Mercurial > repos > imgteam > imagej2_analyze_particles_binary
annotate imagej2_skeletonize3d_jython_script.py @ 1:1dd5396c734a draft
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
author | imgteam |
---|---|
date | Mon, 28 Sep 2020 16:59:30 +0000 |
parents | aeb9bb864b8c |
children | ae4ae9c5c56c |
rev | line source |
---|---|
0
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
1 import sys |
1
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
2 |
0
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
3 from ij import IJ |
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
4 |
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
5 # Fiji Jython interpreter implements Python 2.5 which does not |
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
6 # provide support for argparse. |
1
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
7 error_log = sys.argv[-5] |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
8 input_file = sys.argv[-4] |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
9 black_background = sys.argv[-3] == 'yes' |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
10 tmp_output_path = sys.argv[-2] |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
11 output_datatype = sys.argv[-1] |
0
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
12 |
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
13 # Open the input image file. |
1
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
14 input_image_plus = IJ.openImage(input_file) |
0
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
15 |
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
16 # Create a copy of the image. |
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
17 input_image_plus_copy = input_image_plus.duplicate() |
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
18 image_processor_copy = input_image_plus_copy.getProcessor() |
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
19 |
1
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
20 # Set binary options. |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
21 options = ['edm=Overwrite', 'iterations=1', 'count=1'] |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
22 if (black_background): |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
23 options.append('black') |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
24 options = " ".join(options) |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
25 IJ.run(input_image_plus_copy, "Options...", options) |
0
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
26 |
1
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
27 # Convert image to binary if necessary. |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
28 if not image_processor_copy.isBinary(): |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
29 # Convert the image to binary grayscale. |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
30 IJ.run(input_image_plus_copy, "Make Binary", "") |
0
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
31 |
1
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
32 # Run the command. |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
33 IJ.run(input_image_plus_copy, "Skeletonize (2D/3D)", "") |
0
aeb9bb864b8c
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
imgteam
parents:
diff
changeset
|
34 |
1
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
35 # Save the ImagePlus object as a new image. |
1dd5396c734a
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit 2afb24f3c81d625312186750a714d702363012b5"
imgteam
parents:
0
diff
changeset
|
36 IJ.saveAs(input_image_plus_copy, output_datatype, tmp_output_path) |