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author | peterjc |
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date | Thu, 22 Feb 2024 15:07:36 +0000 |
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<HTML> <HEAD><TITLE>BLAST Search Results</TITLE></HEAD> <BODY BGCOLOR="#FFFFFF" LINK="#0000FF" VLINK="#660099" ALINK="#660099"> <PRE> <b>TBLASTN 2.14.1+</b> <b><a href="https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=R etrieve&list_uids=9254694&dopt=Citation">Reference</a>:</b> Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: User specified sequence set (Input: rhodopsin_nucs.fasta). 6 sequences; 10,296 total letters <b>Query=</b> sp|Q9BS26|ERP44_HUMAN Endoplasmic reticulum resident protein 44 OS=Homo sapiens GN=ERP44 PE=1 SV=1 Length=406 ***** No hits found ***** Lambda K H a alpha 0.347 0.182 0.684 0.522 1.92 Gapped Lambda K H a alpha sigma 0.299 0.0710 0.270 1.10 13.8 14.5 Effective search space used: 1239330 <b>Query=</b> sp|Q9NSY1|BMP2K_HUMAN BMP-2-inducible protein kinase OS=Homo sapiens GN=BMP2K PE=1 SV=2 Length=1161 ***** No hits found ***** Lambda K H a alpha 0.334 0.170 0.615 0.522 1.92 Gapped Lambda K H a alpha sigma 0.299 0.0710 0.270 1.10 13.8 14.5 Effective search space used: 3667980 <b>Query=</b> sp|P06213|INSR_HUMAN Insulin receptor OS=Homo sapiens GN=INSR PE=1 SV=4 Length=1382 ***** No hits found ***** Lambda K H a alpha 0.346 0.180 0.700 0.522 1.92 Gapped Lambda K H a alpha sigma 0.299 0.0710 0.270 1.10 13.8 14.5 Effective search space used: 4374000 <b>Query=</b> sp|P08100|OPSD_HUMAN Rhodopsin OS=Homo sapiens GN=RHO PE=1 SV=1 Length=348 Score E Sequences producing significant alignments: (Bits) Value gi|57163782|ref|NM_001009242.1| Felis catus rhodopsin (RHO), mRNA <a href=#Subject_1>732</a> 0.0 gi|18148870|dbj|AB062417.1| Synthetic construct Bos taurus gene f... <a href=#Subject_5>711</a> 0.0 gi|283855822|gb|GQ290312.1| Myotis ricketti voucher GQX10 rhodops... <a href=#Subject_4>682</a> 0.0 gi|2734705|gb|U59921.1|BBU59921 Bufo bufo rhodopsin mRNA, complet... <a href=#Subject_2>646</a> 0.0 gi|12583664|dbj|AB043817.1| Conger myriaster conf gene for fresh ... <a href=#Subject_6>626</a> 0.0 gi|283855845|gb|GQ290303.1| Cynopterus brachyotis voucher 2002043... <a href=#Subject_3>229</a> 3e-67 ><a name=Subject_1></a> gi|57163782|ref|NM_001009242.1| Felis catus rhodopsin (RHO), mRNA Length=1047 Score = 732 bits (1689), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/348 (97%), Positives = 343/348 (99%), Gaps = 0/348 (0%) Frame = +1 Query 1 MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY 60 MNGTEGPNFYVPFSN TGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY Sbjct 1 MNGTEGPNFYVPFSNKTGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY 180 Query 61 VTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG 120 VTVQHKKLRTPLNYILLNLAVADLFMV GGFT+TLYTSLHGYFVFGPTGCNLEGFFATLG Sbjct 181 VTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLG 360 Query 121 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIP 180 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPL GWSRYIP Sbjct 361 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIP 540 Query 181 EGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQES 240 EG+QCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMI+IFFCYGQLVFTVKEAAAQQQES Sbjct 541 EGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIVIFFCYGQLVFTVKEAAAQQQES 720 Query 241 ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAI 300 ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMT+PAFFAKS++I Sbjct 721 ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTLPAFFAKSSSI 900 Query 301 YNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA 348 YNPVIYIMMNKQFRNCMLTT+CCGKNPLGDDEAS T SKTETSQVAPA Sbjct 901 YNPVIYIMMNKQFRNCMLTTLCCGKNPLGDDEASTTGSKTETSQVAPA 1044 ><a name=Subject_5></a> gi|18148870|dbj|AB062417.1| Synthetic construct Bos taurus gene for rhodopsin, complete cds Length=1047 Score = 711 bits (1640), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/348 (93%), Positives = 337/348 (97%), Gaps = 0/348 (0%) Frame = +1 Query 1 MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY 60 MNGTEGPNFYVPFSN TGVVRSPFE PQYYLAEPWQFSMLAAYMFLLI+LGFPINFLTLY Sbjct 1 MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIMLGFPINFLTLY 180 Query 61 VTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG 120 VTVQHKKLRTPLNYILLNLAVADLFMV GGFT+TLYTSLHGYFVFGPTGCNLEGFFATLG Sbjct 181 VTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLG 360 Query 121 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIP 180 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPL GWSRYIP Sbjct 361 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIP 540 Query 181 EGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQES 240 EG+QCSCGIDYYT E NNESFVIYMFVVHF IP+I+IFFCYGQLVFTVKEAAAQQQES Sbjct 541 EGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQES 720 Query 241 ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAI 300 ATTQKAEKEVTRMVIIMVIAFLICW+PYA VAFYIFTHQGS+FGPIFMTIPAFFAK++A+ Sbjct 721 ATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAV 900 Query 301 YNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA 348 YNPVIYIMMNKQFRNCM+TT+CCGKNPLGDDEAS TVSKTETSQVAPA Sbjct 901 YNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA 1044 ><a name=Subject_4></a> gi|283855822|gb|GQ290312.1| Myotis ricketti voucher GQX10 rhodopsin (RHO) mRNA, partial cds Length=983 Score = 682 bits (1573), Expect = 0.0, Method: Compositional matrix adjust. Identities = 310/326 (95%), Positives = 322/326 (99%), Gaps = 0/326 (0%) Frame = +1 Query 11 VPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT 70 VPFSN TGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT Sbjct 1 VPFSNKTGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT 180 Query 71 PLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVV 130 PLNYILLNLAVA+LFMV GGFT+TLYTS+HGYFVFG TGCNLEGFFATLGGEIALWSLVV Sbjct 181 PLNYILLNLAVANLFMVFGGFTTTLYTSMHGYFVFGATGCNLEGFFATLGGEIALWSLVV 360 Query 131 LAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPEGLQCSCGID 190 LAIERYVVVCKPMSNFRFGENHAIMG+AFTWVMALACAAPPLAGWSRYIPEG+QCSCGID Sbjct 361 LAIERYVVVCKPMSNFRFGENHAIMGLAFTWVMALACAAPPLAGWSRYIPEGMQCSCGID 540 Query 191 YYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQESATTQKAEKEV 250 YYTLKPEVNNESFVIYMFVVHFTIPMI+IFFCYGQLVFTVKEAAAQQQESATTQKAEKEV Sbjct 541 YYTLKPEVNNESFVIYMFVVHFTIPMIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEV 720 Query 251 TRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMN 310 TRMVIIMV+AFLICW+PYASVAFYIFTHQGSNFGP+FMTIPAFFAKS++IYNPVIYIMMN Sbjct 721 TRMVIIMVVAFLICWLPYASVAFYIFTHQGSNFGPVFMTIPAFFAKSSSIYNPVIYIMMN 900 Query 311 KQFRNCMLTTICCGKNPLGDDEASAT 336 KQFRNCMLTT+CCGKNPLGDDEAS T Sbjct 901 KQFRNCMLTTLCCGKNPLGDDEASTT 978 ><a name=Subject_2></a> gi|2734705|gb|U59921.1|BBU59921 Bufo bufo rhodopsin mRNA, complete cds Length=1574 Score = 646 bits (1489), Expect = 0.0, Method: Compositional matrix adjust. Identities = 290/342 (85%), Positives = 320/342 (94%), Gaps = 1/342 (0%) Frame = +3 Query 1 MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY 60 MNGTEGPNFY+P SN TGVVRSPFEYPQYYLAEPWQ+S+L AYMFLLI+LGFPINF+TLY Sbjct 42 MNGTEGPNFYIPMSNKTGVVRSPFEYPQYYLAEPWQYSILCAYMFLLILLGFPINFMTLY 221 Query 61 VTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG 120 VT+QHKKLRTPLNYILLNLA A+ FMVL GFT T+Y+S+ GYF+ G TGC +EGFFATLG Sbjct 222 VTIQHKKLRTPLNYILLNLAFANHFMVLCGFTVTMYSSMNGYFILGATGCYVEGFFATLG 401 Query 121 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIP 180 GEIALWSLVVLAIERYVVVCKPMSNFRF ENHA+MGVAFTW+MAL+CA PPL GWSRYIP Sbjct 402 GEIALWSLVVLAIERYVVVCKPMSNFRFSENHAVMGVAFTWIMALSCAVPPLLGWSRYIP 581 Query 181 EGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQES 240 EG+QCSCG+DYYTLKPEVNNESFVIYMFVVHFTIP+IIIFFCYG+LV TVKEAAAQQQES Sbjct 582 EGMQCSCGVDYYTLKPEVNNESFVIYMFVVHFTIPLIIIFFCYGRLVCTVKEAAAQQQES 761 Query 241 ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAI 300 ATTQKAEKEVTRMVIIMV+ FLICWVPYASVAF+IF+ QGS FGPIFMT+PAFFAKS++I Sbjct 762 ATTQKAEKEVTRMVIIMVVFFLICWVPYASVAFFIFSNQGSEFGPIFMTVPAFFAKSSSI 941 Query 301 YNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEA-SATVSKTE 341 YNPVIYIM+NKQFRNCM+TT+CCGKNP G+D+A SA SKTE Sbjct 942 YNPVIYIMLNKQFRNCMITTLCCGKNPFGEDDASSAATSKTE 1067 ><a name=Subject_6></a> gi|12583664|dbj|AB043817.1| Conger myriaster conf gene for fresh water form rod opsin, complete cds Length=1344 Score = 626 bits (1444), Expect = 0.0, Method: Compositional matrix adjust. Identities = 281/342 (82%), Positives = 311/342 (91%), Gaps = 1/342 (0%) Frame = +2 Query 1 MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY 60 MNGTEGPNFY+P SNATGVVRSPFEYPQYYLAEPW FS L+AYMF LI+ GFPINFLTLY Sbjct 23 MNGTEGPNFYIPMSNATGVVRSPFEYPQYYLAEPWAFSALSAYMFFLIIAGFPINFLTLY 202 Query 61 VTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLG 120 VT++HKKLRTPLNYILLNLAVADLFMV GGFT+T+YTS+HGYFVFGPTGCN+EGFFATLG Sbjct 203 VTIEHKKLRTPLNYILLNLAVADLFMVFGGFTTTMYTSMHGYFVFGPTGCNIEGFFATLG 382 Query 121 GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIP 180 GEIALW LVVLAIER++VVCKP++NFRFGE HAIMGV TW MALACA PPL GWSRYIP Sbjct 383 GEIALWCLVVLAIERWMVVCKPVTNFRFGESHAIMGVMVTWTMALACALPPLFGWSRYIP 562 Query 181 EGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQES 240 EGLQCSCGIDYYT P +NNESFVIYMF HF+IP+ +I FCYG+LV TVKEAAAQQQES Sbjct 563 EGLQCSCGIDYYTRAPGINNESFVIYMFTCHFSIPLAVISFCYGRLVCTVKEAAAQQQES 742 Query 241 ATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAI 300 TTQ+AE+EVTRMV+IMVI+FL+CWVPYASVA YIFTHQGS FGPIFMTIP+FFAKS+A+ Sbjct 743 ETTQRAEREVTRMVVIMVISFLVCWVPYASVAWYIFTHQGSTFGPIFMTIPSFFAKSSAL 922 Query 301 YNPVIYIMMNKQFRNCMLTTICCGKNPL-GDDEASATVSKTE 341 YNP+IYI MNKQFR CM+TT+CCGKNP +D ASAT SKTE Sbjct 923 YNPMIYICMNKQFRHCMITTLCCGKNPFEEEDGASATSSKTE 1048 ><a name=Subject_3></a> gi|283855845|gb|GQ290303.1| Cynopterus brachyotis voucher 20020434 rhodopsin (RHO) gene, exons 1 through 5 and partial cds Length=4301 Score = 151 bits (342), Expect(2) = 1e-71, Method: Compositional matrix adjust. Identities = 69/74 (93%), Positives = 73/74 (99%), Gaps = 0/74 (0%) Frame = +3 Query 239 ESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSA 298 ESATTQKAEKEVTRMVIIMVIAFLICW+PYA VAFYIFTHQGSNFGPIFMT+PAFFAKS+ Sbjct 3147 ESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSNFGPIFMTLPAFFAKSS 3326 Query 299 AIYNPVIYIMMNKQ 312 +IYNPVIYIMMNKQ Sbjct 3327 SIYNPVIYIMMNKQ 3368 Score = 126 bits (284), Expect(2) = 1e-71, Method: Compositional matrix adjust. Identities = 54/59 (92%), Positives = 57/59 (97%), Gaps = 0/59 (0%) Frame = +2 Query 177 RYIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAA 235 RYIPEG+QCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMI+IFFCYGQLVFTVKE + Sbjct 2855 RYIPEGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIVIFFCYGQLVFTVKEVRS 3031 Score = 229 bits (523), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 107/111 (96%), Positives = 109/111 (98%), Gaps = 0/111 (0%) Frame = +1 Query 11 VPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT 70 VPFSN TGVVRSPFE+PQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT Sbjct 1 VPFSNKTGVVRSPFEHPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRT 180 Query 71 PLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLGG 121 PLNYILLNLAVADLFMV GGFT+TLYTSLHGYFVFGPTGCNLEGFFATLGG Sbjct 181 PLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGG 333 Score = 122 bits (276), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 55/59 (93%), Positives = 56/59 (95%), Gaps = 0/59 (0%) Frame = +3 Query 119 LGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSR 177 L GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMG+A TWVMALACAAPPL GWSR Sbjct 1404 LAGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGLALTWVMALACAAPPLVGWSR 1580 Score = 57.7 bits (125), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 23/26 (88%), Positives = 24/26 (92%), Gaps = 0/26 (0%) Frame = +1 Query 312 QFRNCMLTTICCGKNPLGDDEASATV 337 QFRNCMLTT+CCGKNPLGDDEAS T Sbjct 4222 QFRNCMLTTLCCGKNPLGDDEASTTA 4299 Lambda K H a alpha 0.351 0.182 0.707 0.522 1.92 Gapped Lambda K H a alpha sigma 0.299 0.0710 0.270 1.10 13.8 14.5 Effective search space used: 1049670 Database: User specified sequence set (Input: rhodopsin_nucs.fasta). Posted date: Unknown Number of letters in database: 10,296 Number of sequences in database: 6 Matrix: BLOSUM80 Gap Penalties: Existence: 10, Extension: 1 Neighboring words threshold: 14 Window for multiple hits: 25 </PRE> </BODY> </HTML>