Mercurial > repos > ebi-gxa > scanpy_filter_cells

annotate scanpy-filter-cells.xml @ 4:1b0fdf08a454 draft

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planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 1d069e25584e436d7628694cb8853a15f1f85604
author ebi-gxa
date Wed, 20 Nov 2019 05:18:49 -0500
parents 8e48fa9618c1
children 9b2072ee4bdd
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9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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1 <?xml version="1.0" encoding="utf-8"?>
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1b0fdf08a454 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 1d069e25584e436d7628694cb8853a15f1f85604
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2 <tool id="scanpy_filter_cells" name="Scanpy FilterCells" version="@TOOL_VERSION@+galaxy0">
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3 <description>based on counts and numbers of genes expressed</description>
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4 <macros>
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5 <import>scanpy_macros2.xml</import>
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6 </macros>
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7 <expand macro="requirements"/>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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8 <command detect_errors="exit_code"><![CDATA[
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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9 ln -s '${input_obj_file}' input.h5 &&
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10 PYTHONIOENCODING=utf-8 scanpy-filter-cells
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11 #if $gene_name
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12 --gene-name '${gene_name}'
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13 #end if
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14 #if $parameters
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15 #set pars = ' '.join(['--param c:{name} {min} {max}'.format(**$p) for $p in $parameters])
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16 ${pars}
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17 #end if
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18 #if $categories
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19 #set cats = ' '.join(["--category c:{name} '{negate}{values}'".format(**$c) for $c in $categories])
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20 ${cats}
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21 #end if
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22 #if $subsets
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23 #set subs = ' '.join(['--subset c:{name} {subset}'.format(**$s) for $s in $subsets])
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24 ${subs}
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25 #end if
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26 @INPUT_OPTS@
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27 @OUTPUT_OPTS@
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28 @EXPORT_MTX_OPTS@
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29 ]]></command>
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31 <inputs>
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32 <expand macro="input_object_params"/>
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33 <expand macro="output_object_params"/>
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34
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35 <param name="gene_name" type="text" optional="true" label="Name of the column in `anndata.var` that contains gene name"
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36 help="Used for flagging mitochondria genes (starting with 'MT-'). Leave empty if gene table already has a boolean column called 'mito' that flags MT genes"/>
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37
0
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38 <repeat name="parameters" title="Parameters used to filter cells" min="1">
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39 <param name="name" type="text" value="n_genes" label="Name of parameter to filter on" help="for example n_genes or n_counts">
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40 <option value="n_genes">n_genes</option>
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41 <option value="n_counts">n_counts</option>
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42 <option value="pct_counts_mito">pct_counts_mito (only usable if MT genes are flagged)</option>
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43 </param>
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44 <param name="min" type="float" value="0" min="0" label="Min value"/>
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45 <param name="max" type="float" value="1e9" label="Max value"/>
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46 </repeat>
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48 <repeat name="categories" title="Categories used to filter cells" min="0">
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49 <param name="name" type="text" value="" label="Name of the categorical variable to filter on"/>
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50 <param name="values" type="text" value="" label="Comma-separated list of categories"/>
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51 <param name="negate" type="boolean" truevalue="!" falsevalue="" checked="false" label="Apply as negative filter" help="If enabled, specified categories will be removed rather than retained."/>
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52 </repeat>
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54 <repeat name="subsets" title="Subsets used to filter cells" min="0">
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55 <param name="name" type="text" value="" label="Name of the categorical variable to filter on"/>
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56 <param name="subset" type="data" format="tabular" label="List of values to keep" help="A one-column headerless text file is required"/>
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57 </repeat>
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58 <expand macro="export_mtx_params"/>
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59 </inputs>
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60
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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61 <outputs>
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62 <data name="output_h5" format="h5" from_work_dir="output.h5" label="${tool.name} on ${on_string}: Filtered cells"/>
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63 <expand macro="export_mtx_outputs"/>
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64 </outputs>
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65
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66 <tests>
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67 <test>
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68 <param name="input_obj_file" value="read_10x.h5"/>
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69 <param name="input_format" value="anndata"/>
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70 <param name="output_format" value="anndata"/>
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71 <repeat name="parameters">
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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72 <param name="name" value="n_genes"/>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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73 <param name="min" value="200"/>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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74 <param name="max" value="2500"/>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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75 </repeat>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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76 <repeat name="parameters">
2
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77 <param name="name" value="n_counts"/>
0
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78 <param name="min" value="0"/>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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79 <param name="max" value="1e9"/>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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80 </repeat>
1
dcfb23758646 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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81 <output name="output_h5" file="output.h5" ftype="h5" compare="sim_size"/>
0
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82 </test>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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83 </tests>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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84
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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85 <help><![CDATA[
1
dcfb23758646 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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86 ===================================================================
dcfb23758646 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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87 Filter cells based on various QC metrics (`scanpy.pp.filter_cells`)
dcfb23758646 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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88 ===================================================================
0
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89
1
dcfb23758646 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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90 For instance, only keep cells with at least `min_counts` and at most
dcfb23758646 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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91 `max_counts` UMI and/or at least `min_genes` expressed genes and/or at most
dcfb23758646 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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92 `max_mito_percent` mitocondria expression. This is to filter measurement
dcfb23758646 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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93 outliers, i.e., "unreliable" observations.
0
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94
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95 @HELP@
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96
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97 @VERSION_HISTORY@
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98 ]]></help>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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99 <expand macro="citations"/>
9f0ca1641ab2 planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 9bf9a6e46a330890be932f60d1d996dd166426c4
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100 </tool>