Mercurial > repos > ieguinoa > ena_webin_cli

changeset 2:1ecd8ce07db4 draft

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Uploaded
author ieguinoa
date Fri, 04 Feb 2022 15:52:45 +0000
parents f24eb2f2cb0c
children 7d751b5943b0
files ena_consensus_submit.xml test-data/phiX2.fasta
diffstat 2 files changed, 175 insertions(+), 47 deletions(-) [+]
line wrap: on
line diff
--- a/ena_consensus_submit.xml	Tue May 18 21:00:09 2021 +0000
+++ b/ena_consensus_submit.xml	Fri Feb 04 15:52:45 2022 +0000
@@ -1,6 +1,6 @@
-<tool id="ena_consensus_submit" name="Submit consensus sequence to ENA" version="0.1.0" python_template_version="3.5">
+<tool id="ena_consensus_submit" name="Submit consensus sequence to ENA" version="@VERSION@" python_template_version="3.5">
     <macros>
-        <token name="@VERSION@">3.7.0</token>
+        <token name="@VERSION@">4.3.0</token>
     </macros>
     <requirements>
         <requirement type="package" version="@VERSION@">ena-webin-cli</requirement>
@@ -8,16 +8,22 @@
         <requirement type="package" version="5.3">pyyaml</requirement>
     </requirements>
     <command detect_errors="exit_code"><![CDATA[
+#if $test_submit == "True":
+    #set $credentials='./test_fake_path';
+    touch $credentials;
+    echo "username:test_user" >> $credentials;
+    echo "password:test_password" >> $credentials;
+#end if
 
-webin_id=`grep 'username' $credentials | cut -f2,2`;
+webin_id=`grep 'username' $credentials | cut -d':' -f2,2`;
 if [ "\$webin_id" = "" ]; then
   ## No credentials in user defined preferences    
   ## Fallback to global defined credentials (if exist)   
   #import os
   #if os.path.isfile(os.environ.get('GALAXY_ENA_SECRETS', '')):
       credentials_path=\${GALAXY_ENA_SECRETS};     
-      webin_id=`grep 'username' \$GALAXY_ENA_SECRETS | cut -d' ' -f2,2`;
-      password=`grep 'password' \$GALAXY_ENA_SECRETS | cut -d' ' -f2,2`;
+      webin_id=`grep 'username' \$GALAXY_ENA_SECRETS | cut -d':' -f2,2` | sed -e 's/^[[:space:]]*//'`;
+      password=`grep 'password' \$GALAXY_ENA_SECRETS | cut -d':' -f2,2` | sed -e 's/^[[:space:]]*//'`;
       if [ "\$webin_id" = "" ]; then
           echo "No global credentials defined. Check your GALAXY_ENA_SECRETS file or set your credentials via: User -> Preferences -> Manage Information";
           exit 1;
@@ -27,23 +33,22 @@
       exit 1;
   #end if
 else
-  password=`grep 'password' $credentials | cut -f2,2`;
+  password=`grep 'password' $credentials | cut -d':' -f2,2`;
 fi;
 
-## if it is still running at this point then the webin_id and password are set
 
-## create a manifaste base file with the parameters that are identical for all sequences that will be submited (if submitting a multifasta)
+## create a manifest base file with the parameters that are identical for all sequences that will be submited (if submitting a multifasta)
 #set $manifest_base = 'manifest_base.tab'
 mkdir manifests;
 mkdir fasta;
 
-echo -e "ASSEMBLY_TYPE\t$assembly_type" >> $manifest_base;
-echo -e "COVERAGE\t$coverage" >> $manifest_base;
-echo -e "PROGRAM\t$assembly_program" >> $manifest_base;
+echo -e 'ASSEMBLY_TYPE\t$assembly_type' > $manifest_base;
+echo -e 'COVERAGE\t$coverage' >> $manifest_base;
+echo -e 'PROGRAM\t$assembly_program' >> $manifest_base;
 #if $min_gap_length:
-    echo -e "MINGAPLENGTH\t$min_gap_length" >> $manifest_base;
+    echo -e 'MINGAPLENGTH\t$min_gap_length' >> $manifest_base;
 #end if
-echo -e "MOLECULETYPE\t$molecule_type" >> $manifest_base;
+echo -e 'MOLECULETYPE\t$molecule_type' >> $manifest_base;
         
 #if $metadata_file_or_form.metadata_format == "file":
     ## process the input tables, this creates an intermediate file with information
@@ -51,17 +56,18 @@
     center_name=`grep 'center_name' $metadata_file_or_form.ena_receipt | cut -f2,2 | tr -d '\n'`;
 #else:
     #set $generated_manifest='./manifests/generated_manifest.txt'
-    cp $manifest_base $generated_manifest
-    $study_id = $metadata_file_or_form.study_accession
-    $sample_id = $metadata_file_or_form.sample_accession
-    echo "STUDY\t$study_id" > $generated_manifest;
-    echo "SAMPLE\t$sample_id" >> $generated_manifest;
-    center_name=$metadata_file_or_form.center_name;
-    echo "ASSEMBLY_NAME\t$metadata_file_or_form.assembly_name" >> $generated_manifest;
-    echo "PLATFORM\t$platform_name" >> $generated_manifest;
+    cp $manifest_base $generated_manifest;
+    #set $study_id = $metadata_file_or_form.study_accession
+    #set $sample_id = $metadata_file_or_form.sample_accession
+    echo -e 'STUDY\t$study_id' >> $generated_manifest;
+    echo -e 'SAMPLE\t$sample_id' >> $generated_manifest;
+    center_name='$metadata_file_or_form.center_name';
+    echo -e 'NAME\t$metadata_file_or_form.assembly_name' >> $generated_manifest;
+    echo -e 'PLATFORM\t$metadata_file_or_form.sequencing_platform' >> $generated_manifest;
 #end if
-        
 
+#set $outputs_dir = 'outputs'
+mkdir $outputs_dir;
 #if $metadata_file_or_form.metadata_format == "file":
     ## iterate over the list of manifest - fasta generated by the process_input
     ## in case of errors, this list is empty
@@ -69,27 +75,22 @@
         manifest=`echo \$line | cut -d' ' -f1,1`;
         ena-webin-cli
         -context genome
-        -userName \$webin_id
-        -password \$password
-        -centerName \$center_name 
+        -userName "'\$webin_id'"
+        -password "'\$password'"
+        -centerName "'\$center_name'" 
         -manifest \$manifest
-        -inputDir "./fasta"
+        -inputDir './fasta'
         #if $dry_run == "true":
             -validate
         #end if
-        ;
+        -outputDir $outputs_dir
+        >> $webin_cli_log ;
     done < submit_list.tab
 
 #else:
     gzip -c $genome_fasta > consensus.fasta.gz;
-    echo -e "FASTA\tconsensus.fasta.gz" >> $generated_manifest;
+    echo -e 'FASTA\tconsensus.fasta.gz' >> $generated_manifest;
     ena-webin-cli
-    -context genome 
-    -userName \$webin_id
-    -password \$password
-    -centerName '\$center_name' 
-    -manifest $generated_manifest
-    -inputDir "./"
     #if $submit_test == "true":
         -test
     #end if
@@ -98,21 +99,33 @@
     #else:
         -submit
     #end if
+    -context genome 
+    -manifest $generated_manifest
+    -password "'\$password'"
+    -userName "'\$webin_id'"
+    -inputDir "./"
+    -centerName "'\$center_name'"
+    -outputDir $outputs_dir
+    >> $webin_cli_log ;
+    ##cp ./genome/$metadata_file_or_form.assembly_name/validate/webin-cli.report $validate_output
 #end if
+tar -cf $webin_cli_outputs $outputs_dir;
 ]]></command>
     <configfiles>
         <configfile name="credentials"><![CDATA[
 #set $webin_id = $__user__.extra_preferences.get('ena_webin_account|webin_id', "").strip()
 #set $password = $__user__.extra_preferences.get('ena_webin_account|password', "").strip()
 #if $webin_id != "":
-    username\t"$webin_id"
-    password\t"$password"
+    username:$webin_id
+    password:$password
 #end if
+
         ]]></configfile>
     </configfiles>
 <inputs>
-    <param name="submit_test" type="boolean" default="False" label="Submit to test server" help="Suggested to test metadata format" />
-    <param name="dry_run" type="boolean" default="False" label="Validate files and metadata but do not submit" help="Generate input files and run Webin-CLI with -validate option."/>
+    <param name="test_submit" type="hidden" value="False" />
+    <param name="submit_test" type="boolean" truevalue="true" falsevalue="false" label="Submit to test server" help="use Webin test service instead of the production service. Please note that the Webin upload area is shared between test and production services, and that test submission files will not be archived." />
+    <param name="dry_run" type="boolean" truevalue="true" falsevalue="false" label="Validate files and metadata but do not submit" help="Generate input files and run Webin-CLI with -validate option. If 'No' is selected then it will validate and submit (-submit flag)"/>
     <param name="genome_fasta" type="data" label="Select the consensus sequence assembly file" format="fasta"/>
     <param name="assembly_type" type="select" label="Assembly type">
         <option value="clone">Clone</option>
@@ -127,8 +140,8 @@
     <param name="coverage" type="float" optional="False" value="10000" label="Coverage"/>
     <conditional name="metadata_file_or_form">
         <param name="metadata_format" type="select" label="Select the method to load study and sample metadata">
-            <option value="file" selected="True">I used Galaxy ENA upload tool for raw data submission, parse my submission receipt</option>
-            <option value="form">Fill in required metadata for linking</option>
+            <option value="form" selected="True">Fill in required submission metadata</option>
+            <option value="file">(Beta) I used the Galaxy ENA upload tool to submit the raw data: parse the received submission receipt</option>
         </param>
         <when value="file">
             <param type="data" format="txt" name="ena_receipt" label="Submission receipt obtained from ENA upload tool"/>
@@ -146,15 +159,40 @@
     </inputs>
     <outputs>
         <collection name="generated_manifests" type="list" label="Generated manifests">
-            <!--<discover_datasets pattern="__name_and_ext__" directory="manifests" />-->
-            <discover_datasets pattern="(?P&lt;designation&gt;.+)\.manifest\.txt$" ext="txt" directory="manifests" />
+            <discover_datasets pattern="(?P&lt;designation&gt;.+)\.txt" ext="txt" directory="manifests/"/>
         </collection>
-        <collection name="manifests_reports" type="list" label="Manifests reports">
-            <!--[><discover_datasets pattern="__name_and_ext__" directory="manifests" /><]-->
-            <discover_datasets pattern="(?P&lt;designation&gt;.+)\.manifest\.txt\.report$" ext="txt" directory="manifests" />
-        </collection>
-        <data name="webin_cli_report" label="ENA submission receipt" format="txt" from_work_dir="manifests/webin-cli.report"/>
+        <data name="webin_cli_log" label="ENA submission log" format="txt"/>
+        <data name="webin_cli_outputs" label="Webin cli outputs" format="tar"/>
+        
     </outputs>
+    <tests>
+        <test>
+            <param name="submit_test" value="true" />
+            <param name="dry_run" value="true" />
+            <param name="test_submit" value="True" />
+            <param name="genome_fasta" value="phiX2.fasta"/>
+            <param name="assembly_type" value="isolate"/>
+            <param name="assembly_program" value="Test assembly program"/>
+            <param name="molecule_type" value="viral cRNA"/>
+            <param name="coverage" value="10000"/>
+            <conditional name="metadata_file_or_form">
+                <param name="metadata_format" value="form"/>
+                <param name="assembly_name" value="Test assembly name"/>
+                <param name="study_accession" value="PRJEB49173"/>
+                <param name="sample_accession" value="SAMEA11953908"/>
+                <param name="sequencing_platform" value="Nanopore 0011"/>
+                <param name="description" value="Test Description"/>
+                <param name="center_name" value="Test center name"/>
+            </conditional>
+            <param name="min_gap_length" value="30"/>
+            <output name="webin_cli_log">
+                <assert_contents>
+                    <has_n_lines n="4"/>
+                    <has_text_matching expression="ERROR: Invalid submission account user name or password."/>
+                </assert_contents>
+            </output>
+        </test>
+    </tests>
     <help><![CDATA[
         TODO: Fill in help.
     ]]></help>
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/phiX2.fasta	Fri Feb 04 15:52:45 2022 +0000
@@ -0,0 +1,90 @@
+>phiX174
+GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTT
+GATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAA
+ATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTG
+TCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTA
+GATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATC
+TGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTT
+TCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTT
+CGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCT
+TGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCG
+TCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTAC
+GGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTA
+CGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAAGGAG
+TGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACT
+AAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC
+CCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCA
+TCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGAC
+TCCTTCGAGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGCTATTGACTCTA
+CTGTAGACATTTTTACTTTTTATGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAA
+GGATGGTGTTAATGCCACTCCTCTCCCGACTGTTAACACTACTGGTTATATTGACCATGCCGCTTTTCTT
+GGCACGATTAACCCTGATACCAATAAAATCCCTAAGCATTTGTTTCAGGGTTATTTGAATATCTATAACA
+ACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGC
+TCGTTATGGTTTCCGTTGCTGCCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTT
+TCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC
+ATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCGTGATGTTATTTCTTCATTTGGAGGTAAAAC
+CTCTTATGACGCTGACAACCGTCCTTTACTTGTCATGCGCTCTAATCTCTGGGCATCTGGCTATGATGTT
+GATGGAACTGACCAAACGTCGTTAGGCCAGTTTTCTGGTCGTGTTCAACAGACCTATAAACATTCTGTGC
+CGCGTTTCTTTGTTCCTGAGCATGGCACTATGTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGAC
+TAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTG
+TATGGCAACTTGCCGCCGCGTGAAATTTCTATGAAGGATGTTTTCCGTTCTGGTGATTCGTCTAAGAAGT
+TTAAGATTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATCACCTTCTTGA
+AGGCTTCCCATTCATTCAGGAACCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGAT
+TATGACCAGTGTTTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGTGACCGTTT
+ATCGCAATCTGCCGACCACTCGCGATTCAATCATGACTTCGTGATAAAAGATTGAGTGTGAGGTTATAAC
+GCCGAAGCGGTAAAAATTTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGC
+TTAGGAGTTTAATCATGTTTCAGACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGT
+TCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTA
+TATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTG
+TCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGATATTGCTTTTGATGCCGACCCTAAATTTTTTGC
+CTGTTTGGTTCGCTTTGAGTCTTCTTCGGTTCCGACTACCCTCCCGACTGCCTATGATGTTTATCCTTTG
+AATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCCTTCCCCGTACGC
+CGGGCAATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGT
+TTCGCTGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGTTTGGTG
+CTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAAA
+AGCCGCCTCCGGTGGCATTCAAGGTGATGTGCTTGCTACCGATAACAATACTGTAGGCATGGGTGATGCT
+GGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAGGCCGCCCCTAGTTTTGTTTCTG
+GTGCTATGGCTAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGA
+TAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTAT
+CTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGG
+TTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGA
+GATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGCCAGAATACGAAAGAC
+CAGGTATATGCACAAAATGAGATGCTTGCTTATCAACAGAAGGAGTCTACTGCTCGCGTTGCGTCTATTA
+TGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTCCGAGATTATGCGCCAAATGCTTACTCAAGCTCA
+AACGGCTGGTCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTGCTGAGGTTGAC
+TTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAGGATATTT
+CTAATGTCGTCACTGATGCTGCTTCTGGTGTGGTTGATATTTTTCATGGTATTGATAAAGCTGTTGCCGA
+TACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAACCG
+TCAGGATTGACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTTTATGGTTCGTT
+CTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTAT
+TGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCTTGGCTTCCATAAGCAGATGGATAACCGC
+ATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATG
+TTGACGGCCATAAGGCTGCTTCTGACGTTCGTGATGAGTTTGTATCTGTTACTGAGAAGTTAATGGATGA
+ATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGG
+GACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCC
+CTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATT
+GCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTACTATTCAGCGTTTGATGAATGCAATGCGACAG
+GCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGAGGCGTTTT
+ATGATAATCCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGCTGCCGAGGGTCG
+CAAGGCTAATGATTCACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGC
+CGTCTTCATTTCCATGCGGTGCATTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTC
+GTCGGGTACGCAATCGCCGCCAGTTAAATAGCTTGCAAAATACGTGGCCTTATGGTTACAGTATGCCCAT
+CGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGGTGAG
+CCGCTTAAAGCTACCAGTTATATGGCTGTTGGTTTCTATGTGGCTAAATACGTTAACAAAAAGTCAGATA
+TGGACCTTGCTGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACT
+TCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTG
+TCCACGGAGTGCTTAATCCAACTTACCAAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGC
+AGAACGCAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTTTTGGCGGCGCAACC
+TGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATTGGCGTATCCAACCTGCA
+>phi2174
+GAGTTTTATCGCTTCCATGACGCAGAAGTTAACACTTTCGGATATTTCTGATGAGTCGAAAAATTATCTT
+GATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGACTGCTGGCGGAAAATGAGAAA
+ATTCGACCTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACGATTCTG
+TCAAAAACTGACGCGTTGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGGTTTA
+GATATGAGTCACATTTTGTTCATGGTAGAGATTCTCTTGTTGACATTTTAAAAGAGCGTGGATTACTATC
+TGAGTCCGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTT
+TCCAGACCGCTTTGGCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGATGATTT
+CGATTTTCTGACGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGCGTTGAGGCT
+TGCGTTTATGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCG
+TCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTAC
+GGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTA