Mercurial > repos > iuc > dropletutils
annotate dropletutils.xml @ 4:1797cbe967d1 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 49840f36c6623746bb7f8eb64bf4bf0cdae9c24e"
| author | iuc |
|---|---|
| date | Thu, 05 Dec 2019 01:32:29 -0500 |
| parents | f0de368eabca |
| children | cdf4443d5625 |
| rev | line source |
|---|---|
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
diff
changeset
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1 <?xml version="1.0" encoding="utf-8"?> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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changeset
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2 <tool id="dropletutils" name="DropletUtils" version="@PACKAGE_VERSION@+@GALAXY_VERSION@" > |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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3 <description>Utilities for handling droplet-based single-cell RNA-seq data</description> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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4 <macros> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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5 <token name="@PACKAGE_VERSION@" >1.2.1</token> |
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4
1797cbe967d1
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 49840f36c6623746bb7f8eb64bf4bf0cdae9c24e"
iuc
parents:
3
diff
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6 <token name="@GALAXY_VERSION@" >5</token> |
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0
4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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changeset
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7 <token name="@TXIN@">tenx.input</token> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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8 <token name="@TXOUT@">tenx.output</token> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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9 <xml name="test_dirin" > |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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10 <conditional name="tenx_format"> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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11 <param name="use" value="directory" /> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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12 <param name="input" value="in_matrix.mtx" /> |
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f0de368eabca
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit a4cee4cac5097029188d836d5b904b605dbb943d"
iuc
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13 <param name="input_barcodes" value="in_barcodes.tsv" /> |
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0
4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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14 <param name="input_genes" value="in_genes.tsv" /> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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15 </conditional> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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16 </xml> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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17 </macros> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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18 <requirements> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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19 <requirement type="package" version="@PACKAGE_VERSION@">bioconductor-dropletutils</requirement> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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20 <requirement type="package" version="1.2_17" >r-matrix</requirement> |
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1
cfe1e6c28d95
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 40d4a92c62d75fe931baba8657cde006a26d84cf"
iuc
parents:
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21 <requirement type="package" version="1.10.1" >bioconductor-scater</requirement> |
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1797cbe967d1
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 49840f36c6623746bb7f8eb64bf4bf0cdae9c24e"
iuc
parents:
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22 <requirement type="package" version="1">fonts-conda-ecosystem</requirement> |
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0
4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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23 </requirements> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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24 <version_command><![CDATA[ |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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25 Rscript -e 'packageVersion("DropletUtils")' | grep -oP '\d\.\d\.\d' |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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26 ]]> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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changeset
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27 </version_command> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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28 <command detect_errors='exit_code'><![CDATA[ |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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29 #if str($tenx_format.use) == 'directory': |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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30 mkdir '@TXIN@' && |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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31 ln -s '$tenx_format.input' '@TXIN@/matrix.mtx' && |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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32 ln -s '$tenx_format.input_genes' '@TXIN@/genes.tsv' && |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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33 ln -s '$tenx_format.input_barcodes' '@TXIN@/barcodes.tsv' && |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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34 #end if |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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35 |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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36 #if str($operation.use) == 'filter': |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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37 #if str($operation.outformat) == 'directory': |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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38 mkdir '@TXOUT@' && |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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39 #end if |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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40 #end if |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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41 |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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42 Rscript '$__tool_directory__/scripts/dropletutils.Rscript' '$droplet_conf' |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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43 ]]> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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44 </command> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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45 <configfiles> |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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46 <configfile name="droplet_conf" > |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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47 ## defaults |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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48 empty.fdr_threshold = 0.01 |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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49 eparams=formals(emptyDrops) |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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50 dparams=formals(defaultDrops) |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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51 bparams=formals(barcodeRanks) |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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52 |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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53 ## File params |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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54 in.type='$tenx_format.use' |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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55 out.type=NULL |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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56 |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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57 files=list() |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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58 files\$table='$table' |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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59 files\$plot='$plot' |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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60 files\$out=NULL |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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61 |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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62 #if str($tenx_format.use) == 'directory': |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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63 files\$infile='@TXIN@' |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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64 #else |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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65 files\$infile='$input' |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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66 #end if |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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67 |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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68 #if str($operation.use) == 'filter': |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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69 #if str($operation.method.use) == 'defaultdrops': |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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70 do.method="defaultDrops" |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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71 dparams\$expected=as.integer('$operation.method.expected') |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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72 dparams\$upper.quant=as.numeric('$operation.method.upperquant') |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
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73 dparams\$lower.prop=as.numeric('$operation.method.lowerprop') |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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74 #else if str($operation.method.use) == 'emptydrops': |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
iuc
parents:
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75 do.method="emptyDrops" |
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4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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76 eparams\$lower=as.integer('$operation.method.lower') |
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77 empty.fdr_threshold=as.numeric('$operation.method.fdr_thresh') |
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78 #end if |
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79 |
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80 out.type='$operation.outformat' |
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81 #if str($operation.outformat) == 'directory': |
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82 files\$out='@TXOUT@' |
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83 #else if str($operation.outformat) == 'h5ad': |
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84 files\$out='$fileout_h5ad' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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85 #else if str($operation.outformat) == 'tsv': |
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86 files\$out='$fileout_tsv' |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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87 #end if |
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88 |
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89 #else if str($operation.use) == 'barcode_rank': |
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90 do.method="barcodeRankings" |
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91 bparams\$lower=as.integer('$operation.lower') |
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92 #end if |
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93 |
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94 seed.val=as.integer('$seed') |
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95 |
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96 </configfile> |
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97 </configfiles> |
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98 <inputs> |
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99 <conditional name="tenx_format"> |
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100 <param name="use" type="select" label="Format for the input matrix"> |
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101 <option value="directory" >Bundled (barcodes.tsv, genes.tsv, matrix.mtx)</option> |
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102 <option value="h5ad" selected="true" >AnnData/H5</option> |
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103 <option value="tsv">Tabular</option> |
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104 </param> |
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105 <when value="directory"> |
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106 <param name="input" type="data" format="mtx,txt" label="Count Data"/> |
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107 <param name="input_genes" type="data" format="tabular,tsv,txt" label="Genes List"/> |
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108 <param name="input_barcodes" type="data" format="tabular,tsv,txt" label="Barcodes List"/> |
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109 </when> |
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110 <when value="h5ad"> |
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111 <param name="input" type="data" format="h5ad" label="Count Data"/> |
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112 </when> |
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113 <when value="tsv"> |
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114 <param name="input" type="data" format="tabular,tsv" label="Count Data" help="The first column must contain gene names, and the first row must contain barcode identifiers." /> |
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115 </when> |
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116 </conditional> |
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117 <conditional name="operation" > |
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118 <param name="use" type="select" label="Operation" help="The filtering uses intelligent methods to generate output 10X matrices as would be otherwise generated by CellRanger. The ranking option serves to illustrate the number of detected barcodes in the sample."> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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119 <option value="filter" selected="true">Filter for Barcodes</option> |
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120 <option value="barcode_rank">Rank Barcodes</option> |
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121 </param> |
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122 <when value="filter"> |
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123 <conditional name="method"> |
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124 <param name="use" type="select" label="Method" help="The DefaultDrops method calls cells according to the number of UMIs associated with each barcode as implemented by CellRanger. The EmptyDrops method identifies empty droplets using a smart ambient background model." > |
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125 <option value="defaultdrops" selected="true">DefaultDrops</option> |
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126 <option value="emptydrops">EmptyDrops</option> |
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127 </param> |
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128 <when value="defaultdrops"> |
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129 <param name="expected" type="integer" min="1" value="3000" label="Expected Number of Cells" help="The expected number of cells in the sample." /> |
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130 <param name="upperquant" type="float" min="0" max="1.0" value="0.99" label="Upper Quantile" help="The quantile of the top expected barcodes to consider." /> |
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131 <param name="lowerprop" type="float" min="0" max="1.0" value="0.1" label="Lower Proportion" help="The fraction of molecules of the upper quantile that must be exceeded for a cell barcode to be detected." /> |
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0
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132 </when> |
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133 <when value="emptydrops"> |
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134 <param name="lower" type="integer" min="1" value="100" label="Lower-bound Threshold" help="The lower-bound threshold of the total UMI count at which barcodes beneath this are assumed to be empty droplets." /> |
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135 <param name="fdr_thresh" type="float" min="0" max="1" value="0.01" label="FDR Threshold" help="False Discovery Rate threshold at which droplets with significant deviations from the ambient profile are detected at this threshold. If set to 0, the threshold is ignored." /> |
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0
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136 </when> |
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137 </conditional> |
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138 <param name="outformat" type="select" label="Format for output matrices"> |
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139 <option value="directory" >Bundled (barcodes.tsv, genes.tsv, matrix.mtx)</option> |
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140 <option value="h5ad" selected="true" >AnnData/H5</option> |
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141 <option value="tsv">Tabular</option> |
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142 </param> |
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143 </when> |
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144 <when value="barcode_rank"> |
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145 <param name="lower" type="integer" min="1" value="100" label="Lower Bound" help="Lower bound on the total UMI count, at or below which all barcodes are assumed to correspond to empty droplets."/> |
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0
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146 </when> |
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147 </conditional> |
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148 <param name="seed" type="integer" value="100" label="Random Seed" /> |
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149 </inputs> |
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150 <outputs> |
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151 <data name="fileout_tsv" format="tsv" label="${tool.name} Count Matrix on ${on_string}"> |
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152 <filter>operation['use']=='filter' and operation['outformat'] == 'tsv' </filter> |
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153 </data> |
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154 <data name="fileout_h5ad" format="h5ad" label="${tool.name} AnnData on ${on_string}"> |
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155 <filter>operation['use']=='filter' and operation['outformat'] == 'h5ad'</filter> |
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156 </data> |
|
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157 <data name="barcodes_out" format="tsv" from_work_dir="@TXOUT@/barcodes.tsv" label="${tool.name} 10X Barcodes on ${on_string}" > |
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158 <filter>operation['use']=='filter' and operation['outformat'] == 'directory'</filter> |
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159 </data> |
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160 <data name="genes_out" format="tsv" from_work_dir="@TXOUT@/genes.tsv" label="${tool.name} 10X Genes on ${on_string}" > |
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161 <filter>operation['use']=='filter' and operation['outformat'] == 'directory'</filter> |
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162 </data> |
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163 <data name="matrix_out" format="mtx" from_work_dir="@TXOUT@/matrix.mtx" label="${tool.name} 10X Matrices on ${on_string}" > |
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164 <filter>operation['use']=='filter' and operation['outformat'] == 'directory'</filter> |
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165 </data> |
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166 <data name="plot" format="png" label="${tool.name} Plot on ${on_string}"> |
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167 <filter>(operation['use']=='filter' and operation['method']['use']=="emptydrops") or operation['use']=='barcode_rank'</filter> |
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168 </data> |
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169 <data name="table" format="tsv" label="${tool.name} Table on ${on_string}"> |
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170 <filter>operation['use']=='filter' and operation['method']['use']=="emptydrops"</filter> |
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171 </data> |
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172 </outputs> |
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173 <tests> |
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174 <!-- Directory input tests --> |
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175 <!-- ::: Default Drops --> |
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176 <test expect_num_outputs="1"> |
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177 <expand macro="test_dirin" /> |
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178 <conditional name="operation"> |
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179 <param name="use" value="filter" /> |
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180 <param name="outformat" value="h5ad" /><!-- H5AD --> |
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181 </conditional> |
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182 <output name="fileout_h5ad" value="defs_defaultdrops.h5ad" compare="sim_size" delta="10" /> |
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183 </test> |
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184 <test expect_num_outputs="1"> |
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185 <expand macro="test_dirin" /> |
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186 <conditional name="operation"> |
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187 <param name="use" value="filter" /> |
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188 <param name="outformat" value="tsv" /><!-- TSV --> |
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189 </conditional> |
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190 <output name="fileout_tsv" > |
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191 <assert_contents> |
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192 <has_n_columns n="1026" /> |
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193 <has_text_matching expression="^\sTCGCCAAGAA\sGGACTTAGAA\sTGCGCCTGAA\sACGGACACAA\sGCGGACACAA" /> |
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194 <has_text_matching expression="GENE50\s4\s0\s0\s0\s0\s5\s11\s8\s5" /> |
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195 <has_text_matching expression="GENE100\s6\s3\s5\s0\s0\s16\s31\s15\s26" /> |
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196 </assert_contents> |
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197 </output> |
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198 </test> |
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199 <!-- :: Barcode Ranks --> |
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200 <test expect_num_outputs="1"> |
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201 <expand macro="test_dirin" /> |
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202 <conditional name="operation"> |
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203 <param name="use" value="barcode_rank" /> |
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204 <param name="lower" value="120" /> |
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205 </conditional> |
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4
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 49840f36c6623746bb7f8eb64bf4bf0cdae9c24e"
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206 <output name="plot" value="defs_barcoderankings.png" compare="sim_size" delta="400"/> |
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0
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207 </test> |
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208 <!-- ::: Empty Drops --> |
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209 <test expect_num_outputs="3"> |
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210 <expand macro="test_dirin" /> |
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211 <conditional name="operation"> |
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212 <param name="use" value="filter" /> |
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213 <param name="outformat" value="h5ad" /> |
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214 <conditional name="method"> |
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215 <param name="use" value="emptydrops" /> |
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216 <param name="lower" value="150" /> |
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217 <param name="fdr_thresh" value="0.02" /> |
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218 </conditional> |
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219 </conditional> |
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220 <output name="fileout_h5ad" value="defs_emptydrops_150_0002.h5ad" compare="sim_size" delta="10" /> |
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221 <output name="table" > |
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222 <assert_contents> |
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223 <has_n_columns n="9" /> |
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224 <has_line_matching expression="^\sbar.names\sTotal\sLogProb\sPValue\sLimited\sFDR\sis.Cell\sis.CellAndLimited" /> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 4d89eb1eb951ef094d1f77c46824d9c38be4445b"
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225 <has_line_matching expression="^994\sGGCATTACAA\s338\s-246.922772388055\s9.99900009999e-05\sTRUE\s9.99900009999e-05\sTRUE\sTRUE" /> |
|
a8aa294401be
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 4d89eb1eb951ef094d1f77c46824d9c38be4445b"
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226 <has_line_matching expression="^998\sCATGAAGCAA\s151\s-166.644236503983\s9.99900009999e-05\sTRUE\s9.99900009999e-05\sTRUE\sTRUE" /> |
|
0
4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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227 </assert_contents> |
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228 </output> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 49840f36c6623746bb7f8eb64bf4bf0cdae9c24e"
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229 <output name="plot" value="defs_emptydrops_150_0002.png" compare="sim_size" delta="400" /> |
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0
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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230 </test> |
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231 <!-- Other format input tests --> |
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232 <!-- ::: Empty Drops, same as above but input is h5ad --> |
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233 <test expect_num_outputs="3"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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234 <conditional name="tenx_format" > |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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235 <param name="use" value="h5ad" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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236 <param name="input" value="in_matrix.h5ad" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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237 </conditional> |
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238 <conditional name="operation"> |
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239 <param name="use" value="filter" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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240 <param name="outformat" value="h5ad" /> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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241 <conditional name="method"> |
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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242 <param name="use" value="emptydrops" /> |
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243 <param name="lower" value="150" /> |
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244 <param name="fdr_thresh" value="0.02" /> |
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245 </conditional> |
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246 </conditional> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 49840f36c6623746bb7f8eb64bf4bf0cdae9c24e"
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247 <output name="fileout_h5ad" value="defs_emptydrops_150_0002a.h5ad" compare="sim_size" delta="400" /> |
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248 <output name="table" > |
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249 <assert_contents> |
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250 <has_n_columns n="9" /> |
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251 <has_line_matching expression="^\sbar.names\sTotal\sLogProb\sPValue\sLimited\sFDR\sis.Cell\sis.CellAndLimited" /> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 4d89eb1eb951ef094d1f77c46824d9c38be4445b"
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252 <has_line_matching expression="^1100\sCCGGAAGCAA\s169\s-198.117943099773\s9.99900009999e-05\sTRUE\s0.000126279506880773\sTRUE\sTRUE" /> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 4d89eb1eb951ef094d1f77c46824d9c38be4445b"
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253 <has_line_matching expression="^1114\sTCCGAAGCAA\s182\s-196.181449214729\s9.99900009999e-05\sTRUE\s0.000126279506880773\sTRUE\sTRUE" /> |
|
0
4cd9f0008d9c
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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254 </assert_contents> |
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255 </output> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit 49840f36c6623746bb7f8eb64bf4bf0cdae9c24e"
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256 <output name="plot" value="defs_emptydrops_150_0002a.png" compare="sim_size" delta="400" /> |
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0
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/dropletutils/ commit e66ab3d4fc0c1a72523e8f93447cc07cdd6816b7
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257 </test> |
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258 <!-- Output a directory --> |
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259 <test expect_num_outputs="3"> |
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260 <conditional name="tenx_format" > |
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261 <param name="use" value="tsv" /> |
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262 <param name="input" value="in_matrix.tsv" /> |
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263 </conditional> |
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264 <conditional name="operation"> |
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265 <param name="use" value="filter" /> |
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266 <param name="outformat" value="directory" /> |
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267 <conditional name="method"> |
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268 <param name="use" value="defaultdrops" /> |
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269 <param name="expected" value="2000" /> |
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270 <param name="upperquant" value="0.85" /> |
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271 <param name="lowerpopr" value="0.2" /> |
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272 </conditional> |
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273 </conditional> |
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274 <output name="genes_out" file="in_genes.tsv" ftype="tsv" /><!-- same as the input in this case --> |
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275 <output name="barcodes_out" ftype="tsv" > |
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276 <assert_contents> |
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277 <has_n_columns n="1" /> |
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278 <has_line line="GGAAGAAGAA" /> |
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279 <has_line line="TCGAATGGAA" /> |
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280 </assert_contents> |
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281 </output> |
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282 <output name="matrix_out" ftype="mtx" > |
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283 <assert_contents> |
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284 <has_line_matching expression="^100\s2650\s64072$" /> |
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285 <has_line_matching expression="^19\s1\s1$" /> |
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286 <has_line_matching expression="^100\s2650\s1$" /> |
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287 </assert_contents> |
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288 </output> |
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289 </test> |
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290 </tests> |
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291 <help><![CDATA[ |
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292 Utilities to process 10X data in a variety of formats. Methods include: |
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293 |
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294 *Filter data:* |
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295 |
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296 * **DefaultDrops** emulates the native 10X method to produce a filtered count matrix |
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297 * **EmptyDrops** is a more informative quantile-based approach to produce a filtered count matrix. |
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298 |
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299 *Metrics:* |
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300 |
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301 * **RankBarcodes** produces a gap statistic plot to estimate the number of barcodes detected in the sample matrix. |
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302 |
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303 *Background* |
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304 |
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305 Droplet-based single-cell RNA sequencing (scRNA-seq) technologies allow researchers to obtain transcriptome-wide expression profiles for thousands of cells at once. Briefly, each cell is encapsulated in a droplet in a oil-water emulsion, along with a bead containing reverse transcription primers with a unique barcode sequence. After reverse transcription inside the droplet, each cell’s cDNA is labelled with that barcode (referred to a “cell barcode”). Bursting of the droplets yields a pool of cDNA for library preparation and sequencing. Debarcoding of the sequences can then be performed to obtain the expression profile for each cell. |
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306 |
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307 This tool implements some general utilities for handling these data after quantification of expression. In particular, we focus on the 10X Genomics platform, providing functions to load in the matrix of unique molecule identifier (UMI) counts as well as the raw molecule information. Functions are also available for downsampling the UMI count matrix or the raw reads; for distinguishing cells from empty droplets, based on the UMI counts; and to eliminate the effects of barcode swapping on Illumina 4000 sequencing machine. |
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308 ]]></help> |
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309 <citations> |
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310 <citation type="doi">10.1186/s13059-019-1662-y</citation> |
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311 </citations> |
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312 </tool> |