annotate 2d_split_binaryimage_by_watershed.py @ 4:984358e43242 draft default tip

planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
author imgteam
date Tue, 14 Nov 2023 08:27:27 +0000
parents
children
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
4
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
1 import argparse
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
2 import sys
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
3
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
4 import skimage.io
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
5 import skimage.util
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
6 from scipy import ndimage as ndi
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
7 from skimage.feature import peak_local_max
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
8 from skimage.morphology import watershed
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
9
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
10
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
11 if __name__ == "__main__":
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
12 parser = argparse.ArgumentParser(description='Split binaryimage by watershed')
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
13 parser.add_argument('input_file', type=argparse.FileType('r'), default=sys.stdin, help='input file')
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
14 parser.add_argument('out_file', type=argparse.FileType('w'), default=sys.stdin, help='out file (TIFF)')
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
15 parser.add_argument('min_distance', type=int, default=100, help='Minimum distance to next object')
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
16 args = parser.parse_args()
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
17
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
18 img_in = skimage.io.imread(args.input_file.name)
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
19 distance = ndi.distance_transform_edt(img_in)
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
20 local_maxi = peak_local_max(distance,
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
21 indices=False,
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
22 min_distance=args.min_distance,
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
23 labels=img_in)
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
24 markers = ndi.label(local_maxi)[0]
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
25 res = watershed(-distance, markers, mask=img_in)
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
26
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
27 res = skimage.util.img_as_uint(res)
984358e43242 planemo upload for repository https://github.com/BMCV/galaxy-image-analysis/tree/master/tools/binary2labelimage/ commit 00199464fa2aa1928a49e2379edf199c3db91533
imgteam
parents:
diff changeset
28 skimage.io.imsave(args.out_file.name, res, plugin="tifffile")