Mercurial > repos > ieguinoa > data_manager_salmon_index_builder
view data_manager/salmon_index_builder.py @ 9:9004217cddf4 draft default tip
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author | ieguinoa |
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date | Tue, 23 Jul 2019 12:18:12 -0400 |
parents | cbe62bd6e149 |
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#!/usr/bin/env python # Based heavily on the kallisto data manager wrapper script by iuc from __future__ import print_function import argparse import os import subprocess import sys from json import dumps, loads DEFAULT_DATA_TABLE_NAME = "salmon_indexes_versioned" def get_id_name( params, dbkey, fasta_description=None): # TODO: ensure sequence_id is unique and does not already appear in location file sequence_id = params['param_dict']['sequence_id'] if not sequence_id: sequence_id = dbkey sequence_name = params['param_dict']['sequence_name'] if not sequence_name: sequence_name = fasta_description if not sequence_name: sequence_name = dbkey return sequence_id, sequence_name def build_salmon_index( data_manager_dict, options, params, sequence_id, sequence_name ): data_table_name = options.data_table_name or DEFAULT_DATA_TABLE_NAME target_directory = params[ 'output_data' ][0]['extra_files_path'] if not os.path.exists( target_directory ): os.mkdir( target_directory ) args = [ 'salmon', 'index' ] if options.kmer_size != '': args.append('-k') args.append(options.kmer_size) args.extend( [ '-t', options.fasta_filename, '-i', target_directory ] ) proc = subprocess.Popen( args=args, shell=False) return_code = proc.wait() if return_code: print("Error building index.", file=sys.stderr) sys.exit( return_code ) data_table_entry = dict( value=sequence_id, dbkey=options.fasta_dbkey, name=sequence_name, path=sequence_id, version=options.index_version ) _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ) def _add_data_table_entry( data_manager_dict, data_table_name, data_table_entry ): data_manager_dict['data_tables'] = data_manager_dict.get( 'data_tables', {} ) data_manager_dict['data_tables'][ data_table_name ] = data_manager_dict['data_tables'].get( data_table_name, [] ) data_manager_dict['data_tables'][ data_table_name ].append( data_table_entry ) return data_manager_dict def main(): # Parse Command Line parser = argparse.ArgumentParser() parser.add_argument( '--output', dest='output', action='store', type=str, default=None ) parser.add_argument( '--fasta_filename', dest='fasta_filename', action='store', type=str, default=None ) parser.add_argument( '--fasta_dbkey', dest='fasta_dbkey', action='store', type=str, default=None ) parser.add_argument( '--fasta_description', dest='fasta_description', action='store', type=str, default=None ) parser.add_argument( '--data_table_name', dest='data_table_name', action='store', type=str, default='salmon_indexes' ) parser.add_argument( '-v','--index_version', dest='index_version', action='store', type=str, help='Use IndexVersion attribute from header.json' ) parser.add_argument( '-k', '--kmer_size', dest='kmer_size', action='store', type=str, help='kmer_size' ) options = parser.parse_args() filename = options.output params = loads( open( filename ).read() ) data_manager_dict = {} if options.fasta_dbkey in [ None, '', '?' ]: raise Exception( '"%s" is not a valid dbkey. You must specify a valid dbkey.' % ( options.fasta_dbkey ) ) sequence_id, sequence_name = get_id_name( params, dbkey=options.fasta_dbkey, fasta_description=options.fasta_description ) # build the index build_salmon_index( data_manager_dict, options, params, sequence_id, sequence_name ) # save info to json file open( filename, 'w' ).write( dumps( data_manager_dict ) ) if __name__ == "__main__": main()