annotate affiliation_OTU.xml @ 0:59bc96331073 draft default tip

planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
author frogs
date Thu, 28 Feb 2019 10:14:49 -0500
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59bc96331073 planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
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1 <?xml version="1.0"?>
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2 <!--
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3 # Copyright (C) 2015 INRA
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4 #
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5 # This program is free software: you can redistribute it and/or modify
59bc96331073 planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
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6 # it under the terms of the GNU General Public License as published by
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7 # the Free Software Foundation, either version 3 of the License, or
59bc96331073 planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
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8 # (at your option) any later version.
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9 #
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10 # This program is distributed in the hope that it will be useful,
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11 # but WITHOUT ANY WARRANTY; without even the implied warranty of
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12 # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
59bc96331073 planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
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13 # GNU General Public License for more details.
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14 #
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15 # You should have received a copy of the GNU General Public License
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16 # along with this program. If not, see <http://www.gnu.org/licenses/>.
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17 -->
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18 <tool id="FROGS_affiliation_OTU" name="FROGS Affiliation OTU" version="3.1">
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19 <description>Taxonomic affiliation of each OTU's seed by RDPtools and BLAST</description>
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20 <requirements>
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21 <requirement type="package" version="3.1.0">frogs</requirement>
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22 </requirements>
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23 <stdio>
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24 <exit_code range="1:" />
59bc96331073 planemo upload for repository https://github.com/geraldinepascal/FROGS-wrappers/tree/v3.1.0 commit 08296fc88e3e938c482c631bd515b3b7a0499647
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25 <exit_code range=":-1" />
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26 </stdio>
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27 <command>
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28 #set $reference_filename = str( $ref_file.fields.path )
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29 affiliation_OTU.py
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30 --reference "${reference_filename}"
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31 --input-biom $biom_abundance
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32 --input-fasta $fasta_sequences
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33 --output-biom $biom_affiliation
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34 --summary $summary
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35 --nb-cpus \${GALAXY_SLOTS:-1}
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36 --java-mem $mem
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37 #if $rdp
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38 --rdp
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39 #end if
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40 </command>
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41 <inputs>
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42 <!-- JOB Parameter -->
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43 <param name="mem" type="hidden" label="Memory allocation" help="The number of Go to allocation for java" value="20"></param>
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44 <!-- Database Choice -->
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45 <param name="ref_file" type="select" label="Using reference database" help="Select reference from the list">
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46 <options from_data_table="frogs_db"></options>
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47 <validator type="no_options" message="A built-in database is not available" />
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48 </param>
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49 <param name="rdp" type="boolean" label="Also perform RDP assignation?" help="Taxonomy affiliation will be perform thanks to Blast. This option allow you to perform it also with RDP classifier (default No)" />
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50 <!-- Files -->
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51 <param format="fasta" name="fasta_sequences" type="data" label="OTU seed sequence" help="OTU sequences (format: fasta)."/>
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52 <param format="biom1" name="biom_abundance" type="data" label="Abundance file" help="OTU abundances (format: BIOM)."/>
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53 </inputs>
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54 <outputs>
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55 <data format="biom1" name="biom_affiliation" label="${tool.name}: affiliation.biom" from_work_dir="affiliation.biom" />
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56 <data format="html" name="summary" label="${tool.name}: report.html" from_work_dir="report.html"/>
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57 </outputs>
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58 <tests>
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59 <test>
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60 <param name="ref_file" value="ITS1_test"/>
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61 <param name="fasta_sequences" value="references/04-filters.fasta"/>
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62 <param name="biom_abundance" value="references/04-filters.biom"/>
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63 <!-- <param name="fasta_sequences" value="swarm.fasta"/>
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64 <param name="biom_abundance" value="swarm.biom"/> -->
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65 <output name="biom_affiliation" file="references/06-affiliation.biom"/>
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66 <output name="summary" file="references/06-affiliation.html" compare="sim_size" delta="0" />
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67 </test>
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68 </tests>
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69
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70 <help>
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71
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72 .. image:: static/images/frogs_images/FROGS_logo.png
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73 :height: 144
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74 :width: 110
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75
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76
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77 .. class:: infomark page-header h2
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78
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79 What it does
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80
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81 Add taxonomic affiliation in abundance file.
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82
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83
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84 .. class:: infomark page-header h2
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85
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86 Inputs/outputs
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87
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88 .. class:: h3
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89
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90 Inputs
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91
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92 **Sequence file**:
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93
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94 The sequences (format `FASTA &lt;https://en.wikipedia.org/wiki/FASTA_format&gt;`_).
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95
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96 **Abundance file**:
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97
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98 The abundance of each OTU in each sample (format `BIOM &lt;http://biom-format.org/&gt;`_).
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99
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100 .. class:: h3
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101
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102 Outputs
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103
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104 **Abundance file** (tax_affiliation.biom):
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105
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106 The abundance file with affiliation (format `BIOM &lt;http://biom-format.org/&gt;`_).
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107
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108 **Summary file** (report.html):
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109
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110 This file presents the number of sequences affiliated by blast, and the number of multi-affiliation (format `HTML &lt;https://en.wikipedia.org/wiki/HTML&gt;`_).
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111
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112 .. image:: static/images/frogs_images/FROGS_affiliation_summary.png
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113 :height: 800
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114 :width: 600
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115
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116
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117 .. class:: infomark page-header h2
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118
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119 Reference database
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120
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121 All the databases we format (on demand) for RDPClassifier and NCBI Blast+ are inventoried here: http://genoweb.toulouse.inra.fr/frogs_databanks/assignation/readme.txt
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122
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123 .. class:: infomark page-header h2
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124
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125 How it works
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126
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127 .. csv-table::
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128 :header: "Steps", "Description"
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129 :widths: 5, 150
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130 :class: table table-striped
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131
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132 "1", "`RDPClassifier &lt;https://rdp.cme.msu.edu/tutorials/classifier/RDPtutorial_RDP_classifier.html&gt;`_ may be used with database to associate to each OTU a taxonomy and a bootstrap (example: *Bacteria;(1.0);Firmicutes;(1.0);Clostridia;(1.0);Clostridiales;(1.0);Clostridiaceae 1;(1.0);Clostridium sensu stricto;(1.0);*)."
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133 "2", "`blastn+ &lt;http://blast.ncbi.nlm.nih.gov/&gt;`_ or `needlall &lt;http://emboss.sourceforge.net/apps/release/6.6/emboss/apps/needleall.html&gt;`_ is used to find alignment between each OTU and the database. Only the bests hits with the same score are reported. blastn+ is used for merged read pair, and needall is used for artificially combined sequence. For each alignment returned, several metrics are computed: identity percentage, coverage percentage, and alignment length"
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134 "3", "For each OTU with several blastn+/needlall alignment results a consensus is determined on each taxonomic level. If all the taxa in a taxonomic rank are identical the taxon name is reported otherwise *Multi-affiliation* is reported. By example, if you have an OTU with two corresponding sequences, the first is a *Bacteria;Proteobacteria;Gamma Proteobacteria;Enterobacteriales*, the second is a *Bacteria;Proteobacteria;Beta Proteobacteria;Methylophilales*, the consensus will be *Bacteria;Proteobacteria;Multi-affiliation;Multi-affiliation*."
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135
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136 .. class:: infomark page-header h2
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137
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138 Alignment metrics details on identity percentage calculation
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139
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140 With classicala %id computation method, we will obtain for overlapped amplicon sequence and for artificially combined amplicon sequence :
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141
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142 * **Case 1: a sequencing of overlapping sequences i.e. 16S V3-V4 amplicon MiSeq sequencing**
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143
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144 .. image:: static/images/frogs_images/FROGS_affiliation_overlapped_percent_id.png
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145 :height: 325
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146 :width: 807
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147
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148 * **Case 2 : a sequencing of non-overlapping sequences: case of ITS1 amplicon MiSeq sequencing**
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149
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150 .. image:: static/images/frogs_images/FROGS_affiliation_combined_percent_id.png
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151 :height: 310
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152 :width: 887
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153
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154 * **Finally, how percentage identity is computed ?**
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155
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156 With the classical method of %id calculation, filtering on %id will systematically removed “FROGS combined” OTUs. So, we proposed to replace the classical %id by a %id computed on the sequenced bases only.
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157
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158 .. image:: static/images/frogs_images/FROGS_affiliation_percent_id_formula.png
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159 :height: 36
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160 :width: 637
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161
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162 For the precedent use cases we will obtain:
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163
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164 * Case 1: 16S V3V4 overlapped sequence
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165 % sequenced bases identity = 400 matches / 400 bp = 100%
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166
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167 * Case 2: very large ITS1 “FROGS combined” shorter than the real sequence
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168 % sequenced bases identity = (250 + 250 ) / (600 - 100) = 100%
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169
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170 This calculation allows to return 100% of identity on sequenced bases for “FROGS combined” shorter or longer than reality in case of perfect sequencing, and a smaller percentage of identity in the case of small overlap repeat kept in FROGS combined sequence.
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171
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172 .. class:: infomark page-header h2
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173
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174
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175 Advices
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177 This tool can take large time. It is recommended to filter your abundance and your sequence file before this tool (see **FROGS Filters**).
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179 As you can see the affiliation of each OTU is not human readable in outputed abundance file. We provide a tools to convert these BIOM file in tabulated file, see the **FROGS BIOM to TSV** tool.
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180
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182 ----
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183
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184 **Contact**
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185
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186 Contacts: frogs@inra.fr
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187
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188 Repository: https://github.com/geraldinepascal/FROGS
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189 website: http://frogs.toulouse.inra.fr/
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191 Please cite the **FROGS article**: *Escudie F., et al. Bioinformatics, 2018. FROGS: Find, Rapidly, OTUs with Galaxy Solution.*
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193 </help>
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194 <citations>
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195 <citation type="doi">10.1093/bioinformatics/btx791</citation>
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196 </citations>
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198 </tool>