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"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot commit 3cc1eda0c28ff138ac19c103c9fbb2a4841beed9"
author galaxyp
date Wed, 26 May 2021 19:57:41 +0000
parents 39eaac8f3f42
children
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0d089906f752 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/idpassemble commit 0be0fd3f899ce64aa53e44117931fe1a7d8c52ab
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1 <?xml version="1.0"?>
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4b418a632bb0 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot commit 3cc1eda0c28ff138ac19c103c9fbb2a4841beed9"
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2 <tool id="idpassemble" name="idpAssemble" version="@VERSION@.0" profile="19.01">
0
0d089906f752 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/idpassemble commit 0be0fd3f899ce64aa53e44117931fe1a7d8c52ab
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3 <description>Merge IDPicker databases from single files into a merged database, and filters the result at PSM/spectrum/peptide/protein/gene levels.</description>
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4 <macros>
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5 <import>macros.xml</import>
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6 </macros>
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7 <expand macro="requirements" />
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8 <stdio>
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9 <exit_code range="1:" level="fatal" description="Job Failed" />
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10 <regex match="^Error:.*$" source="both" level="fatal" />
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11 </stdio>
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12 <command>
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13 <![CDATA[
0d089906f752 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/idpassemble commit 0be0fd3f899ce64aa53e44117931fe1a7d8c52ab
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14 #if len($input) < 2
0d089906f752 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/idpassemble commit 0be0fd3f899ce64aa53e44117931fe1a7d8c52ab
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15 cp '${input}' output &&
0d089906f752 planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot/idpassemble commit 0be0fd3f899ce64aa53e44117931fe1a7d8c52ab
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16 #end if
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17
8
4b418a632bb0 "planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tools/bumbershoot commit 3cc1eda0c28ff138ac19c103c9fbb2a4841beed9"
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18 export TMPDIR=\$_GALAXY_JOB_TMP_DIR &&
0
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19 idpAssemble
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20 -MaxFDRScore $MaxFDRScore
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21 -MinDistinctPeptides $filter_at_gene_level_condition.MinDistinctPeptides
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22 -MinSpectra $filter_at_gene_level_condition.MinSpectra
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23 -MinAdditionalPeptides $filter_at_gene_level_condition.MinAdditionalPeptides
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24 -MinSpectraPerDistinctMatch $MinSpectraPerDistinctMatch
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25 -MinSpectraPerDistinctPeptide $MinSpectraPerDistinctPeptide
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26 -MaxProteinGroupsPerPeptide $MaxProteinGroupsPerPeptide
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27 #if $filter_at_gene_level_condition.FilterAtGeneLevel
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28 -FilterAtGeneLevel 1
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29 #end if
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30 -SummarizeSources 1
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31
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32 #if $AssignSourceHierarchyCondition.HasAssignSourceHierarchy
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33 -AssignSourceHierarchy '$AssignSourceHierarchyCondition.AssignSourceHierarchy'
3
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34 #end if
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35
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36 #if $IsobaricSampleMappingCondition.HasIsobaricSampleMapping
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37 -IsobaricSampleMapping '$IsobaricSampleMappingCondition.IsobaricSampleMapping'
3
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38 #end if
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39
0
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40 #if len($input) > 1
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41 -MergedOutputFilepath output
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42 #for $i in $input
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43 '${i.file_name}'
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44 #end for
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45 #else
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46 output
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47 #end if
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48 ]]>
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49 </command>
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50 <inputs>
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51 <param name="input" type="data" format="idpdb" label="Input idpDB(s)" multiple="true"/>
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52 <param argument="-MaxFDRScore" type="float" label="Max FDR Score" min="0.00000001" value="0.05" help="Peptide-spectrum-matches (PSMs) with an FDR score (interpolated Q-value) higher than this will be excluded from the filtered data set." />
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53 <conditional name="filter_at_gene_level_condition">
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54 <param argument="-FilterAtGeneLevel" type="boolean" truevalue="1" falsevalue="0" label="Filter at Gene Level" help="Apply filters at the gene level (i.e. 'min distinct peptides per gene group' instead of 'min distinct peptides per protein group')"/>
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55 <when value="1">
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56 <param argument="-MinDistinctPeptides" type="integer" label="Min Distinct Peptides per Gene Group" min="1" value="2" help="Gene groups with fewer than this number of peptides will be excluded from the filtered data set." />
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57 <param argument="-MinSpectra" type="integer" label="Min Filtered Spectra per Gene Group" min="1" value="2" help="Gene groups with fewer than this number of spectra will be excluded from the filtered data set." />
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58 <param argument="-MinAdditionalPeptides" type="integer" label="Min Additional Peptides per Gene Group" min="0" value="1" help="Gene groups that are not necessary to explain the presence of at least this many extra peptides will be from the filtered data set. A value of 1 means that each gene group must explain at least 1 peptide that other gene groups do not explain." />
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59 </when>
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60 <when value="0">
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61 <param argument="-MinDistinctPeptides" type="integer" label="Min Distinct Peptides per Protein Group" min="1" value="2" help="Protein groups with fewer than this number of peptides will be excluded from the filtered data set." />
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62 <param argument="-MinSpectra" type="integer" label="Min Filtered Spectra per Protein Group" min="1" value="2" help="Protein groups with fewer than this number of spectra will be excluded from the filtered data set." />
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63 <param argument="-MinAdditionalPeptides" type="integer" label="Min Additional Peptides per Protein Group" min="0" value="1" help="Protein groups that are not necessary to explain the presence of at least this many extra peptides will be from the filtered data set. A value of 1 means that each protein group must explain at least 1 peptide that other protein groups do not explain." />
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64 </when>
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65 </conditional>
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66 <param argument="-MinSpectraPerDistinctMatch" type="integer" label="Min Filtered Spectra per Distinct Match" min="1" value="1" help="Distinct matches with fewer than this number of spectra will be excluded from the filtered data set." />
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67 <param argument="-MinSpectraPerDistinctPeptide" type="integer" label="Min Filtered Spectra per Distinct Peptide" min="1" value="1" help="Distinct peptides with fewer than this number of spectra will be excluded from the filtered data set." />
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68 <param argument="-MaxProteinGroupsPerPeptide" type="integer" label="Max Protein Groups per Distinct Peptide" min="0" value="10" help="Peptides that map to more than this number of protein groups will be excluded from the filtered data set. Highly ambiguous peptides are not very useful for quantitation." />
5
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69
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70 <conditional name="AssignSourceHierarchyCondition">
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71 <param name="HasAssignSourceHierarchy" type="boolean" truevalue="1" falsevalue="0" label="Assign sources to a hierarchy?"/>
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72 <when value="1">
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73 <param argument="-AssignSourceHierarchy" type="data" format="tabular" optional="true" label="Assign source files to groups" help="A tab-delimited file that organizes source files (e.g. individual runs in a fractionated experiment) into groups. See below for more details." />
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74 </when>
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75 <when value="0">
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76 </when>
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77 </conditional>
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78 <conditional name="IsobaricSampleMappingCondition">
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79 <param name="HasIsobaricSampleMapping" type="boolean" truevalue="1" falsevalue="0" label="Assign sample names to reporter ions?"/>
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80 <when value="1">
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81 <param argument="-IsobaricSampleMapping" type="data" format="tabular" optional="true" label="Assign sample names to reporter ions" help="A tab-delimited file that gives sample names to isobaric reporter ion channels (i.e. iTRAQ, TMT) across a given source group. See below for more details." />
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82 </when>
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83 <when value="0">
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84 </when>
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85 </conditional>
0
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86 </inputs>
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87 <outputs>
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88 <data format="idpdb" name="output" from_work_dir="output" />
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89 </outputs>
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90 <tests>
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91 <test>
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92 <param name="input" value="201208-378803-mm.idpDB" />
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93 <param name="MaxFDRScore" value="0.05" />
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94 <param name="filter_at_gene_level_condition.MinDistinctPeptides" value="2" />
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95 <param name="filter_at_gene_level_condition.MinSpectra" value="2" />
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96 <param name="filter_at_gene_level_condition.MinAdditionalPeptides" value="1" />
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97 <param name="MinSpectraPerDistinctMatch" value="1" />
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98 <param name="MinSpectraPerDistinctPeptide" value="1" />
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99 <param name="MaxProteinGroupsPerPeptide" value="10" />
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100 <output name="output" file="201208-378803-mm-filtered.idpDB" compare="sim_size" delta="500000" />
0
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101 </test>
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102 <test>
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103 <param name="input" value="201208-378803-msgf.idpDB" />
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104 <param name="MaxFDRScore" value="0.05" />
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105 <param name="filter_at_gene_level_condition.MinDistinctPeptides" value="2" />
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106 <param name="filter_at_gene_level_condition.MinSpectra" value="2" />
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107 <param name="filter_at_gene_level_condition.MinAdditionalPeptides" value="1" />
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108 <param name="MinSpectraPerDistinctMatch" value="1" />
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109 <param name="MinSpectraPerDistinctPeptide" value="1" />
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110 <param name="MaxProteinGroupsPerPeptide" value="10" />
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111 <output name="output" file="201208-378803-msgf-filtered.idpDB" compare="sim_size" delta="500000" />
0
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112 </test>
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113 <test>
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114 <param name="input" value="201208-378803-cm.idpDB" />
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115 <param name="MaxFDRScore" value="0.05" />
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116 <param name="filter_at_gene_level_condition.MinDistinctPeptides" value="2" />
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117 <param name="filter_at_gene_level_condition.MinSpectra" value="2" />
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118 <param name="filter_at_gene_level_condition.MinAdditionalPeptides" value="1" />
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119 <param name="MinSpectraPerDistinctMatch" value="1" />
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120 <param name="MinSpectraPerDistinctPeptide" value="1" />
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121 <param name="MaxProteinGroupsPerPeptide" value="10" />
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122 <param name="HasAssignSourceHierarchy" value="1" />
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123 <param name="AssignSourceHierarchy" value="assembly.tsv" ftype="tabular" />
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124 <output name="output" file="201208-378803-cm-filtered.idpDB" compare="sim_size" delta="500000" />
0
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125 </test>
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126 <test>
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127 <param name="input" value="201208-378803-mm.idpDB,201208-378803-msgf.idpDB,201208-378803-cm.idpDB" />
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128 <param name="MaxFDRScore" value="0.05" />
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129 <param name="filter_at_gene_level_condition.MinDistinctPeptides" value="2" />
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130 <param name="filter_at_gene_level_condition.MinSpectra" value="2" />
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131 <param name="filter_at_gene_level_condition.MinAdditionalPeptides" value="1" />
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132 <param name="MinSpectraPerDistinctMatch" value="1" />
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133 <param name="MinSpectraPerDistinctPeptide" value="1" />
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134 <param name="MaxProteinGroupsPerPeptide" value="10" />
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135 <output name="output" file="201208-378803.idpDB" compare="sim_size" delta="500000" />
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136 </test>
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137 <test>
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138 <param name="input" value="201208-378803-embeddedGenesAndQuantitation.idpDB" />
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139 <param name="HasAssignSourceHierarchy" value="1" />
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140 <param name="HasIsobaricSampleMapping" value="1" />
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141 <param name="IsobaricSampleMapping" value="mapping.tsv" ftype="tabular" />
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142 <param name="AssignSourceHierarchy" value="assembly.tsv" ftype="tabular" />
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143 <output name="output" file="201208-378803-embeddedGenesAndQuantitationWithMapping.idpDB" compare="sim_size" delta="500000" />
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144 </test>
0
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145 </tests>
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146 <help>
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147 <![CDATA[
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148 **What it does**
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149
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150 Merges and filters one or more IDPicker 3 idpDB files into a combined idpDB file. Protein assembly (e.g. parsimony) is conducted on the combined set of proteins.
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151
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152 ====
3
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153
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154 **AssignSourceHierarchy**
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155
3
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156 The assembly file is a tab-delimited file with two columns that organizes the sources (individual runs) into a hierarchy.
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157 The first column is the name of a source group, the second column is the source path or name to assign to that group.
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158 A forward slash in the group name adds another level to the hierarchy (just like a directory path).
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159
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160 *A simple example:*
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161
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162 ::
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163
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164 /repA repA1.idpDB
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165 /repA repA2.idpDB
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166 /repB repB1.idpDB
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167 /repB repB2.idpDB
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168
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169
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170 *A multi-level example:*
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171
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172 ::
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173
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174 /A/1 A1_f1
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175 /A/1 A1_f2
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176 /A/2 A2_f1
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177 /A/2 A2_f2
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178 /B/1 B1_f1
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179 /B/1 B1_f2
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180 /B/2 B2_f1
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181 /B/2 B2_f2
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182
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183 ====
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184
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185 **IsobaricSampleMapping**
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186
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187 The mapping file is a tab-delimited file with two columns. The first column is the full path to a source group,
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188 the second column is a comma-delimited list of sample names, in ascending order of reporter ion mass. The special
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189 sample name *Reference*, if present, will be used to normalize the other channels. Samples named *Empty* will be
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190 ignored.
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191
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192
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193 *iTRAQ-4plex example:*
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194
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195 ::
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196
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197 /Case/Group1_A123_B456_C789 A123,B456,C789,Reference
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198 /Case/Group2_D123_E456_F789 D123,E456,F789,Reference
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199 /Ctrl/Group3_X123_Y456_Z789 Reference,X123,Y456,Z789
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200 /Ctrl/Group4_U123_V456_None U123,Reference,V456,Empty
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201
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202
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203 *TMT-10plex example:*
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204
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205 ::
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206
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207 /Group1_Cases1-4_Controls1-4 Case1,Case2,Case3,Case4,Reference,Control1,Control2,Control3,Control4,Reference
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208 /Group2_Cases5-8_Controls5-8 Case5,Case6,Case7,Case8,Reference,Control5,Control6,Control7,Control8,Reference
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209
0
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210 ]]>
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211 </help>
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212 <citations>
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213 <citation type="doi">10.1021/pr900360j</citation>
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214 <citation type="bibtex">@misc{toolsGalaxyP, author = {Chilton, J, Chambers MC, et al.}, title = {Galaxy Proteomics Tools}, publisher = {GitHub}, journal = {GitHub repository},
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215 year = {2015}, url = {https://github.com/galaxyproteomics/tools-galaxyp}}</citation> <!-- TODO: fix substitution of commit ", commit = {$sha1$}" -->
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216 </citations>
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217 </tool>