annotate autogenJB2.xml @ 19:bde6b1d09f7d draft

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date Tue, 30 Jan 2024 06:05:03 +0000
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1 <tool id="autogenjb2" name="autogenjb2" version="2.10.0_0" profile="22.05">
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2 <description>Files to JBrowse2</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="edamInc"/>
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7 <xrefs>
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8 <xref type="bio.tools">jbrowse2</xref>
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9 </xrefs>
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10 <expand macro="requirements"/>
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11 <version_command>python '${__tool_directory__}/autogenJB2.py' --version</version_command>
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12 <command detect_errors="aggressive"><![CDATA[
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13 python '$__tool_directory__/autogenJB2.py'
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14 --outdir '$jbrowseme'
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16 &&
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18 cp '$output.files_path/index.html' '$output'
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19
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20 ## Ugly testing hack since I cannot get <extra_files> to test the files I want to test. Hmph.
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21 #if str($uglyTestingHack) == "enabled":
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22 && cp '$trackxml' '$output'
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23 #end if
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24 ]]></command>
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25 <inputs>
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26 <param
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27 label="Collection of files specially named to become tracks"
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28 name="jbrowseme"
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29 type="data_collection">
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30 </param>
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31 <param type="hidden" name="uglyTestingHack" value="" />
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32 </inputs>
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33 <outputs>
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34 <data format="html" name="output" label="JBrowse2 on $reference_genome.genome.element_identifier"/>
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35 </outputs>
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36
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37 <help><![CDATA[
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38
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39 JBrowse2-in-Galaxy
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40 ==================
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41
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42 JBrowse2-in-Galaxy offers a highly configurable, workflow-compatible
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43 alternative to JBrowse1-in-Galaxy and Trackster.
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44
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45 Compared to JBrowse1-in-Galaxy, there is no support for alternative codons for unusual genomes,
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46 and detailed track styling is not yet implemented. Send code.
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47 JBrowse1 development has now ceased in favour of JBrowse2.
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48
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49 Use and local viewing
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50 =====================
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51
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52
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53 A JBrowse2 history item can be opened by viewing it (the "eye" icon).
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54
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55 The same browser data and setup can also be downloaded as a compressed zip archive by clicking the download ("floppy disk") icon in the history.
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56 This can be shared and viewed without Galaxy.
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57
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58 A replacement application to serve the browser is required without Galaxy. A local python web server can be started using a script included in each archive,
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59 assuming that Python3 is already working on your desktop - if not you will have to install it first. Unzip the archive (*unzip [filename].zip*) and change
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60 directory to the first level in that zip archive. It contains a file named *jb2_webserver.py*
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61
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62 With python3 installed,
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63
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64 *python3 jb2_webserver.py*
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65
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66 will serve the unarchived JBrowse2 configuration from the same directory as the python script automatically. If a new browser window does not open,
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67 but the script appears to be running, try pointing your web browser to the default of *localhost:8080*
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68
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69 Overview
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70 --------
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71
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72 JBrowse is a fast, embeddable genome browser built completely with
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73 JavaScript and HTML5.
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74
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75 The JBrowse-in-Galaxy (JiG) tool was written to help build complex
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76 JBrowse installations straight from Galaxy. It allows you to build up a JBrowse instance without worrying
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77 about how to run the command line tools to format your data, and which
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78 options need to be supplied and where.
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79
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80 The JBrowse-in-Galaxy tool has been rejected by `a Galaxy IUC
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81 <https://github.com/galaxyproject/tools-iuc/issues>`__, reviewer.
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82 It is maintained by https://github.com/fubar2 who you can help you
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83 with missing features or bugs in the tool. For the record, he remains unconvinced by the reviewer's logic,
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84 and disturbed by the distinctly coercive approach to introducing new code,
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85 compared to the more usual method of providing a working PR.
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86
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87 Options
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88 -------
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89
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90 **Reference or Assembly**
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91
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92 Choose either a built-in or select one from your history.
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93
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94 Track coordinates and contig names *must* match this reference precisely
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95 or they will not display.
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96
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97 **Track Groups** represent a set of tracks in a single category.
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98
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99 Annotation Tracks
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100 -----------------
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101
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102 GFF3/BED
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103 ~~~~~~~~
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104
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105 Standard feature tracks. They usually highlight genes, mRNAs and other features of interest along a genomic region.
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106
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107 When these contain tens of millions of features, such as repeat regions from a VGP assembly, displaying one at a time leads
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108 to extremely slow loading times when a large region is in view, unless the "LinearPileupDisplay" display option is
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109 selected for that track in the styling options section. The default is LinearBasicDisplay, which shows all details and works
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110 well for relatively sparse bed files. A better option is to make a bigwig track using a set of windows based on the
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111 lengths of each assembly or reference contig.
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112
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113 BAM Pileups
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114 ~~~~~~~~~~~
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115
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116 We support BAM files and can automatically generate SNP tracks based on
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117 that bam data.
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118
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119
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120 BlastXML
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121 ~~~~~~~~
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122
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123 JiG now supports both blastn and blastp datasets. JiG internally uses a
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124 blastXML to gapped GFF3 tool to convert your blastxml datasets into a
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125 format amenable to visualization in JBrowse. This tool is also
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126 available separately from the IUC on the toolshed.
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127
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128 **Minimum Gap Size** reflects how long a gap must be before it becomes a
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129 real gap in the processed gff3 file. In the picture above, various sizes
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130 of gaps can be seen. If the minimum gap size was set much higher, say
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131 100nt, many of the smaller gaps would disappear, and the features on
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132 both sides would be merged into one, longer feature. This setting is
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133 inversely proportional to runtime and output file size. *Do not set this
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134 to a low value for large datasets*. By setting this number lower, you
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135 will have extremely large outputs and extremely long runtimes. The
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136 default was configured based off of the author's experience, but the
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137 author only works on small viruses. It is *strongly* recommended that
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138 you filter your blast results before display, e.g. picking out the top
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139 10 hits or so.
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140
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141 **Protein blast search** option merely informs underlying tools that
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142 they should adjust feature locations by 3x.
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143
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144
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145 @ATTRIBUTION@
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146 ]]></help>
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147 <expand macro="citations"/>
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148 </tool>