Mercurial > repos > fastaptamer > fastaptamer_compare
changeset 0:5d0073cbe61e draft
Uploaded
| author | fastaptamer |
|---|---|
| date | Tue, 10 Feb 2015 14:27:51 -0500 |
| parents | |
| children | 4795b8673e75 |
| files | fastaptamer_compare |
| diffstat | 1 files changed, 417 insertions(+), 0 deletions(-) [+] |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fastaptamer_compare Tue Feb 10 14:27:51 2015 -0500 @@ -0,0 +1,417 @@ +#!/usr/bin/env perl + +## Last Modified January 19th, 2015 22:22 CST + +## Citation: +## Khalid K. Alam, Jonathan L. Chang & Donald H. Burke. +## "FASTAptamer: A Bioinformatic Toolkit for High-Throughput Sequence Analysis of +## Combinatorial Selections." Molecular Therapy — Nucleic Acids. 2015. +## DOI: 10.1038/mtna.2015.4 + +## Distributed under GNU General Public License v3 + +use Getopt::Long; ## Core Perl module for command line options/arguments + +my $fileX_fh; ## Variable for input file X +my $fileY_fh; ## Variable for input file Y +my $output_fh; ## Variable for output file +my $help; ## true/false variable for help screen +my $all; ## true/false variable for displaying all values +my $version; ## true/false variable for displaying help screen + + ## Take command line arguments for... +GetOptions ( "X_file=s" => \$fileX_fh, ## ... input file X + "Y_file=s" => \$fileY_fh, ## ... input file Y + "output=s" => \$output_fh, ## ... output file + "help" => \$help, ## ... help screen + "quiet" => \$quiet, ## ... supress standard output + "version" => \$version, ## ... version screen + "all" => \$all); ## ... displaying all sequences + +if (defined $help){ ## Prints help screen if $help returns as true + print <<"HELP"; + +-------------------------------------------------------------------------------- + FASTAptamer-Compare +-------------------------------------------------------------------------------- + +Usage: fastaptamer_compare [-h] [-x INFILE] [-y INFILE] [-o OUTFILE] [-q] [-a] + [-v] + + [-h] = Help screen. + [-x INFILE] = Input file (from FASTAptamer-Count). REQUIRED. + [-y INFILE] = Input file (from FASTAptamer-Count). REQUIRED. + [-o OUTFILE] = Plain text output file with tab separated values. REQUIRED + [-q] = Quiet mode. Suppresses standard output of file I/O + and execution time. + [-a] = Output all sequences, including those present in only + one input file. Default behavior suppresses output + of sequences without a match. + [-v] = Display version. + +FASTAptamer-Compare facilitates statistical analysis of two populations by rapi- +dly generating a tab-delimited output file that lists each unique sequence along +with RPM (reads per million) in each population file (if available) and log(bas- +e 2) of the ratio of their RPM values in each population. + +RPM data for both populations can be utilized to generate an XY-scatter plot of +sequence distribution across two populations. FASTAptamer-Compare also facilit- +ates the generation of a histogram of the sequence distribution by creating 102 +bins for the log(base2) values. This histogram can provide a quick visual comp- +arison of the two populations: distributions centered around 0 indicate similar +populations, while distributions shifted to the left or right indicate overall +enrichment or depletion. + +Input for FASTAptamer-Compare MUST come from FASTAptamer-Count output files. + +HELP +exit; +} + +if (defined $version){ ## Print version screen if -v is true + print <<"VERSION"; + +FASTAptamer v1.0.2 + +VERSION +exit; +} + +########################################## +## Open input file or exit with warning # +########################################## + +open X_file, '<', $fileX_fh or die +"\nCould not open input file x, or no input file was specified.\n +See help documentation [-h], README, or User's Guide for program usage.\n"; + +########################################## +## Open input file or exit with warning # +########################################## + +open Y_file, '<', $fileY_fh or die +"\nCould not open input file y, or no input file was specified.\n +See help documentation [-h], README, or User's Guide for program usage.\n"; + +########################################## +## Open output file or exit with warning # +########################################## + +open OUTPUT, '>', $output_fh or die +"\nCould not create output file or no output file was specified.\n +See help documentation [-h], README, or User's Guide for program usage.\n"; + +$/ = ">"; ## Change default input record separator to read FASTA formatted file + +my $start = time; ## Record start of run-time + +my %sequences_in_x; ## Hash variable for sequences present in input file X +my %sequences_in_y; ## Hash variable for sequences present in input file Y + +################################################ +## Variables for the creation of bin "buckets" # +################################################ + +my $bin_low=0; +my $bin_1=0; my $bin_2=0; my $bin_3=0; my $bin_4=0; +my $bin_5=0; my $bin_6=0; my $bin_7=0; my $bin_8=0; +my $bin_9=0; my $bin_10=0; my $bin_11=0; my $bin_12=0; +my $bin_13=0; my $bin_14=0; my $bin_15=0; my $bin_16=0; +my $bin_17=0; my $bin_18=0; my $bin_19=0; my $bin_20=0; +my $bin_21=0; my $bin_22=0; my $bin_23=0; my $bin_24=0; +my $bin_25=0; my $bin_26=0; my $bin_27=0; my $bin_28=0; +my $bin_29=0; my $bin_30=0; my $bin_31=0; my $bin_32=0; +my $bin_33=0; my $bin_34=0; my $bin_35=0; my $bin_36=0; +my $bin_37=0; my $bin_38=0; my $bin_39=0; my $bin_40=0; +my $bin_41=0; my $bin_42=0; my $bin_43=0; my $bin_44=0; +my $bin_45=0; my $bin_46=0; my $bin_47=0; my $bin_48=0; +my $bin_49=0; my $bin_50=0; my $bin_51=0; my $bin_52=0; +my $bin_53=0; my $bin_54=0; my $bin_55=0; my $bin_56=0; +my $bin_57=0; my $bin_58=0; my $bin_59=0; my $bin_60=0; +my $bin_61=0; my $bin_62=0; my $bin_63=0; my $bin_64=0; +my $bin_65=0; my $bin_66=0; my $bin_67=0; my $bin_68=0; +my $bin_69=0; my $bin_70=0; my $bin_71=0; my $bin_72=0; +my $bin_73=0; my $bin_74=0; my $bin_75=0; my $bin_76=0; +my $bin_77=0; my $bin_78=0; my $bin_79=0; my $bin_80=0; +my $bin_81=0; my $bin_82=0; my $bin_83=0; my $bin_84=0; +my $bin_85=0; my $bin_86=0; my $bin_87=0; my $bin_88=0; +my $bin_89=0; my $bin_90=0; my $bin_91=0; my $bin_92=0; +my $bin_93=0; my $bin_94=0; my $bin_95=0; my $bin_96=0; +my $bin_97=0; my $bin_98=0; my $bin_99=0; my $bin_100=0; +my $bin_high=0; + +################################################################################ +## While loops iterate through the input files and reads each FASTA entry # +## and populates a hash where the key is the sequence and the value is the # +## reads per million for the sequence. # +################################################################################ + +while (<X_file>){ + if ($_ =~ /\d+-\d+-(\d+\.?\d*)\n(\S+)/){ + my $reads_per_million = $1; + my $sequence = $2; + $sequences_in_x{$sequence} = $reads_per_million; + } +} + +close X_file; + +while (<Y_file>){ + if ($_ =~ /\d+-\d+-(\d+\.?\d*)\n(\S+)/){ + my $reads_per_million = $1; + my $sequence = $2; + $sequences_in_y{$sequence} = $reads_per_million; + } +} + +close Y_file; + +################################################################################ + +print OUTPUT "Sequence\tRPM (x)\tRPM (y)\tlog(base2)(y/x)\n"; + +for my $sequence_key (keys %sequences_in_x){ + my $rpm_x = $sequences_in_x{$sequence_key}; + my $rpm_y = $sequences_in_y{$sequence_key}; + if ($rpm_y){ + delete $sequences_in_x{$sequence_key}; + delete $sequences_in_y{$sequence_key}; + my $ratio = $rpm_y/$rpm_x; + my $log_val = log($ratio)/log(2); + print OUTPUT "$sequence_key\t$rpm_x\t$rpm_y\t$log_val\n"; + &bin_bucket ($log_val); + } +} + +if (defined $all){ + for my $sequence_key (keys %sequences_in_x){ + my $rpm_a = $sequences_in_x{$sequence_key}; + delete $sequences_in_x{$sequence_key}; + print OUTPUT "$sequence_key\t$rpm_a\n"; + } + + for my $sequence_key (keys %sequences_in_y){ + my $rpm_b = $sequences_in_y{$sequence_key}; + delete $sequences_in_y{$sequence_key}; + print OUTPUT "$sequence_key\t\t$rpm_b\n"; + } +} + +print OUTPUT "\n\nHISTOGRAM DATA\nBin\tFrequency\n"; +print OUTPUT "\< -5\t$bin_low\n"; +print OUTPUT "-4.9\t$bin_1\n"; +print OUTPUT "-4.8\t$bin_2\n"; +print OUTPUT "-4.7\t$bin_3\n"; +print OUTPUT "-4.6\t$bin_4\n"; +print OUTPUT "-4.5\t$bin_5\n"; +print OUTPUT "-4.4\t$bin_6\n"; +print OUTPUT "-4.3\t$bin_7\n"; +print OUTPUT "-4.2\t$bin_8\n"; +print OUTPUT "-4.1\t$bin_9\n"; +print OUTPUT "-4.0\t$bin_10\n"; +print OUTPUT "-3.9\t$bin_11\n"; +print OUTPUT "-3.8\t$bin_12\n"; +print OUTPUT "-3.7\t$bin_13\n"; +print OUTPUT "-3.6\t$bin_14\n"; +print OUTPUT "-3.5\t$bin_15\n"; +print OUTPUT "-3.4\t$bin_16\n"; +print OUTPUT "-3.3\t$bin_17\n"; +print OUTPUT "-3.2\t$bin_18\n"; +print OUTPUT "-3.1\t$bin_19\n"; +print OUTPUT "-3.0\t$bin_20\n"; +print OUTPUT "-2.9\t$bin_21\n"; +print OUTPUT "-2.8\t$bin_22\n"; +print OUTPUT "-2.7\t$bin_23\n"; +print OUTPUT "-2.6\t$bin_24\n"; +print OUTPUT "-2.5\t$bin_25\n"; +print OUTPUT "-2.4\t$bin_26\n"; +print OUTPUT "-2.3\t$bin_27\n"; +print OUTPUT "-2.2\t$bin_28\n"; +print OUTPUT "-2.1\t$bin_29\n"; +print OUTPUT "-2.0\t$bin_30\n"; +print OUTPUT "-1.9\t$bin_31\n"; +print OUTPUT "-1.8\t$bin_32\n"; +print OUTPUT "-1.7\t$bin_33\n"; +print OUTPUT "-1.6\t$bin_34\n"; +print OUTPUT "-1.5\t$bin_35\n"; +print OUTPUT "-1.4\t$bin_36\n"; +print OUTPUT "-1.3\t$bin_37\n"; +print OUTPUT "-1.2\t$bin_38\n"; +print OUTPUT "-1.1\t$bin_39\n"; +print OUTPUT "-1.0\t$bin_40\n"; +print OUTPUT "-0.9\t$bin_41\n"; +print OUTPUT "-0.8\t$bin_42\n"; +print OUTPUT "-0.7\t$bin_43\n"; +print OUTPUT "-0.6\t$bin_44\n"; +print OUTPUT "-0.5\t$bin_45\n"; +print OUTPUT "-0.4\t$bin_46\n"; +print OUTPUT "-0.3\t$bin_47\n"; +print OUTPUT "-0.2\t$bin_48\n"; +print OUTPUT "-0.1\t$bin_49\n"; +print OUTPUT "0.0\t$bin_50\n"; +print OUTPUT "0.1\t$bin_51\n"; +print OUTPUT "0.2\t$bin_52\n"; +print OUTPUT "0.3\t$bin_53\n"; +print OUTPUT "0.4\t$bin_54\n"; +print OUTPUT "0.5\t$bin_55\n"; +print OUTPUT "0.6\t$bin_56\n"; +print OUTPUT "0.7\t$bin_57\n"; +print OUTPUT "0.8\t$bin_58\n"; +print OUTPUT "0.9\t$bin_59\n"; +print OUTPUT "1.0\t$bin_60\n"; +print OUTPUT "1.1\t$bin_61\n"; +print OUTPUT "1.2\t$bin_62\n"; +print OUTPUT "1.3\t$bin_63\n"; +print OUTPUT "1.4\t$bin_64\n"; +print OUTPUT "1.5\t$bin_65\n"; +print OUTPUT "1.6\t$bin_66\n"; +print OUTPUT "1.7\t$bin_67\n"; +print OUTPUT "1.8\t$bin_68\n"; +print OUTPUT "1.9\t$bin_69\n"; +print OUTPUT "2.0\t$bin_70\n"; +print OUTPUT "2.1\t$bin_71\n"; +print OUTPUT "2.2\t$bin_72\n"; +print OUTPUT "2.3\t$bin_73\n"; +print OUTPUT "2.4\t$bin_74\n"; +print OUTPUT "2.5\t$bin_75\n"; +print OUTPUT "2.6\t$bin_76\n"; +print OUTPUT "2.7\t$bin_77\n"; +print OUTPUT "2.8\t$bin_78\n"; +print OUTPUT "2.9\t$bin_79\n"; +print OUTPUT "3.0\t$bin_80\n"; +print OUTPUT "3.1\t$bin_81\n"; +print OUTPUT "3.2\t$bin_82\n"; +print OUTPUT "3.3\t$bin_83\n"; +print OUTPUT "3.4\t$bin_84\n"; +print OUTPUT "3.5\t$bin_85\n"; +print OUTPUT "3.6\t$bin_86\n"; +print OUTPUT "3.7\t$bin_87\n"; +print OUTPUT "3.8\t$bin_88\n"; +print OUTPUT "3.9\t$bin_89\n"; +print OUTPUT "4.0\t$bin_90\n"; +print OUTPUT "4.1\t$bin_91\n"; +print OUTPUT "4.2\t$bin_92\n"; +print OUTPUT "4.3\t$bin_93\n"; +print OUTPUT "4.4\t$bin_94\n"; +print OUTPUT "4.5\t$bin_95\n"; +print OUTPUT "4.6\t$bin_96\n"; +print OUTPUT "4.7\t$bin_97\n"; +print OUTPUT "4.8\t$bin_98\n"; +print OUTPUT "4.9\t$bin_99\n"; +print OUTPUT "5.0\t$bin_100\n"; +print OUTPUT "\> 5\t$bin_low\n"; + +my $duration = time - $start; + +unless (defined $quiet){ + print "\nInput file (x): \"$fileX_fh\".\nInput file (y): \"$fileY_fh\".\n"; + print "Output file: \"$output_fh\".\n"; + print "Execution time: $duration s.\n"; +} + + +sub bin_bucket{ + my $log_val = $_[0]; + if ($log_val <= -5) { $bin_low++ ;} + elsif ( $log_val > -5 && $log_val <= -4.9 ){ $bin_1++ ;} + elsif ( $log_val > -4.9 && $log_val <= -4.8 ){ $bin_2++ ;} + elsif ( $log_val > -4.8 && $log_val <= -4.7 ){ $bin_3++ ;} + elsif ( $log_val > -4.7 && $log_val <= -4.6 ){ $bin_4++ ;} + elsif ( $log_val > -4.6 && $log_val <= -4.5 ){ $bin_5++ ;} + elsif ( $log_val > -4.5 && $log_val <= -4.4 ){ $bin_6++ ;} + elsif ( $log_val > -4.4 && $log_val <= -4.3 ){ $bin_7++ ;} + elsif ( $log_val > -4.3 && $log_val <= -4.2 ){ $bin_8++ ;} + elsif ( $log_val > -4.2 && $log_val <= -4.1 ){ $bin_9++ ;} + elsif ( $log_val > -4.1 && $log_val <= -4.0 ){ $bin_10++ ;} + elsif ( $log_val > -4.0 && $log_val <= -3.9 ){ $bin_11++ ;} + elsif ( $log_val > -3.9 && $log_val <= -3.8 ){ $bin_12++ ;} + elsif ( $log_val > -3.8 && $log_val <= -3.7 ){ $bin_13++ ;} + elsif ( $log_val > -3.7 && $log_val <= -3.6 ){ $bin_14++ ;} + elsif ( $log_val > -3.6 && $log_val <= -3.5 ){ $bin_15++ ;} + elsif ( $log_val > -3.5 && $log_val <= -3.4 ){ $bin_16++ ;} + elsif ( $log_val > -3.4 && $log_val <= -3.3 ){ $bin_17++ ;} + elsif ( $log_val > -3.3 && $log_val <= -3.2 ){ $bin_18++ ;} + elsif ( $log_val > -3.2 && $log_val <= -3.1 ){ $bin_19++ ;} + elsif ( $log_val > -3.1 && $log_val <= -3.0 ){ $bin_20++ ;} + elsif ( $log_val > -3.0 && $log_val <= -2.9 ){ $bin_21++ ;} + elsif ( $log_val > -2.9 && $log_val <= -2.8 ){ $bin_22++ ;} + elsif ( $log_val > -2.8 && $log_val <= -2.7 ){ $bin_23++ ;} + elsif ( $log_val > -2.7 && $log_val <= -2.6 ){ $bin_24++ ;} + elsif ( $log_val > -2.6 && $log_val <= -2.5 ){ $bin_25++ ;} + elsif ( $log_val > -2.5 && $log_val <= -2.4 ){ $bin_26++ ;} + elsif ( $log_val > -2.4 && $log_val <= -2.3 ){ $bin_27++ ;} + elsif ( $log_val > -2.3 && $log_val <= -2.2 ){ $bin_28++ ;} + elsif ( $log_val > -2.2 && $log_val <= -2.1 ){ $bin_29++ ;} + elsif ( $log_val > -2.1 && $log_val <= -2.0 ){ $bin_30++ ;} + elsif ( $log_val > -2.0 && $log_val <= -1.9 ){ $bin_31++ ;} + elsif ( $log_val > -1.9 && $log_val <= -1.8 ){ $bin_32++ ;} + elsif ( $log_val > -1.8 && $log_val <= -1.7 ){ $bin_33++ ;} + elsif ( $log_val > -1.7 && $log_val <= -1.6 ){ $bin_34++ ;} + elsif ( $log_val > -1.6 && $log_val <= -1.5 ){ $bin_35++ ;} + elsif ( $log_val > -1.5 && $log_val <= -1.4 ){ $bin_36++ ;} + elsif ( $log_val > -1.4 && $log_val <= -1.3 ){ $bin_37++ ;} + elsif ( $log_val > -1.3 && $log_val <= -1.2 ){ $bin_38++ ;} + elsif ( $log_val > -1.2 && $log_val <= -1.1 ){ $bin_39++ ;} + elsif ( $log_val > -1.1 && $log_val <= -1.0 ){ $bin_40++ ;} + elsif ( $log_val > -1.0 && $log_val <= -0.9 ){ $bin_41++ ;} + elsif ( $log_val > -0.9 && $log_val <= -0.8 ){ $bin_42++ ;} + elsif ( $log_val > -0.8 && $log_val <= -0.7 ){ $bin_43++ ;} + elsif ( $log_val > -0.7 && $log_val <= -0.6 ){ $bin_44++ ;} + elsif ( $log_val > -0.6 && $log_val <= -0.5 ){ $bin_45++ ;} + elsif ( $log_val > -0.5 && $log_val <= -0.4 ){ $bin_46++ ;} + elsif ( $log_val > -0.4 && $log_val <= -0.3 ){ $bin_47++ ;} + elsif ( $log_val > -0.3 && $log_val <= -0.2 ){ $bin_48++ ;} + elsif ( $log_val > -0.2 && $log_val <= -0.1 ){ $bin_49++ ;} + elsif ( $log_val > -0.1 && $log_val <= 0.0 ){ $bin_50++ ;} + elsif ( $log_val > 0.0 && $log_val <= 0.1 ){ $bin_51++ ;} + elsif ( $log_val > 0.1 && $log_val <= 0.2 ){ $bin_52++ ;} + elsif ( $log_val > 0.2 && $log_val <= 0.3 ){ $bin_53++ ;} + elsif ( $log_val > 0.3 && $log_val <= 0.4 ){ $bin_54++ ;} + elsif ( $log_val > 0.4 && $log_val <= 0.5 ){ $bin_55++ ;} + elsif ( $log_val > 0.5 && $log_val <= 0.6 ){ $bin_56++ ;} + elsif ( $log_val > 0.6 && $log_val <= 0.7 ){ $bin_57++ ;} + elsif ( $log_val > 0.7 && $log_val <= 0.8 ){ $bin_58++ ;} + elsif ( $log_val > 0.8 && $log_val <= 0.9 ){ $bin_59++ ;} + elsif ( $log_val > 0.9 && $log_val <= 1.0 ){ $bin_60++ ;} + elsif ( $log_val > 1.0 && $log_val <= 1.1 ){ $bin_61++ ;} + elsif ( $log_val > 1.1 && $log_val <= 1.2 ){ $bin_62++ ;} + elsif ( $log_val > 1.2 && $log_val <= 1.3 ){ $bin_63++ ;} + elsif ( $log_val > 1.3 && $log_val <= 1.4 ){ $bin_64++ ;} + elsif ( $log_val > 1.4 && $log_val <= 1.5 ){ $bin_65++ ;} + elsif ( $log_val > 1.5 && $log_val <= 1.6 ){ $bin_66++ ;} + elsif ( $log_val > 1.6 && $log_val <= 1.7 ){ $bin_67++ ;} + elsif ( $log_val > 1.7 && $log_val <= 1.8 ){ $bin_68++ ;} + elsif ( $log_val > 1.8 && $log_val <= 1.9 ){ $bin_69++ ;} + elsif ( $log_val > 1.9 && $log_val <= 2.0 ){ $bin_70++ ;} + elsif ( $log_val > 2.0 && $log_val <= 2.1 ){ $bin_71++ ;} + elsif ( $log_val > 2.1 && $log_val <= 2.2 ){ $bin_72++ ;} + elsif ( $log_val > 2.2 && $log_val <= 2.3 ){ $bin_73++ ;} + elsif ( $log_val > 2.3 && $log_val <= 2.4 ){ $bin_74++ ;} + elsif ( $log_val > 2.4 && $log_val <= 2.5 ){ $bin_75++ ;} + elsif ( $log_val > 2.5 && $log_val <= 2.6 ){ $bin_76++ ;} + elsif ( $log_val > 2.6 && $log_val <= 2.7 ){ $bin_77++ ;} + elsif ( $log_val > 2.7 && $log_val <= 2.8 ){ $bin_78++ ;} + elsif ( $log_val > 2.8 && $log_val <= 2.9 ){ $bin_79++ ;} + elsif ( $log_val > 2.9 && $log_val <= 3.0 ){ $bin_80++ ;} + elsif ( $log_val > 3.0 && $log_val <= 3.1 ){ $bin_81++ ;} + elsif ( $log_val > 3.1 && $log_val <= 3.2 ){ $bin_82++ ;} + elsif ( $log_val > 3.2 && $log_val <= 3.3 ){ $bin_83++ ;} + elsif ( $log_val > 3.3 && $log_val <= 3.4 ){ $bin_84++ ;} + elsif ( $log_val > 3.4 && $log_val <= 3.5 ){ $bin_85++ ;} + elsif ( $log_val > 3.5 && $log_val <= 3.6 ){ $bin_86++ ;} + elsif ( $log_val > 3.6 && $log_val <= 3.7 ){ $bin_87++ ;} + elsif ( $log_val > 3.7 && $log_val <= 3.8 ){ $bin_88++ ;} + elsif ( $log_val > 3.8 && $log_val <= 3.9 ){ $bin_89++ ;} + elsif ( $log_val > 3.9 && $log_val <= 4.0 ){ $bin_90++ ;} + elsif ( $log_val > 4.0 && $log_val <= 4.1 ){ $bin_91++ ;} + elsif ( $log_val > 4.1 && $log_val <= 4.2 ){ $bin_92++ ;} + elsif ( $log_val > 4.2 && $log_val <= 4.3 ){ $bin_93++ ;} + elsif ( $log_val > 4.3 && $log_val <= 4.4 ){ $bin_94++ ;} + elsif ( $log_val > 4.4 && $log_val <= 4.5 ){ $bin_95++ ;} + elsif ( $log_val > 4.5 && $log_val <= 4.6 ){ $bin_96++ ;} + elsif ( $log_val > 4.6 && $log_val <= 4.7 ){ $bin_97++ ;} + elsif ( $log_val > 4.7 && $log_val <= 4.8 ){ $bin_98++ ;} + elsif ( $log_val > 4.8 && $log_val <= 4.9 ){ $bin_99++ ;} + elsif ( $log_val > 4.9 && $log_val <= 5.0 ){ $bin_100++ ;} + elsif ( $log_val > 5) { $bin_high++ ;} +}