annotate micropita.xml @ 0:2f4f6f08c8c4 draft

Uploaded
author george-weingart
date Tue, 13 May 2014 21:58:57 -0400
parents
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
0
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
1 <tool id="micropita" name="Run" version="1.0.1">
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
2 <code file="micropita_format_input_selector.py"/>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
3 <description>micropita</description>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
4 <command interpreter="python">micropita_prepare.py
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
5 --lastmeta $cls_x
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
6 -m $cond.method_sel
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
7 -n $selected_samples
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
8 --input $inp_data
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
9 --output $out_file1
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
10 --stratify_value $cls_s
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
11
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
12 #if $cond.method_sel == "features":
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
13 --feature_method $cond.feature_method
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
14 --targets $cond.cls_f
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
15 #end if
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
16 #if $cond.method_sel == "distinct" or $cond.method_sel == "discriminant" :
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
17 --label_value $cond.cls_L
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
18 #end if
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
19
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
20 </command>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
21 <inputs>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
22 <param format="micropita" name="inp_data" type="data" label="Input file"/>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
23
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
24 <param name="cls_x" type="select" label="Last metadata row (Use 'Label' for demo data)" multiple="False" size ="70" dynamic_options="get_cols(inp_data,'0')"/>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
25
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
26
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
27 <param name="cond" type="data_column" data_ref="inp_data" accept_default="true" />
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
28 <conditional name="cond" type="data_column" data_ref="inp_data" accept_default="true">
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
29 <param name="method_sel" type="select" data_ref="inp_data" label="Select method">
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
30 <option value="representative" selected="True">Representative</option>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
31 <option value="diverse">Diverse</option>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
32 <option value="extreme" >Extreme</option>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
33 <option value="features" >Features</option>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
34 <option value="distinct" >Distinct (Supervised)</option>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
35 <option value="discriminant" >Discriminant (Supervised)</option>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
36 </param>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
37 <when value="representative">
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
38 </when>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
39 <when value="diverse">
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
40 </when>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
41 <when value="extreme">
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
42 </when>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
43 <when value="features">
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
44 <param name="cls_f" type="select" label="Targeted feature(s)" multiple="True" size ="70" dynamic_options="get_cols_features(inp_data,'0',cls_x)"/>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
45 <param name="feature_method" type="select" format="text">
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
46 <label>Selection type</label>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
47 <option value="rank">Rank</option>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
48 <option value="abundance">Abundance</option>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
49 </param>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
50 </when>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
51 <when value="distinct">
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
52 <param name="cls_L" type="select" label="Label (Use 'Group' for demo data)" multiple="False" size ="70" dynamic_options="get_cols_add_line(inp_data,'0',cls_x)"/>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
53 </when>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
54
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
55 <when value="discriminant">
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
56 <param name="cls_L" type="select" label="Label (Use 'Group' for demo data)" multiple="False" size ="70" dynamic_options="get_cols_add_line(inp_data,'0',cls_x)"/>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
57 </when>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
58
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
59 </conditional>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
60
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
61
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
62 <param name="cls_s" type="select" label="Stratify by (optional) (Use 'StratifyLabel' for demo data)" multiple="False" size ="70" dynamic_options="get_cols_add_line(inp_data,'0',cls_x)"/>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
63
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
64 <param name="selected_samples" type="integer" size="4" value="10" label="Number of samples to select"/>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
65
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
66
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
67
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
68 </inputs>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
69
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
70 <outputs>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
71 <data format="text" name="out_file1" />
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
72 </outputs>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
73 <requirements>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
74 <requirement type="set_environment">micropita_SCRIPT_PATH</requirement>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
75 </requirements>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
76 <help>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
77
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
78
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
79 microbiome: Picking Interesting Taxonomic Abundance
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
80 ---------------------------------------------------
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
81
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
82
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
83
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
84 microPITA is a computational tool enabling sample selection in tiered studies. Using tiered-study designs can more efficiently allocate resources, reducing study costs, and maximizing the use of samples. From a survey study, selection of samples can be performed to target various microbial communities including:
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
85
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
86 1. Samples with the most diverse community (maximum diversity);
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
87 2. Samples dominated by specific microbes (targeted feature);
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
88 3. Samples with microbial communities representative of the survey (representative dissimilarity);
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
89 4. Samples with the most extreme microbial communities in the survey (most dissimilar);
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
90 5. Given a phenotype (like disease state), samples at the border of phenotypes (discriminant) or samples typical of each phenotype (distinct).
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
91
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
92 Additionally, methods can leverage clinical metadata by stratifying samples into groups in which samples are subsequently selected. This enables the use of microPITA in cohort studies.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
93
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
94
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
95 .. image:: https://bytebucket.org/biobakery/galaxy_micropita/wiki/HMPStool10PCoA.png
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
96 :height: 500
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
97 :width: 600
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
98
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
99 MicroPITA unsupervised method selection in the HMP 16S Gut Microbiome. Selection of 10 samples using targeted feature targeting *Bacteroides* (blue), maximum diversity (orange), representative dissimilarity (purple), and most dissimilar (pink) using Principle Covariance Analysis (PCoA) for ordination. Targeted feature selects samples dominated by *Bacteroides* (upper left) while maximum diversity select more diverse samples away from *Bacteroides* dominant samples. Representative selection selects samples covering the range of samples in the PCoA plot focusing on the higher density central region while maximum dissimilarity selects samples at the periphery of the plot.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
100
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
101
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
102 Intructions to run:
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
103 -------------------
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
104
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
105 Before running microPita, you must upload your data using Glaxay's **Get Data - Upload File**
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
106 Please make sure that you choose **File Format Micropita**
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
107 An example can be found at https://bytebucket.org/biobakery/micropita/wiki/micropita_sample_PCL.txt
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
108
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
109 Required inputs
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
110 ---------------
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
111
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
112 microPITA requires an input pcl file of metadata and microbial community measurements. Although some defaults can be changed, microPITA expects a PCL file as an input file. A PCL file is a text delimited file similar to an excel spread sheet with the following characteristics.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
113
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
114 1. **Rows** represent metadata and features (bugs), **columns** represent samples.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
115 2. The **first row** by default should be the sample ids.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
116 3. Metadata rows should be next.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
117 4. Lastly, rows containing features (bugs) measurements (like abundance) should be after metadata rows.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
118 5. The **first column** should contain the ID describing the column. For metadata this may be, for example, "Age" for a row containing the age of the patients donating the samples. For measurements, this should be the feature name (bug name).
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
119 6. The file is expected to be TAB delimited.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
120 7. If a consensus lineage or hierarchy of taxonomy is contained in the feature name, the default delimiter between clades is the pipe ("|").
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
121
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
122 **Note** MAC users, please save file as windows formatted text.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
123
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
124 .. image:: https://bytebucket.org/biobakery/galaxy_micropita/wiki/pcl_diagram.png
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
125 :height: 500
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
126 :width: 600
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
127
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
128 Outputs
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
129 -------
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
130
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
131 The Run MicroPITA module will create one output text file. The output will consist of one line starting with a key word for the selection method and then followed by selected samples delimited by tabs. An example of 6 samples selected by the representative:
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
132
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
133 representative sample_1 sample_2 sample_3 sample_4 sample_5 sample_6
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
134
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
135
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
136
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
137
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
138 Run microPITA
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
139 -------------
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
140
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
141 A brief description of the Run micropita module.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
142
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
143 **Input file:**
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
144 This should be populated by the Load microPITA module.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
145
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
146 **Last metadata row:**
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
147 The row on the input pcl file that is the last metadata. All microbial measurements should follow this row.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
148
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
149 **Select method:**
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
150 Select which method to use for sample selection. Selection methods include:
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
151
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
152 1. Representative. Samples with microbial communities representative of the survey (representative dissimilarity);
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
153 2. Diverse. Samples with the most diverse community (maximum diversity);
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
154 3. Extreme. Samples with the most extreme microbial communities in the survey (most dissimilar);
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
155 4. Features. Samples dominated by specific microbes (targeted feature);
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
156 5. Distinct. Given a phenotype (like disease state), samples typical of each phenotype (Distinct).
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
157 6. Discriminant. Given a phenotype (like disease state), samples at the border of phenotypes (Discriminant).
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
158
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
159 **Targeted feature(s):** (visible with Features method selection only)
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
160 Select 1 or more features to target in sample selection.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
161
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
162 **Selection type:** (visible with Features method selection only)
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
163 Rank or Abundance.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
164
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
165 1. Rank indicates selecting samples that have the highest rank of the Targeted features(s), this tends to select sample in which these feature dominant the sample.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
166 2. Abundance indicates selecting samples that have the highest average abundance of the Targeted features(s), this selects samples where features are most abundant but not necessarily dominant in the community.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
167
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
168 **Label:** (visible with supervised method selection only)
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
169 The row which contains the label used to classify the samples from supervised methods.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
170
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
171 **Stratify by (optional):**
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
172 The row which contains the groupings the samples will first be placed in before running the selection method on each group. If no grouping is selected, selection methods will be performed on the data set as a whole.
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
173
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
174 **Number of samples to select:**
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
175 The number of samples to select. If samples are stratified, this is per stratification (or group). If supervised methods are used, this is the number of samples selected per classification group (as defined by the label).
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
176
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
177 For more information please visit http://huttenhower.sph.harvard.edu/micropita
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
178
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
179
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
180 Acknowledgments
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
181 ---------------
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
182 Special thanks to Eric Franzosa for developing the above PCL figure!
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
183
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
184 Citation and Contacts
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
185 ---------------------
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
186
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
187 For more information please visit http://huttenhower.sph.harvard.edu/micropita
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
188 When using MicroPITA please cite:
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
189 Tickle T, Segata N, Waldron L, Weingart G, Huttenhower C. Two-stage microbial community experimental design. (Under review)
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
190
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
191 Please feel free to contact us at ttickle@hsph.harvard.edu for any questions or comments!
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
192
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
193
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
194 </help>
2f4f6f08c8c4 Uploaded
george-weingart
parents:
diff changeset
195 </tool>