Mercurial > repos > eschen42 > w4mclassfilter
annotate w4mclassfilter_wrapper.R @ 13:c18040b6e8b9 draft
"planemo upload for repository https://github.com/HegemanLab/w4mclassfilter_galaxy_wrapper/tree/master commit 1a5dd14c3697516bc55950bd6e46c978af7c19cf"
| author | eschen42 | 
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| date | Thu, 24 Oct 2019 10:21:23 -0400 | 
| parents | 38f509903a0b | 
| children | 87ec0d3c2266 | 
| rev | line source | 
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changeset | 1 #!/usr/bin/env Rscript | 
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changeset | 2 | 
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changeset | 3 library(batch) ## parseCommandArgs | 
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changeset | 4 | 
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changeset | 5 ######## | 
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changeset | 6 # MAIN # | 
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changeset | 7 ######## | 
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changeset | 8 | 
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changeset | 9 argVc <- unlist(parseCommandArgs(evaluate=FALSE)) | 
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changeset | 10 | 
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changeset | 11 ##------------------------------ | 
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changeset | 12 ## Initializing | 
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changeset | 13 ##------------------------------ | 
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changeset | 14 | 
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changeset | 15 ## options | 
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changeset | 16 ##-------- | 
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changeset | 17 | 
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changeset | 18 strAsFacL <- options()$stringsAsFactors | 
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changeset | 19 options(stringsAsFactors = FALSE) | 
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changeset | 20 | 
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changeset | 21 ## libraries | 
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changeset | 22 ##---------- | 
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changeset | 23 | 
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changeset | 24 suppressMessages(library(w4mclassfilter)) | 
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changeset | 25 | 
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"planemo upload for repository https://github.com/HegemanLab/w4mclassfilter_galaxy_wrapper/tree/master commit b9712e554d16ed26f6c6d0c2e8cd74552b49f694"
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changeset | 26 if(packageVersion("w4mclassfilter") < "0.98.12") | 
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"planemo upload for repository https://github.com/HegemanLab/w4mclassfilter_galaxy_wrapper/tree/master commit b9712e554d16ed26f6c6d0c2e8cd74552b49f694"
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changeset | 27 stop("Please use 'w4mclassfilter' versions of 0.98.12 and above") | 
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changeset | 28 | 
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changeset | 29 ## constants | 
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changeset | 30 ##---------- | 
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changeset | 31 | 
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changeset | 32 modNamC <- "w4mclassfilter" ## module name | 
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changeset | 33 | 
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changeset | 34 topEnvC <- environment() | 
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changeset | 35 flgC <- "\n" | 
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changeset | 36 | 
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changeset | 37 ## functions | 
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changeset | 38 ##---------- | 
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changeset | 39 | 
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changeset | 40 flgF <- function(tesC, | 
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changeset | 41 envC = topEnvC, | 
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changeset | 42 txtC = NA) { ## management of warning and error messages | 
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changeset | 43 | 
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changeset | 44 tesL <- eval(parse(text = tesC), envir = envC) | 
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changeset | 45 | 
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changeset | 46 if(!tesL) { | 
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changeset | 47 | 
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changeset | 48 #sink(NULL) | 
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changeset | 49 stpTxtC <- ifelse(is.na(txtC), | 
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changeset | 50 paste0(tesC, " is FALSE"), | 
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changeset | 51 txtC) | 
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changeset | 52 | 
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changeset | 53 stop(stpTxtC, | 
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changeset | 54 call. = FALSE) | 
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changeset | 55 | 
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changeset | 56 } | 
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changeset | 57 | 
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changeset | 58 } ## flgF | 
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changeset | 59 | 
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changeset | 60 | 
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changeset | 61 ## log file | 
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changeset | 62 ##--------- | 
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changeset | 63 | 
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changeset | 64 my_print <- function(x, ...) { cat(c(x, ...))} | 
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changeset | 65 | 
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changeset | 66 my_print("\nStart of the '", modNamC, "' Galaxy module call: ", | 
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changeset | 67 format(Sys.time(), "%a %d %b %Y %X"), "\n", sep="") | 
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changeset | 68 | 
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changeset | 69 ## arguments | 
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changeset | 70 ##---------- | 
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changeset | 71 | 
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changeset | 72 # files | 
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changeset | 73 | 
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changeset | 74 dataMatrix_in <- as.character(argVc["dataMatrix_in"]) | 
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changeset | 75 dataMatrix_out <- as.character(argVc["dataMatrix_out"]) | 
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changeset | 76 | 
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changeset | 77 sampleMetadata_in <- as.character(argVc["sampleMetadata_in"]) | 
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changeset | 78 sampleMetadata_out <- as.character(argVc["sampleMetadata_out"]) | 
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changeset | 79 | 
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changeset | 80 variableMetadata_in <- as.character(argVc["variableMetadata_in"]) | 
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changeset | 81 variableMetadata_out <- as.character(argVc["variableMetadata_out"]) | 
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changeset | 82 | 
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changeset | 83 # other parameters | 
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changeset | 84 | 
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changeset | 85 transformation <- as.character(argVc["transformation"]) | 
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changeset | 86 my_imputation_label <- as.character(argVc["imputation"]) | 
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changeset | 87 my_imputation_function <- if (my_imputation_label == "zero") { | 
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changeset | 88 w4m_filter_zero_imputation | 
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changeset | 89 } else if (my_imputation_label == "center") { | 
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changeset | 90 w4m_filter_median_imputation | 
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changeset | 91 } else if (my_imputation_label == "none") { | 
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changeset | 92 w4m_filter_no_imputation | 
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changeset | 93 } else { | 
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changeset | 94 stop(sprintf("Unknown value %s supplied for 'imputation' parameter. Expected one of {zero,center,none}.")) | 
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changeset | 95 } | 
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changeset | 96 wildcards <- as.logical(argVc["wildcards"]) | 
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changeset | 97 sampleclassNames <- as.character(argVc["sampleclassNames"]) | 
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changeset | 98 sampleclassNames <- strsplit(x = sampleclassNames, split = ",", fixed = TRUE)[[1]] | 
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changeset | 99 if (wildcards) { | 
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changeset | 100 sampleclassNames <- gsub("[.]", "[.]", sampleclassNames) | 
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changeset | 101 sampleclassNames <- utils::glob2rx(sampleclassNames, trim.tail = FALSE) | 
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changeset | 102 } | 
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changeset | 103 inclusive <- as.logical(argVc["inclusive"]) | 
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changeset | 104 classnameColumn <- as.character(argVc["classnameColumn"]) | 
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changeset | 105 samplenameColumn <- as.character(argVc["samplenameColumn"]) | 
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changeset | 106 | 
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changeset | 107 order_smpl <- as.character(argVc["order_smpl"]) | 
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changeset | 108 order_vrbl <- as.character(argVc["order_vrbl"]) | 
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"planemo upload for repository https://github.com/HegemanLab/w4mclassfilter_galaxy_wrapper/tree/master commit 1a5dd14c3697516bc55950bd6e46c978af7c19cf"
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changeset | 109 | 
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changeset | 110 variable_range_filter <- as.character(argVc["variable_range_filter"]) | 
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changeset | 111 variable_range_filter <- strsplit(x = variable_range_filter, split = ",", fixed = TRUE)[[1]] | 
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changeset | 112 | 
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changeset | 113 ## ----------------------------- | 
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changeset | 114 ## Transformation and imputation | 
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changeset | 115 ## ----------------------------- | 
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changeset | 116 my_transformation_and_imputation <- if (transformation == "log10") { | 
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changeset | 117 function(m) { | 
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changeset | 118 if (!is.matrix(m)) | 
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changeset | 119 stop("Cannot transform and impute data - the supplied data is not in matrix form") | 
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changeset | 120 if (nrow(m) == 0) | 
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changeset | 121 stop("Cannot transform and impute data - data matrix has no rows") | 
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changeset | 122 if (ncol(m) == 0) | 
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changeset | 123 stop("Cannot transform and impute data - data matrix has no columns") | 
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changeset | 124 suppressWarnings({ | 
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changeset | 125 # suppress warnings here since non-positive values will produce NaN's that will be fixed in the next step | 
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changeset | 126 m <- log10(m) | 
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changeset | 127 m[is.na(m)] <- NA | 
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changeset | 128 }) | 
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changeset | 129 return ( my_imputation_function(m) ) | 
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changeset | 130 } | 
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changeset | 131 } else if (transformation == "log2") { | 
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changeset | 132 function(m) { | 
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changeset | 133 if (!is.matrix(m)) | 
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changeset | 134 stop("Cannot transform and impute data - the supplied data is not in matrix form") | 
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changeset | 135 if (nrow(m) == 0) | 
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changeset | 136 stop("Cannot transform and impute data - data matrix has no rows") | 
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changeset | 137 if (ncol(m) == 0) | 
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changeset | 138 stop("Cannot transform and impute data - data matrix has no columns") | 
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changeset | 139 suppressWarnings({ | 
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changeset | 140 # suppress warnings here since non-positive values will produce NaN's that will be fixed in the next step | 
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changeset | 141 m <- log2(m) | 
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changeset | 142 m[is.na(m)] <- NA | 
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changeset | 143 }) | 
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changeset | 144 return ( my_imputation_function(m) ) | 
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changeset | 145 } | 
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changeset | 146 } else { | 
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changeset | 147 # use the method from the w4mclassfilter class | 
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changeset | 148 my_imputation_function | 
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changeset | 149 } | 
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changeset | 150 | 
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changeset | 151 ##------------------------------ | 
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changeset | 152 ## Computation | 
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changeset | 153 ##------------------------------ | 
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changeset | 154 | 
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changeset | 155 result <- w4m_filter_by_sample_class( | 
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changeset | 156 dataMatrix_in = dataMatrix_in | 
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changeset | 157 , sampleMetadata_in = sampleMetadata_in | 
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changeset | 158 , variableMetadata_in = variableMetadata_in | 
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changeset | 159 , dataMatrix_out = dataMatrix_out | 
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changeset | 160 , sampleMetadata_out = sampleMetadata_out | 
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changeset | 161 , variableMetadata_out = variableMetadata_out | 
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changeset | 162 , classes = sampleclassNames | 
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changeset | 163 , include = inclusive | 
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changeset | 164 , class_column = classnameColumn | 
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changeset | 165 , samplename_column = samplenameColumn | 
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changeset | 166 , order_vrbl = order_vrbl | 
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changeset | 167 , order_smpl = order_smpl | 
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changeset | 168 , variable_range_filter = variable_range_filter | 
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changeset | 169 , failure_action = my_print | 
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changeset | 170 , data_imputation = my_transformation_and_imputation | 
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changeset | 171 ) | 
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changeset | 172 | 
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changeset | 173 my_print("\nResult of '", modNamC, "' Galaxy module call to 'w4mclassfilter::w4m_filter_by_sample_class' R function: ", | 
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changeset | 174 as.character(result), "\n", sep = "") | 
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changeset | 175 | 
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changeset | 176 ##-------- | 
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changeset | 177 ## Closing | 
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changeset | 178 ##-------- | 
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changeset | 179 | 
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changeset | 180 my_print("\nEnd of '", modNamC, "' Galaxy module call: ", | 
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changeset | 181 as.character(Sys.time()), "\n", sep = "") | 
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changeset | 182 | 
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changeset | 183 #sink() | 
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changeset | 184 | 
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changeset | 185 if (!file.exists(dataMatrix_out)) { | 
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changeset | 186 print(sprintf("ERROR %s::w4m_filter_by_sample_class - file '%s' was not created", modNamC, dataMatrix_out)) | 
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changeset | 187 }# else { print(sprintf("INFO %s::w4m_filter_by_sample_class - file '%s' was exists", modNamC, dataMatrix_out)) } | 
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changeset | 188 | 
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changeset | 189 if (!file.exists(variableMetadata_out)) { | 
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changeset | 190 print(sprintf("ERROR %s::w4m_filter_by_sample_class - file '%s' was not created", modNamC, variableMetadata_out)) | 
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changeset | 191 } # else { print(sprintf("INFO %s::w4m_filter_by_sample_class - file '%s' was exists", modNamC, variableMetadata_out)) } | 
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changeset | 192 | 
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changeset | 193 if (!file.exists(sampleMetadata_out)) { | 
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changeset | 194 print(sprintf("ERROR %s::w4m_filter_by_sample_class - file '%s' was not created", modNamC, sampleMetadata_out)) | 
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changeset | 195 } # else { print(sprintf("INFO %s::w4m_filter_by_sample_class - file '%s' was exists", modNamC, sampleMetadata_out)) } | 
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changeset | 196 | 
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changeset | 197 if( !result ) { | 
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changeset | 198 stop(sprintf("ERROR %s::w4m_filter_by_sample_class - method failed", modNamC)) | 
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changeset | 199 } | 
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changeset | 200 | 
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changeset | 201 rm(list = ls()) | 
