Mercurial > repos > galaxyp > custom_pro_db_annotation_data_manager
annotate data_manager/customProDB_annotation.R @ 0:45755942ae7b draft
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
author | galaxyp |
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date | Tue, 14 Mar 2017 14:11:55 -0400 |
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children | 9b4ee836e35b |
rev | line source |
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0
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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1 #!/usr/bin/env Rscript |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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2 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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3 initial.options <- commandArgs(trailingOnly = FALSE) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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4 script_parent_dir <- dirname(sub("--file=", "", initial.options[grep("--file=", initial.options)])) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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5 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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6 ## begin warning handler |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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7 withCallingHandlers({ |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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8 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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9 library(methods) # Because Rscript does not always do this |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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10 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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11 options('useFancyQuotes' = FALSE) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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12 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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13 suppressPackageStartupMessages(library("optparse")) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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14 suppressPackageStartupMessages(library("RGalaxy")) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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15 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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16 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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17 option_list <- list() |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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18 option_list$dbkey <- make_option('--dbkey', type='character') |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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19 option_list$dbsnp <- make_option('--dbsnp', type='character') |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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20 option_list$cosmic <- make_option('--cosmic', type='logical') |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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21 option_list$outputFile <- make_option('--outputFile', type='character') |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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22 option_list$dbkey_description <- make_option('--dbkey_description', type='character') |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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23 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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24 opt <- parse_args(OptionParser(option_list=option_list)) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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25 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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26 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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27 customProDB_annotation <- function( |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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28 dbkey = GalaxyCharacterParam(required=TRUE), |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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29 dbsnp_str = GalaxyCharacterParam(required=FALSE), |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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30 cosmic = GalaxyLogicalParam(required=FALSE), |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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31 dbkey_description = GalaxyCharacterParam(required=FALSE), |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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32 outputFile = GalaxyOutput("output","json")) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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33 { |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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34 if (!file.exists(outputFile)) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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35 { |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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36 gstop("json params file does not exist") |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
galaxyp
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37 } |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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38 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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39 if (length(dbkey_description) < 1) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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40 { |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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41 dbkey_description = dbkey |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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42 } |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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43 |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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44 if (length(dbsnp_str) > 0) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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45 { |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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46 dbsnp = dbsnp_str |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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47 } |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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48 else |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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49 { |
45755942ae7b
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50 dbsnp = NULL |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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51 } |
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52 |
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53 use_cosmic = FALSE |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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54 if (length(cosmic) > 0) |
45755942ae7b
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55 { |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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56 if (grepl("^hg", dbkey)) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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57 { |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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58 use_cosmic = TRUE |
45755942ae7b
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59 } |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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60 else |
45755942ae7b
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61 { |
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62 gstop("COSMIC annotation requested but dbkey does not indicate a human genome (e.g. hg19)") |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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63 } |
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64 } |
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65 |
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66 suppressPackageStartupMessages(library(rjson)) |
45755942ae7b
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/custom_pro_db commit 4bb5b663989d5f04e8fb74b111456f16d6edaa66
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67 params = fromJSON(file=outputFile) |
45755942ae7b
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68 target_directory = params$output_data[[1]]$extra_files_path |
45755942ae7b
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69 dir.create(target_directory) |
45755942ae7b
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70 |
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71 tryCatch( |
45755942ae7b
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72 { |
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73 file.remove(outputFile) |
45755942ae7b
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74 }, error=function(err) |
45755942ae7b
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75 { |
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76 gstop("failed to remove json params file after reading") |
45755942ae7b
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77 }) |
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78 |
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79 ucscTableCodingFastaURL = paste("http://genome.ucsc.edu/cgi-bin/hgTables?db=", dbkey, "&hgSeq.cdsExon=on&hgSeq.granularity=gene&hgSeq.casing=exon&hgSeq.repMasking=lower&hgta_doGenomicDna=get+sequence&hgta_group=genes&hgta_track=refGene&hgta_table=refGene&hgta_regionType=genome", sep="") |
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80 ucscTableProteinFastaURL = paste("http://genome.ucsc.edu/cgi-bin/hgTables?db=", dbkey, "&hgta_geneSeqType=protein&hgta_doGenePredSequence=submit&hgta_track=refGene&hgta_table=refGene", sep="") |
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81 codingFastaFilepath = paste(target_directory, "/", dbkey, ".cds.fa", sep="") |
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82 proteinFastaFilepath = paste(target_directory, "/", dbkey, ".protein.fa", sep="") |
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83 |
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84 suppressPackageStartupMessages(library(customProDB)) |
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85 options(timeout=3600) |
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86 |
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87 cat(paste("Downloading coding FASTA from:", ucscTableCodingFastaURL, "\n")) |
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88 download.file(ucscTableCodingFastaURL, codingFastaFilepath, quiet=T, mode='wb') |
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89 |
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90 cat(paste("Downloading protein FASTA from:", ucscTableProteinFastaURL, "\n")) |
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91 download.file(ucscTableProteinFastaURL, proteinFastaFilepath, quiet=T, mode='wb') |
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92 |
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93 cat(paste("Preparing Refseq annotation files\n")) |
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94 customProDB::PrepareAnnotationRefseq(genome=dbkey, CDSfasta=codingFastaFilepath, pepfasta=proteinFastaFilepath, annotation_path=target_directory, dbsnp=dbsnp, COSMIC=use_cosmic) |
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95 |
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96 outputPath = paste(dbkey, "/customProDB", sep="") |
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97 output = list(data_tables = list()) |
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98 output[["data_tables"]][["customProDB"]]=c(path=outputPath, name=dbkey_description, dbkey=dbkey, value=dbkey) |
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99 write(toJSON(output), file=outputFile) |
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100 } |
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101 |
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102 |
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103 params <- list() |
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104 for(param in names(opt)) |
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105 { |
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106 if (!param == "help") |
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107 params[param] <- opt[param] |
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108 } |
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109 |
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110 setClass("GalaxyRemoteError", contains="character") |
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111 wrappedFunction <- function(f) |
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112 { |
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113 tryCatch(do.call(f, params), |
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114 error=function(e) new("GalaxyRemoteError", conditionMessage(e))) |
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115 } |
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116 |
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117 |
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118 suppressPackageStartupMessages(library(RGalaxy)) |
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119 do.call(customProDB_annotation, params) |
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120 |
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121 ## end warning handler |
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122 }, warning = function(w) { |
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123 cat(paste("Warning:", conditionMessage(w), "\n")) |
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124 invokeRestart("muffleWarning") |
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125 }) |