annotate lastz.xml @ 7:10aca14c2332 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
author iuc
date Thu, 17 May 2018 04:29:13 -0400
parents b6d7308c3728
children e7f19d6a9af8
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10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
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1 <tool id="lastz_wrapper_2" name="LASTZ" version="1.3.1">
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2 <description>: align long sequences</description>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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3 <macros>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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4 <import>lastz_macros.xml</import>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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5 </macros>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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6 <requirements>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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7 <requirement type="package" version="@LASTZ_CONDA_VERSION@">lastz</requirement>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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8 <requirement type="package" version="1.7">samtools</requirement>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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9 <requirement type="package" version="3.4.2">r-base</requirement>
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10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
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10 <requirement type="package" version="1.0.6">bzip2</requirement>
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11 </requirements>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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12 <command detect_errors="exit_code"><![CDATA[
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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13 lastz
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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14 @TARGET_INPUT_COMMAND_LINE@
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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15 ## If --self is set: perform self alignment and ignore the query
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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16 #if $where_to_look.self:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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17 '${where_to_look.self}'
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18 #if $where_to_look.nomirror
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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19 '${where_to_look.nomirror}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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20 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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21 #else:
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10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
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22 @query_input@
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bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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23 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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24
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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25 ## WHERE TO LOOK --------------------------------
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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26
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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27 '${where_to_look.strand}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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28 #if str( $where_to_look.qhsplimit.qhsplimit_selector ) == "yes":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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29 #if $where_to_look.qhsplimit.qhsplimit_n:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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30 #if $where_to_look.qhsplimit.qhsplimit_nowarn:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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31 #if $where_to_look.qhsplimit.qhsplimit_keep:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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32 '--queryhsplimit=${where_to_look.qhsplimit.qhsplimit_keep},${where_to_look.qhsplimit.qhsplimit_nowarn}:${where_to_look.qhsplimit.qhsplimit_n}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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33 #else:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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34 '--queryhsplimit=${where_to_look.qhsplimit.qhsplimit_nowarn}:${where_to_look.qhsplimit.qhsplimit_n}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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35 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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36 #else:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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37 '--queryhsplimit=${where_to_look.qhsplimit.qhsplimit_n}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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38 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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39 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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40 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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41
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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42 #if $where_to_look.qhspbest:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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43 '--queryhspbest=${where_to_look.qhspbest}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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44 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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45
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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46 #if str( $where_to_look.qdepth.qdepth_selector ) == "yes":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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47 #if $where_to_look.qdepth.qdepth_n:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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48 #if $where_to_look.qdepth.qdepth_nowarn and not $where_to_look.qdepth.qdepth_keep:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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49 '--querydepth=${where_to_look.qdepth.qdepth_nowarn}:${where_to_look.qdepth.qdepth_n}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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50 #elif not $where_to_look.qdepth.qdepth_nowarn and $where_to_look.qdepth.qdepth_keep:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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51 '--querydepth=${where_to_look.qdepth.qdepth_keep}:${where_to_look.qdepth.qdepth_n}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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52 #elif $where_to_look.qdepth.qdepth_nowarn and $where_to_look.qdepth.qdepth_keep:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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53 '--querydepth=${where_to_look.qdepth.qdepth_keep},${where_to_look.qdepth.qdepth_nowarn}:${where_to_look.qdepth.qdepth_n}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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54 #else:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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55 '--querydepth=${where_to_look.qdepth.qdepth_n}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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56 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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57 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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58 #end if
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59
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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60 ## SCORING --------------------------------
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61
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62 #if $scoring.score_file:
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63 '--scores=${scoring.score_file}'
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64 #end if
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65
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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66 #if str( $scoring.match.match_selector ) == "yes":
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67 #if $scoring.match.match_reward:
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68 #if $scoring.match.match_penalty:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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69 '--match=${scoring.match.match_reward},${scoring.match.match_penalty}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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70 #else:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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71 '--match=${scoring.match.match_reward}'
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72 #end if
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73 #end if
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74 #end if
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75 #if str( $scoring.gap.gap_selector ) == "yes":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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76 #if $scoring.gap.gap_extend:
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77 #if $scoring.gap.gap_open:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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78 '--gap=${scoring.gap.gap_open},${scoring.gap.gap_extend}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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79 #else:
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80 '--gap=${scoring.gap.gap_extend}'
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81 #end if
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82 #end if
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83 #end if
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84 #if $scoring.ambigN:
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85 '${scoring.ambigN}'
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86 #end if
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87 #if $scoring.ambigIUPAC:
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88 '${scoring.ambigIUPAC}'
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89 #end if
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90
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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91 ## SEEDING --------------------------------
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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92
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93 #if str( $seeding.seed.seed_selector ) == "pre_set":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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94 '${seeding.seed.pre_set_seeds}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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95 #elif str( $seeding.seed.seed_selector ) == "len":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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96 '--seed=match${seeding.seed.seed_match}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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97 #elif str( $seeding.seed.seed_selector ) == "half_len":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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98 '--seed=half${seeding.seed.seed_half}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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99 #elif str( $seeding.seed.seed_selector ) == "pattern":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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100 '--seed=${seeding.seed.pattern}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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101 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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102 #if str( $seeding.transitions ) != "--transition":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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103 '${seeding.transitions}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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104 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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105 #if str( $seeding.seed_filer.seed_filer_selector ) == "yes":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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106 #if $seeding.seed_filer.filter_match:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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107 #if $seeding.seed_filer.filter_tr:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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108 '--filter=${seeding.seed_filer.filter_tr},${seeding.seed_filer.filter_match}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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109 #else:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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110 '--filter=${seeding.seed_filer.filter_match}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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111 #end if
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112 #end if
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113 #end if
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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114
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115 ## FINDING HSPs --------------------------------
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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116
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117 #if $hsp.gfextend:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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118 '${hsp.gfextend}'
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119 #end if
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120 #if $hsp.nogfextend
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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121 '${hsp.nogfextend}'
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122 #end if
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123 #if str( $hsp.hsp_method.hsp_method_selector ) == "match":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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124
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125 #if $hsp.hsp_method.exact:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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126 '--exact=${hsp.hsp_method.exact}'
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127 #end if
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128 #elif str( $hsp.hsp_method.hsp_method_selector ) == "mismatch":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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129
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130 #if $hsp.hsp_method.mismatch_count and $hsp.hsp_method.mismatch_length:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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131 '--mismatch=${hsp.hsp_method.mismatch_count},${hsp.hsp_method.mismatch_length}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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132 #end if
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133 #elif str( $hsp.hsp_method.hsp_method_selector ) == "x":
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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134
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135 #if $hsp.hsp_method.xdrop:
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136 '--xdrop=${hsp.hsp_method.xdrop}'
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137 #end if
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138
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139 #if $hsp.hsp_method.hspthresh:
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140 '--hspthresh=${hsp.hsp_method.hspthresh}'
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141 #end if
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142
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143 #if $hsp.hsp_method.hspthresh_top:
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144 '--hspthresh=top${hsp.hsp_method.hspthresh_top}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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145 #end if
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146
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147 #if $hsp.hsp_method.hspthresh_top_percent:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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148 '--hspthresh=top${hsp.hsp_method.hspthresh_top_percent}%'
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149 #end if
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150 #end if
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151 #if $hsp.entropy:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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152 '${hsp.entropy}'
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153 #end if
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154 #if $hsp.entropy_report:
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155 '${hsp.entropy_report}'
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156 #end if
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157 #if $hsp.noentropy:
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158 '${hsp.noentropy}'
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159 #end if
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160
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161 ## CHAINING --------------------------------
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162
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163 #if $chaining.chain:
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164 '${chaining.chain}'
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165 #end if
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166 #if str( $chaining.chaning_penalties.chaning_penalties_selector ) == "yes":
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167 #if $chaining.chaning_penalties.diag and $chaining.chaning_penalties.anti:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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168 '--chain=${chaining.chaning_penalties.diag},${chaining.chaning_penalties.anti}'
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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169 #end if
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170 #end if
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171
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172 ## GAPPED EXTENSION --------------------------------
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173
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174 #if $gap_ext.gapped:
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175 '${gap_ext.gapped}'
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176 #end if
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177 #if $gap_ext.nogapped:
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178 '${gap_ext.nogapped}'
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179 #end if
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180 #if $gap_ext.ydrop:
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181 '--ydrop=${gap_ext.ydrop}'
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182 #end if
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183 #if $gap_ext.noytrim:
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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184 '${gap_ext.noytrim}'
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185 #end if
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devteam
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186 #if $gap_ext.gappedthresh:
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187 '--gappedthresh=${gap_ext.gappedthresh}'
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188 #end if
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189 #if $gap_ext.allgappedbounds:
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190 '${gap_ext.allgappedbounds}'
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191 #end if
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192
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193 ## FILTERING --------------------------------
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194
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195 #if $filters.identity.id_min:
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196 #if $filters.identity.id_max:
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197 '--filter=identity:${filters.identity.id_min}..${filters.identity.id_max}'
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198 #else:
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199 '--filter=identity:${filters.identity.id_min}'
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200 #end if
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201 #end if
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202 #if $filters.continuity.cont_min:
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203 #if $filters.continuity.cont_max:
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204 '--filter=continuity:${filters.continuity.cont_min}..${filters.continuity.cont_max}'
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205 #else:
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206 '--filter=continuity:${filters.continuity.cont_min}'
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207 #end if
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208 #end if
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209 #if $filters.coverage.cov_min:
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210 #if $filters.coverage.cov_max:
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211 '--filter=coverage:${filters.coverage.cov_min}..${filters.coverage.cov_max}'
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212 #else:
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213 '--filter=coverage:${filters.coverage.cov_min}'
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214 #end if
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215 #end if
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216 #if $filters.filter_nmatch:
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217 '--filter=nmatch:${filters.filter_nmatch}'
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218 #end if
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219 #if $filters.filter_nmatch_percent:
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220 '--filter=nmatch:${filters.filter_nmatch_percent}%'
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221 #end if
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222 #if $filters.filter_nmismatch:
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223 '--filter=nmismatch:0..${filters.filter_nmismatch}'
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224 #end if
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225 #if $filters.filter_ngap:
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226 '--filter=ngap:0..${filters.filter_ngap}'
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227 #end if
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228 #if $filters.filter_cgap:
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229 '--filter=cgap:0..${filters.filter_cgap}'
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230 #end if
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231 #if $filters.notrivial:
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232 '${filters.notrivial}'
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233 #end if
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234
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235 ## INTERPOLATION --------------------------------
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236
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237 #if $interpolation.inner:
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238 '--inner=${interpolation.inner}'
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239 #end if
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240
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241 ## OUTPUT FORMATS --------------------------------
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242
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243 #if str( $output_format.out.format ) == "bam":
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244 '--format=${output_format.out.bam_options}'
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245 #elif str( $output_format.out.format ) == "general_def":
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246 --format=general-
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247 #elif str( $output_format.out.format ) == "maf":
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248 '--format=${output_format.out.maf_type}'
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249 #elif str( $output_format.out.format ) == "blastn":
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250 --format=BLASTN-
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251 #elif str( $output_format.out.format ) == "general_full":
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252 '--format=general-:${output_format.out.fields}'
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253 #end if
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254 --action:target=multiple
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255 --rdotplot=plot.r
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256 #if str( $output_format.out.format ) == "bam":
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257 | samtools sort -@\${GALAXY_SLOTS:-2} -O bam -o '${output}' &&
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258 #else:
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259 > '${output}' &&
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260 #end if
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261 Rscript $r_plot > /dev/null 2>&1
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262
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263 ]]>
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264 </command>
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265 <configfiles>
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266 <configfile name="r_plot">
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267 <![CDATA[
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268 #!/usr/bin/env Rscript
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269 ## Setup R error handling to go to stderr
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270 options( show.error.messages=F, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } )
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271 ## Only produce image when 'plot.r' actually contains data
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272 if(file.info('plot.r')\$size>0){
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273 x <- read.table("plot.r", as.is=TRUE)
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274 png("${out_plot}", width=640, height=640, res=72)
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275 plot(x, type='l', xlab="target", ylab="query", main="plot")
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276 dev.off()
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277 }
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278 ]]>
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279 </configfile>
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280 </configfiles>
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281 <inputs>
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282 <expand macro="target_input"/>
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283 <param name="query" format="fasta,fastq,fasta.gz,fastq.gz,fastq.bz2" type="data" label="Select QUERY sequence(s)" help="These are the sequences that you are aligning against TARGET"/>
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284
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285 <section name="where_to_look" expanded="False" title="Where to look">
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286 <param name="strand" type="select" display="radio" label="which strand to search" argument="--strand" help="Search both strands or choose plus or minus">
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287 <option value="--strand=both" selected="True">Both</option>
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288 <option value="--strand=plus">Plus</option>
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289 <option value="--strand=minus">Minus</option>
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290 </param>
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291 <param name="self" type="boolean" display="radio" truevalue="--self" falsevalue="" checked="False" argument="--self" label="Perform a self-alignment: the target sequence is also the query." help="Computation is more efficient than it would be without this option, since only one of each mirror-image pair of alignment blocks is processed (the other, redundant one is skipped during processing, but re-created in the output). Also, the trivial self-alignment block along the main diagonal is omitted from the output. THIS OPTION CANNOT BE USED IF THE TARGET IS COMPRIZED OF MULTIPLE SEQUENCES"/>
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292 <param name="nomirror" type="boolean" display="radio" truevalue="--nomirror" falsevalue="" checked="False" label="Inhibit the re-creation of mirror-image alignments." argument="--nomirror" help="Output consists of only one copy of each meaningful alignment block in a self-alignment. This option is only applicable when the ‑‑self option is used."/>
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293 <conditional name="qhsplimit">
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294 <param name="qhsplimit_selector" type="select" display="radio" label="Set HSP limit" argument="--queryhsplimit">
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295 <option value="yes">Yes</option>
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296 <option value="no" selected="true">No</option>
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297 </param>
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298 <when value="yes">
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299 <param name="qhsplimit_n" optional="true" type="integer" label="Discard queries that have more than N HSPs" help="Any queries that exceed this limit are reported as a warning (to stderr), and no alignments are reported. This is useful for mapping reads to a reference genome, when some reads align to too many places in the reference."/>
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300 <param name="qhsplimit_nowarn" type="boolean" truevalue="nowarn" display="radio" checked="False" label=" Withhold warnings for queries that exceed the limit set above"/>
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301 <param name="qhsplimit_keep" type="boolean" truevalue="keep" display="radio" checked="False" label="Keep queries that exceed the limit and supress warnings" help="For such a query, the first N HSPs found are passed along to downstream processing. Note that the HSPs reported are not the best N HSPs. They are simply the first N found; they very likely have a positional bias."/>
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302 </when>
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303 <when value="no">
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304 <!-- Do nothing -->
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305 </when>
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306 </conditional>
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307 <param name="qhspbest" type="integer" optional="true" label="For queries that have more than N HSPs, discard any HSPs that score below the Nth best." help="This is useful for mapping reads to a reference genome, when some reads align to too many places in the reference."/>
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308 <conditional name="qdepth">
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309 <param name="qdepth_selector" type="select" display="radio" label="Set ratio of aligned bases to query length" argument="--querydepth">
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310 <option value="yes">Yes</option>
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311 <option value="no" selected="true">No</option>
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312 </param>
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313 <when value="yes">
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314 <param name="qdepth_n" optional="true" type="integer" label="Stop processing gapped alignments for a query/strand if its ratio of aligned bases to query length exceeds" help="This is a real number that corresponds to a depth of coverage threshold. For example, a value of 5.0 would cause termination once a query/strand has an average of five alignments for every base in the query. The numerator is the number of matches or substitutions (but not gaps); the denominator is the length of the query sequence. The purpose of this option is one of saving time. It is useful for automatically terminating the processing of queries with high repeat content, for which other methods of dealing with repetitive content fail. Moreover, back-end filtering options are not considered. In other words, matches are counted for any alignment that meets the scoring threshold, regardless of whether that alignment would be reported. The justification is that we are trying to abort the processing of queries that have too many bounding alignments in the DP matrix, and back-end filtering occurs later in the process."/>
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315 <param name="qdepth_keep" type="boolean" truevalue="keep" display="radio" checked="False" label="Warnings for queries that exceed the limit are witheld" help="Note that the alignments reported are not guaranteed to be the highest scoring alignments that would achieve the threshold. They are simply the first alignments found. In other words, the purpose of this option is one of saving time, not one of finding optimal alignments."/>
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316 <param name="qdepth_nowarn" type="boolean" truevalue="nowarn" display="radio" checked="False" label="Same as above but any alignments discovered for this query/strand, before it exceeds the threshold, are reported."/>
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317 </when>
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318 <when value="no">
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319 <!-- Do nothing -->
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320 </when>
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321 </conditional>
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322 </section>
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323 <section name="scoring" expanded="false" title="Scoring">
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324 <param name="score_file" type="data" format="txt" optional="true" argument="--scores" label="Read the substitution scores and gap penalties (and possibly other options) from a scoring file (see help below for file fomat description)." help="This option cannot be used in conjunction with ‑‑match or inference."/> <!--TODO EDIT INFERENCE -->
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325 <conditional name="match">
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326 <param name="match_selector" type="select" display="radio" label="Set the score values for a match and mismatch" argument="--match" help="Note that specifying ‑‑match changes the defaults for some of the other options (e.g. the scoring penalties for gaps, and various extension thresholds), as described in respective sections of LASTZ manual. The regular defaults are chosen for compatibility with BLASTZ, but since BLASTZ doesn't support ‑‑match, LASTZ infers that you are not expecting BLASTZ compatibility for this run, so it is free to use improved defaults. THIS OPTION CANNOT BE USED WITH --scores">
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327 <option value="yes">Yes</option>
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328 <option value="no" selected="true">No</option>
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329 </param>
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330 <when value="yes">
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331 <param name="match_reward" type="integer" optional="true" label="Score value for a match"/>
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332 <param name="match_penalty" type="integer" optional="true" label="Score value for a mismatch"/>
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333 </when>
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334 <when value="no">
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335 <!-- Do nothing -->
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336 </when>
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337 </conditional>
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338 <conditional name="gap">
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339 <param name="gap_selector" type="select" display="radio" label="Set the score penalties for opening and extending a gap" argument="--gap" help="These are specified as positive values; subtraction is implicitly assumed. Note that the first base in a gap incurs the sum of both penalties. This option is only valid if gapped extension is being performed, and cannot be used in conjunction with inference. These values specified on the command line override any corresponding values from a file provided with ‑‑scores.">
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340 <option value="yes">Yes</option>
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341 <option value="no" selected="true">No</option>
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342 </param>
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343 <when value="yes">
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344 <param name="gap_open" type="integer" optional="true" label="Gap opening penalty"/>
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345 <param name="gap_extend" type="integer" optional="true" label="Gap extension penalty"/>
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346 </when>
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347 <when value="no">
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348 <!-- Do nothing -->
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349 </when>
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350 </conditional>
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351 <param name="ambigN" type="boolean" truevalue="--ambiguous=n" checked="false" label="Treat each N in the input sequences as an ambiguous nucleotide" argument="--ambiguous=n" help="Substitutions with N are scored as zero, instead of using the fill_score value from the scoring file (which is -100 by default)."/>
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352 <param name="ambigIUPAC" type="boolean" truevalue="--ambiguous=iupac" checked="false" label="Treat each of the IUPAC-IUB ambiguity codes (B, D, H, K, M, R, S, V, W, and Y, as well as N) in the input sequences as a completely ambiguous nucleotide." argument="--ambiguous=iupac" help="Substitutions with these characters are scored as zero, instead of using the fill_score value from the scoring file (which is -100 by default)."/>
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353 </section>
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354 <section name="seeding" expanded="false" title="Seeding">
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355 <conditional name="seed">
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356 <param name="seed_selector" type="select" display="radio" label="Select seed type">
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357 <option value="defaults" selected="true">Use defaults</option>
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358 <option value="pre_set">Use pre-set values</option>
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359 <option value="len">Set length</option>
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360 <option value="half_len">Set half length</option>
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361 <option value="pattern">Set pattern</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
362 </param>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
363 <when value="defaults">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
364 <!-- Do nothing -->
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
365 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
366 <when value="pre_set">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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367 <param name="pre_set_seeds" type="select" display="radio" label="Select seed pattern" help="Select between 12of19 seed (19-bp word with matches in 12 specific positions: 1110100110010101111) or 14of22 seed (22-bp word with matches in 14 specific positions: 1110101100110010101111). 0 = mismatch, 1 = match" argument="--seed=12of19,--seed=14of22">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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368 <option value="--seed=12of19">12of19</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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369 <option value="--seed=14of22">14of22</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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370 </param>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
371 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
372 <when value="len">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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373 <param name="seed_match" type="integer" optional="true" label="Set seed length" help="Seeds require a N-bp word with matches in all positions" argument="--seed=match" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
374 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
375 <when value="half_len">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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376 <param name="seed_half" type="integer" optional="true" label="Set seed length" help="Seeds requires N-bp word with matches or transitions in all positions" argument="--seed=half" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
377 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
378 <when value="pattern">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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379 <param name="pattern" type="text" size="25" optional="true" argument="--seed=PATTERN" label="Specify an arbitrary pattern" help="Use 1s, 0s, and Ts for seed discovery (e.g., 1110100110010101111 where 1 = match, 0 = mismatch, T = transition)" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
380 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
381 </conditional>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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382 <param name="transitions" type="select" display="radio" label="Allow transitions" help="In each seed, specify how many match positions can be transition(s) instead">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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383 <option value="--transition" selected="true">One</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
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384 <option value="--transition=2">Two</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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385 <option value="--notransition">None</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
386 </param>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
387 <conditional name="seed_filer">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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388 <param name="seed_filer_selector" type="select" display="radio" label="Filter seeds" argument="--filter">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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389 <option value="yes">Yes</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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390 <option value="no" selected="true">No</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
391 </param>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
392 <when value="yes">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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393 <param name="filter_tr" type="integer" optional="true" label="Number of transitions" help="Allowing no more than this number of transversions. If not specified, any number of transversions is allowed (they are not limited)" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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394 <param name="filter_match" type="integer" optional="true" label="Number of matches" help="Require at least this many exact matches"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
395 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
396 <when value="no">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
397 <!-- Do nothing -->
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
398 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
399 </conditional>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
400 </section>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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401 <section name="hsp" expanded="false" title="HSPs (Gap-free extension)">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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402 <param name="gfextend" type="boolean" truevalue="--gfextend" checked="false" argument="--gfextend" label="Perform gap-free extension of seeds to HSPs" help="This will take into account other papermeters in this section"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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403 <param name="nogfextend" type="boolean" truevalue="--nogfextend" argument="--nogfextend" label="Skip the gap-free extension stage" help="Instead, pass the seeds along to the next specified stage.It is not recommended to use --nogfextend without also using --nogapped."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
404 <conditional name="hsp_method">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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405 <param name="hsp_method_selector" type="select" display="radio" label="Select HSP finding method">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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406 <option value="none" selected="true">None</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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407 <option value="match">Match extension</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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408 <option value="mismatch">Mismatch extension</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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409 <option value="x">X-drop extension</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
410 </param>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
411 <when value="none">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
412 <!-- Do nothing -->
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
413 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
414 <when value="match">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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415 <param name="exact" type="integer" optional="true" argument="--exact" label="Find HSPs using the exact match extension method with the given length threshold" help="This is instead of using the x-drop method"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
416 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
417 <when value="mismatch">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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418 <param name="mismatch_count" type="integer" optional="true" label="Specify number of mismatches"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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419 <param name="mismatch_length" type="integer" min="1" max="50" optional="true" label="Specify length threshold" help="Find HSPs using the mismatch extension method with the given length threshold and allowing specified number of mismatches" argument="--mismatch"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
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420 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
421 <when value="x">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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422 <param name="xdrop" type="integer" optional="true" argument="--xdrop" label="Find HSPs using the x-drop extension method with this threshold" help="The dropoff setting determines the endpoints of each gap-free segment: the extension of each seed is stopped when its cumulative score drops off by more than the given threshold from the maximum seen so far."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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423 <param name="hspthresh" type="integer" optional="true" argument="--hspthresh" label="Set the score threshold for the x-drop extension method" help="HSPs scoring lower are discarded"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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424 <param name="hspthresh_top" type="integer" optional="true" argument="--hspthresh=top" label="Set an adaptive score threshold for the x-drop extension method" help="HSPs scoring lower are discarded. The score threshold is chosen to limit the number of target sequence bases in HSPs to about this value (or possibly a little higher in case of ties, etc.)."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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425 <param name="hspthresh_top_percent" type="integer" optional="true" argument="--hspthresh=top%" label="Set an adaptive score threshold for the x-drop extension method" help="HSPs scoring lower are discarded. The score threshold is chosen to limit the number of target sequence bases in HSPs to about this perentage value (or possibly a little higher in case of ties, etc.)."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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426 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
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427 </conditional>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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428 <param name="entropy" type="boolean" truevalue="--entropy" checked="false" label="Adjust for entropy when qualifying HSPs in the x-drop extension method" help="Those that score just slightly above the HSP threshold are adjusted downward according to the entropy of their nucleotides, and any that then fall below the threshold are discarded."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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429 <param name="entropy_report" type="boolean" truevalue="--entropy=report" checked="false" label="Adjust for entropy when qualifying HSPs in the x-drop extension method, and report (to stderr) any HSPs that are discarded as a result." help="Reported HSPs are printed to stderr"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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430 <param name="noentropy" type="boolean" truevalue="--noentropy" checked="false" label="Don't adjust for entropy when qualifying HSPs"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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431 </section>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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432 <section name="chaining" expanded="false" title="Chaining">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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433 <param name="chain" type="boolean" truevalue="--chain" checked="false" argument="--chain" label="Perform chaining of HSPs with no penalties"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
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434 <conditional name="chaning_penalties">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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435 <param name="chaning_penalties_selector" type="select" display="radio" argument="--chain=" label="Perform chaining with penalties">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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436 <option value="yes">Yes</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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437 <option value="no" selected="true">No</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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438 </param>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
439 <when value="yes">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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440 <param name="diag" type="integer" optional="true" label="Penalty for diagonal in dynamic programming matrix"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
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441 <param name="anti" type="integer" optional="true" label="Penalty for anti-diagonal in dynamic programming matrix" help="These are specified as positive values; subtraction from the score is implicitly assumed."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
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442 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
443 <when value="no">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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parents:
diff changeset
444 <!-- Do nothing -->
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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445 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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446 </conditional>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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447 </section>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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448 <section name="gap_ext" expanded="false" title="Gapped extension">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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449 <param name="gapped" type="boolean" truevalue="--gapped" argument="--gapped" label="Perform gapped extension of HSPs" help="Extension of HSPs (or seeds, if gap-free extension is not performed), is performed after first reducing them to anchor points."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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450 <param name="nogapped" type="boolean" truevalue="--nogapped" argument="--nogapped" label="Skip the gapped extension stage" help="This means that interpolation must also be skipped, since it is not allowed without gapped extension"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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451 <param name="ydrop" type="integer" optional="true" argument="--ydrop" label="Set the threshold for terminating gapped extension" help="This restricts the endpoints of each local alignment by limiting the local region around each anchor in which extension is performed. The boundary of this region in the dynamic programming matrix is formed by the points where the cumulative score has dropped off by more than the given threshold from the maximum seen so far."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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452 <param name="noytrim" type="boolean" truevalue="--noytrim" argument="--noytrim" label="If y-drop extension encounters the end of the sequence, extend the alignment to the end of the sequence rather than trimming it back to the location giving the maximum score" help="This is highly recommended when either the target or query sequences are short reads (say, less than 100 bases), to prevent y-drop mismatch shadow."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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453 <param name="gappedthresh" type="integer" optional="true" argument="--gappedthresh" label="Set the threshold for gapped extension" help="Alignments scoring lower than that value are discarded. When used along with the x-drop method for gap-free extension, this value is generally set at least as high as the HSP threshold. Setting it lower has no effect, since at worst the HSP itself would always qualify (both extension stages use the same scoring matrix)."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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454 <param name="allgappedbounds" type="boolean" truevalue="--allgappedbounds" argument="--allgappedbounds" label="Revert to handling bounding alignments the way they were handled in BLASTZ."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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455 </section>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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456 <section name="filters" expanded="false" title="Filtering">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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457 <section name="identity" expanded="true" title="Filter alignments by percent identity">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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458 <param name="id_min" type="integer" min="0" max="100" optional="true" label="Minimum identity"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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459 <param name="id_max" type="integer" min="0" max="100" optional="true" argument="--filter=identity" label="Maximum identity (optional)" help="0 ≤ min ≤ max ≤ 100 percent. Identity is the percentage of aligned bases that are matches. Alignment blocks outside the given range are discarded."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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460 </section>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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461 <section name="continuity" expanded="false" title="Filter alignments by continuity">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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462 <param name="cont_min" type="integer" min="0" max="100" optional="true" label="Minimum continuity"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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463 <param name="cont_max" type="integer" min="0" max="100" optional="true" argument="--filter=continuity" label="Maximum continuity (optional)" help="0 ≤ min ≤ max ≤ 100 percent. Continuity is the percentage of alignment columns that are not gaps. Alignment blocks outside the given range are discarded."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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464 </section>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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465 <section name="coverage" expanded="false" title="Filter alignments by coverage">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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466 <param name="cov_min" type="integer" min="0" max="100" optional="true" label="Minimum coverage"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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467 <param name="cov_max" type="integer" min="0" max="100" optional="true" argument="--filter=coverage" label="Maximum coverage (optional)" help=" 0 ≤ min ≤ max ≤ 100 percent. Coverage is the percentage of the entire target or query sequence (whichever is shorter) that is included in the alignment block. Blocks outside the given range are discarded."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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468 </section>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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469 <param name="filter_nmatch" type="integer" min="1" optional="true" argument="--filter=nmatch" label="Filter alignments by how many bases match" help="Requiring at least this number of matched bases, min > 0. Match count is the number of matched bases in the alignment."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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470 <param name="filter_nmatch_percent" type="integer" min="1" optional="true" argument="--filter=nmatch%" label="Filter alignments by how many bases match expressed as percentage" help="e.g., percentage of the query length."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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471 <param name="filter_nmismatch" type="integer" min="0" optional="true" argument="--filter=nmismatch:0.." label="Filter alignments by the number of mismatches" help="Allow no more than this number of mismatched bases. Mismatch count, or nmismatch, is the number of aligned bases in the alignment that are mismatches (substitutions)."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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472 <param name="filter_ngap" type="integer" min="0" optional="true" argument="--filter=ngap:0.." label="Filter alignments by the number of gaps" help="Allow no more than this number of gaps. Gap count, or ngap, is the number of runs of gapped columns in the alignment (each run is counted as one gap)."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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473 <param name="filter_cgap" type="integer" min="0" optional="true" argument="--filter=cgap:0.." label="Filter alignments by the number of gap columns" help="Allow no more than this number of gaps. Gap column count, or cgap, is the number of gapped columns in the alignment (each column is counted as one gap)."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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474 <param name="notrivial" type="boolean" truevalue="--notrivial" argument="--notrivial" label="Do not output a trivial self-alignment block if the target and query sequences are identical" help="Note that using ‑‑self automatically enables this option."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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475 </section>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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476 <section name="interpolation" expanded="false" title="Interpolation">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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477 <param name="inner" type="integer" optional="true" argument="--inner" label="Perform additional alignment between the gapped alignment blocks, using (presumably) more sensitive alignment parameters" help="This value is used as the threshold for both the gap-free and gapped extension sub-stages; see the discussion of interpolation for more details. This option is only valid if gapped extension is performed."/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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478 </section>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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479 <section name="output_format" expanded="false" title="Output">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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480 <conditional name="out">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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481 <param name="format" type="select" display="radio" label="Specify the output format">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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482 <option value="bam" selected="true">BAM --format=sam)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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483 <option value="general_def">General default (--format=general)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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484 <option value="general_full">Customized general (‑‑format=general[:fields])</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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485 <option value="maf">MAF (--format=maf)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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486 <option value="blastn">blastn (--format=BLASTN)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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487 </param>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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488 <when value="bam">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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489 <param name="bam_options" type="select" display="radio" argument="--format=sam, --format=softsam" label="Select a BAM flavor to output" help="Lastz actually outputs SAM data but Galaxy converts it into BAM to save space. For alignments that don't reach the end of a query, ‑‑format=sam uses 'hard clipping', while ‑‑format=softsam uses 'soft clipping'. See the section on 'clipped alignment' in the SAM specification for an explanation of what this means. The options ‑‑format=sam- and ‑‑format=softsam- suppress the SAM header lines. This makes them suitable for concatenating output from multiple runs. If you need to specify readgroup information: use AddOrEplaceReadGroups from Picard package">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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490 <option value="sam" selected="true">BAM</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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491 <option value="softsam">soft-clipped BAM</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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492 <option value="sam-">BAM without header</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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493 <option value="softsam-">soft-clipped BAM without header</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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494 </param>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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495 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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496 <when value="general_def">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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497 <!-- Do nothing -->
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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498 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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499 <when value="general_full">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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500 <param name="fields" type="select" display="checkboxes" multiple="true" label="Select which fields to include" argument="--format=general-[:fields]">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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501 <option value="score" selected="true">score: Score of the alignment block</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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502 <option value="name1" selected="true">name1: Name of the target sequence</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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503 <option value="number1">number1: Number of the target sequence within the target file</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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504 <option value="strand1" selected="true">strand1: Target sequence strand </option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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505 <option value="size1" selected="true">size1: Size of the entire target sequence</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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506 <option value="start1">start1: Starting position of the alignment block in the target, origin-one</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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507 <option value="zstart1" selected="true">zstart1: Starting position of the alignment block in the target, origin-zero</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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508 <option value="end1" selected="true">end1: Ending position of the alignment block in the target</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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509 <option value="length1">length1: Length of the alignment block in the target (excluding gaps)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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510 <option value="text1">text1: Aligned characters in the target, including gap characters</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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511 <option value="qalign1">qalign1: The target quality sequence (if there is one) correpsonding to aligned characters</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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512 <option value="nucs1">nucs1: The entire target sequence</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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513 <option value="name2" selected="true">name2: Name of the query sequence</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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514 <option value="number2">number2: Number of the query sequence within the query file</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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515 <option value="strand2" selected="true">strand2: Query sequence strand</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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516 <option value="size2" selected="true">size2: Size of the entire query sequence</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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517 <option value="start2">start2: Starting position of the alignment block in the query, origin-one</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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518 <option value="zstart2" selected="true">zstart2: Starting position of the alignment block in the query, origin-one</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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519 <option value="end2" selected="true">end2: Ending position of the alignment block in the query</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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520 <option value="length2">length2: Length of the alignment block in the query (excluding gaps)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
521 <option value="text2">text2: Aligned characters in the query, including gap characters</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
522 <option value="qalign2">qalign2: The query quality sequence (if there is one) correpsonding to aligned characters</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
523 <option value="nucs2">nucs2: The entire query sequence</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
524 <option value="nmatch">nmatch: Match count</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
525 <option value="nmismatch">nmismatch: Mismatch count</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
526 <option value="ncolumn">ncolumn: Number of columns in the block. This includes matches, mismatches (substitutions), and gaps</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
527 <option value="npair">npair: Number of aligned bases in the block that are matches or mismatches (substitutions)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
528 <option value="ngap">ngap: Gap count, the number of gaps in the block, counting each run of gapped columns as a single gap</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
529 <option value="cgap">cgap: Gap column count, the number of gaps in the block, counting each gapped column as a separate gap</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
530 <option value="diff">diff: Differences between what would be written for text1 and text2</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
531 <option value="cigar">cigar: A CIGAR-like representation of the alignment’s path</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
532 <option value="cigarx">cigarx: Same as cigar, but uses a newer syntax that distinguishes matches from substitutions</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
533 <option value="identity" selected="true">identity: Fraction of aligned bases in the block that are matches </option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
534 <option value="idfrac">idfrac: Fraction of aligned bases in the block that are matches </option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
535 <option value="id%" selected="true">id% Fraction of aligned bases in the block that are matches (as %)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
536 <option value="blastid%">blastid%: Fraction of the alignment block that is matches, as would be reported by NCBI BLAST</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
537 <option value="continuity">continuity: Rate of non-gaps (non-indels) in the alignment block</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
538 <option value="confrac">confrac: Rate of non-gaps (non-indels) in the alignment block (as fraction)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
539 <option value="con%">con%: Rate of non-gaps (non-indels) in the alignment block (as %)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
540 <option value="coverage" selected="true">coverage: Fraction of the entire input sequence (target or query, whichever is shorter) that is covered by the alignment block</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
541 <option value="covfrac">covfrac: Fraction of the entire input sequence (target or query, whichever is shorter) that is covered by the alignment block (as fraction)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
542 <option value="cov%" selected="true">cov%: Fraction of the entire input sequence (target or query, whichever is shorter) that is covered by the alignment block (as %)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
543 <option value="diagonal">diagonal: The diagonal of the start of the alignment block in the DP matrix, expressed as an identifying number start1-start2</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
544 <option value="shingle">shingle: A measurement of the shingle overlap between the target and the query</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
545 <option value="number">number: The alignment number, counted as alignments are written to output (1-base)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
546 <option value="znumber">znumber: The alignment number, counted as alignments are written to output (0-base)</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
547 <sanitizer invalid_char="">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
548 <valid initial="string.letters,string.digits"><add value="%" /> </valid>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
549 </sanitizer>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
550 </param>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
551 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
552 <when value="maf">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
553 <param name="maf_type" type="select" display="radio" argument="--format=maf" label="Seleat MAF flavor" help="MAF is a multiple alignment format developed at UCSC">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
554 <option value="maf">MAF</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
555 <option value="maf+">MAF with additional stats</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
556 <option value="maf-">MAF without header and comments</option>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
557 </param>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
558 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
559 <when value="blastn">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
560 <!-- Do nothing -->
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
561 </when>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
562 </conditional>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
563 </section>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
564 </inputs>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
565 <outputs>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
566 <data format="tabular" name="output" label="${tool.name} on ${on_string}: mapped reads">
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
567 <change_format>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
568 <when input="output_format.out.format" value="bam" format="bam" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
569 <when input="output_format.out.format" value="maf" format="maf" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
570 </change_format>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
571 </data>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
572 <data format="png" name="out_plot" label="${tool.name} on ${on_string}: dot plot"/>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
573 </outputs>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
574 <tests>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
575 <test>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
576 <param name="ref_source" value="cached" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
577 <param name="target_2bit" value="phiX174" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
578 <param name="query" value="phiX_split.fasta" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
579 <param name="strand" value="--strand=both" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
580 <param name="format" value="general_def" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
581 <output name="output" value="test1.out" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
582 </test>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
583 <test>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
584 <param name="ref_source" value="history" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
585 <param name="target" value="phiX_split.fasta" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
586 <param name="query" value="phiX.fasta" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
587 <param name="strand" value="--strand=both" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
588 <param name="format" value="general_def" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
589 <output name="output" value="test2.out" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
590 </test>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
591 <test>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
592 <param name="ref_source" value="history" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
593 <param name="target" value="phiX_split.fasta" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
594 <param name="query" value="phiX.fasta" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
595 <param name="strand" value="--strand=both" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
596 <param name="score_file" value="score_file.txt" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
597 <param name="format" value="general_def" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
598 <output name="output" value="test3.out" />
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
599 </test>
7
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
600 <test>
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
601 <param name="ref_source" value="history" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
602 <param name="target" value="chrM_mouse.fa.gz" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
603 <param name="query" value="chrM_human.fa.gz" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
604 <param name="strand" value="--strand=both" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
605 <param name="format" value="blastn" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
606 <output name="output" value="test4.out" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
607 </test>
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
608 <test>
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
609 <param name="ref_source" value="history" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
610 <param name="target" ftype="fasta.gz" value="chrM_human.fa.gz" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
611 <param name="query" ftype="fastq.bz2" value="chrM_mouse.fq.bz2" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
612 <param name="strand" value="--strand=both" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
613 <param name="format" value="blastn" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
614 <output name="output" value="test5.out" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
615 </test>
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
616 <test>
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
617 <param name="ref_source" value="history" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
618 <param name="target" ftype="fasta.gz" value="chrM_human.fa.gz" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
619 <param name="query" ftype="fastq.gz" value="chrM_mouse.fq.gz" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
620 <param name="strand" value="--strand=both" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
621 <param name="format" value="blastn" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
622 <output name="output" value="test5.out" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
623 </test>
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
624 <test>
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
625 <param name="ref_source" value="history" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
626 <param name="target" ftype="fasta.gz" value="chrM_human.fa.gz" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
627 <param name="query" ftype="fastq" value="chrM_mouse.fq" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
628 <param name="strand" value="--strand=both" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
629 <param name="format" value="blastn" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
630 <output name="output" value="test5.out" />
10aca14c2332 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit 13e9724b44888b0de9535ac7b561ad9686038413
iuc
parents: 6
diff changeset
631 </test>
5
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
632 </tests>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
633
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
634 <help><![CDATA[
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
635
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
636 **What is does**
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
637
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
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diff changeset
638 LASTZ is designed to preprocess one sequence or set of sequences (which we collectively call the *TARGET*) and then align several *QUERY* sequences to it. It was developed by `Bob Harris <http://www.bx.psu.edu/~rsharris/>`_ in the lab of Webb Miller at Penn State.
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
639
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
640 .. class:: warningmark
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
641
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
642 **Read documentation** before proceeding. LASTZ is a complex tool with many parameter options. Fortunately, there is a `great manual <https://lastz.github.io/lastz/>`_ maintained by its author. Default parameters may be sufficient to obtain the initial idea about how similar your sequences are, but to produce reliable alignments you may need to tweak the parameters. So RTFM!
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
643
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
644 **About LASTZ parameters**
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
645
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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diff changeset
646 Galaxy's version of LASTZ has nine parameter sections (*Where to look*, *Scoring*, *Seeding*, *HSPs*, *Chaining*, *Gapped extension*, *Filtering*, *Interpolation*, and *Output*). These sections closely follow parameter description in the `manual <https://lastz.github.io/lastz/#syntax>`_.
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
647
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
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diff changeset
648 **Defaults**
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
649
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
650 here are defaults for some of the most important parameters::
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
651
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
652 --seed=<pattern> set seed pattern (12of19, 14of22, or general pattern)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
653 (default is 1110100110010101111)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
654 SEE "Seeding" SECTION -> "Select seed type"
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
655
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
656 --[no]transition allow (or don't) one transition in a seed hit
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
657 (by default a transition is allowed)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
658 SEE "Seeding" SECTION -> "Allow transitions"
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
659
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
660 --[no]chain perform chaining
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
661 (by default no chaining is performed)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
662 SEE "Chaining" SECTION
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
663
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
664 --[no]gapped perform gapped alignment (instead of gap-free)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
665 (by default gapped alignment is performed)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
666 SEE "Gapped extension" SECTION
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
667
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
668 --strand=both search both strands
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
669 --strand=plus search + strand only (matching strand of query spec)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
670 (by default both strands are searched)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
671 SEE "Where to look" SECTION
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
672
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
673 --scores=<file> read substitution and gap scores from a file
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
674 SEE "Scoring" SECTION
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
675
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
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diff changeset
676 --xdrop=<score> set x-drop threshold (default is 10sub[A][A])
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
677 SEE "HSPs" SECTION
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
678
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
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679 --ydrop=<score> set y-drop threshold (default is open+300extend)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
680 SEE "Gapped extension" SECTION
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
681
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
682 --hspthresh=<score> set threshold for high scoring pairs (default is 3000)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
683 ungapped extensions scoring lower are discarded
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
684 <score> can also be a percentage or base count
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
685 SEE "HSPs" SECTION
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
686
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
687 --gappedthresh=<score> set threshold for gapped alignments
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
688 gapped extensions scoring lower are discarded
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
689 <score> can also be a percentage or base count
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
690 (default is to use same value as --hspthresh)
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
691 SEE "Gapped extension" SECTION
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
692
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
693
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
694 **Substitution matrix**
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
695
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
696 By default the HOXD70 substitution scores are used (from `Chiaromonte et al. 2002 <https://www.ncbi.nlm.nih.gov/pubmed/11928468>`_)::
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
697
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
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698 bad_score = X:-1000 # used for sub['X'][*] and sub[*]['X']
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
699 fill_score = -100 # used when sub[*][*] is not defined
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
700 gap_open_penalty = 400
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
diff changeset
701 gap_extend_penalty = 30
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
devteam
parents:
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702
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703 A C G T
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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704 A 91 -114 -31 -123
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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705 C -114 100 -125 -31
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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706 G -31 -125 100 -114
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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707 T -123 -31 -114 91
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708
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709 Matrix can be supplied as an input to **Read the substitution scores** parameter in *Scoring* section. Substitution matrix can be inferred from your data using another LASTZ-based tool (LASTZ_D: Infer substitution scores).
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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710
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711 **Output**
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712
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713 This version of LASTZ produces two outputs by default: a BAM alignment file and a dot-plot in PNG format. Other formats can be configured in *Output* section. This incarnation of LASTZ produces outputs without comment line starting with '#'. To learn identity of each column, consult `formats section of LASTZ manual <https://lastz.github.io/lastz/#formats>`_.
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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714
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715 ]]>
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716 </help>
bd84ff27bc16 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/lastz commit a0a0480a8df511d23ed6101a489ca06337f5ed56
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717 <expand macro="citations"/>
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718 </tool>