<tool id="eurlvtecwgspt" name="EURL VTEC WGS PT" version="4.0">
    <description>workflow that performs various E. coli typing tools</description>
    <requirements>
        <requirement type="package" version="3.7">python</requirement>
        <requirement type="package" version="5.26">perl</requirement>
        <requirement type="package" version="1.7">perl-bioperl</requirement>
        <requirement type="package" version="0.39">trimmomatic</requirement>
        <requirement type="package" version="8.25">coreutils</requirement>
        <requirement type="package" version="2.3.4">bowtie2</requirement>
        <requirement type="package" version="1.3.1">samtools</requirement>
        <requirement type="package" version="1.3.1">bcftools</requirement>
        <requirement type="package" version="0.7.17">bwa</requirement>
        <requirement type="package" version="3.15">spades</requirement>
        <requirement type="package" version="2.3">skesa</requirement>
        <requirement type="package" version="0.11.9">fastqc</requirement>
        <requirement type="package" version="5.0.2">quast</requirement>
        <requirement type="package" version="3.8">muscle</requirement>
        <requirement type="package" version="2.19">mlst</requirement>
		<requirement type="package" version="1.0.1">abricate</requirement>
        <requirement type="package" version="2020.2">tbb</requirement>
    </requirements>
    <!-- basic error handling -->
    <stdio>
        <exit_code range="1:" level="fatal" description="Tool exception" />
    </stdio>
    <command>
<![CDATA[
      python
      $__tool_directory__/EURL_VTEC_WGS_PT.py --log $logfile --output $report_out --contigs $contigs --quast $quast --mlstsevenloci $mlstsevenloci
      #if str( $library.type ) == "single":
          -1 ${library.input_1} --input1_ext ${library.input_1.ext} --input1_name '${library.input_1.name}' 
          --html1 $html_file_1 --html1_id $__app__.security.encode_id($html_file_1.dataset.id) --html1_path ${html_file_1.files_path} 
          --text1 $text_file_1
      #elif str( $library.type ) == "paired":
          -1 ${library.input_1} --input1_ext ${library.input_1.ext} --input1_name '${library.input_1.name}' 
          --html1 $html_file_1 --html1_id $__app__.security.encode_id($html_file_1.dataset.id) --html1_path ${html_file_1.files_path} 
          --text1 $text_file_1
          -2 ${library.input_2} --input2_ext ${library.input_2.ext} --input2_name '${library.input_2.name}' 
          --html2 $html_file_2 --html2_id $__app__.security.encode_id($html_file_2.dataset.id) --html2_path ${html_file_2.files_path} 
          --text2 $text_file_2
      #elif str( $library.type ) == "pairedcollection":
          -1 ${library.input_pc.forward} --input1_ext ${library.input_pc.forward.ext} --input1_name '${library.input_pc.forward.name}' 
          --html1 $html_file_1 --html1_id $__app__.security.encode_id($html_file_1.dataset.id) --html1_path ${html_file_1.files_path} 
          --text1 $text_file_1
          -2 ${library.input_pc.reverse} --input2_ext ${library.input_pc.reverse.ext} --input2_name '${library.input_pc.reverse.name}' 
          --html2 $html_file_2 --html2_id $__app__.security.encode_id($html_file_2.dataset.id) --html2_path ${html_file_2.files_path} 
          --text2 $text_file_2
      #end if
      #if $serotyping:
          --serotyping
          --antigen_O $antigen_O --antigen_H $antigen_H
      #end if         
      #if $virulotyping:
          --virulotyping
          --virulotyper $virulotyper --virulotyper_id $__app__.security.encode_id($virulotyper.dataset.id)
      #end if
      #if $shigatoxintyping:
          --shigatoxintyping
          --stx $stx
      #end if
      #if $amrtyping:
          --amrtyping
          --amr $amr
          --amr_id $__app__.security.encode_id($amr.dataset.id)
      #end if
]]>
    </command>

    <inputs>
        <!-- single/paired -->
        <conditional name="library">
            <param name="type" type="select" label="Is this a single-end or paired-end library">
              <option value="single">Single-end</option>
              <option value="paired">Paired-end</option>
              <option value="pairedcollection">Paired-end collection</option>
            </param>
            <when value="single">
                <param name="input_1" format="fastqsanger" type="data" label="FASTQ file" help="Must be of datatype &quot;fastqsanger&quot;" />
            </when>
            <when value="paired">
                <param name="input_1" format="fastqsanger" type="data" label="FASTQ file #1" help="Must be of datatype &quot;fastqsanger&quot;" />
                <param name="input_2" format="fastqsanger" type="data" label="FASTQ file #2" help="Must be of datatype &quot;fastqsanger&quot;" />
            </when>
            <when value="pairedcollection">
              <param name="input_pc" type="data_collection" label="Paired-end FASTQ collection" help="Must be of datatype &quot;fastqsanger&quot;" optional="false" format="txt" collection_type="paired" />
            </when>
        </conditional>
        <param name="serotyping" type="boolean" checked="true" label="Perform Serotyping" help="" />
        <param name="virulotyping" type="boolean" checked="true" label="Perform Virulotyping" help="" />
        <param name="shigatoxintyping" type="boolean" checked="true" label="Perform Shigatoxintyping" help="" />
        <param name="amrtyping" type="boolean" checked="true" label="Perform AMR typing" help="" />
    </inputs>

    <!-- define outputs -->
    <outputs>
        <data format="html" name="html_file_1" label="${tool.name} on ${on_string}: Webpage" hidden="true" />
        <data format="txt" name="text_file_1"  label="${tool.name} on ${on_string}: RawData" hidden="true" />
        <data format="html" name="html_file_2" label="${tool.name} on ${on_string}: Webpage reverse" hidden="true">
            <filter>library['type'] == 'paired' or library['type'] == 'pairedcollection'</filter>
        </data>
        <data format="txt" name="text_file_2"  label="${tool.name} on ${on_string}: RawData reverse" hidden="true">
            <filter>library['type'] == 'paired' or library['type'] == 'pairedcollection'</filter>
        </data>
        <data format="fasta" name="contigs" label="${tool.name} on ${on_string}: contigs" />
        <data format="tsv" name="quast" label="Quast on ${on_string}"  hidden="true" />
        <data name="virulotyper" format="tabular" label="Virulotyper on ${on_string}" hidden="true">
            <filter>virulotyping</filter>
        </data>
        <data name="stx" format="tabular" label="Shiga toxin subtyper on ${on_string}" hidden="true" >
            <filter>shigatoxintyping</filter>
        </data>
        <data name="mlstsevenloci" format="tabular" label="Multi Locus on ${on_string}" hidden="true" />
        <data name="antigen_O" format="tabular" label="Antigen_O typer on ${on_string}" hidden="true">
            <filter>serotyping</filter>
        </data>
        <data name="antigen_H" format="tabular" label="Antigen_H typer on ${on_string}" hidden="true">
            <filter>serotyping</filter>
        </data>
        <data name="amr" format="tabular" label="AMR typer on ${on_string}" hidden="true">
            <filter>amrtyping</filter>
        </data>
        <data name="logfile" format="txt" label="${tool.name} on ${on_string}: log" />
        <data name="report_out" format="html" label="${tool.name} on ${on_string}: report"></data>
    </outputs>

    <tests>
        <test>
            <!-- basic test on contigs file -->
            <param name="type" value="single"/>
            <param name="input_1" value="a_reads.fastq" ftype="fastqsanger"/>
            <param name="serotyping" value="true"/>
            <output name="report_out">
                <assert_contents>
                    <has_text text="wzx_208_AF529080_O26" />
                    <has_text text="wzy_192_AF529080_O26" />
                    <has_text text="fliC_269_AY337465_H11" />
                    <has_text text="fliC_276_AY337472_H11" />
                </assert_contents>
            </output>
            <output name="antigen_O" file="antigen_O" ftype="tabular" />
            <output name="antigen_H" file="antigen_H" ftype="tabular" />
        </test>
    </tests>

    <help>
**EURL VTEC WGS PT Overview**
This tool performs various typing tools:

- Raw data quality check (FASTQC)

- Trimming (Trimmomatic)

- Assembly (SPAdes)

- Virulotyping (patho_typing tool from the INNUENDO Project)

- Multi Locus Sequence Typing (MLST 7 loci)

- Serotyping (blastn)

- Shigatoxintyping (blastn of a consensus sequence against the shiga toxin subtype database from the Statens Serum Institut SSI and Technical University of Denmark DTU)

- AMR typing (Abricate with ResFinder database)

Istituto Superiore di Sanità

European Union Reference Laboratory (EU-RL) for Escherichia coli, including Verotoxigenic E. coli (VTEC)

Developer: Arnold Knijn arnold.knijn@iss.it

The development of the Virulotyping tool has been supported by INNUENDO project (https://www.innuendoweb.org) co-funded by the European Food Safety Authority (EFSA), grant agreement GP/EFSA/AFSCO/2015/01/CT2
("New approaches in identifying and characterizing microbial and chemical hazards") and by the ONEIDA project (LISBOA-01-0145-FEDER-016417) co-funded by FEEI - “Fundos Europeus Estruturais e de Investimento” 
from “Programa Operacional Regional Lisboa 2020” and by national funds from FCT - “Fundação para a Ciência e a Tecnologia” and BacGenTrack (TUBITAK/0004/2014) 
[FCT/ Scientific and Technological Research Council of Turkey (Türkiye Bilimsel ve Teknolojik Araşrrma Kurumu, TÜBITAK)].
    </help>
    <citations>
      <citation type="bibtex">@ARTICLE{andrews_s,
            author = {Andrews, S},
            keywords = {bioinformatics, ngs, qc},
            title = {{FastQC A Quality Control tool for High Throughput Sequence Data}},
            url = {http://www.bioinformatics.babraham.ac.uk/projects/fastqc/}
        }</citation>
      <citation type="doi">10.1093/bioinformatics/btu170</citation>
      <citation type="doi">10.1186/gb-2009-10-3-r25</citation>
      <citation type="doi">10.1038/nmeth.1923</citation>
      <citation type="bibtex">@ARTICLE{seemann_t,
            author = {Seemann, T},
            keywords = {bioinformatics, ngs},
            title = {{mlst}},
            url = {https://github.com/tseemann/mlst/}
        }</citation>
      <citation type="bibtex">@ARTICLE{seemann_t,
            author = {Seemann, T},
            keywords = {bioinformatics, ngs},
            title = {{abricate}},
            url = {https://github.com/tseemann/abricate/}
        }</citation>
      <citation type="doi">10.1089/cmb.2012.0021</citation>
      <citation type="doi">10.1186/s13059-018-1540-z</citation>
      <citation type="doi">d10.1093/jac/dks261</citation>
      <citation type="doi">10.1128/JCM.00008-15</citation>
      <citation type="bibtex">@ARTICLE{andrews_s,
            author = {Li, M, Copeland, A, and Han, J},
            keywords = {bioinformatics, ngs},
            title = {{DUK – A Fast and Efficient Kmer Based Sequence Matching Tool}},
            url = {https://www.osti.gov/servlets/purl/1016000/}
        }</citation>
      <citation type="bibtex">@ARTICLE{andrews_s,
            author = {Edwards, RA},
            keywords = {bioinformatics, ngs},
            title = {{fastq-pair}},
            url = {https://github.com/linsalrob/EdwardsLab/}
        }</citation>
      <citation type="doi">10.1186/1471-2105-10-421</citation>
      <citation type="doi">10.1093/nar/gkh340</citation>
    </citations>
</tool>