<?xml version="1.0" ?>
<tool id="qiime_demux_subsample-paired" name="qiime demux subsample-paired"
      version="2020.8">
  <description>Subsample paired-end sequences without replacement.</description>
  <requirements>
    <requirement type="package" version="2020.8">qiime2</requirement>
  </requirements>
  <command><![CDATA[
qiime demux subsample-paired

--i-sequences=$isequences

--p-fraction=$pfraction

--o-subsampled-sequences=osubsampledsequences

#if str($examples) != 'None':
--examples=$examples
#end if

;
cp osubsampledsequences.qza $osubsampledsequences

  ]]></command>
  <inputs>
    <param format="qza,no_unzip.zip" label="--i-sequences: ARTIFACT SampleData[PairedEndSequencesWithQuality] The demultiplexed sequences to be subsampled. [required]" name="isequences" optional="False" type="data" />
    <param label="--p-fraction: PROPORTION Range(0, 1, inclusive_start=False) The fraction of sequences to retain in subsample. [required]" name="pfraction" optional="False" type="text" />
    <param label="--examples: Show usage examples and exit." name="examples" optional="False" type="data" />
    
  </inputs>

  <outputs>
    <data format="qza" label="${tool.name} on ${on_string}: subsampledsequences.qza" name="osubsampledsequences" />
    
  </outputs>

  <help><![CDATA[
Subsample paired-end sequences without replacement.
###############################################################

Generate a random subsample of paired-end sequences containing
approximately the fraction of input sequences specified by the fraction
parameter. The number of output samples will always be equal to the number
of input samples, even if some of those samples contain no sequences after
subsampling.

Parameters
----------
sequences : SampleData[PairedEndSequencesWithQuality]
    The demultiplexed sequences to be subsampled.
fraction : Float % Range(0, 1, inclusive_start=False)
    The fraction of sequences to retain in subsample.

Returns
-------
subsampled_sequences : SampleData[PairedEndSequencesWithQuality]
    The subsampled sequences.
  ]]></help>
  <macros>
    <import>qiime_citation.xml</import>
  </macros>
  <expand macro="qiime_citation"/>
</tool>