Mercurial > repos > devteam > picard
annotate picard_MeanQualityByCycle.xml @ 8:3a3234d7a2e8 draft
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
| author | devteam |
|---|---|
| date | Thu, 16 Jul 2015 15:53:10 -0400 |
| parents | 3d4f1fa26f0e |
| children | 05087b27692a |
| rev | line source |
|---|---|
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3a3234d7a2e8
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
devteam
parents:
5
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changeset
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1 <tool name="MeanQualityByCycle" id="picard_MeanQualityByCycle" version="@TOOL_VERSION@.0"> |
| 5 | 2 <description>chart distribution of base qualities</description> |
| 3 <macros> | |
| 4 <import>picard_macros.xml</import> | |
| 5 </macros> | |
|
8
3a3234d7a2e8
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
devteam
parents:
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diff
changeset
|
6 <expand macro="requirements"> |
|
3a3234d7a2e8
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
devteam
parents:
5
diff
changeset
|
7 <requirement type="package" version="3.1.2">R</requirement> |
|
3a3234d7a2e8
planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/picard commit 00a7926c285bc4a339bd7deebf40b28f39c7d947-dirty
devteam
parents:
5
diff
changeset
|
8 </expand> |
| 5 | 9 <command> |
| 10 @java_options@ | |
| 11 ##set up input files | |
| 12 | |
| 13 #set $reference_fasta_filename = "localref.fa" | |
| 14 | |
| 15 #if str( $reference_source.reference_source_selector ) == "history": | |
| 16 ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" && | |
| 17 #else: | |
| 18 #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path ) | |
| 19 #end if | |
| 20 | |
| 21 java -jar \$JAVA_JAR_PATH/picard.jar | |
| 22 MeanQualityByCycle | |
| 23 INPUT="${inputFile}" | |
| 24 OUTPUT="${outFile}" | |
| 25 CHART_OUTPUT="${pdfFile}" | |
| 26 REFERENCE_SEQUENCE="${reference_fasta_filename}" | |
| 27 ALIGNED_READS_ONLY="${aligned_reads_only}" | |
| 28 PF_READS_ONLY="${pf_reads_only}" | |
| 29 | |
| 30 ASSUME_SORTED="${assume_sorted}" | |
| 31 | |
| 32 VALIDATION_STRINGENCY="${validation_stringency}" | |
| 33 QUIET=true | |
| 34 VERBOSITY=ERROR | |
| 35 | |
| 36 </command> | |
| 37 <inputs> | |
| 38 <param format="sam,bam" name="inputFile" type="data" label="SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset."/> | |
| 39 <conditional name="reference_source"> | |
| 40 <param name="reference_source_selector" type="select" label="Load reference genome from"> | |
| 41 <option value="cached">Local cache</option> | |
| 42 <option value="history">History</option> | |
| 43 </param> | |
| 44 <when value="cached"> | |
| 45 <param name="ref_file" type="select" label="Using reference genome" help="REFERENCE_SEQUENCE"> | |
| 46 <options from_data_table="all_fasta"> | |
| 47 </options> | |
| 48 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> | |
| 49 </param> | |
| 50 </when> | |
| 51 <when value="history"> | |
| 52 <param name="ref_file" type="data" format="fasta" label="Use the folloing dataset as the reference sequence" help="REFERENCE_SEQUENCE; You can upload a FASTA sequence to the history and use it as reference" /> | |
| 53 </when> | |
| 54 </conditional> | |
| 55 <param name="aligned_reads_only" type="boolean" truevalue="true" falsevalue="false" label="If set to true, calculate mean quality over aligned reads only" help="ALIGNED_READS_ONLY; default=False"/> | |
| 56 <param name="pf_reads_only" type="boolean" truevalue="true" falsevalue="false" label="If set to true calculate mean quality over reads passing quality filter" help="PF_READS_ONLY; default=False"/> | |
| 57 <param name="assume_sorted" type="boolean" label="Assume the input file is already sorted" checked="true" truevalue="true" falsevalue="false" help="ASSUME_SORTED"/> | |
| 58 | |
| 59 <expand macro="VS" /> | |
| 60 | |
| 61 </inputs> | |
| 62 | |
| 63 <outputs> | |
| 64 <data format="tabular" name="outFile" label="${tool.name} on ${on_string}: Summary data"/> | |
| 65 <data format="pdf" name="pdfFile" label="${tool.name} on ${on_string}: Chart PDF"/> | |
| 66 </outputs> | |
| 67 | |
| 68 <tests> | |
| 69 <test> | |
| 70 <param name="assume_sorted" value="true" /> | |
| 71 <param name="aligned_reads_only" value="false" /> | |
| 72 <param name="pf_reads_only" value="false" /> | |
| 73 <param name="reference_source_selector" value="history" /> | |
| 74 <param name="ref_file" value="picard_MeanQualityByCycle_ref.fa" /> | |
| 75 <param name="inputFile" value="picard_MeanQualityByCycle.bam" ftype="bam" /> | |
| 76 <output name="outFile" file="picard_MeanQualityByCycle_test1.tab" ftype="tabular" lines_diff="4"/> | |
| 77 </test> | |
| 78 </tests> | |
| 79 | |
| 80 <stdio> | |
| 81 <exit_code range="1:" level="fatal"/> | |
| 82 </stdio> | |
| 83 | |
| 84 <help> | |
| 85 | |
| 86 .. class:: infomark | |
| 87 | |
| 88 **Purpose** | |
| 89 | |
| 90 Program to chart the distribution of base qualities by cycle within reads supplied in a SAM or BAM dataset. | |
| 91 | |
| 92 @dataset_collections@ | |
| 93 | |
| 94 @description@ | |
| 95 | |
| 96 ALIGNED_READS_ONLY=Boolean If set to true, calculate the base distribution over aligned reads only. Default value: | |
| 97 false. Possible values: {true, false} | |
| 98 | |
| 99 PF_READS_ONLY=Boolean If set to true calculate the base distribution over PF reads only. Default value: false. | |
| 100 This option can be set to 'null' to clear the default value. Possible values: {true, | |
| 101 false} | |
| 102 | |
| 103 ASSUME_SORTED=Boolean | |
| 104 AS=Boolean If true (default), then the sort order in the header file will be ignored. Default: True | |
| 105 | |
| 106 @more_info@ | |
| 107 | |
| 108 </help> | |
| 109 </tool> | |
| 110 | |
| 111 |