annotate tools/ncbi_blast_plus/ncbi_macros.xml @ 19:7f3c448e119b draft

v0.1.07 - fix macro problem in blastxml_to_tabular.xml, reenable .loc tests
author peterjc
date Thu, 19 Nov 2015 06:30:35 -0500
parents 577d9c12411a
children 3034ce97dd33
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1 <macros>
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7f3c448e119b v0.1.07 - fix macro problem in blastxml_to_tabular.xml, reenable .loc tests
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2 <token name="@WRAPPER_VERSION@">0.1.07</token>
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3 <xml name="parallelism">
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4 <!-- If job splitting is enabled, break up the query file into parts -->
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5 <parallelism method="multi" split_inputs="query" split_mode="to_size" split_size="1000" merge_outputs="output1" />
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6 </xml>
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7 <xml name="preamble">
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8 <requirements>
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9 <requirement type="binary">@BINARY@</requirement>
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577d9c12411a v0.1.06, now using BLAST+ 2.2.31
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10 <requirement type="package" version="2.2.31">blast+</requirement>
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11 </requirements>
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12 <stdio>
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13 <!-- Anything other than zero is an error -->
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14 <exit_code range="1:" />
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15 <!-- Might see negative return codes for Unix signals via Python subprocess -->
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16 <exit_code range=":-1" />
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17 <!-- In case the return code has not been set properly check stderr too -->
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18 <regex match="Error:" />
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19 <regex match="Exception:" />
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20 </stdio>
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21 <version_command>@BINARY@ -version</version_command>
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22 </xml>
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23 <xml name="output_change_format">
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24 <change_format>
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25 <when input="output.out_format" value="0" format="txt"/>
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26 <when input="output.out_format" value="0 -html" format="html"/>
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27 <when input="output.out_format" value="2" format="txt"/>
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28 <when input="output.out_format" value="2 -html" format="html"/>
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29 <when input="output.out_format" value="4" format="txt"/>
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30 <when input="output.out_format" value="4 -html" format="html"/>
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31 <when input="output.out_format" value="5" format="blastxml"/>
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32 </change_format>
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33 </xml>
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34 <xml name="input_out_format">
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35 <conditional name="output">
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36 <param name="out_format" type="select" label="Output format">
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37 <option value="6">Tabular (standard 12 columns)</option>
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38 <option value="ext" selected="True">Tabular (extended 25 columns)</option>
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39 <option value="cols">Tabular (select which columns)</option>
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40 <option value="5">BLAST XML</option>
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41 <option value="0">Pairwise text</option>
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42 <option value="0 -html">Pairwise HTML</option>
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43 <option value="2">Query-anchored text</option>
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44 <option value="2 -html">Query-anchored HTML</option>
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45 <option value="4">Flat query-anchored text</option>
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46 <option value="4 -html">Flat query-anchored HTML</option>
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47 <!--
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48 <option value="-outfmt 11">BLAST archive format (ASN.1)</option>
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49 -->
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50 </param>
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51 <when value="6"/>
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52 <when value="ext"/>
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53 <when value="cols">
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54 <param name="std_cols" type="select" multiple="true" display="checkboxes" label="Standard columns">
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55 <option selected="true" value="qseqid">qseqid = Query Seq-id (ID of your sequence)</option>
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56 <option selected="true" value="sseqid">sseqid = Subject Seq-id (ID of the database hit)</option>
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57 <option selected="true" value="pident">pident = Percentage of identical matches</option>
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58 <option selected="true" value="length">length = Alignment length</option>
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59 <option selected="true" value="mismatch">mismatch = Number of mismatches</option>
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60 <option selected="true" value="gapopen">gapopen = Number of gap openings</option>
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61 <option selected="true" value="qstart">qstart = Start of alignment in query</option>
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62 <option selected="true" value="qend">qend = End of alignment in query</option>
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63 <option selected="true" value="sstart">sstart = Start of alignment in subject (database hit)</option>
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64 <option selected="true" value="send">send = End of alignment in subject (database hit)</option>
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65 <option selected="true" value="evalue">evalue = Expectation value (E-value)</option>
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66 <option selected="true" value="bitscore">bitscore = Bit score</option>
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67 </param>
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68 <param name="ext_cols" type="select" multiple="true" display="checkboxes" label="Extended columns">
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69 <option value="sallseqid">sallseqid = All subject Seq-id(s), separated by a ';'</option>
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70 <option value="score">score = Raw score</option>
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71 <option value="nident">nident = Number of identical matches</option>
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72 <option value="positive">positive = Number of positive-scoring matches</option>
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73 <option value="gaps">gaps = Total number of gaps</option>
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74 <option value="ppos">ppos = Percentage of positive-scoring matches</option>
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75 <option value="qframe">qframe = Query frame</option>
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76 <option value="sframe">sframe = Subject frame</option>
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77 <option value="qseq">qseq = Aligned part of query sequence</option>
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78 <option value="sseq">sseq = Aligned part of subject sequence</option>
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79 <option value="qlen">qlen = Query sequence length</option>
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80 <option value="slen">slen = Subject sequence length</option>
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81 <option value="salltitles">salltitles = All subject title(s), separated by a '&lt;&gt;'</option>
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82 </param>
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83 <param name="ids_cols" type="select" multiple="true" display="checkboxes" label="Other identifier columns">
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84 <option value="qgi">qgi = Query GI</option>
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85 <option value="qacc">qacc = Query accesion</option>
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86 <option value="qaccver">qaccver = Query accesion.version</option>
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87 <option value="sallseqid">sallseqid = All subject Seq-id(s), separated by a ';'</option>
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88 <option value="sgi">sgi = Subject GI</option>
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89 <option value="sallgi">sallgi = All subject GIs</option>
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90 <option value="sacc">sacc = Subject accession</option>
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91 <option value="saccver">saccver = Subject accession.version</option>
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92 <option value="sallacc">sallacc = All subject accessions</option>
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93 <option value="stitle">stitle = Subject Title</option>
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94 </param>
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95 <param name="misc_cols" type="select" multiple="true" display="checkboxes" label="Miscellaneous columns">
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96 <option value="sstrand">sstrand = Subject Strand</option>
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97 <!-- Is it really worth including 'frames' given have 'qframe' and 'sframe'? -->
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98 <option value="frames">frames = Query and subject frames separated by a '/'</option>
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99 <option value="btop">btop = Blast traceback operations (BTOP)</option>
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100 <option value="qcovs">qcovs = Query Coverage Per Subject</option>
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101 <option value="qcovhsp">qcovhsp = Query Coverage Per HSP</option>
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102 </param>
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103 <param name="tax_cols" type="select" multiple="true" display="checkboxes" label="Taxonomy columns">
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104 <option value="staxids">staxids = unique Subject Taxonomy ID(s), separated by a ';' (in numerical order)</option>
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105 <!-- TODO, how to handle the taxonomy data file dependency? If missing these give N/A -->
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106 <option value="sscinames">sscinames = unique Subject Scientific Name(s), separated by a ';'</option>
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107 <option value="scomnames">scomnames = unique Subject Common Name(s), separated by a ';'</option>
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108 <option value="sblastnames">sblastnames = unique Subject Blast Name(s), separated by a ';' (in alphabetical order)</option>
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109 <option value="sskingdoms">sskingdoms = unique Subject Super Kingdom(s), separated by a ';' (in alphabetical order)</option>
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110 </param>
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111 </when>
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112 <when value="5"/>
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113 <when value="0"/>
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114 <when value="0 -html"/>
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115 <when value="2"/>
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116 <when value="2 -html"/>
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117 <when value="4"/>
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118 <when value="4 -html"/>
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119 </conditional>
11
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120 </xml>
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121 <xml name="input_scoring_matrix">
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122 <param name="matrix" type="select" label="Scoring matrix">
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123 <option value="BLOSUM90">BLOSUM90</option>
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124 <option value="BLOSUM80">BLOSUM80</option>
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125 <option value="BLOSUM62" selected="true">BLOSUM62 (default)</option>
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126 <option value="BLOSUM50">BLOSUM50</option>
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127 <option value="BLOSUM45">BLOSUM45</option>
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128 <option value="PAM250">PAM250</option>
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129 <option value="PAM70">PAM70</option>
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130 <option value="PAM30">PAM30</option>
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131 </param>
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132 </xml>
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133 <xml name="input_query_gencode">
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134 <param name="query_gencode" type="select" label="Query genetic code">
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135 <!-- See http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi for details -->
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136 <option value="1" select="True">1. Standard</option>
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137 <option value="2">2. Vertebrate Mitochondrial</option>
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138 <option value="3">3. Yeast Mitochondrial</option>
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139 <option value="4">4. Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option>
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140 <option value="5">5. Invertebrate Mitochondrial</option>
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141 <option value="6">6. Ciliate, Dasycladacean and Hexamita Nuclear Code</option>
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142 <option value="9">9. Echinoderm Mitochondrial</option>
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143 <option value="10">10. Euplotid Nuclear</option>
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144 <option value="11">11. Bacteria and Archaea</option>
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145 <option value="12">12. Alternative Yeast Nuclear</option>
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146 <option value="13">13. Ascidian Mitochondrial</option>
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147 <option value="14">14. Flatworm Mitochondrial</option>
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148 <option value="15">15. Blepharisma Macronuclear</option>
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149 <option value="16">16. Chlorophycean Mitochondrial Code</option>
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150 <option value="21">21. Trematode Mitochondrial Code</option>
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151 <option value="22">22. Scenedesmus obliquus mitochondrial Code</option>
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152 <option value="23">23. Thraustochytrium Mitochondrial Code</option>
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153 <option value="24">24. Pterobranchia mitochondrial code</option>
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154 </param>
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155 </xml>
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156 <xml name="input_db_gencode">
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157 <param name="db_gencode" type="select" label="Database/subject genetic code">
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158 <!-- See http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi for details -->
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159 <option value="1" select="True">1. Standard</option>
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160 <option value="2">2. Vertebrate Mitochondrial</option>
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161 <option value="3">3. Yeast Mitochondrial</option>
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162 <option value="4">4. Mold, Protozoan, and Coelenterate Mitochondrial Code and the Mycoplasma/Spiroplasma Code</option>
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163 <option value="5">5. Invertebrate Mitochondrial</option>
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164 <option value="6">6. Ciliate, Dasycladacean and Hexamita Nuclear Code</option>
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165 <option value="9">9. Echinoderm Mitochondrial</option>
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166 <option value="10">10. Euplotid Nuclear</option>
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167 <option value="11">11. Bacteria and Archaea</option>
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168 <option value="12">12. Alternative Yeast Nuclear</option>
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169 <option value="13">13. Ascidian Mitochondrial</option>
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170 <option value="14">14. Flatworm Mitochondrial</option>
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171 <option value="15">15. Blepharisma Macronuclear</option>
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172 <option value="16">16. Chlorophycean Mitochondrial Code</option>
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173 <option value="21">21. Trematode Mitochondrial Code</option>
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174 <option value="22">22. Scenedesmus obliquus mitochondrial Code</option>
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175 <option value="23">23. Thraustochytrium Mitochondrial Code</option>
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176 <option value="24">24. Pterobranchia mitochondrial code</option>
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177 </param>
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178 </xml>
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179 <xml name="input_conditional_nucleotide_db">
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180 <conditional name="db_opts">
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181 <param name="db_opts_selector" type="select" label="Subject database/sequences">
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182 <option value="db" selected="True">Locally installed BLAST database</option>
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183 <option value="histdb">BLAST database from your history</option>
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184 <option value="file">FASTA file from your history (see warning note below)</option>
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185 </param>
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186 <when value="db">
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187 <param name="database" type="select" label="Nucleotide BLAST database">
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188 <options from_data_table="blastdb" />
11
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189 </param>
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190 <param name="histdb" type="hidden" value="" />
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191 <param name="subject" type="hidden" value="" />
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192 </when>
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193 <when value="histdb">
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194 <param name="database" type="hidden" value="" />
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195 <param name="histdb" type="data" format="blastdbn" label="Nucleotide BLAST database" />
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196 <param name="subject" type="hidden" value="" />
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197 </when>
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198 <when value="file">
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199 <param name="database" type="hidden" value="" />
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200 <param name="histdb" type="hidden" value="" />
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201 <param name="subject" type="data" format="fasta" label="Nucleotide FASTA file to use as database"/>
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202 </when>
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203 </conditional>
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204 </xml>
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205 <xml name="input_conditional_protein_db">
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206 <conditional name="db_opts">
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207 <param name="db_opts_selector" type="select" label="Subject database/sequences">
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208 <option value="db" selected="True">Locally installed BLAST database</option>
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209 <option value="histdb">BLAST database from your history</option>
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210 <option value="file">FASTA file from your history (see warning note below)</option>
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211 </param>
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212 <when value="db">
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213 <param name="database" type="select" label="Protein BLAST database">
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214 <options from_data_table="blastdb_p" />
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215 </param>
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216 <param name="histdb" type="hidden" value="" />
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217 <param name="subject" type="hidden" value="" />
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218 </when>
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219 <when value="histdb">
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220 <param name="database" type="hidden" value="" />
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221 <param name="histdb" type="data" format="blastdbp" label="Protein BLAST database" />
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222 <param name="subject" type="hidden" value="" />
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223 </when>
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224 <when value="file">
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225 <param name="database" type="hidden" value="" />
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226 <param name="histdb" type="hidden" value="" />
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227 <param name="subject" type="data" format="fasta" label="Protein FASTA file to use as database"/>
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228 </when>
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229 </conditional>
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230 </xml>
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231 <xml name="input_conditional_pssm">
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232 <conditional name="db_opts">
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233 <param name="db_opts_selector" type="select" label="Protein domain database (PSSM)">
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234 <option value="db" selected="True">Locally installed BLAST protein domain database</option>
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235 <option value="histdb">BLAST protein domain database from your history</option>
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236 </param>
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237 <when value="db">
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238 <param name="database" type="select" label="Protein domain database">
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239 <options from_data_table="blastdb_d" />
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240 </param>
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241 <param name="histdb" type="hidden" value="" />
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242 <param name="subject" type="hidden" value="" />
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243 </when>
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244 <when value="histdb">
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245 <param name="database" type="hidden" value="" />
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246 <param name="histdb" type="data" format="blastdbd" label="Protein domain database" />
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247 <param name="subject" type="hidden" value="" />
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248 </when>
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249 </conditional>
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250 </xml>
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251 <xml name="input_conditional_choose_db_type">
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252 <conditional name="db_opts">
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253 <param name="db_type" type="select" label="Type of BLAST database">
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254 <option value="nucl" selected="True">Nucleotide</option>
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255 <option value="prot">Protein</option>
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256 </param>
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257 <when value="nucl">
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258 <param name="database" type="select" label="Nucleotide BLAST database">
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259 <options from_data_table="blastdb" />
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260 </param>
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261 </when>
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262 <when value="prot">
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263 <param name="database" type="select" label="Protein BLAST database">
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264 <options from_data_table="blastdb_p" />
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265 </param>
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266 </when>
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267 </conditional>
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268 </xml>
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269 <xml name="input_parse_deflines">
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270 <param name="parse_deflines" type="boolean" label="Should the query and subject defline(s) be parsed?" truevalue="-parse_deflines" falsevalue="" checked="false" help="This affects the formatting of the query/subject ID strings"/>
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271 </xml>
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272 <xml name="input_filter_query_default_false">
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273 <param name="filter_query" type="boolean" label="Filter out low complexity regions (with SEG)" truevalue="-seg yes" falsevalue="-seg no" checked="false" />
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274 </xml>
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275 <xml name="input_filter_query_default_true">
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276 <param name="filter_query" type="boolean" label="Filter out low complexity regions (with SEG)" truevalue="-seg yes" falsevalue="-seg no" checked="true" />
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277 </xml>
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278 <xml name="input_max_hits">
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279 <param name="max_hits" type="integer" value="0" label="Maximum hits to show" help="Use zero for default limits">
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280 <validator type="in_range" min="0" />
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281 </param>
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282 </xml>
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283 <xml name="input_evalue">
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284 <param name="evalue_cutoff" type="float" size="15" value="0.001" label="Set expectation value cutoff" />
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285 </xml>
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286 <xml name="input_word_size">
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287 <param name="word_size" type="integer" value="0" label="Word size for wordfinder algorithm" help="Use zero for default, otherwise minimum 2.">
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288 <validator type="in_range" min="0" />
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289 </param>
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290 </xml>
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291 <xml name="input_strand">
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292 <param name="strand" type="select" label="Query strand(s) to search against database/subject">
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293 <option value="-strand both">Both</option>
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294 <option value="-strand plus">Plus (forward)</option>
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295 <option value="-strand minus">Minus (reverse complement)</option>
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296 </param>
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297 </xml>
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298 <xml name="input_qcov_hsp_perc">
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299 <param name="qcov_hsp_perc" type="float" value="0" min="0" max="100" label="Minimum query coverage per hsp (percentage, 0 to 100)" help="See also the output column qhspcov"/>
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300 </xml>
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301 <xml name="advanced_options">
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302 <conditional name="adv_opts">
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303 <param name="adv_opts_selector" type="select" label="Advanced Options">
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304 <option value="basic" selected="True">Hide Advanced Options</option>
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305 <option value="advanced">Show Advanced Options</option>
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306 </param>
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307 <when value="basic" />
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308 <when value="advanced">
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309 <yield />
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310 </when>
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311 </conditional>
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312 </xml>
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313 <xml name="advanced_optional_id_files">
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314 <conditional name="adv_optional_id_files_opts">
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315 <param name="adv_optional_id_files_opts_selector" type="select"
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316 label="Restrict search of database to a given set of ID's"
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317 help="This feature provides a means to exclude ID's from a BLAST database search. The expectation values in the BLAST results are based upon the sequences actually searched, and not on the underlying database. Note this cannot be used when comparing against a FASTA file.">
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318 <option value="none" selected="True">No restriction, search the entire database</option>
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319 <option value="gilist">GI identifiers</option>
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320 <option value="negative_gilist">Negative GI identifiers</option>
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321 <option value="seqidlist">Sequence identifiers (SeqId's)</option>
14
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322 </param>
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parents: 13
diff changeset
323 <when value="none" />
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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parents: 13
diff changeset
324 <when value="gilist">
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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325 <param name="gilist" type="data" format="txt" label="Restrict search of database to GI's listed in this file"
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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326 help="This option is only available for database searches."/>
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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327 </when>
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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parents: 13
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328 <when value="negative_gilist">
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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329 <param name="negative_gilist" type="data" format="txt" label="Restrict search of database to everything except the GI's listed in this file"
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
parents: 13
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330 help="This option is only available for database searches."/>
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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parents: 13
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331 </when>
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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parents: 13
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332 <when value="seqidlist">
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
peterjc
parents: 13
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333 <param name="seqidlist" type="data" format="txt" label=" Restrict search of database to list of SeqId's"
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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parents: 13
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334 help="This option is only available for database searches."/>
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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335 </when>
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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336 </conditional>
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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337 </xml>
15
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338 <token name="@ADV_QCOV_HSP_PERC@">
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339 #if float(str($adv_opts.qcov_hsp_perc)) &gt; 0:
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340 -qcov_hsp_perc $adv_opts.qcov_hsp_perc
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341 #end if
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342 </token>
14
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343 <token name="@ADV_ID_LIST_FILTER@">
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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344 #if $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'negative_gilist':
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345 -negative_gilist $adv_opts.adv_optional_id_files_opts.negative_gilist
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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346 #elif $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'gilist':
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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347 -gilist $adv_opts.adv_optional_id_files_opts.gilist
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348 #elif $adv_opts.adv_optional_id_files_opts.adv_optional_id_files_opts_selector == 'seqidlist':
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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349 -seqidlist $adv_opts.adv_optional_id_files_opts.seqidlist
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350 #end if
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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351 </token>
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352 <token name="@THREADS@">-num_threads "\${GALAXY_SLOTS:-8}"</token>
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353 <token name="@BLAST_DB_SUBJECT@">
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354 #if $db_opts.db_opts_selector == "db":
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355 -db "${db_opts.database.fields.path}"
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356 #elif $db_opts.db_opts_selector == "histdb":
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357 -db "${os.path.join($db_opts.histdb.extra_files_path,'blastdb')}"
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358 #else:
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359 -subject "$db_opts.subject"
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360 #end if
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361 </token>
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362 <token name="@BLAST_OUTPUT@">-out "$output1"
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363 ##Set the extended list here so when we add things, saved workflows are not affected
13
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364 #if str($output.out_format)=="ext":
11
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365 -outfmt "6 std sallseqid score nident positive gaps ppos qframe sframe qseq sseq qlen slen salltitles"
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366 #elif str($output.out_format)=="cols"
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diff changeset
367 ##Pick your own columns. Galaxy gives us it comma separated, BLAST+ wants space separated:
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diff changeset
368 ##TODO - Can we catch the user picking no columns and raise an error here?
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369 #set cols = (str($output.std_cols)+","+str($output.ext_cols)+","+str($output.ids_cols)+","+str($output.misc_cols)+","+str($output.tax_cols)).replace("None", "").replace(",,", ",").replace(",", " ").strip()
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370 -outfmt "6 $cols"
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371 #else:
13
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372 -outfmt $output.out_format
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373 #end if
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374 </token>
15
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375 <token name="@ADV_FILTER_QUERY@">$adv_opts.filter_query</token>
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376 <token name="@ADV_MAX_HITS@">
11
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377 ## Need int(str(...)) because $adv_opts.max_hits is an InputValueWrapper object not a string
15
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378 ## Note -max_target_seqs used to simply override -num_descriptions and -num_alignments
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diff changeset
379 ## but this was changed in BLAST+ 2.2.27 onwards to force their use (raised with NCBI)
11
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380 #if (str($adv_opts.max_hits) and int(str($adv_opts.max_hits)) > 0):
15
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381 #if str($output.out_format) in ["6", "ext", "cols", "5"]:
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diff changeset
382 ## Most output formats use this, including tabular and XML:
11
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383 -max_target_seqs $adv_opts.max_hits
15
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384 #else
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diff changeset
385 ## Text and HTML output formats 0-4 currently need this instead:
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386 -num_descriptions $adv_opts.max_hits -num_alignments $adv_opts.max_hits
11
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387 #end if
15
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388 #end if
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389 </token>
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diff changeset
390 <token name="@ADV_WORD_SIZE@">
11
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391 #if (str($adv_opts.word_size) and int(str($adv_opts.word_size)) > 0):
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392 -word_size $adv_opts.word_size
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393 #end if
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394 $adv_opts.parse_deflines
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395 </token>
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parents:
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396 <!-- @ON_DB_SUBJECT@ is for use with @BLAST_DB_SUBJECT@ -->
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parents:
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397 <token name="@ON_DB_SUBJECT@">#if str($db_opts.db_opts_selector)=='db'
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parents:
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398 ${db_opts.database}
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parents:
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399 #elif str($db_opts.db_opts_selector)=='histdb'
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parents:
diff changeset
400 ${db_opts.histdb.name}
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parents:
diff changeset
401 #else
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peterjc
parents:
diff changeset
402 ${db_opts.subject.name}
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parents:
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403 #end if</token>
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parents:
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404 <token name="@REFERENCES@">
15
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diff changeset
405 Peter J. A. Cock, John M. Chilton, Björn Grüning, James E. Johnson, Nicola Soranzo (2015).
17
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diff changeset
406 NCBI BLAST+ integrated into Galaxy. *GigaScience* 4:39
697f40151eaf v0.1.05 - Update citation information now GigaScience paper is out
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parents: 16
diff changeset
407 http://dx.doi.org/10.1186/s13742-015-0080-7
11
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408
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parents:
diff changeset
409 Christiam Camacho et al. (2009).
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parents:
diff changeset
410 BLAST+: architecture and applications.
17
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parents: 16
diff changeset
411 *BMC Bioinformatics* 15;10:421.
11
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parents:
diff changeset
412 http://dx.doi.org/10.1186/1471-2105-10-421
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413
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parents:
diff changeset
414 This wrapper is available to install into other Galaxy Instances via the Galaxy
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parents:
diff changeset
415 Tool Shed at http://toolshed.g2.bx.psu.edu/view/devteam/ncbi_blast_plus
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416 </token>
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2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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417 <xml name="blast_citations">
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418 <citations>
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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419 <citation type="doi">10.1186/1471-2105-10-421</citation>
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697f40151eaf v0.1.05 - Update citation information now GigaScience paper is out
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420 <citation type="doi">10.1186/s13742-015-0080-7</citation>
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421 </citations>
2fe07f50a41e Uploaded v0.1.01 - Requires blastdbd datatype (blast_datatypes v0.0.19). Support for makeprofiledb to create protein domain databases and use them in RPS-BLAST and RPS-TBLASTN. Tools now support GI and SeqID filters, and embed the citations.
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422 </xml>
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423 <token name="@OUTPUT_FORMAT@">**Output format**
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424
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425 Because Galaxy focuses on processing tabular data, the default output of this
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426 tool is tabular. The standard BLAST+ tabular output contains 12 columns:
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427
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428 ====== ========= ============================================
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429 Column NCBI name Description
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430 ------ --------- --------------------------------------------
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431 1 qseqid Query Seq-id (ID of your sequence)
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432 2 sseqid Subject Seq-id (ID of the database hit)
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433 3 pident Percentage of identical matches
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434 4 length Alignment length
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435 5 mismatch Number of mismatches
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436 6 gapopen Number of gap openings
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437 7 qstart Start of alignment in query
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438 8 qend End of alignment in query
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439 9 sstart Start of alignment in subject (database hit)
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440 10 send End of alignment in subject (database hit)
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441 11 evalue Expectation value (E-value)
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442 12 bitscore Bit score
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443 ====== ========= ============================================
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444
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445 The BLAST+ tools can optionally output additional columns of information,
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446 but this takes longer to calculate. Many commonly used extra columns are
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447 included by selecting the extended tabular output. The extra columns are
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448 included *after* the standard 12 columns. This is so that you can write
4c4a0da938ff Uploaded v0.0.22, now wraps BLAST+ 2.2.28 allowing extended tabular output to include the hit descriptions as column 25.
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449 workflow filtering steps that accept either the 12 or 25 column tabular
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450 BLAST output. Galaxy now uses this extended 25 column output by default.
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451
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452 ====== ============= ===========================================
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453 Column NCBI name Description
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454 ------ ------------- -------------------------------------------
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455 13 sallseqid All subject Seq-id(s), separated by a ';'
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456 14 score Raw score
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457 15 nident Number of identical matches
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458 16 positive Number of positive-scoring matches
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459 17 gaps Total number of gaps
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460 18 ppos Percentage of positive-scoring matches
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461 19 qframe Query frame
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462 20 sframe Subject frame
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463 21 qseq Aligned part of query sequence
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464 22 sseq Aligned part of subject sequence
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465 23 qlen Query sequence length
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466 24 slen Subject sequence length
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467 25 salltitles All subject title(s), separated by a '&lt;&gt;'
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468 ====== ============= ===========================================
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469
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470 The third option is to customise the tabular output by selecting which
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471 columns you want, from the standard set of 12, the default set of 25,
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472 or any of the additional columns BLAST+ offers (including species name).
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473
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474 The fourth option is BLAST XML output, which is designed to be parsed by
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475 another program, and is understood by some Galaxy tools.
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476
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477 You can also choose several plain text or HTML output formats which are designed to be read by a person (not by another program).
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478 The HTML versions use basic webpage formatting and can include links to the hits on the NCBI website.
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479 The pairwise output (the default on the NCBI BLAST website) shows each match as a pairwise alignment with the query.
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480 The two query anchored outputs show a multiple sequence alignment between the query and all the matches,
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481 and differ in how insertions are shown (marked as insertions or with gap characters added to the other sequences).
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482 </token>
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483 <token name="@FASTA_WARNING@">.. class:: warningmark
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484
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485 You can also search against a FASTA file of subject (target)
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486 sequences. This is *not* advised because it is slower (only one
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487 CPU is used), but more importantly gives e-values for pairwise
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488 searches (very small e-values which will look overly signficiant).
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489 In most cases you should instead turn the other FASTA file into a
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490 database first using *makeblastdb* and search against that.
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491 </token>
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492 <token name="@SEARCH_TIME_WARNING@">.. class:: warningmark
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493
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494 **Note**. Database searches may take a substantial amount of time.
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495 For large input datasets it is advisable to allow overnight processing.
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496
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497 -----
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498 </token>
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499 </macros>