Mercurial > repos > imgteam > imagej2_bunwarpj_compose_raw
annotate imagej2_find_maxima_jython_script.py @ 0:93b416f8e9e3 draft
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
| author | imgteam | 
|---|---|
| date | Tue, 17 Sep 2019 17:00:19 -0400 | 
| parents | |
| children | 8c0ace7ae77c | 
| rev | line source | 
|---|---|
| 0 
93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 1 import sys | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 2 import jython_utils | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 3 from ij import ImagePlus, IJ | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 4 from ij.plugin.filter import Analyzer, MaximumFinder | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 5 from ij.process import ImageProcessor | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 6 from jarray import array | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 7 | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 8 # Fiji Jython interpreter implements Python 2.5 which does not | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 9 # provide support for argparse. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 10 error_log = sys.argv[ -10 ] | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 11 input = sys.argv[ -9 ] | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 12 scale_when_converting = jython_utils.asbool( sys.argv[ -8 ] ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 13 weighted_rgb_conversions = jython_utils.asbool( sys.argv[ -7 ] ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 14 noise_tolerance = int( sys.argv[ -6 ] ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 15 output_type = sys.argv[ -5 ] | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 16 exclude_edge_maxima = jython_utils.asbool( sys.argv[ -4 ] ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 17 light_background = jython_utils.asbool( sys.argv[ -3 ] ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 18 tmp_output_path = sys.argv[ -2 ] | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 19 output_datatype = sys.argv[ -1 ] | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 20 | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 21 # Open the input image file. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 22 input_image_plus = IJ.openImage( input ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 23 | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 24 # Create a copy of the image. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 25 input_image_plus_copy = input_image_plus.duplicate() | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 26 image_processor_copy = input_image_plus_copy.getProcessor() | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 27 bit_depth = image_processor_copy.getBitDepth() | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 28 analyzer = Analyzer( input_image_plus_copy ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 29 | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 30 try: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 31 # Set the conversion options. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 32 options = [] | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 33 # The following 2 options are applicable only to RGB images. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 34 if bit_depth == 24: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 35 if scale_when_converting: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 36 option.append( "scale" ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 37 if weighted_rgb_conversions: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 38 options.append( "weighted" ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 39 # Perform conversion - must happen even if no options are set. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 40 IJ.run( input_image_plus_copy, "Conversions...", "%s" % " ".join( options ) ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 41 if output_type in [ 'List', 'Count' ]: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 42 # W're generating a tabular file for the output. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 43 # Set the Find Maxima options. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 44 options = [ 'noise=%d' % noise_tolerance ] | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 45 if output_type.find( '_' ) > 0: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 46 output_type_str = 'output=[%s]' % output_type.replace( '_', ' ' ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 47 else: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 48 output_type_str = 'output=%s' % output_type | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 49 options.append( output_type_str ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 50 if exclude_edge_maxima: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 51 options.append( 'exclude' ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 52 if light_background: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 53 options.append( 'light' ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 54 # Run the command. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 55 IJ.run( input_image_plus_copy, "Find Maxima...", "%s" % " ".join( options ) ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 56 results_table = analyzer.getResultsTable() | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 57 results_table.saveAs( tmp_output_path ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 58 else: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 59 # Find the maxima of an image (does not find minima). | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 60 # LIMITATIONS: With output_type=Segmented_Particles | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 61 # (watershed segmentation), some segmentation lines | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 62 # may be improperly placed if local maxima are suppressed | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 63 # by the tolerance. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 64 mf = MaximumFinder() | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 65 if output_type == 'Single_Points': | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 66 output_type_param = mf.SINGLE_POINTS | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 67 elif output_type == 'Maxima_Within_Tolerance': | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 68 output_type_param = mf.IN_TOLERANCE | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 69 elif output_type == 'Segmented_Particles': | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 70 output_type_param = mf.SEGMENTED | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 71 elif output_type == 'List': | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 72 output_type_param = mf.LIST | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 73 elif output_type == 'Count': | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 74 output_type_param = mf.COUNT | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 75 # Get a new byteProcessor with a normal (uninverted) LUT where | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
 imgteam parents: diff
changeset | 76 # the marked points are set to 255 (Background 0). Pixels outside | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 77 # of the roi of the input image_processor_copy are not set. No | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 78 # output image is created for output types POINT_SELECTION, LIST | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 79 # and COUNT. In these cases findMaxima returns null. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 80 byte_processor = mf.findMaxima( image_processor_copy, | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 81 noise_tolerance, | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 82 ImageProcessor.NO_THRESHOLD, | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 83 output_type_param, | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 84 exclude_edge_maxima, | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 85 False ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 86 # Invert the image or ROI. | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 87 byte_processor.invert() | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 88 if output_type == 'Segmented_Particles' and not light_background: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 89 # Invert the values in this image's LUT (indexed color model). | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 90 byte_processor.invertLut() | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 91 image_plus = ImagePlus( "output", byte_processor ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 92 IJ.saveAs( image_plus, output_datatype, tmp_output_path ) | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 93 except Exception, e: | 
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93b416f8e9e3
"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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changeset | 94 jython_utils.handle_error( error_log, str( e ) ) | 
