annotate 2d_feature_extraction.py @ 5:2436a8807ad1 draft

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date Thu, 04 Apr 2024 15:23:23 +0000
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1 import argparse
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3 import numpy as np
96909b9d1df1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tools/2d_feature_extraction/ commit c3f4b766f03770f094fda6bda0a5882c0ebd4581
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4 import pandas as pd
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5 import skimage.feature
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6 import skimage.io
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7 import skimage.measure
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8 import skimage.morphology
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9 import skimage.segmentation
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12 if __name__ == '__main__':
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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14 parser = argparse.ArgumentParser(description='Extract image features')
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16 # TODO create factory for boilerplate code
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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17 features = parser.add_argument_group('compute features')
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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18 features.add_argument('--all', dest='all_features', action='store_true')
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19 features.add_argument('--label', dest='add_label', action='store_true')
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20 features.add_argument('--patches', dest='add_roi_patches', action='store_true')
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21 features.add_argument('--max_intensity', dest='max_intensity', action='store_true')
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22 features.add_argument('--mean_intensity', dest='mean_intensity', action='store_true')
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23 features.add_argument('--min_intensity', dest='min_intensity', action='store_true')
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24 features.add_argument('--moments_hu', dest='moments_hu', action='store_true')
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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25 features.add_argument('--centroid', dest='centroid', action='store_true')
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26 features.add_argument('--bbox', dest='bbox', action='store_true')
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27 features.add_argument('--area', dest='area', action='store_true')
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28 features.add_argument('--filled_area', dest='filled_area', action='store_true')
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29 features.add_argument('--convex_area', dest='convex_area', action='store_true')
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30 features.add_argument('--perimeter', dest='perimeter', action='store_true')
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31 features.add_argument('--extent', dest='extent', action='store_true')
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32 features.add_argument('--eccentricity', dest='eccentricity', action='store_true')
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33 features.add_argument('--equivalent_diameter', dest='equivalent_diameter', action='store_true')
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34 features.add_argument('--euler_number', dest='euler_number', action='store_true')
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35 features.add_argument('--inertia_tensor_eigvals', dest='inertia_tensor_eigvals', action='store_true')
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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36 features.add_argument('--major_axis_length', dest='major_axis_length', action='store_true')
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37 features.add_argument('--minor_axis_length', dest='minor_axis_length', action='store_true')
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38 features.add_argument('--orientation', dest='orientation', action='store_true')
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39 features.add_argument('--solidity', dest='solidity', action='store_true')
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40 features.add_argument('--moments', dest='moments', action='store_true')
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41 features.add_argument('--convexity', dest='convexity', action='store_true')
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43 parser.add_argument('--label_file_binary', dest='label_file_binary', action='store_true')
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45 parser.add_argument('--raw', dest='raw_file', type=argparse.FileType('r'),
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46 help='Original input file', required=False)
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47 parser.add_argument('label_file', type=argparse.FileType('r'),
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48 help='Label input file')
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49 parser.add_argument('output_file', type=argparse.FileType('w'),
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50 help='Tabular output file')
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51 args = parser.parse_args()
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53 label_file_binary = args.label_file_binary
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54 label_file = args.label_file.name
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55 out_file = args.output_file.name
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56 add_patch = args.add_roi_patches
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58 raw_image = None
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59 if args.raw_file is not None:
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60 raw_image = skimage.io.imread(args.raw_file.name)
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62 raw_label_image = skimage.io.imread(label_file)
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64 df = pd.DataFrame()
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65 if label_file_binary:
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66 raw_label_image = skimage.measure.label(raw_label_image)
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67 regions = skimage.measure.regionprops(raw_label_image, intensity_image=raw_image)
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69 df['it'] = np.arange(len(regions))
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71 if add_patch:
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72 df['image'] = df['it'].map(lambda ait: regions[ait].image.astype(np.float).tolist())
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73 df['intensity_image'] = df['it'].map(lambda ait: regions[ait].intensity_image.astype(np.float).tolist())
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75 # TODO no matrix features, but split in own rows?
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76 if args.add_label or args.all_features:
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77 df['label'] = df['it'].map(lambda ait: regions[ait].label)
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79 if raw_image is not None:
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80 if args.max_intensity or args.all_features:
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81 df['max_intensity'] = df['it'].map(lambda ait: regions[ait].max_intensity)
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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82 if args.mean_intensity or args.all_features:
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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83 df['mean_intensity'] = df['it'].map(lambda ait: regions[ait].mean_intensity)
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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84 if args.min_intensity or args.all_features:
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85 df['min_intensity'] = df['it'].map(lambda ait: regions[ait].min_intensity)
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86 if args.moments_hu or args.all_features:
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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87 df['moments_hu'] = df['it'].map(lambda ait: regions[ait].moments_hu)
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88
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89 if args.centroid or args.all_features:
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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90 df['centroid'] = df['it'].map(lambda ait: regions[ait].centroid)
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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91 if args.bbox or args.all_features:
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92 df['bbox'] = df['it'].map(lambda ait: regions[ait].bbox)
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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93 if args.area or args.all_features:
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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94 df['area'] = df['it'].map(lambda ait: regions[ait].area)
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95 if args.filled_area or args.all_features:
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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96 df['filled_area'] = df['it'].map(lambda ait: regions[ait].filled_area)
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97 if args.convex_area or args.all_features:
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98 df['convex_area'] = df['it'].map(lambda ait: regions[ait].convex_area)
2436a8807ad1 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/2d_feature_extraction/ commit c045f067a57e8308308cf6329060c7ccd3fc372f
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99 if args.perimeter or args.all_features:
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100 df['perimeter'] = df['it'].map(lambda ait: regions[ait].perimeter)
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101 if args.extent or args.all_features:
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102 df['extent'] = df['it'].map(lambda ait: regions[ait].extent)
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103 if args.eccentricity or args.all_features:
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104 df['eccentricity'] = df['it'].map(lambda ait: regions[ait].eccentricity)
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105 if args.equivalent_diameter or args.all_features:
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106 df['equivalent_diameter'] = df['it'].map(lambda ait: regions[ait].equivalent_diameter)
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107 if args.euler_number or args.all_features:
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108 df['euler_number'] = df['it'].map(lambda ait: regions[ait].euler_number)
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109 if args.inertia_tensor_eigvals or args.all_features:
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110 df['inertia_tensor_eigvals'] = df['it'].map(lambda ait: regions[ait].inertia_tensor_eigvals)
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111 if args.major_axis_length or args.all_features:
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112 df['major_axis_length'] = df['it'].map(lambda ait: regions[ait].major_axis_length)
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113 if args.minor_axis_length or args.all_features:
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114 df['minor_axis_length'] = df['it'].map(lambda ait: regions[ait].minor_axis_length)
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115 if args.orientation or args.all_features:
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116 df['orientation'] = df['it'].map(lambda ait: regions[ait].orientation)
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117 if args.solidity or args.all_features:
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118 df['solidity'] = df['it'].map(lambda ait: regions[ait].solidity)
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119 if args.moments or args.all_features:
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120 df['moments'] = df['it'].map(lambda ait: regions[ait].moments)
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121 if args.convexity or args.all_features:
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122 perimeter = df['it'].map(lambda ait: regions[ait].perimeter)
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123 area = df['it'].map(lambda ait: regions[ait].area)
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124 df['convexity'] = area / (perimeter * perimeter)
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125
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126 del df['it']
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127 df.to_csv(out_file, sep='\t', line_terminator='\n', index=False)