Mercurial > repos > iuc > clair3
annotate clair3.xml @ 0:44f6ec903688 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
author | iuc |
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date | Wed, 15 Jun 2022 09:45:05 +0000 |
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children | 63e02ef6e153 |
rev | line source |
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0
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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1 <tool id="clair3" name="Clair3" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="21.09"> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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2 <macros> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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3 <token name="@TOOL_VERSION@">0.1.11</token> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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4 <token name="@VERSION_SUFFIX@">0</token> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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changeset
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5 </macros> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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6 <requirements> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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7 <requirement type="package" version="@TOOL_VERSION@">clair3</requirement> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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8 </requirements> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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9 <version_command><![CDATA[run_clair3.sh --version | cut -f2 -d ' ']]></version_command> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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10 <command detect_errors="exit_code"><![CDATA[ |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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changeset
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11 #if $ref_source.source == "history" |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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12 ln -s '${ref_source.ref_fasta}' reference.fasta && |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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13 #elif $ref_source.source == "builtin" |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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14 ln -s '${ref_source.ref_fasta_builtin.fields.path}' reference.fasta && |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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15 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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16 samtools faidx reference.fasta && |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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17 |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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18 #if $model_source.source == "datatable" |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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19 #set model_path = $model_source.model.fields.path |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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20 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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21 |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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22 ln -s '${$bam_input}' input_reads.bam && |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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23 ln -s '${$bam_input.metadata.bam_index}' input_reads.bai && |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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24 |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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25 #if $bed_or_vcf.bed_or_vcf_selector == 'bed' |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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26 ln -s '$bed_or_vcf.bed_fn' input.bed && |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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27 #elif $bed_or_vcf.bed_or_vcf_selector == 'vcf' |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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28 ln -s '$bed_or_vcf.vcf_fn' input.vcf && |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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29 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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30 |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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31 run_clair3.sh |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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32 --bam_fn=input_reads.bam |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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33 --ref_fn=reference.fasta |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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changeset
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34 #if $model_source.source == "datatable" |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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35 --platform='${model_source.model.fields.platform}' |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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36 --model_path=${model_source.model.fields.path} |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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37 #else |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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38 #if $model_source.select_built_in == "hifi" or $model_source.select_built_in == "ilmn": |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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39 --platform='${$model_source.select_built_in}' |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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40 #else |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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41 --platform='ont' |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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42 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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43 --model_path=\$(dirname \$(which run_clair3.sh))/models/$model_source.select_built_in |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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44 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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45 --output='.' |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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46 --threads=\${GALAXY_SLOTS:-1} |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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47 #if $bed_or_vcf.bed_or_vcf_selector == 'bed' |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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48 --bed_fn=input.bed |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
parents:
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49 #elif $bed_or_vcf.bed_or_vcf_selector == 'vcf' |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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50 --vcf_fn=input.vcf |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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51 #else |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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52 $bed_or_vcf.include_all_ctgs |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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53 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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54 #if $output_options.selection_mode == 'advanced' |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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55 $output_options.gvcf |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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56 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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57 #if $adv.qual |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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58 --qual=$adv.qual |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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59 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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60 #if $adv.snp_min_af |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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61 --snp_min_af=$adv.snp_min_af |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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62 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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63 #if $adv.indel_min_af |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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64 --indel_min_af=$adv.indel_min_af |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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65 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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66 $adv.enable_phasing |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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67 $adv.no_phasing_for_fa |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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68 $adv.print_ref_calls |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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69 $adv.ploidity_model |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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70 #if $adv.chunk_size |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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71 --chunk_size=$adv.chunk_size |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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72 #end if |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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73 |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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74 ]]></command> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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75 <inputs> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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76 <conditional name="ref_source"> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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77 <param type="select" label="Reference genome source" name="source"> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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78 <option value="history" selected="true">History</option> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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79 <option value="builtin">Built-in</option> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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80 </param> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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81 <when value="history"> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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82 <param type="data" format="fasta" name="ref_fasta" label="Reference genome" /> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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83 </when> |
44f6ec903688
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/clair3 commit 9968295e33bad5323ac9cbff8f64c2db35189a5f
iuc
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84 <when value="builtin"> |
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85 <param type="select" name="ref_fasta_builtin" label="Reference genome"> |
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86 <options from_data_table="all_fasta" /> |
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87 </param> |
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88 </when> |
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89 </conditional> |
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90 <conditional name="model_source"> |
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91 <param type="select" label="Select if you want to use built-in models or your own via datatable." name="source"> |
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92 <option value="built-in">Built-in</option> |
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93 <option value="datatable">Datatable</option> |
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94 </param> |
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95 <when value="built-in"> |
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96 <param type="select" name="select_built_in" label="Select built-in model."> |
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97 <option value="r941_prom_sup_g5014">r941_prom_sup_g5014</option> |
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98 <option value="r941_prom_hac_g360+g422">r941_prom_hac_g360+g422</option> |
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99 <option value="hifi">hifi</option> |
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100 <option value="ilmn">ilmn</option> |
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101 </param> |
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102 </when> |
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103 <when value="datatable"> |
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104 <param argument="--model_path" type="select" name="model" label="Select model" > |
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105 <options from_data_table="clair3_models"> |
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106 <validator type="no_options" message="no modles available" /> |
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107 </options> |
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108 </param> |
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109 </when> |
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110 </conditional> |
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111 <param type="data" name="bam_input" format="bam" label="BAM file input." /> |
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112 <conditional name="bed_or_vcf"> |
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113 <param type="select" name="bed_or_vcf_selector" label="Restict variant calling to:"> |
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114 <option value="unrestricted" selected="true">Whole reference (unrestricted)</option> |
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115 <option value="bed" selected="true">Regions defined in BED dataset</option> |
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116 <option value="vcf">Sites defined in VCF dataset</option> |
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117 </param> |
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118 <when value="bed"> |
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119 <param type="data" name="bed_fn" format="bed" label="Call variants only in the provided bed regions."/> |
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120 </when> |
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121 <when value="vcf"> |
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122 <param type="data" name="vcf_fn" format="vcf" label="Candidate sites VCF file input, variants will only be called at the sites in the VCF file if provided."/> |
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123 </when> |
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124 <when value="unrestricted"> |
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125 <param type="boolean" name="include_all_ctgs" truevalue="--include_all_ctgs" falsevalue="" checked="true" label="Call variants on all contigs, otherwise call in chr{1..22,X,Y} and {1..22,X,Y}, default: disable." /> |
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126 </when> |
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127 </conditional> |
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128 <conditional name="output_options"> |
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129 <param label="Use advanced output options" name="selection_mode" type="select"> |
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130 <option selected="true" value="defaults">Show merged output file only</option> |
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131 <option value="advanced">Show intermediate output files</option> |
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132 </param> |
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133 <when value="defaults"/> |
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134 <when value="advanced"> |
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135 <param name="full_alignment_check" type="boolean" label="Show full alignment file"/> |
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136 <param name="pileup_check" type="boolean" label="Show pileup file"/> |
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137 <param name="phased_bam_check" type="boolean" label="Show itermediate phased BAM file"/> |
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138 <param type="boolean" name="gvcf" truevalue="--gvcf" falsevalue="" label="Enable GVCF output, default: disable" /> |
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139 </when> |
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140 </conditional> |
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141 <section name="adv" title="Advanced Parameters" expanded="false"> |
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142 <param type="integer" name="qual" value="0" min="0" label="If set, variants with >qual will be marked PASS, or LowQual otherwise." /> |
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143 <param type="float" name="snp_min_af" value="0.08" min="0" max="1" label="Minimum SNP AF required for a candidate variant. Lowering the value might increase a bit of sensitivity in trade of speed and accuracy, default: ont:0.08,hifi:0.08,ilmn:0.08." /> |
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144 <param type="float" name="indel_min_af" value="0.15" min="0" max="1" label="Minimum INDEL AF required for a candidate variant. Lowering the value might increase a bit of sensitivity in trade of speed and accuracy, default: ont:0.15,hifi:0.08,ilmn:0.08." /> |
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145 <param type="boolean" name="enable_phasing" truevalue="--enable_phasing" falsevalue="" label="Output phased variants using whatshap, default: disable" /> |
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146 <param type="boolean" name="no_phasing_for_fa" truevalue="--no_phasing_for_fa" falsevalue="" label="EXPERIMENTAL: Call variants without whatshap phasing in full alignment calling, default: disable." /> |
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147 <param type="boolean" name="print_ref_calls" truevalue="--print_ref_calls" falsevalue="" label="Show reference calls (0/0) in vcf file, default: disable." /> |
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148 <param type="select" name="ploidity_model" label="Call with the following ploidy model" help="EXPERIMENTAL: Enable haploid calling mode. Only 1/1 is considered as a variant, default: disable. EXPERIMENTAL: Enable haploid calling mode. 0/1 and 1/1 are considered as a variant, default: disable."> |
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149 <option value="" selected="true">diploid</option> |
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150 <option value="--haploid_sensitive">haploid (sensitive)</option> |
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151 <option value="--haploid_precise">haploid (precise)</option> |
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152 </param> |
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153 <param type="integer" name="chunk_size" value="5000000" min="1" label="The size of each chuck for parallel processing, default: 5Mbp." optional="true" /> |
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154 </section> |
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155 </inputs> |
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156 <outputs> |
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157 <data name="merge_output" format="vcf_bgzip" from_work_dir="./merge_output.vcf.gz" /> |
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158 <data name="pileup" format="vcf_bgzip" from_work_dir="./pileup.vcf.gz"> |
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159 <filter>output_options['selection_mode'] == 'advanced' and output_options['pileup_check']</filter> |
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160 </data> |
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161 <data name="full_alignment" format="vcf_bgzip" from_work_dir="./full_alignment.vcf.gz"> |
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162 <filter>output_options['selection_mode'] == 'advanced' and output_options['full_alignment_check']</filter> |
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163 </data> |
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164 <data name="phased_bam" format="bam" from_work_dir="./phased_bam.bam"> |
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165 <filter>output_options['selection_mode'] == 'advanced' and output_options['phased_bam_check']</filter> |
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166 </data> |
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167 </outputs> |
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168 <tests> |
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169 <test expect_num_outputs="1"> |
44f6ec903688
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170 <conditional name="model_source"> |
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171 <param name="source" value="built-in"/> |
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172 <param name="select_built_in" value="r941_prom_hac_g360+g422"/> |
44f6ec903688
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173 </conditional> |
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174 <param name="bam_input" value="test1.bam" /> |
44f6ec903688
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175 <conditional name="ref_source"> |
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176 <param name="source" value="history" /> |
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177 <param name="ref_fasta" value="test1.fasta" /> |
44f6ec903688
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178 </conditional> |
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179 <conditional name="bed_or_vcf"> |
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180 <param name="bed_or_vcf_selector" value="vcf"/> |
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181 <param name="vcf_fn" value="test1.vcf.gz" /> |
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182 </conditional> |
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183 <conditional name="output_options"> |
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184 <param name="selection_mode" value="advanced"/> |
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185 <param name="gvcf" value="true" /> |
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186 </conditional> |
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187 <section name="adv"> |
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188 <param name="no_phasing_for_fa" value="true"/> |
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189 <param name="print_ref_calls" value="true"/> |
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190 <param name="ploidity_model" value=""/> |
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191 </section> |
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192 <assert_stdout> |
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193 <has_text text="[WARNING] No contig intersection found, output header only in" /> |
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194 </assert_stdout> |
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195 </test> |
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196 <test expect_num_outputs="4"> |
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197 <conditional name="model_source"> |
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198 <param name="source" value="builtin" /> |
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199 <param name="select_built_in" value="r941_prom_hac_g360+g422" /> |
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200 </conditional> |
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201 <param name="bam_input" value="test1.bam" /> |
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202 <conditional name="bed_or_vcf"> |
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203 <param name="bed_or_vcf_selector" value="unrestricted"/> |
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204 <param name="include_all_ctgs" value="true" /> |
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205 </conditional> |
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206 <conditional name="ref_source"> |
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207 <param name="source" value="builtin" /> |
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208 <param name="ref_fasta_builtin" value="test1" /> |
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209 </conditional> |
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210 <conditional name="output_options"> |
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211 <param name="selection_mode" value="advanced"/> |
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212 <param name="pileup_check" value="true"/> |
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213 <param name="full_alignment_check" value="true"/> |
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214 <param name="phased_bam_check" value="true"/> |
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215 </conditional> |
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216 <section name="adv"> |
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217 <param name="print_ref_calls" value="true"/> |
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218 </section> |
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219 <output name="merge_output" decompress="true" file="merge_output_1.vcf" ftype="vcf_bgzip"/> |
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220 <output name="pileup" decompress="true" file="pileup_1.vcf" ftype="vcf_bgzip"/> |
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221 <output name="full_alignment" decompress="true" file="full_alignment_1.vcf" ftype="vcf_bgzip"/> |
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222 <output name="phased_bam" file="phased_bam_1.bam" ftype="bam"/> |
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223 </test> |
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224 <test expect_num_outputs="4"> |
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225 <conditional name="model_source"> |
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226 <param name="source" value="datatable" /> |
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227 <param name="model" value="test_model" /> |
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228 </conditional> |
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229 <param name="bam_input" value="test1.bam" /> |
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230 <conditional name="bed_or_vcf"> |
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231 <param name="bed_or_vcf_selector" value="bed"/> |
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232 <param name="bed_fn" value="test1.bed" /> |
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233 </conditional> |
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234 <conditional name="ref_source"> |
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235 <param name="source" value="builtin" /> |
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236 <param name="ref_fasta_builtin" value="test1" /> |
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237 </conditional> |
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238 <conditional name="output_options"> |
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239 <param name="selection_mode" value="advanced"/> |
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240 <param name="pileup_check" value="true"/> |
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241 <param name="full_alignment_check" value="true"/> |
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242 <param name="phased_bam_check" value="true"/> |
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243 </conditional> |
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244 <section name="adv"> |
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245 <param name="snp_min_af" value="0.5"/> |
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246 <param name="indel_min_af" value="0.12"/> |
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247 <param name="no_phasing_for_fa" value="true" /> |
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248 <param name="print_ref_calls" value="true"/> |
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249 </section> |
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250 <output name="merge_output" ftype="vcf_bgzip"> |
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251 <assert_contents> |
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252 <has_size value="450" delta="50" /> |
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253 </assert_contents> |
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254 </output> |
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255 <output name="pileup" ftype="vcf_bgzip"> |
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256 <assert_contents> |
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257 <has_size value="0" /> |
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258 </assert_contents> |
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259 </output> |
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260 <output name="full_alignment" ftype="vcf_bgzip"> |
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261 <assert_contents> |
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262 <has_size value="0" /> |
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263 </assert_contents> |
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264 </output> |
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265 <output name="phased_bam" ftype="bam"> |
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266 <assert_contents> |
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267 <has_size value="0" /> |
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268 </assert_contents> |
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269 </output> |
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270 </test> |
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271 </tests> |
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272 <help><![CDATA[ |
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273 Clair3 is a germline small variant caller for long-reads. Clair3 makes the best of two major method categories: |
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274 pileup calling handles most variant candidates with speed, and full-alignment tackles complicated candidates to maximize precision and recall. |
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275 Clair3 runs fast and has superior performance, especially at lower coverage. Clair3 is simple and modular for easy deployment and integration. |
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276 |
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277 https://github.com/HKU-BAL/Clair3 |
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278 |
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279 LICENSE: |
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280 |
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281 Copyright 2021 The University of Hong Kong, Department of Computer Science |
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282 |
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283 Redistribution and use in source and binary forms, with or without modification, |
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284 are permitted provided that the following conditions are met: |
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285 |
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286 1. Redistributions of source code must retain the above copyright notice, this |
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287 list of conditions and the following disclaimer. |
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288 |
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289 2. Redistributions in binary form must reproduce the above copyright notice, |
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290 this list of conditions and the following disclaimer in the documentation |
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291 and/or other materials provided with the distribution. |
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292 |
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293 3. Neither the name of the copyright holder nor the names of its contributors |
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294 may be used to endorse or promote products derived from this software without |
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295 specific prior written permission. |
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296 |
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297 THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND |
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298 ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED |
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299 WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE |
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300 DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR CONTRIBUTORS BE LIABLE FOR |
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301 ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES |
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302 (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; |
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303 LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON |
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304 ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT |
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305 (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS |
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306 SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. |
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307 ]]></help> |
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308 <citations> |
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309 <citation type="doi">10.1101/2021.12.29.474431</citation> |
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310 </citations> |
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311 </tool> |