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1 <tool id="bsmap_meth_caller" name="BSMAP Methylation Caller">
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2 <requirements>
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3 <requirement type='package' version="2.6">bsmap</requirement>
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4 <requirement type='package'>samtools</requirement>
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5 <command interpreter="bash">
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6 bsmap_meth_caller.sh
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7 input=$bsmap_sam
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8 unique=$unique
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9 properly=$properly
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10 zero_meth = $zero_meth
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11 rem_dup = $rem_dup
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12 combine_cpg = $combine_cpg
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13 trimN = $trimN
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14 depth = $depth
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15 output=$output
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16 tempdir=$output.files_path
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17 #if $refGenomeSource.genomeSource == "history":
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18 ref="${refGenomeSource.myFile}"
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19 #else
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20 ref="${refGenomeSource.builtinFile.fields.path}"
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21 #end if
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22
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23 </command>
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24 <inputs>
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25 <conditional name="refGenomeSource">
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26 <param name="genomeSource" type="select" label="Will you select a reference genome from your history or use a built-in reference?" help="Must be the same reference as used for the mapping">
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27 <option value="builtin">Use a built-in reference</option>
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28 <option value="history">Use one from the history</option>
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29 </param>
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30 <when value="builtin">
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31 <param name="builtinFile" type="select" label="Select the reference genome">
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32 <options from_data_table="bsmap_fasta">
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33 <filter type="sort_by" column="2" />
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34 <validator type="no_options" message="No reference genomes are available" />
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35 </options>
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36 </param>
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37 </when>
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38 <when value="history">
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39 <param name="myFile" type="data" format="fasta" label="Select the reference genome" />
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40 </when>
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41 </conditional>
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42
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43 <param name="bsmap_sam" format="sam" type="data" label="BSMAP mapping output file" help="Must be in SAM format" />
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44 <param name="unique" type="boolean" truevalue="true" falsevalue="false" checked="False" label="Process only unique mappings/pairs" />
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45 <param name="properly" type="boolean" truevalue="true" falsevalue="false" checked="False" label="Process only properly paired mappings" />
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46 <param name="zero_meth" type="boolean" truevalue="true" falsevalue="false" checked="True" label="report loci with zero methylation ratios" />
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47 <param name="rem_dup" type="boolean" truevalue="true" falsevalue="false" checked="False" label="Remove duplicated reads" />
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48 <param name="combine_cpg" type="boolean" truevalue="true" falsevalue="false" checked="False" label="Combine CpG methylaion ratios on both strands" />
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49 <param name="trimN" type="integer" value="2" label="Trim N fill-in nucleotides in DNA fragment end-repairing" help="This option is only for pair-end mapping. For RRBS, N could be detetmined by the distance between cuttings sites on forward and reverse strands. For WGBS, N is usually between 0~3" />
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50 <param name="depth" type="integer" value="1" label="Minimum sequencing depth to report loci" />
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51 </inputs>
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52 <outputs>
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53 <data name="output" format ="bed" label="BSMAP methylation output" />
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54 </outputs>
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55 <help>
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56 **What it does**
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57
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58 This methylation caller parses the BSMAP SAM output file into bed format.
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59
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60
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61 **Output format** ::
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62
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63
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64 Column Description
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65 ---------------------- --------------------------------------
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66 1 chr chromosome
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67 2 pos position
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68 3 strand strand
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69 4 context context (CHH,CHG,CpG)
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70 5 coverage totally sequenced Cs at that position
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71 6 methylated methylated Cs at that position
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72 7 percentage methylated percentage of 6
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73 </help>
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74 </tool>
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75
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