro="stdio" />
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11 <expand macro="version_command" />
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12 <command>
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13 <![CDATA[
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14 @SET_DATABASE_PATH@ &&
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15 kraken --threads \${GALAXY_SLOTS:-1} @INPUT_DATABASE@
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16
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17 ${only_classified_output}
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19 #if str( $quick_operation.quick ) == "yes":
0f17a8816b28 planemo upload for repository https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken/ commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
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20 --quick
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21 --min-hits ${quick_operation.min_hits}
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23 #end if
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24
1
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25 #if $single_paired.single_paired_selector == "yes":
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26 #if $forward_input.is_of_type( 'fastq' ):
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27 --fastq-input
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28 #else:
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29 --fasta-input
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30 #end if
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31 "$forward_input" "$reverse_input"
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32 ${single_paired.check_names}
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33 #elif $single_paired.single_paired_selector == "collection":
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34 #if $single_paired.input_pair.forward.is_of_type( 'fastq' ):
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35 --fastq-input
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36 #else:
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37 --fasta-input
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38 #end if
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39 "${single_paired.input_pair.forward}" "${single_paired.input_pair.reverse}"
642f30185af2 planemo upload for repository https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken/ commit a1517c9d22029095120643bbe2c8fa53754dd2b7
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40 ${single_paired.check_names}
1
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41 #else:
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42 #if $input_sequences.is_of_type( 'fastq' ):
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43 --fastq-input
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44 #else:
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45 --fasta-input
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46 #end if
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47 "$input_sequences"
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48 #end if
0
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49
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50 #if $split_reads:
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51 --classified-out "${classified_out}" --unclassified-out "${unclassified_out}"
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52 #end if
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53
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54 ## The --output option was changed to redirect as it does not work properly is some situations. For example, on test database the tool classifies 4 reads but does not write them into a file if --output is specified. It does however print correct output into STDOUT. This behavior can be re-created with test database provided in test-data/test_db/ folder. This is the reason for incrementing version number from 1.1.2 to 1.1.3
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55
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56 > "${output}"
0
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57 ##kraken-translate --db ${kraken_database.fields.name} "${output}" > "${translated}"
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58 ]]>
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59 </command>
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60 <inputs>
1
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61 <conditional name="single_paired">
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62 <param name="single_paired_selector" type="select" label="Single or paired reads" help="--paired">
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63 <option value="collection">Collection</option>
3
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64 <option value="yes">Paired</option>
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65 <option selected="True" value="no">Single</option>
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66 </param>
2
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67 <when value="collection">
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68 <param format="fasta,fastq" label="Collection of paired reads" name="input_pair" type="data_collection" collection_type="paired" help="FASTA or FASTQ datasets" />
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69 <param name="check_names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match" help="--check-names" />
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70 </when>
1
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71 <when value="yes">
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72 <param format="fasta,fastq" label="Forward strand" name="forward_input" type="data" help="FASTA or FASTQ dataset"/>
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73 <param format="fasta,fastq" label="Reverse strand" name="reverse_input" type="data" help="FASTA or FASTQ dataset"/>
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74 <param name="check_names" type="boolean" checked="False" truevalue="--paired --check-names" falsevalue="--paired" label="Verify read names match" help="--check-names" />
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75 </when>
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76 <when value="no">
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77 <param format="fasta,fastq" label="Input sequences" name="input_sequences" type="data" help="FASTA or FASTQ datasets"/>
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78 </when>
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79
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80 </conditional>
0
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81 <param label="Output classified and unclassified reads?" name="split_reads" type="boolean" help="Sets --unclassified-out and --classified-out"/>
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82
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83 <conditional name="quick_operation">
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84 <param name="quick" type="select" label="Enable quick operation?" help="--quick; Rather than searching all k-mers in a sequence, stop classification after a specified number of database hit">
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85 <option value="yes">Yes</option>
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86 <option selected="True" value="no">No</option>
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87 </param>
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88 <when value="yes">
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89 <param name="min_hits" type="integer" value="1" label="Number of hits required for classification" help="--min-hits; min-hits will allow you to require multiple hits before declaring a sequence classified, which can be especially useful with custom databases when testing to see if sequences either do or do not belong to a particular genome; default=1"/>
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90 </when>
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91 <when value="no">
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92 <!-- Do absolutely nothing -->
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93 </when>
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94 </conditional>
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95
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96 <param name="only_classified_output" type="boolean" checked="False" truevalue="--only-classified-output" falsevalue="" label="Print no Kraken output for unclassified sequences" help="--only-classified-output"/>
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97
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98 <expand macro="input_database" />
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99 </inputs>
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100 <outputs>
1
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101 <data format_source="input_sequences" label="${tool.name} on ${on_string}: Classified reads" name="classified_out">
0
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102 <filter>(split_reads)</filter>
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103 </data>
1
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104 <data format_source="input_sequences" label="${tool.name} on ${on_string}: Unclassified reads" name="unclassified_out">
0
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105 <filter>(split_reads)</filter>
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106 </data>
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107 <data format="tabular" label="${tool.name} on ${on_string}: Classification" name="output" />
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108 <!--<data format="tabular" label="${tool.name} on ${on_string}: Translated classification" name="translated" />-->
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109 </outputs>
3
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110
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111 <tests>
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112 <test>
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113 <param name="single_paired_selector" value="no"/>
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114 <param name="input_sequences" value="kraken_test1.fa" ftype="fasta"/>
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115 <param name="split_reads" value="false"/>
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116 <param name="quick" value="no"/>
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117 <param name="only-classified-output" value="false"/>
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118 <param name="kraken_database" value="test_db"/>
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119 <output name="output" file="kraken_test1_output.tab" ftype="tabular"/>
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120 </test>
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121 </tests>
0
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122 <help>
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123 <![CDATA[
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124 **What it does**
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125
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126 Kraken is a taxonomic sequence classifier that assigns taxonomic labels to short DNA reads. It does this by examining the k-mers within a read and querying a database with those k-mers. This database contains a mapping of every k-mer in Kraken's genomic library to the lowest common ancestor (LCA) in a taxonomic tree of all genomes that contain that k-mer. The set of LCA taxa that correspond to the k-mers in a read are then analyzed to create a single taxonomic label for the read; this label can be any of the nodes in the taxonomic tree. Kraken is designed to be rapid, sensitive, and highly precise.
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127
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128 -----
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129
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130 **Kraken options**
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131
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132 The Galaxy version of Kraken implements the following options::
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133
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134
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135 --fasta-input Input is FASTA format
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136 --fastq-input Input is FASTQ format
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137 --quick Quick operation (use first hit or hits)
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138 --min-hits NUM In quick op., number of hits req'd for classification
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139 NOTE: this is ignored if --quick is not specified
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140 --unclassified-out Print unclassified sequences to filename
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141 --classified-out Print classified sequences to filename
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142
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143 --only-classified-output Print no Kraken output for unclassified sequences
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144
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145 ------
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146
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147 **Output Format**
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148
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149 Each sequence classified by Kraken results in a single line of output. Output lines contain five tab-delimited fields; from left to right, they are::
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150
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151 1. "C"/"U": one letter code indicating that the sequence was either classified or unclassified.
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152 2. The sequence ID, obtained from the FASTA/FASTQ header.
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153 3. The taxonomy ID Kraken used to label the sequence; this is 0 if the sequence is unclassified.
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154 4. The length of the sequence in bp.
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155 5. A space-delimited list indicating the LCA mapping of each k-mer in the sequence. For example, "562:13 561:4 A:31 0:1 562:3" would indicate that:
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156 a) the first 13 k-mers mapped to taxonomy ID #562
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157 b) the next 4 k-mers mapped to taxonomy ID #561
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158 c) the next 31 k-mers contained an ambiguous nucleotide
0f17a8816b28 planemo upload for repository https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken/ commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
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159 d) the next k-mer was not in the database
0f17a8816b28 planemo upload for repository https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken/ commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
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160 e) the last 3 k-mers mapped to taxonomy ID #562
0f17a8816b28 planemo upload for repository https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken/ commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
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161 ]]>
0f17a8816b28 planemo upload for repository https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken/ commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
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162 </help>
0f17a8816b28 planemo upload for repository https://github.com/galaxyproject/tools-devteam/blob/master/tool_collections/kraken/kraken/ commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
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163 <expand macro="citations" />
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164 </tool>