Mercurial > repos > gaelcge > cellranger_atac_galaxy
annotate tool_setup.md @ 0:a62bafb1aaf8 draft default tip
"planemo upload commit ff0d185541dc4b0a5839fca166f1beb459dee61c"
author | gaelcge |
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date | Tue, 02 Aug 2022 19:42:44 +0000 |
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1 # Setup for creating a Galaxy wrapper for Cellranger-ATAC with planemo |
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2 |
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3 Make sure you have a working planemo installation. To find out how to install planemo, check here: https://planemo.readthedocs.io/en/latest/installation.html |
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4 |
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5 ## Generate galaxy tool xml using planemo tool_init |
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6 planemo tool_init --force \ |
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7 --id 'cellranger_atac' \ |
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8 --name 'Process 10x Genomics single-cell ATAC-seq data using CellRanger-ATAC.'\ |
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9 --example_command 'cellranger-atac count' \ |
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10 --cite_url 'https://support.10xgenomics.com/single-cell-atac/software/pipelines/latest/using/fastq-input#ATACIntro' \ |
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11 --help_from_command 'cellranger-atac' |
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12 |
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13 ## Test run with very small test data |
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14 cellranger-atac count --id=sample345 \ |
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15 --reference= \ |
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16 --fastqs=/home/florian/GenAP2/test_datasets/cellranger_ATAC/atac_pbmc_500_nextgem_fastqs/100k_sub \ |
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17 --sample=test \ |
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18 --localcores=2 \ |
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19 --localmem=6 |