Mercurial > repos > iuc > ampvis2_alpha_diversity
annotate alpha_diversity.xml @ 8:77000428c613 draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
author | iuc |
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date | Fri, 01 Nov 2024 17:09:34 +0000 |
parents | 289d6299bd2e |
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rev | line source |
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8
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
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1 <tool id="ampvis2_alpha_diversity" name="ampvis2 alpha diversity" version="@TOOL_VERSION@+galaxy1" profile="@PROFILE@" license="MIT"> |
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b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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2 <description></description> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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3 <macros> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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4 <import>macros.xml</import> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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5 </macros> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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6 <expand macro="header"/> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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7 <command detect_errors="exit_code"><![CDATA[ |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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8 Rscript '$rscript' |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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9 ]]></command> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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10 <configfiles> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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11 <configfile name="rscript"><![CDATA[ |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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12 #set m='"' + '", "'.join(str($measure).split(",")) + '"' |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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13 library(ampvis2, quietly = TRUE) |
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77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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14 |
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b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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15 d <- readRDS("$data") |
8
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
6
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16 table <- amp_alphadiv(d, |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
6
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17 measure = c($m), |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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18 richness = $richness |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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19 @RAREFY_TOKEN@, |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
6
diff
changeset
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20 ) |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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21 plot <- amp_alphadiv(d, |
0
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff
changeset
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22 measure = c($m), |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
diff
changeset
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23 richness = $richness |
8
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
6
diff
changeset
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24 @RAREFY_TOKEN@, |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
6
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changeset
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25 plot = TRUE, |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
6
diff
changeset
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26 plot_group_by = "$group_by", |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
6
diff
changeset
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27 plot_scatter = $plot_scatter |
0
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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28 ) |
8
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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29 write.table(table, file='$alphadiv', quote=FALSE, sep='\t', row.names=FALSE) |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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30 ggsave("$alphadiv_plot", |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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31 plot = plot, |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
6
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32 device = "$output_options.out_format" |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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33 #if str($output_options.plot_width) != '' |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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34 , width = $output_options.plot_width |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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35 #end if |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
6
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36 #if str($output_options.plot_height) != '' |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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37 , height = $output_options.plot_height |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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38 #end if |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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39 ) |
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b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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40 ]]></configfile> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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41 </configfiles> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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42 <inputs> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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43 <expand macro="rds_input_macro"/> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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44 <param argument="measure" type="select" multiple="true" optional="false" label="Alpha-diversity measure(s) to be included"> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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45 <option value="observed" selected="true">observed</option> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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46 <option value="shannon" selected="true">shannon</option> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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47 <option value="simpson" selected="true">simpson</option> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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48 <option value="invsimpson" selected="true">invsimpson</option> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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49 </param> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
parents:
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50 <param argument="richness" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Calculate sample richness estimates" help="Chao1 and ACE"/> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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51 <expand macro="rarefy_macro" help="before calculating alpha diversity and/or richnes"/> |
8
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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52 <param argument="group_by" type="text" label="Group by metadata field (for plotting)" help="Specify the metadata field to group the plot by"/> |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
parents:
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53 <param argument="plot_scatter" type="boolean" truevalue="TRUE" falsevalue="FALSE" label="Scatter plot instead of boxplot?" help="Select to generate a scatter plot instead of a boxplot"/> |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
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54 <expand macro="out_format_macro"/> |
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b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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55 </inputs> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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56 <outputs> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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57 <data name="alphadiv" format="tabular"/> |
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77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
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58 <expand macro="out_macro" name="alphadiv_plot" label=": plot"/> |
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b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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59 </outputs> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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60 <tests> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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61 <test> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
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62 <param name="data" value="AalborgWWTPs.rds" ftype="ampvis2"/> |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
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63 <param name="group_by" value="Plant"/> |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
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64 <param name="plot_scatter" value="false"/> |
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b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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65 <output name="alphadiv"> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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66 <assert_contents> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb30e4b8d695e096753fe7cb5056b118b25a87fd
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67 <has_line line="SampleID	Plant	Date	Year	Period	Reads	uniqueOTUs	Shannon	Simpson	invSimpson"/> |
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b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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68 <has_text text="16SAMP-536	"/> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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69 <has_text text="	Aalborg West	"/> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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70 <has_n_lines n="68"/> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
iuc
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71 <has_n_columns n="10"/> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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72 </assert_contents> |
b54f72135f58
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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73 </output> |
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74 <output name="alphadiv_plot" ftype="pdf"> |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
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75 <assert_contents> |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
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76 <has_size min="1"/> |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
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77 </assert_contents> |
77000428c613
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit cb7f20bd60570b9115fb8a9ce793dbe9b3fd65f5
iuc
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78 </output> |
0
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ampvis2 commit 7c0ecbffdb5e993f5af7e3b52c424c2761fb91d3"
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79 </test> |
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80 <test> |
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81 <param name="data" value="AalborgWWTPs.rds" ftype="ampvis2"/> |
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82 <param name="measure" value="observed,shannon"/> |
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83 <param name="richness" value="true"/> |
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84 <param name="rarefy" value="500"/> |
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85 <param name="group_by" value="Plant"/> |
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86 <param name="plot_scatter" value="true"/> |
0
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87 <output name="alphadiv"> |
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88 <assert_contents> |
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89 <has_line line="SampleID	Plant	Date	Year	Period	RawReads	Reads	uniqueOTUs	Shannon	Simpson	invSimpson	Chao1	ACE"/> |
0
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90 <has_text text="16SAMP-536	"/> |
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91 <has_text text="	Aalborg West	"/> |
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92 <has_n_lines n="68"/> |
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93 <has_n_columns n="13"/> |
0
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94 <has_text text="	500	"/> |
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95 </assert_contents> |
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96 </output> |
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97 <output name="alphadiv_plot"> |
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98 <assert_contents> |
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99 <has_size min="1"/> |
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100 </assert_contents> |
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101 </output> |
0
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102 </test> |
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103 </tests> |
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104 <help><![CDATA[ |
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105 |
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106 What it does |
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107 ============ |
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108 |
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109 Calculate alpha-diversity indices for each sample and combines with the metadata. |
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110 |
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111 The Galaxy tool calls the `amp_alpha_diversity |
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112 <https://kasperskytte.github.io/ampvis2/reference/amp_alphadiv.html>`_ function |
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113 of the ampvis2 package. |
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114 |
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115 Usage |
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116 ===== |
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117 |
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118 The alpha-diversity indices are calculated per sample using the vegan function `diversity <https://rdrr.io/pkg/vegan/man/diversity.html>`_, |
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119 where the read abundances are first rarefied using `rrarefy <https://rdrr.io/pkg/vegan/man/rarefy.html>`_ by the size of the rarefy argument. |
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120 Refer to the vegan documentation for details about the different indices and how they are |
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121 calculated. |
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122 |
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123 |
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124 Input |
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125 ===== |
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126 |
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127 @HELP_RDS_INPUT@ |
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128 |
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129 Output |
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130 ====== |
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131 |
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132 A tabular dataset containing the chosen alpha-diversity measures per sample. |
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133 |
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134 ]]></help> |
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135 <expand macro="citations"> |
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136 <citation type="doi">10.1371/journal.pcbi.1003531</citation> |
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137 </expand> |
6
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138 </tool> |