annotate quality_filter.py @ 3:2f4393f9f912 draft

planemo upload commit 00a7926c285bc4a339bd7deebf40b28f39c7d947
author devteam
date Tue, 21 Jul 2015 15:40:35 -0400
parents 6c6f15373f96
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
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1 #!/usr/bin/env python
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2 #Guruprasad Ananda
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3 """
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4 Filter based on nucleotide quality (PHRED score).
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5
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6 usage: %prog input out_file primary_species mask_species score mask_char mask_region mask_region_length
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7 """
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8
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9
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10 from __future__ import division
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11 from galaxy import eggs
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12 import pkg_resources
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13 pkg_resources.require( "lrucache" )
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14 import numpy
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15
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16 import sys
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17 import os, os.path
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18 from UserDict import DictMixin
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19 from bx.binned_array import FileBinnedArray
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20 from bx.bitset import *
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21 from bx.bitset_builders import *
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22 from bx.cookbook import doc_optparse
3
2f4393f9f912 planemo upload commit 00a7926c285bc4a339bd7deebf40b28f39c7d947
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23 from galaxy.tools.exception_handling import *
0
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24 import bx.align.maf
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25
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26 class FileBinnedArrayDir( DictMixin ):
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27 """
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28 Adapter that makes a directory of FileBinnedArray files look like
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29 a regular dict of BinnedArray objects.
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30 """
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31 def __init__( self, dir ):
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32 self.dir = dir
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33 self.cache = dict()
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34 def __getitem__( self, key ):
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35 value = None
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36 if key in self.cache:
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37 value = self.cache[key]
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38 else:
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39 fname = os.path.join( self.dir, "%s.qa.bqv" % key )
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40 if os.path.exists( fname ):
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41 value = FileBinnedArray( open( fname ) )
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42 self.cache[key] = value
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43 if value is None:
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44 raise KeyError( "File does not exist: " + fname )
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45 return value
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46
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47 def stop_err(msg):
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48 sys.stderr.write(msg)
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49 sys.exit()
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50
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51 def load_scores_ba_dir( dir ):
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52 """
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53 Return a dict-like object (keyed by chromosome) that returns
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54 FileBinnedArray objects created from "key.ba" files in `dir`
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55 """
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56 return FileBinnedArrayDir( dir )
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57
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58 def bitwise_and ( string1, string2, maskch ):
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59 result = []
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60 for i, ch in enumerate(string1):
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61 try:
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62 ch = int(ch)
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63 except:
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64 pass
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65 if string2[i] == '-':
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66 ch = 1
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67 if ch and string2[i]:
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68 result.append(string2[i])
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69 else:
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70 result.append(maskch)
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71 return ''.join(result)
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72
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73 def main():
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74 # Parsing Command Line here
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75 options, args = doc_optparse.parse( __doc__ )
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76
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77 try:
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78 #chr_col_1, start_col_1, end_col_1, strand_col_1 = parse_cols_arg( options.cols )
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79 inp_file, out_file, pri_species, mask_species, qual_cutoff, mask_chr, mask_region, mask_length, loc_file = args
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80 qual_cutoff = int(qual_cutoff)
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81 mask_chr = int(mask_chr)
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82 mask_region = int(mask_region)
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83 if mask_region != 3:
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84 mask_length = int(mask_length)
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85 else:
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86 mask_length_r = int(mask_length.split(',')[0])
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87 mask_length_l = int(mask_length.split(',')[1])
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88 except:
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89 stop_err( "Data issue, click the pencil icon in the history item to correct the metadata attributes of the input dataset." )
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90
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91 if pri_species == 'None':
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92 stop_err( "No primary species selected, try again by selecting at least one primary species." )
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93 if mask_species == 'None':
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94 stop_err( "No mask species selected, try again by selecting at least one species to mask." )
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95
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96 mask_chr_count = 0
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97 mask_chr_dict = {0:'#', 1:'$', 2:'^', 3:'*', 4:'?', 5:'N'}
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98 mask_reg_dict = {0:'Current pos', 1:'Current+Downstream', 2:'Current+Upstream', 3:'Current+Both sides'}
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99
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100 #ensure dbkey is present in the twobit loc file
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101 try:
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102 pspecies_all = pri_species.split(',')
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103 pspecies_all2 = pri_species.split(',')
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104 pspecies = []
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105 filepaths = []
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106 for line in open(loc_file):
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107 if pspecies_all2 == []:
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108 break
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109 if line[0:1] == "#":
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110 continue
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111 fields = line.split('\t')
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112 try:
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113 build = fields[0]
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114 for i, dbkey in enumerate(pspecies_all2):
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115 if dbkey == build:
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116 pspecies.append(build)
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117 filepaths.append(fields[1])
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118 del pspecies_all2[i]
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119 else:
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120 continue
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121 except:
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122 pass
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123 except Exception, exc:
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124 stop_err( 'Initialization errorL %s' % str( exc ) )
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125
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126 if len(pspecies) == 0:
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127 stop_err( "Quality scores are not available for the following genome builds: %s" % ( pspecies_all2 ) )
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128 if len(pspecies) < len(pspecies_all):
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129 print "Quality scores are not available for the following genome builds: %s" % (pspecies_all2)
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130
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131 scores_by_chrom = []
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132 #Get scores for all the primary species
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133 for file in filepaths:
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134 scores_by_chrom.append(load_scores_ba_dir( file.strip() ))
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135
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136 try:
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137 maf_reader = bx.align.maf.Reader( open(inp_file, 'r') )
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138 maf_writer = bx.align.maf.Writer( open(out_file,'w') )
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139 except Exception, e:
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140 stop_err( "Your MAF file appears to be malformed: %s" % str( e ) )
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141
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142 maf_count = 0
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143 for block in maf_reader:
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144 status_strings = []
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145 for seq in range (len(block.components)):
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146 src = block.components[seq].src
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147 dbkey = src.split('.')[0]
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148 chr = src.split('.')[1]
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149 if not (dbkey in pspecies):
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150 continue
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151 else: #enter if the species is a primary species
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152 index = pspecies.index(dbkey)
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153 sequence = block.components[seq].text
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154 s_start = block.components[seq].start
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155 size = len(sequence) #this includes the gaps too
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156 status_str = '1'*size
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157 status_list = list(status_str)
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158 if status_strings == []:
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159 status_strings.append(status_str)
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160 ind = 0
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161 s_end = block.components[seq].end
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162 #Get scores for the entire sequence
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163 try:
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164 scores = scores_by_chrom[index][chr][s_start:s_end]
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165 except:
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166 continue
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167 pos = 0
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168 while pos < (s_end-s_start):
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169 if sequence[ind] == '-': #No score for GAPS
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170 ind += 1
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171 continue
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172 score = scores[pos]
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173 if score < qual_cutoff:
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174 score = 0
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175
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176 if not(score):
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177 if mask_region == 0: #Mask Corresponding position only
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178 status_list[ind] = '0'
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179 ind += 1
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180 pos += 1
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181 elif mask_region == 1: #Mask Corresponding position + downstream neighbors
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182 for n in range(mask_length+1):
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183 try:
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184 status_list[ind+n] = '0'
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185 except:
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186 pass
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187 ind = ind + mask_length + 1
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188 pos = pos + mask_length + 1
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189 elif mask_region == 2: #Mask Corresponding position + upstream neighbors
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190 for n in range(mask_length+1):
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191 try:
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192 status_list[ind-n] = '0'
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193 except:
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194 pass
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195 ind += 1
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196 pos += 1
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197 elif mask_region == 3: #Mask Corresponding position + neighbors on both sides
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198 for n in range(-mask_length_l, mask_length_r+1):
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199 try:
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200 status_list[ind+n] = '0'
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201 except:
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202 pass
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203 ind = ind + mask_length_r + 1
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204 pos = pos + mask_length_r + 1
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205 else:
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206 pos += 1
8d65bbc52dfe Imported from capsule None
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207 ind += 1
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208
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209 status_strings.append(''.join(status_list))
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210
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211 if status_strings == []: #this block has no primary species
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212 continue
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213 output_status_str = status_strings[0]
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214 for stat in status_strings[1:]:
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215 try:
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216 output_status_str = bitwise_and (status_strings[0], stat, '0')
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217 except Exception, e:
8d65bbc52dfe Imported from capsule None
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218 break
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219
8d65bbc52dfe Imported from capsule None
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220 for seq in range (len(block.components)):
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221 src = block.components[seq].src
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222 dbkey = src.split('.')[0]
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223 if dbkey not in mask_species.split(','):
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224 continue
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225 sequence = block.components[seq].text
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226 sequence = bitwise_and (output_status_str, sequence, mask_chr_dict[mask_chr])
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227 block.components[seq].text = sequence
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228 mask_chr_count += output_status_str.count('0')
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229 maf_writer.write(block)
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230 maf_count += 1
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231
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232 maf_reader.close()
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233 maf_writer.close()
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234 print "No. of blocks = %d; No. of masked nucleotides = %s; Mask character = %s; Mask region = %s; Cutoff used = %d" % (maf_count, mask_chr_count, mask_chr_dict[mask_chr], mask_reg_dict[mask_region], qual_cutoff)
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235
8d65bbc52dfe Imported from capsule None
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236
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237 if __name__ == "__main__":
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238 main()