annotate scanpy-plot-embedding.xml @ 5:dba78f1681b1 draft

planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 367d978e52fac9467a804009c5013f53c06765f0
author ebi-gxa
date Tue, 26 Nov 2019 05:54:15 -0500
parents 109cfa259d8b
children 49fe4e682059
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c63bc0b71865 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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1 <?xml version="1.0" encoding="utf-8"?>
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2 <tool id="scanpy_plot_embed" name="Scanpy PlotEmbed" version="@TOOL_VERSION@+galaxy6">
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3 <description>visualise cell embeddings</description>
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4 <macros>
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5 <import>scanpy_macros2.xml</import>
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6 </macros>
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7 <expand macro="requirements"/>
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8 <command detect_errors="exit_code"><![CDATA[
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9 ln -s '${input_obj_file}' input.h5 &&
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10 PYTHONIOENCODING=utf-8 scanpy-cli plot embed
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11 @INPUT_OPTS@
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12 --basis '${basis}'
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13 --color '${color_by}'
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14 ${use_raw}
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15 --legend-loc '${legend_loc}'
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16 --legend-fontsize '${legend_fontsize}'
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17 #if $point_size
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18 --size ${point_size}
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19 #end if
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20 #if $gene_symbols_field
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21 --gene-symbols ${gene_symbols_field}
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22 #end if
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23 @PLOT_OPTS@
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24 ]]></command>
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25
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26 <inputs>
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27 <expand macro="input_object_params"/>
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28 <param name="basis" argument="--basis" type="text" label="Name of the embedding to plot"/>
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29 <param name="color_by" argument="--color" type="text" label="Color by attributes, comma separated texts">
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30 <sanitizer>
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31 <valid initial="string.printable"/>
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32 </sanitizer>
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33 </param>
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34 <param name="gene_symbols_field" argument="--gene-symbols" type="text" optional="true" label="Field for gene symbols" help="The field used in the AnnData object to store gene symbols, leave blank for the default (index), else a common value is 'gene_symbols'"/>
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35 <param name="use_raw" argument="--use-raw" type="boolean" checked="true" truevalue="--use-raw" falsevalue="--no-raw" label="Use raw attributes if present"/>
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36 <param name="legend_loc" argument="--legend-loc" type="select" label="Location of legend">
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37 <option value="right margin" selected="true">Right margin</option>
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38 <option value="on data">On data</option>
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39 </param>
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40 <param name="legend_fontsize" argument="--legend-fontsize" type="integer" value="15" label="Legend font size"/>
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41 <param name="point_size" argument="--size" type="integer" optional="true" label="Point size"
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42 help="Automatically determined if not specified."/>
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43 <expand macro="output_plot_params"/>
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44 </inputs>
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45
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46 <outputs>
4
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47 <data name="output_png" format="png" from_work_dir="output.png" label="${tool.name} on ${on_string}: ${basis} embedding plot"/>
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48 </outputs>
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49
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50 <tests>
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51 <test>
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52 <param name="input_obj_file" value="find_cluster.h5"/>
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53 <param name="input_format" value="anndata"/>
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54 <param name="color_by" value="louvain"/>
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55 <output name="output_png" file="run_tsne.png" ftype="png" compare="sim_size"/>
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56 </test>
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57 </tests>
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58
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59 <help><![CDATA[
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60 =============================================================
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61 Plot embeddings of a given method of dimensionality reduction
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62 =============================================================
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63
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64 It yields a scatter plot in png format, wherein cells are placed in space of
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65 reduced dimensionality and coloured by attribute of choice.
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66
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67 Requires calculating the specified embeddings first. For example, to make
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68 UMAP/TSNE plots, run `Scanpy RunUMAP`/`Scanpy RunTSNE` first, then enter
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69 "umap"/"tsne" as the name of the embedding to plot here.
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70
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71 @HELP@
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72
c63bc0b71865 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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73 @VERSION_HISTORY@
c63bc0b71865 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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74 ]]></help>
c63bc0b71865 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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75 <expand macro="citations"/>
c63bc0b71865 "planemo upload for repository https://github.com/ebi-gene-expression-group/container-galaxy-sc-tertiary/tree/develop/tools/tertiary-analysis/scanpy commit 4846776f55931e176f7e77af7c185ec6fec7d142"
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76 </tool>