Mercurial > repos > galaxyp > peptideshaker
annotate README.rst @ 55:bb0130ff73ce draft
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
author | galaxyp |
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date | Fri, 15 Jan 2021 14:06:27 +0000 |
parents | 815f93bb3e1b |
children | 1beff3ddce58 |
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2cafc729b2ae
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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1 GalaxyP - PeptideShaker |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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2 ======================= |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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3 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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4 - Home: <https://github.com/galaxyproteomics/tools-galaxyp/> |
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5 - Galaxy Tool Shed: <http://toolshed.g2.bx.psu.edu/view/galaxyp/peptideshaker> |
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"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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6 - Tools ID: `peptide_shaker`, `search_gui`, `ident_params`, `fasta_cli` |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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7 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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8 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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9 Description |
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10 ----------- |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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11 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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12 Perform protein identification combining numerous search engines (using SearchGUI) followed by peptide and protein inference with PeptideShaker. |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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13 |
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bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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14 Includes tool wrappers for FastaCLI, IdentificationParametersCLI, SearchGUI and PeptideShaker. |
bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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15 |
bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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16 |
bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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17 |
bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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18 FastaCLI adds decoy sequences to any fasta file. |
bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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19 |
bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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20 The Identification Parameters tool allows to create a parameters (par) file which can be (re)used later to parameterize SearchGUI or PeptideShaker. |
bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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21 |
bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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22 The SearchGUI tool takes any number of mgf files and performs searches on these. It creates a large zip archive with all search results, the original database and spectra. |
bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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23 |
bb0130ff73ce
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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24 This can then be fed to the PeptideShaker tool which merges the results and performs peptide and protein inference. |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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25 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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26 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 327a3944f2f56668d607c3d6cb08ae7d588e208c
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27 General Requirements |
dfaea053e32f
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 327a3944f2f56668d607c3d6cb08ae7d588e208c
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28 -------------------- |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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29 |
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"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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30 To avoid out of memory errors you should set the maximum heapspace for java processes as the default is most likely too small. For example, to set this in your shell: |
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31 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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32 export _JAVA_OPTIONS='-Xmx1500M' |
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33 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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34 On some systems you may also need to adjust the amount of memory available for class definitions in addition to the maximum heapspace. For example: |
2cafc729b2ae
planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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35 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 0e976aeeed9d516e0ad4735dc847b1f2eba3532b
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36 export _JAVA_OPTIONS='-Xmx1500M -XX:MaxPermSize=256M' |
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37 |
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38 It is also possible to set this on a per tool basis using advanced features of the galaxy job config system. |
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39 |
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40 MSAmanda on linux |
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41 ----------------- |
dfaea053e32f
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42 |
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planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit 327a3944f2f56668d607c3d6cb08ae7d588e208c
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43 Running MS Amanda on Linux requires that you have Mono installed. Mono 3.2.1 or newer is required. If you install via the toolshed Mono should be installed automatically, however if this does not work you can install it manually. |
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"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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44 |
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45 On ubuntu Mono can be installed as follows |
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46 |
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47 sudo apt-get install mono-runtime |
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48 sudo apt-get install libmono-system-core4.0-cil |
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49 |
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"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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50 For more help on installing Mono please see http://www.mono-project.com/download. |
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51 |
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52 Note |
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53 ---- |
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54 |
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"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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55 - Requires Galaxy release v16.01 or later. |
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56 |
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57 See: |
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58 |
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"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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59 * <http://compomics.github.io/projects/peptide-shaker.html/> |
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60 * <http://compomics.github.io/projects/searchgui.html/> |
24 | 61 |
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62 GalaxyP Community |
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63 ----------------- |
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64 |
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65 Current governing community policies for GalaxyP_ and other information can be found at: |
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66 |
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67 <https://github.com/galaxyproteomics> |
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68 |
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69 .. _GalaxyP: https://github.com/galaxyproteomics/ |
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70 |
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71 |
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72 License |
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73 ------- |
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74 |
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75 Copyright (c) 2014 Regents of the University of Minnesota and Authors listed below. |
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76 |
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77 To the extent possible under law, the author(s) have dedicated all copyright and related and neighboring rights to this software to the public domain worldwide. This software is distributed without any warranty. |
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78 |
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79 You should have received a copy of the CC0 Public Domain Dedication along with this software. If not, see <https://creativecommons.org/publicdomain/zero/1.0/>. |
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80 |
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81 You can copy, modify, distribute and perform the work, even for commercial purposes, all without asking permission. |
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82 |
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83 |
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84 Contributing |
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85 ------------ |
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86 |
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87 Contributions to this repository are reviewed through pull requests. If you would like your work acknowledged, please also add yourself to the Authors section. If your pull request is accepted, you will also be acknowledged in <https://github.com/galaxyproteomics/tools-galaxyp/> |
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88 |
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89 |
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90 Authors |
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91 ------- |
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92 |
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93 Authors and contributors: |
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94 |
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95 * Bjoern Gruening <bjoern.gruening@gmail.com> |
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96 * Ira Cooke |
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97 * Cody Wang |
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98 * Fred Sadler |
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99 * John Chilton <jmchilton@gmail.com> |
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100 * Gerben Menschaert |
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101 * Elvis Ndah |
20
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102 * Minnesota Supercomputing Institute, Univeristy of Minnesota |
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"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/master/tools/peptideshaker commit f27e376ff604cd8faf57c63f25ddcfd61ca9db6a"
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103 * Carlos Horro |