Mercurial > repos > ebi-gxa > salmon_kallisto_mtx_to_10x
annotate salmonKallistoMtxTo10x.xml @ 6:17f7e1e51fad draft
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit c06e0424fd25356d1a8c172fb92b383fd9d28e90
author | ebi-gxa |
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date | Thu, 21 Nov 2019 09:38:48 -0500 |
parents | cd6007b47199 |
children | 60fa6080f86f |
rev | line source |
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17f7e1e51fad
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit c06e0424fd25356d1a8c172fb92b383fd9d28e90
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1 <tool id="_salmon_kallisto_mtx_to_10x" name="salmonKallistoMtxTo10x" version="0.0.1+galaxy2"> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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2 <description>Transforms .mtx matrix and associated labels into a format compatible with tools expecting old-style 10X data</description> |
fe0fd27aba50
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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3 <requirements> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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4 <requirement type="package">scipy</requirement> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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5 <requirement type="package">pandas</requirement> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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6 </requirements> |
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40f7a3d18cf4
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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7 <command detect_errors="exit_code"><![CDATA[ |
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336ae8f0a0b2
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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8 file $mtx_file | grep 'gzip compressed' > /dev/null; |
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40f7a3d18cf4
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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9 if [ $? -eq 0 ]; then |
40f7a3d18cf4
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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10 matrixfile=matrix.mtx.gz; |
40f7a3d18cf4
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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11 else |
40f7a3d18cf4
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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12 matrixfile=matrix.mtx; |
40f7a3d18cf4
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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13 fi; |
40f7a3d18cf4
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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14 ln -s ${mtx_file} \$matrixfile; |
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cd6007b47199
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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15 $__tool_directory__/salmonKallistoMtxTo10x.py --cell_prefix "${cell_prefix}" "\${matrixfile}" "${genes_file}" "${barcodes_file}" ./ |
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fe0fd27aba50
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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16 ]]></command> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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17 |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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18 <inputs> |
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a2aaefceb418
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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19 <param name="mtx_file" type="data" format="mtx" label=".mtx-format matrix" /> |
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fe0fd27aba50
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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20 <param name="genes_file" type="data" format="txt" label="Tab-delimited genes file" /> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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21 <param name="barcodes_file" type="data" format="txt" label="Tab-delimited barcodes file" /> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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22 <param name="cell_prefix" type="text" optional='true' value="" label="Prefix to prepend to cell names / barcodes" help="This is useful when multiple matrices from different libraries with overlapping barcodes will be merged"/> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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23 </inputs> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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24 |
fe0fd27aba50
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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25 <outputs> |
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17f7e1e51fad
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit c06e0424fd25356d1a8c172fb92b383fd9d28e90
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26 <data name="genes_out" format="tabular" from_work_dir="genes.tsv" label="${tool.name} on ${on_string}: genes"/> |
17f7e1e51fad
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit c06e0424fd25356d1a8c172fb92b383fd9d28e90
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27 <data name="barcodes_out" format="tabular" from_work_dir="barcodes.tsv" label="${tool.name} on ${on_string}: barcodes"/> |
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a2aaefceb418
planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 3fbbd818012005da4513271beda50df98d3c1cde-dirty
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28 <data name="matrix_out" format="mtx" from_work_dir="matrix.mtx" label="${tool.name} on ${on_string}: matrix" /> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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29 </outputs> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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30 |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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31 <help><![CDATA[ |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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32 .. class:: infomark |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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33 |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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34 **What it does** |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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35 |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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36 Kallisto and Alevin (and possibly other tools) output an MTX file and associated labels that are not consistent with the old-style 10X, meaning that routines designed to parse those files cannot be used. This tool transforms (in the mathematical sense) the matrix, and reformats the genes file (essentially duplicating the column) to match those earlier conventions. |
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37 |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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38 **Inputs** |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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39 |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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40 MTX and row and gene labels from the relevant tool. For Alevin this will be |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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41 |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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42 * Matrix file: quants_mat.mtx.gz |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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43 * Genes file: quants_mat_cols.txt |
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44 * Barcodes file: quants_mat_rows.txt |
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45 |
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46 For Kallisto it will be: |
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47 |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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48 * Matrix file: [name].mtx.gz |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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49 * Genes file: [name].genes.txt |
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50 * Barcodes file: [name].barcodes.txt |
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51 |
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52 ----- |
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53 |
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54 **Outputs** |
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55 |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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56 * MTX, gene and cell labels in 10X style |
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57 ]]></help> |
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58 <citations> |
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59 <citation type="bibtex"> |
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60 @misc{github-hinxton-single-cell, |
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61 author = {Jonathan Manning, EBI Gene Expression Team}, |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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62 year = {2019}, |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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63 title = {Hinxton Single Cell Anlysis Environment}, |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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64 publisher = {GitHub}, |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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65 journal = {GitHub repository}, |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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66 url = {https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary}, |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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67 }</citation> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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68 </citations> |
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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/salmon-kallisto-mtx-to-10x/.shed.yml commit 023431ca119829efbde33c94d54e051fac24a1d5
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69 </tool> |