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1 <tool name="CollectBaseDistributionByCycle" id="picard_CollectBaseDistributionByCycle" version="1.126.0">
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2 <description>charts the nucleotide distribution per cycle in a SAM or BAM dataset</description>
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3 <requirements>
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4 <requirement type="package" version="1.126.0">picard</requirement>
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5 </requirements>
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6
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7 <macros>
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8 <import>picard_macros.xml</import>
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9 </macros>
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10
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11 <command>
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12 @java_options@
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13 ##set up input files
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14
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15 #set $reference_fasta_filename = "localref.fa"
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16
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17 #if str( $reference_source.reference_source_selector ) == "history":
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18 ln -s "${reference_source.ref_file}" "${reference_fasta_filename}" &&
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19 #else:
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20 #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path )
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21 #end if
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22
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23 java -jar \$JAVA_JAR_PATH/picard.jar
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24 CollectBaseDistributionByCycle
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25 INPUT="${inputFile}"
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26 OUTPUT="${outFile}"
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27 CHART_OUTPUT="${pdfFile}"
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28 ALIGNED_READS_ONLY="${aligned_reads_only}"
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29 PF_READS_ONLY="${pf_reads_only}"
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30 REFERENCE_SEQUENCE="${reference_fasta_filename}"
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31 ASSUME_SORTED="${assume_sorted}"
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32
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33 VALIDATION_STRINGENCY="${validation_stringency}"
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34 QUIET=true
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35 VERBOSITY=ERROR
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36
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37 </command>
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38 <inputs>
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39 <param format="sam,bam" name="inputFile" type="data" label="Select SAM/BAM dataset or dataset collection" help="If empty, upload or import a SAM/BAM dataset."/>
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40 <conditional name="reference_source">
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41 <param name="reference_source_selector" type="select" label="Load reference genome from">
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42 <option value="cached">Local cache</option>
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43 <option value="history">History</option>
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44 </param>
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45 <when value="cached">
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46 <param name="ref_file" type="select" label="Using reference genome" help="REFERENCE_SEQUENCE">
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47 <options from_data_table="all_fasta">
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48 </options>
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49 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
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50 </param>
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51 </when>
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52 <when value="history">
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53 <param name="ref_file" type="data" format="fasta" label="Use the folloing dataset as the reference sequence" help="REFERENCE_SEQUENCE; You can upload a FASTA sequence to the history and use it as reference" />
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54 </when>
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55 </conditional>
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56 <param name="aligned_reads_only" type="boolean" label="Calculate the base distribution over aligned reads only" checked="true" truevalue="true" falsevalue="false" help="ALIGNED_READS_ONLY"/>
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57 <param name="pf_reads_only" type="boolean" label="Calculate the base distribution over PF (passing filtering) reads only" checked="true" truevalue="true" falsevalue="false" help="PF_READS_ONLY"/>
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58 <param name="assume_sorted" type="boolean" label="Assume the input file is already sorted" checked="true" truevalue="true" falsevalue="false" help="ASSUME_SORTED"/>
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59
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60 <expand macro="VS" />
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61
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62 </inputs>
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63
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64 <outputs>
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65 <data format="tabular" name="outFile"/>
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66 <data format="pdf" name="pdfFile"/>
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67 </outputs>
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68
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69 <tests>
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70 <test>
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71 <param name="aligned_reads_only" value="true" />
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72 <param name="pf_reads_only" value="true" />
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73 <param name="assume_sorted" value="true" />
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74 <param name="reference_source_selector" value="history" />
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75 <param name="ref_file" value="picard_CollectBaseDistributionByCycle_ref.fa" />
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76 <param name="inputFile" value="picard_CollectBaseDistributionByCycle.bam" ftype="bam" />
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77 <output name="outFile" file="picard_CollectBaseDistributionByCycle_test1.tab" ftype="tabular" lines_diff="4"/>
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78 </test>
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79 </tests>
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80
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81 <stdio>
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82 <exit_code range="1:" level="fatal"/>
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83 </stdio>
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84
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85 <help>
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86
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87 .. class:: infomark
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88
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89 **Purpose**
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90
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91 Program to chart the nucleotide distribution per cycle in a SAM or BAM file.
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92
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93 @dataset_collections@
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94
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95 @description@
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96
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97 ALIGNED_READS_ONLY=Boolean If set to true, calculate the base distribution over aligned reads only. Default value:
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98 false. This option can be set to 'null' to clear the default value. Possible values:
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99 {true, false}
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100
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101 PF_READS_ONLY=Boolean If set to true calculate the base distribution over PF reads only. Default value: false.
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102 This option can be set to 'null' to clear the default value. Possible values: {true,
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103 false}
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104
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105 REFERENCE_SEQUENCE=File
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106 R=File Reference sequence fasta Default value: null.
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107
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108 ASSUME_SORTED=Boolean
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109 AS=Boolean If true (default), then the sort order in the header file will be ignored. Default
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110
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111 @more_info@
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112
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113 </help>
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114 </tool>
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115
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116
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