annotate clair3.xml @ 0:44f6ec903688 draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
author iuc
date Wed, 15 Jun 2022 09:45:05 +0000
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children 63e02ef6e153
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44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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1 <tool id="clair3" name="Clair3" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.09">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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2 <macros>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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3 <token name="@TOOL_VERSION@">0.1.11</token>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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4 <token name="@VERSION_SUFFIX@">0</token>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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5 </macros>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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6 <requirements>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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7 <requirement type="package" version="@TOOL_VERSION@">clair3</requirement>
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8 </requirements>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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9 <version_command><![CDATA[run_clair3.sh --version | cut -f2 -d ' ']]></version_command>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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10 <command detect_errors="exit_code"><![CDATA[
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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11 #if $ref_source.source == "history"
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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12 ln -s '${ref_source.ref_fasta}' reference.fasta &&
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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13 #elif $ref_source.source == "builtin"
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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14 ln -s '${ref_source.ref_fasta_builtin.fields.path}' reference.fasta &&
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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15 #end if
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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16 samtools faidx reference.fasta &&
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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17
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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18 #if $model_source.source == "datatable"
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19 #set model_path = $model_source.model.fields.path
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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20 #end if
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21
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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22 ln -s '${$bam_input}' input_reads.bam &&
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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23 ln -s '${$bam_input.metadata.bam_index}' input_reads.bai &&
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24
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25 #if $bed_or_vcf.bed_or_vcf_selector == 'bed'
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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26 ln -s '$bed_or_vcf.bed_fn' input.bed &&
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27 #elif $bed_or_vcf.bed_or_vcf_selector == 'vcf'
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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28 ln -s '$bed_or_vcf.vcf_fn' input.vcf &&
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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29 #end if
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30
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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31 run_clair3.sh
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32 --bam_fn=input_reads.bam
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33 --ref_fn=reference.fasta
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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34 #if $model_source.source == "datatable"
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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35 --platform='${model_source.model.fields.platform}'
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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36 --model_path=${model_source.model.fields.path}
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37 #else
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38 #if $model_source.select_built_in == "hifi" or $model_source.select_built_in == "ilmn":
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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39 --platform='${$model_source.select_built_in}'
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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40 #else
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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41 --platform='ont'
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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42 #end if
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43 --model_path=\$(dirname \$(which run_clair3.sh))/models/$model_source.select_built_in
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44 #end if
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
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45 --output='.'
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46 --threads=\${GALAXY_SLOTS:-1}
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47 #if $bed_or_vcf.bed_or_vcf_selector == 'bed'
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48 --bed_fn=input.bed
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49 #elif $bed_or_vcf.bed_or_vcf_selector == 'vcf'
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50 --vcf_fn=input.vcf
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51 #else
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52 $bed_or_vcf.include_all_ctgs
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53 #end if
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54 #if $output_options.selection_mode == 'advanced'
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55 $output_options.gvcf
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56 #end if
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57 #if $adv.qual
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58 --qual=$adv.qual
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59 #end if
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60 #if $adv.snp_min_af
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61 --snp_min_af=$adv.snp_min_af
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62 #end if
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63 #if $adv.indel_min_af
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64 --indel_min_af=$adv.indel_min_af
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65 #end if
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66 $adv.enable_phasing
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67 $adv.no_phasing_for_fa
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68 $adv.print_ref_calls
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69 $adv.ploidity_model
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70 #if $adv.chunk_size
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71 --chunk_size=$adv.chunk_size
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72 #end if
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73
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74 ]]></command>
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75 <inputs>
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76 <conditional name="ref_source">
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77 <param type="select" label="Reference genome source" name="source">
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78 <option value="history" selected="true">History</option>
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79 <option value="builtin">Built-in</option>
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80 </param>
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81 <when value="history">
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82 <param type="data" format="fasta" name="ref_fasta" label="Reference genome" />
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83 </when>
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84 <when value="builtin">
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85 <param type="select" name="ref_fasta_builtin" label="Reference genome">
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86 <options from_data_table="all_fasta" />
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87 </param>
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88 </when>
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89 </conditional>
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90 <conditional name="model_source">
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91 <param type="select" label="Select if you want to use built-in models or your own via datatable." name="source">
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92 <option value="built-in">Built-in</option>
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93 <option value="datatable">Datatable</option>
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94 </param>
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95 <when value="built-in">
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96 <param type="select" name="select_built_in" label="Select built-in model.">
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97 <option value="r941_prom_sup_g5014">r941_prom_sup_g5014</option>
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98 <option value="r941_prom_hac_g360+g422">r941_prom_hac_g360+g422</option>
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99 <option value="hifi">hifi</option>
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100 <option value="ilmn">ilmn</option>
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101 </param>
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102 </when>
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103 <when value="datatable">
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104 <param argument="--model_path" type="select" name="model" label="Select model" >
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105 <options from_data_table="clair3_models">
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106 <validator type="no_options" message="no modles available" />
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107 </options>
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108 </param>
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109 </when>
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110 </conditional>
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111 <param type="data" name="bam_input" format="bam" label="BAM file input." />
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112 <conditional name="bed_or_vcf">
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113 <param type="select" name="bed_or_vcf_selector" label="Restict variant calling to:">
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114 <option value="unrestricted" selected="true">Whole reference (unrestricted)</option>
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115 <option value="bed" selected="true">Regions defined in BED dataset</option>
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116 <option value="vcf">Sites defined in VCF dataset</option>
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117 </param>
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118 <when value="bed">
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119 <param type="data" name="bed_fn" format="bed" label="Call variants only in the provided bed regions."/>
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120 </when>
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121 <when value="vcf">
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122 <param type="data" name="vcf_fn" format="vcf" label="Candidate sites VCF file input, variants will only be called at the sites in the VCF file if provided."/>
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123 </when>
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124 <when value="unrestricted">
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125 <param type="boolean" name="include_all_ctgs" truevalue="--include_all_ctgs" falsevalue="" checked="true" label="Call variants on all contigs, otherwise call in chr{1..22,X,Y} and {1..22,X,Y}, default: disable." />
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126 </when>
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127 </conditional>
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128 <conditional name="output_options">
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129 <param label="Use advanced output options" name="selection_mode" type="select">
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130 <option selected="true" value="defaults">Show merged output file only</option>
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131 <option value="advanced">Show intermediate output files</option>
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132 </param>
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133 <when value="defaults"/>
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134 <when value="advanced">
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135 <param name="full_alignment_check" type="boolean" label="Show full alignment file"/>
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136 <param name="pileup_check" type="boolean" label="Show pileup file"/>
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137 <param name="phased_bam_check" type="boolean" label="Show itermediate phased BAM file"/>
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138 <param type="boolean" name="gvcf" truevalue="--gvcf" falsevalue="" label="Enable GVCF output, default: disable" />
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139 </when>
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140 </conditional>
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141 <section name="adv" title="Advanced Parameters" expanded="false">
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142 <param type="integer" name="qual" value="0" min="0" label="If set, variants with >qual will be marked PASS, or LowQual otherwise." />
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143 <param type="float" name="snp_min_af" value="0.08" min="0" max="1" label="Minimum SNP AF required for a candidate variant. Lowering the value might increase a bit of sensitivity in trade of speed and accuracy, default: ont:0.08,hifi:0.08,ilmn:0.08." />
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144 <param type="float" name="indel_min_af" value="0.15" min="0" max="1" label="Minimum INDEL AF required for a candidate variant. Lowering the value might increase a bit of sensitivity in trade of speed and accuracy, default: ont:0.15,hifi:0.08,ilmn:0.08." />
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145 <param type="boolean" name="enable_phasing" truevalue="--enable_phasing" falsevalue="" label="Output phased variants using whatshap, default: disable" />
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146 <param type="boolean" name="no_phasing_for_fa" truevalue="--no_phasing_for_fa" falsevalue="" label="EXPERIMENTAL: Call variants without whatshap phasing in full alignment calling, default: disable." />
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147 <param type="boolean" name="print_ref_calls" truevalue="--print_ref_calls" falsevalue="" label="Show reference calls (0/0) in vcf file, default: disable." />
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148 <param type="select" name="ploidity_model" label="Call with the following ploidy model" help="EXPERIMENTAL: Enable haploid calling mode. Only 1/1 is considered as a variant, default: disable. EXPERIMENTAL: Enable haploid calling mode. 0/1 and 1/1 are considered as a variant, default: disable.">
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149 <option value="" selected="true">diploid</option>
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150 <option value="--haploid_sensitive">haploid (sensitive)</option>
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151 <option value="--haploid_precise">haploid (precise)</option>
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152 </param>
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153 <param type="integer" name="chunk_size" value="5000000" min="1" label="The size of each chuck for parallel processing, default: 5Mbp." optional="true" />
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154 </section>
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155 </inputs>
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156 <outputs>
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157 <data name="merge_output" format="vcf_bgzip" from_work_dir="./merge_output.vcf.gz" />
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158 <data name="pileup" format="vcf_bgzip" from_work_dir="./pileup.vcf.gz">
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159 <filter>output_options['selection_mode'] == 'advanced' and output_options['pileup_check']</filter>
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160 </data>
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161 <data name="full_alignment" format="vcf_bgzip" from_work_dir="./full_alignment.vcf.gz">
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162 <filter>output_options['selection_mode'] == 'advanced' and output_options['full_alignment_check']</filter>
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163 </data>
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164 <data name="phased_bam" format="bam" from_work_dir="./phased_bam.bam">
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165 <filter>output_options['selection_mode'] == 'advanced' and output_options['phased_bam_check']</filter>
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166 </data>
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167 </outputs>
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168 <tests>
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169 <test expect_num_outputs="1">
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170 <conditional name="model_source">
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171 <param name="source" value="built-in"/>
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172 <param name="select_built_in" value="r941_prom_hac_g360+g422"/>
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173 </conditional>
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174 <param name="bam_input" value="test1.bam" />
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175 <conditional name="ref_source">
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176 <param name="source" value="history" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
177 <param name="ref_fasta" value="test1.fasta" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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178 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
179 <conditional name="bed_or_vcf">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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180 <param name="bed_or_vcf_selector" value="vcf"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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181 <param name="vcf_fn" value="test1.vcf.gz" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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182 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
183 <conditional name="output_options">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
184 <param name="selection_mode" value="advanced"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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185 <param name="gvcf" value="true" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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186 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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187 <section name="adv">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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188 <param name="no_phasing_for_fa" value="true"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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189 <param name="print_ref_calls" value="true"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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190 <param name="ploidity_model" value=""/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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191 </section>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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192 <assert_stdout>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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193 <has_text text="[WARNING] No contig intersection found, output header only in" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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194 </assert_stdout>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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195 </test>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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196 <test expect_num_outputs="4">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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197 <conditional name="model_source">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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198 <param name="source" value="builtin" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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199 <param name="select_built_in" value="r941_prom_hac_g360+g422" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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200 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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201 <param name="bam_input" value="test1.bam" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
202 <conditional name="bed_or_vcf">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
203 <param name="bed_or_vcf_selector" value="unrestricted"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
204 <param name="include_all_ctgs" value="true" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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205 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
206 <conditional name="ref_source">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
207 <param name="source" value="builtin" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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208 <param name="ref_fasta_builtin" value="test1" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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209 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
210 <conditional name="output_options">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
211 <param name="selection_mode" value="advanced"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
212 <param name="pileup_check" value="true"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
213 <param name="full_alignment_check" value="true"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
214 <param name="phased_bam_check" value="true"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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diff changeset
215 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
216 <section name="adv">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
217 <param name="print_ref_calls" value="true"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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diff changeset
218 </section>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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219 <output name="merge_output" decompress="true" file="merge_output_1.vcf" ftype="vcf_bgzip"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
220 <output name="pileup" decompress="true" file="pileup_1.vcf" ftype="vcf_bgzip"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
221 <output name="full_alignment" decompress="true" file="full_alignment_1.vcf" ftype="vcf_bgzip"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
222 <output name="phased_bam" file="phased_bam_1.bam" ftype="bam"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
223 </test>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
224 <test expect_num_outputs="4">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
225 <conditional name="model_source">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
226 <param name="source" value="datatable" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
227 <param name="model" value="test_model" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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diff changeset
228 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
229 <param name="bam_input" value="test1.bam" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
230 <conditional name="bed_or_vcf">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
231 <param name="bed_or_vcf_selector" value="bed"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
232 <param name="bed_fn" value="test1.bed" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
233 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
234 <conditional name="ref_source">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
235 <param name="source" value="builtin" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
236 <param name="ref_fasta_builtin" value="test1" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
237 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
238 <conditional name="output_options">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
239 <param name="selection_mode" value="advanced"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
240 <param name="pileup_check" value="true"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
241 <param name="full_alignment_check" value="true"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
242 <param name="phased_bam_check" value="true"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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diff changeset
243 </conditional>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
244 <section name="adv">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
245 <param name="snp_min_af" value="0.5"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
246 <param name="indel_min_af" value="0.12"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
247 <param name="no_phasing_for_fa" value="true" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
248 <param name="print_ref_calls" value="true"/>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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diff changeset
249 </section>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
250 <output name="merge_output" ftype="vcf_bgzip">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
251 <assert_contents>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
252 <has_size value="450" delta="50" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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diff changeset
253 </assert_contents>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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diff changeset
254 </output>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
255 <output name="pileup" ftype="vcf_bgzip">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
256 <assert_contents>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
257 <has_size value="0" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
258 </assert_contents>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
259 </output>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
260 <output name="full_alignment" ftype="vcf_bgzip">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
261 <assert_contents>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
262 <has_size value="0" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
263 </assert_contents>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
264 </output>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
265 <output name="phased_bam" ftype="bam">
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
266 <assert_contents>
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
diff changeset
267 <has_size value="0" />
44f6ec903688 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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268 </assert_contents>
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269 </output>
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270 </test>
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271 </tests>
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272 <help><![CDATA[
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273 Clair3 is a germline small variant caller for long-reads. Clair3 makes the best of two major method categories:
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274 pileup calling handles most variant candidates with speed, and full-alignment tackles complicated candidates to maximize precision and recall.
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275 Clair3 runs fast and has superior performance, especially at lower coverage. Clair3 is simple and modular for easy deployment and integration.
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276
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277 https://github.com/HKU-BAL/Clair3
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278
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279 LICENSE:
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280
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281 Copyright 2021 The University of Hong Kong, Department of Computer Science
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282
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283 Redistribution and use in source and binary forms, with or without modification,
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284 are permitted provided that the following conditions are met:
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285
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286 1. Redistributions of source code must retain the above copyright notice, this
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287 list of conditions and the following disclaimer.
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288
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289 2. Redistributions in binary form must reproduce the above copyright notice,
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290 this list of conditions and the following disclaimer in the documentation
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291 and/or other materials provided with the distribution.
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292
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293 3. Neither the name of the copyright holder nor the names of its contributors
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294 may be used to endorse or promote products derived from this software without
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295 specific prior written permission.
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296
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297 THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND
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298 ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED
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299 WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
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300 DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR CONTRIBUTORS BE LIABLE FOR
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301 ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES
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302 (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES;
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303 LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON
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304 ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT
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305 (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS
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306 SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.
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307 ]]></help>
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308 <citations>
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309 <citation type="doi">10.1101/2021.12.29.474431</citation>
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310 </citations>
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311 </tool>