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1 <?xml version="1.0" ?>
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2 <tool id="qiime_demux_subsample-single" name="qiime demux subsample-single"
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3 version="2020.8">
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4 <description>Subsample single-end sequences without replacement.</description>
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5 <requirements>
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6 <requirement type="package" version="2020.8">qiime2</requirement>
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7 </requirements>
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8 <command><![CDATA[
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9 qiime demux subsample-single
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10
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11 --i-sequences=$isequences
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12
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13 --p-fraction=$pfraction
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15 --o-subsampled-sequences=osubsampledsequences
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16
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17 #if str($examples) != 'None':
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18 --examples=$examples
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19 #end if
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20
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21 ;
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22 cp osubsampledsequences.qza $osubsampledsequences
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23
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24 ]]></command>
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25 <inputs>
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26 <param format="qza,no_unzip.zip" label="--i-sequences: ARTIFACT SampleData[SequencesWithQuality | PairedEndSequencesWithQuality] The demultiplexed sequences to be subsampled. [required]" name="isequences" optional="False" type="data" />
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27 <param label="--p-fraction: PROPORTION Range(0, 1, inclusive_start=False) The fraction of sequences to retain in subsample. [required]" name="pfraction" optional="False" type="text" />
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28 <param label="--examples: Show usage examples and exit." name="examples" optional="False" type="data" />
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29
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30 </inputs>
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31
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32 <outputs>
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33 <data format="qza" label="${tool.name} on ${on_string}: subsampledsequences.qza" name="osubsampledsequences" />
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34
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35 </outputs>
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36
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37 <help><![CDATA[
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38 Subsample single-end sequences without replacement.
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39 ###############################################################
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40
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41 Generate a random subsample of single-end sequences containing
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42 approximately the fraction of input sequences specified by the fraction
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43 parameter. The number of output samples will always be equal to the number
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44 of input samples, even if some of those samples contain no sequences after
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45 subsampling.
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46
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47 Parameters
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48 ----------
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49 sequences : SampleData[SequencesWithQuality | PairedEndSequencesWithQuality]
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50 The demultiplexed sequences to be subsampled.
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51 fraction : Float % Range(0, 1, inclusive_start=False)
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52 The fraction of sequences to retain in subsample.
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53
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54 Returns
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55 -------
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56 subsampled_sequences : SampleData[SequencesWithQuality]
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57 The subsampled sequences.
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58 ]]></help>
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59 <macros>
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60 <import>qiime_citation.xml</import>
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61 </macros>
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62 <expand macro="qiime_citation"/>
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63 </tool> |
