Mercurial > repos > imgteam > imagej2_sharpen

annotate imagej2_analyze_skeleton_jython_script.py @ 0:a84f2cbf091b draft

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"planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
author imgteam
date Tue, 17 Sep 2019 17:08:08 -0400
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children 4c43ba752312
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a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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1 import jython_utils
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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2 import math
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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3 import sys
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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4 from ij import IJ
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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5 from sc.fiji.analyzeSkeleton import AnalyzeSkeleton_
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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6
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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7 BASIC_NAMES = [ 'Branches', 'Junctions', 'End-point Voxels',
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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8 'Junction Voxels', 'Slab Voxels', 'Average branch length',
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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9 'Triple Points', 'Quadruple Points', 'Maximum Branch Length' ]
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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10 DETAIL_NAMES = [ 'Skeleton ID', 'Branch length', 'V1 x', 'V1 y', 'V1 z', 'V2 x',
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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11 'V2 y', 'V2 z', 'Euclidean distance' ]
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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13 def get_euclidean_distance( vertex1, vertex2 ):
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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14 x1, y1, z1 = get_points( vertex1 )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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15 x2, y2, z2 = get_points( vertex2 )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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16 return math.sqrt( math.pow( ( x2 - x1 ), 2 ) +
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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17 math.pow( ( y2 - y1 ), 2 ) +
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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18 math.pow( ( z2 - z1 ), 2 ) )
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a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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20 def get_graph_length( graph ):
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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21 length = 0
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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22 for edge in graph.getEdges():
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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23 length = length + edge.getLength()
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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24 return length
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26 def get_points( vertex ):
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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27 # An array of Point, which has attributes x,y,z.
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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28 point = vertex.getPoints()[ 0 ]
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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29 return point.x, point.y, point.z
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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31 def get_sorted_edge_lengths( graph ):
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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32 # Return graph edges sorted from longest to shortest.
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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33 edges = graph.getEdges()
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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34 edges = sorted( edges, key=lambda edge: edge.getLength(), reverse=True )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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35 return edges
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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37 def get_sorted_graph_lengths( result ):
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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38 # Get the separate graphs (skeletons).
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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39 graphs = result.getGraph()
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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40 # Sort graphs from longest to shortest.
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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41 graphs = sorted( graphs, key=lambda g: get_graph_length( g ), reverse=True )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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42 return graphs
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43
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44 def save( result, output, show_detailed_info, calculate_largest_shortest_path, sep='\t' ):
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45 num_trees = int( result.getNumOfTrees() )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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46 outf = open( output, 'wb' )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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47 outf.write( '# %s\n' % sep.join( BASIC_NAMES ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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48 for index in range( num_trees ):
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49 outf.write( '%d%s' % ( result.getBranches()[ index ], sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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50 outf.write( '%d%s' % ( result.getJunctions()[ index ], sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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51 outf.write( '%d%s' % ( result.getEndPoints()[ index ], sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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52 outf.write( '%d%s' % ( result.getJunctionVoxels()[ index ], sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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53 outf.write( '%d%s' % ( result.getSlabs()[ index ], sep ) )
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54 outf.write( '%.3f%s' % ( result.getAverageBranchLength()[ index ], sep ) )
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55 outf.write( '%d%s' % ( result.getTriples()[ index ], sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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56 outf.write( '%d%s' % ( result.getQuadruples()[ index ], sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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57 outf.write( '%.3f' % result.getMaximumBranchLength()[ index ] )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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58 if calculate_largest_shortest_path:
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59 outf.write( '%s%.3f%s' % ( sep, result.shortestPathList.get( index ), sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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60 outf.write( '%d%s' % ( result.spStartPosition[ index ][ 0 ], sep ) )
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61 outf.write( '%d%s' % ( result.spStartPosition[ index ][ 1 ], sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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62 outf.write( '%d\n' % result.spStartPosition[ index ][ 2 ] )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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63 else:
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64 outf.write( '\n' )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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65 if show_detailed_info:
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66 outf.write( '# %s\n' % sep.join( DETAIL_NAMES ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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67 # The following index is a placeholder for the skeleton ID.
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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68 # The terms "graph" and "skeleton" refer to the same thing.
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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69 # Also, the SkeletonResult.java code states that the
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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70 # private Graph[] graph object is an array of graphs (one
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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71 # per tree).
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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72 for index, graph in enumerate( get_sorted_graph_lengths( result ) ):
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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73 for edge in get_sorted_edge_lengths( graph ):
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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74 vertex1 = edge.getV1()
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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75 x1, y1, z1 = get_points( vertex1 )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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76 vertex2 = edge.getV2()
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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77 x2, y2, z2 = get_points( vertex2 )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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78 outf.write( '%d%s' % ( index+1, sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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79 outf.write( '%.3f%s' % ( edge.getLength(), sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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80 outf.write( '%d%s' % ( x1, sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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81 outf.write( '%d%s' % ( y1, sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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82 outf.write( '%d%s' % ( z1, sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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83 outf.write( '%d%s' % ( x2, sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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84 outf.write( '%d%s' % ( y2, sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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85 outf.write( '%d%s' % ( z2, sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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86 outf.write( '%.3f' % get_euclidean_distance( vertex1, vertex2 ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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87 if calculate_largest_shortest_path:
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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88 # Keep number of separated items the same for each line.
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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89 outf.write( '%s %s' % ( sep, sep ) )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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90 outf.write( ' %s' % sep )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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91 outf.write( ' %s' % sep )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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92 outf.write( ' \n' )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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93 else:
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94 outf.write( '\n' )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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95 outf.close()
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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96
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97 # Fiji Jython interpreter implements Python 2.5 which does not
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98 # provide support for argparse.
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99 error_log = sys.argv[ -8 ]
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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100 input = sys.argv[ -7 ]
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101 black_background = jython_utils.asbool( sys.argv[ -6 ] )
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102 prune_cycle_method = sys.argv[ -5 ]
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103 prune_ends = jython_utils.asbool( sys.argv[ -4 ] )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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104 calculate_largest_shortest_path = jython_utils.asbool( sys.argv[ -3 ] )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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105 if calculate_largest_shortest_path:
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106 BASIC_NAMES.extend( [ 'Longest Shortest Path', 'spx', 'spy', 'spz' ] )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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107 DETAIL_NAMES.extend( [ ' ', ' ', ' ', ' ' ] )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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108 show_detailed_info = jython_utils.asbool( sys.argv[ -2 ] )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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109 output = sys.argv[ -1 ]
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110
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111 # Open the input image file.
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112 input_image_plus = IJ.openImage( input )
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113
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114 # Create a copy of the image.
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115 input_image_plus_copy = input_image_plus.duplicate()
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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116 image_processor_copy = input_image_plus_copy.getProcessor()
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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117
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118 try:
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119 # Set binary options.
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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120 options = jython_utils.get_binary_options( black_background=black_background )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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121 IJ.run( input_image_plus_copy, "Options...", options )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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122
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123 # Convert image to binary if necessary.
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124 if not image_processor_copy.isBinary():
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125 IJ.run( input_image_plus_copy, "Make Binary", "" )
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126
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127 # Run AnalyzeSkeleton
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128 analyze_skeleton = AnalyzeSkeleton_()
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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129 analyze_skeleton.setup( "", input_image_plus_copy )
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130 if prune_cycle_method == 'none':
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131 prune_index = analyze_skeleton.NONE
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132 elif prune_cycle_method == 'shortest_branch':
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133 prune_index = analyze_skeleton.SHORTEST_BRANCH
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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134 elif prune_cycle_method == 'lowest_intensity_voxel':
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135 prune_index = analyze_skeleton.LOWEST_INTENSITY_VOXEL
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136 elif prune_cycle_method == 'lowest_intensity_branch':
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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137 prune_index = analyze_skeleton.LOWEST_INTENSITY_BRANCH
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138 result = analyze_skeleton.run( prune_index,
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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139 prune_ends,
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140 calculate_largest_shortest_path,
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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141 input_image_plus_copy,
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142 True,
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143 True )
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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144 # Save the results.
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145 save( result, output, show_detailed_info, calculate_largest_shortest_path )
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146 except Exception, e:
a84f2cbf091b "planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/image_processing/imagej2 commit b08f0e6d1546caaf627b21f8c94044285d5d5b9c-dirty"
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147 jython_utils.handle_error( error_log, str( e ) )